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公开(公告)号：US20060105371A1
公开(公告)日：2006-05-18
申请号：US11261198
申请日：2005-10-27
申请人： Ye Fang , Fang Lai , Laurent Picard , Brian Webb
发明人： Ye Fang , Fang Lai , Laurent Picard , Brian Webb
CPC分类号： C12N5/0068 , C12N2503/00 , C12N2535/10
摘要： Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.
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公开(公告)号：US20050009204A1
公开(公告)日：2005-01-13
申请号：US10639718
申请日：2003-08-12
申请人： Ye Fang , Yulong Hong , Joydeep Lahiri , Fang Lai , Jinlin Peng , Brian Webb , Ann Ferrie
发明人： Ye Fang , Yulong Hong , Joydeep Lahiri , Fang Lai , Jinlin Peng , Brian Webb , Ann Ferrie
IPC分类号： C40B30/04 , G01N33/68 , G01N33/74 , G01N33/543
CPC分类号： C40B30/04 , G01N33/74 , G01N2333/726
摘要： A thematic microarray and methods for multiplexed binding assays using a cocktail solution of labeled ligands in the presence or absence of a target compound is provided. The methods enables researchers to screening compounds against multiple targets using a microarray format.
摘要翻译： 提供了一种专题微阵列和在存在或不存在目标化合物的情况下使用标记配体的混合溶液进行多重结合测定的方法。 这些方法使研究人员可以使用微阵列格式来筛选多个靶点的化合物。
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公开(公告)号：US20060147993A1
公开(公告)日：2006-07-06
申请号：US11026371
申请日：2004-12-30
申请人： Alain Carre , Alexander Efrenov , Ye Fang , Yulong Hong , Valerie Lacarriere , Joydeep Lahiri , Fang Lai , John Mauro , Srikanth Raghavan , Brian Webb
发明人： Alain Carre , Alexander Efrenov , Ye Fang , Yulong Hong , Valerie Lacarriere , Joydeep Lahiri , Fang Lai , John Mauro , Srikanth Raghavan , Brian Webb
CPC分类号： G01N33/554 , G01N33/74 , G01N2333/726
摘要： The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of a porous substrate, the membrane spanning across a plurality of pores on the porous substrate to form a plurality of cavities having sufficient geometry to permit entry of assay reagents into each cavity, thereby allowing access of assay reagents to both sides of GPCR in the membrane.
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公开(公告)号：US08293484B2
公开(公告)日：2012-10-23
申请号：US12893389
申请日：2010-09-29
申请人： Ye Fang , Fang Lai , Laurent A. G. Picard , Brian L. Webb
发明人： Ye Fang , Fang Lai , Laurent A. G. Picard , Brian L. Webb
IPC分类号： G01N33/53
CPC分类号： C12N5/0068 , C12N2503/00 , C12N2535/10
摘要： Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.
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公开(公告)号：US20120083428A1
公开(公告)日：2012-04-05
申请号：US12893389
申请日：2010-09-29
申请人： Ye Fang , Fang Lai , Laurent A.G. Picard , Brian L. Webb
发明人： Ye Fang , Fang Lai , Laurent A.G. Picard , Brian L. Webb
CPC分类号： C12N5/0068 , C12N2503/00 , C12N2535/10
摘要： Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.
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公开(公告)号：US07105347B2
公开(公告)日：2006-09-12
申请号：US10208894
申请日：2002-07-30
申请人： Ye Fang , Fang Lai , Laurent A. G. Picard , Brian L. Webb
发明人： Ye Fang , Fang Lai , Laurent A. G. Picard , Brian L. Webb
CPC分类号： C12N5/0068 , C12N2503/00 , C12N2535/10
摘要： Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.
摘要翻译： 提供了将表面介导的蛋白质递送到活细胞中并制备蛋白质转染的细胞簇阵列的方法。 该方法包括提供含蛋白质的混合物; 将所述含蛋白质的混合物在限定的位置沉积在表面上; 将含蛋白质的混合物作为微滴固定在表面上; 并以足够的密度和将蛋白质递送到细胞中的条件将细胞电镀在表面上。 含蛋白质的混合物包含任何合适的氨基酸序列，包括肽，蛋白质，蛋白质结构域，抗体或蛋白质 - 核酸缀合物等与载体试剂。 蛋白质转染的细胞阵列可用于在活细胞的天然环境中快速和直接地筛选蛋白质或酶功能或任何给定的细胞内蛋白质相互作用，以及用于其他生物和化学分析物的高通量筛选，其中 影响这些蛋白质的功能。
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公开(公告)号：US20090093371A1
公开(公告)日：2009-04-09
申请号：US12330660
申请日：2008-12-09
申请人： Alain R.E. Carre , Alexander M. Efremov , Ye Fang , Yulong Hong , Valerie Lacarriere , Joydeep Lahiri , Fang Lai , John C. Mauro , Srikanth Raghavan , Brian L. Webb
发明人： Alain R.E. Carre , Alexander M. Efremov , Ye Fang , Yulong Hong , Valerie Lacarriere , Joydeep Lahiri , Fang Lai , John C. Mauro , Srikanth Raghavan , Brian L. Webb
IPC分类号： C40B30/00
CPC分类号： G01N33/554 , G01N33/74 , G01N2333/726
摘要： The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of a porous substrate, the membrane spanning across a plurality of pores on the porous substrate to form a plurality of cavities having sufficient geometry to permit entry of assay reagents into each cavity, thereby allowing access of assay reagents to both sides of GPCR in the membrane.
