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1.发明授权Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains 有权构建产氨酸的细菌菌株的方法，以及用构建的产生氨基酸的菌株通过发酵制备氨基酸的方法公开(公告)号：US07608437B2
公开(公告)日：2009-10-27
申请号：US11218433
申请日：2005-09-06
申请人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12P13/14 , C12Q1/48 , C12Q1/32 , C12Q1/68 , C12N9/02 , C12N9/10 , C12N1/21 , C12P21/00 , C12N15/00 , C07K14/00 , C07H21/00
摘要： A method of producing coryneform bacteria having an improved amino acid or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium so that it is close to a consensus sequence or introducing a change in the promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on the chromosome of a coryneform bacterium by gene recombination so that it is close to a consensus sequence, obtaining mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows one of skill in the art to construct a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield, by the recombination or mutation.
摘要翻译： 具有改进的氨基酸或核酸生产力的棒状杆菌细菌的方法包括以下步骤：将氨基酸或核酸 - 生物合成基因的启动子序列中的突变引入棒状细菌的染色体上，使其接近 共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化，使得其接近共有序列，获得棒状杆菌型氨基酸的突变体或 产生核酸的微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。 该方法允许本领域技术人员通过重组或突变来构建能够富集或控制预期基因的表达而不使用质粒并以高产率促进氨基酸产生的突变体。
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2.发明申请Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains 有权构建产氨酸的细菌菌株的方法，以及用构建的产生氨基酸的菌株通过发酵制备氨基酸的方法公开(公告)号：US20060003424A1
公开(公告)日：2006-01-05
申请号：US11218433
申请日：2005-09-06
申请人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
摘要： A method of producing coryneform bacteria having an improved amino acid- or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid d- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method can construct a mutant capable of suitably enriching or controling the expression of an intended gene without using a plasmid and also capable of producing amino acids in a high yield, by the recombination or mutation.
摘要翻译： 具有改善的氨基酸或核酸生产力的棒状细菌的方法包括以下步骤：在棒状细菌的染色体上的氨基酸或核酸 - 生物合成基因的启动子序列中引入突变，使其接近 共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化，使其接近共有序列，获得棒状杆菌氨基酸d- 或产生核酸的微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。 该方法可以构建能够适当地富集或控制预期基因的表达而不使用质粒并且还能够通过重组或突变以高产率产生氨基酸的突变体。
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3.发明授权Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains 有权构建产氨酸的细菌菌株的方法，以及用构建的产生氨基酸的菌株通过发酵制备氨基酸的方法公开(公告)号：US06962805B2
公开(公告)日：2005-11-08
申请号：US09577005
申请日：2000-05-25
申请人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12N15/37 , C12N1/21 , C12N15/67 , C12P13/04 , C12P13/14 , C07H21/04 , C12N1/20 , C12N15/74 , C12Q1/32
摘要： A method of producing coryneform bacteria having improved amino acid or nucleic acid productivity comprising the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence, or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and selecting a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows the construction of a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield by recombination or mutation.
摘要翻译： 制备具有改善的氨基酸或核酸生产力的棒状细菌的方法包括以下步骤：在棒状细菌染色体上的氨基酸或核酸 - 生物合成基因的启动子序列中引入突变，使其接近共有序列 或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化使其接近共有序列，以获得棒状细胞氨基酸或核酸的突变体 培养微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。 该方法允许构建能够富集或控制预期基因的表达而不使用质粒的突变体，并通过重组或突变促进高产量的氨基酸的产生。
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4.发明授权Arginine repressor deficient strain of coryneform bacterium and method for producing L-arginine 有权棒状杆菌型精氨酸阻遏物缺陷菌株及L-精氨酸生产方法公开(公告)号：US07419810B2
公开(公告)日：2008-09-02