摘要翻译： 本发明涉及用于GPCR的结构或功能分析的多孔基质上的G蛋白偶联受体（GPCR）微阵列，以及制备用于接收包含GPCR的膜的多孔底物表面的方法。 在一个实施方案中，本发明的GPCR微阵列包括粘附到多孔基底的上表面的膜，所述膜横跨多孔基底上的多个孔以形成具有足够几何形状的多个空腔以允许测定试剂进入 进入每个空腔，从而允许测定试剂进入膜中GPCR两侧。
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公开(公告)号：US08288113B2
公开(公告)日：2012-10-16
申请号：US12893412
申请日：2010-09-29
申请人： Ye Fang , Fang Lai , Laurent A. G. Picard , Brian L. Webb
发明人： Ye Fang , Fang Lai , Laurent A. G. Picard , Brian L. Webb
IPC分类号： G01N33/53
CPC分类号： C12N5/0068 , C12N2503/00 , C12N2535/10
摘要： Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.
摘要翻译： 提供了将表面介导的蛋白质递送到活细胞中并制备蛋白质转染的细胞簇阵列的方法。 该方法包括提供含蛋白质的混合物; 将所述含蛋白质的混合物在限定的位置沉积在表面上; 将含蛋白质的混合物作为微滴固定在表面上; 并以足够的密度和将蛋白质递送到细胞中的条件将细胞电镀在表面上。 含蛋白质的混合物包含任何合适的氨基酸序列，包括肽，蛋白质，蛋白质结构域，抗体或蛋白质 - 核酸缀合物等与载体试剂。 蛋白质转染的细胞阵列可用于在活细胞的天然环境中快速和直接地筛选蛋白质或酶功能或任何给定的细胞内蛋白质相互作用，以及用于其他生物和化学分析物的高通量筛选，其中 影响这些蛋白质的功能。
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公开(公告)号：US07473533B2
公开(公告)日：2009-01-06
申请号：US11026371
申请日：2004-12-30
申请人： Alain R. E. Carre , Alexander M. Efremov , Ye Fang , Yulong Hong , Valerie Lacarriere , Joydeep Lahiri , Fang Lai , John C. Mauro , Srikanth Raghavan , Brian L. Webb
发明人： Alain R. E. Carre , Alexander M. Efremov , Ye Fang , Yulong Hong , Valerie Lacarriere , Joydeep Lahiri , Fang Lai , John C. Mauro , Srikanth Raghavan , Brian L. Webb
IPC分类号： G01N17/04 , G01N33/566 , C12Q1/00 , C07K14/705
CPC分类号： G01N33/554 , G01N33/74 , G01N2333/726
摘要： The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of a porous substrate, the membrane spanning across a plurality of pores on the porous substrate to form a plurality of cavities having sufficient geometry to permit entry of assay reagents into each cavity, thereby allowing access of assay reagents to both sides of GPCR in the membrane.
摘要翻译： 本发明涉及用于GPCR的结构或功能分析的多孔基质上的G蛋白偶联受体（GPCR）微阵列，以及制备用于接收包含GPCR的膜的多孔底物表面的方法。 在一个实施方案中，本发明的GPCR微阵列包括粘附到多孔基底的上表面的膜，所述膜横跨多孔基底上的多个孔以形成具有足够几何形状的多个空腔以允许测定试剂进入 进入每个腔，从而允许测定试剂进入膜中GPCR两侧。
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公开(公告)号：US20120088695A1
公开(公告)日：2012-04-12
申请号：US12893412
申请日：2010-09-29
申请人： Ye Fang , Fang Lai , Laurent A.G. Picard , Brian L. Webb
发明人： Ye Fang , Fang Lai , Laurent A.G. Picard , Brian L. Webb
CPC分类号： C12N5/0068 , C12N2503/00 , C12N2535/10
摘要： Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.
摘要翻译： 提供了将表面介导的蛋白质递送到活细胞中并制备蛋白质转染的细胞簇阵列的方法。 该方法包括提供含蛋白质的混合物; 将所述含蛋白质的混合物在限定的位置沉积在表面上; 将含蛋白质的混合物作为微滴固定在表面上; 并以足够的密度和将蛋白质递送到细胞中的条件将细胞电镀在表面上。 含蛋白质的混合物包含任何合适的氨基酸序列，包括肽，蛋白质，蛋白质结构域，抗体或蛋白质 - 核酸缀合物等与载体试剂。 蛋白质转染的细胞阵列可用于在活细胞的天然环境中快速和直接地筛选蛋白质或酶功能或任何给定的细胞内蛋白质相互作用，以及用于其他生物和化学分析物的高通量筛选，其中 影响这些蛋白质的功能。
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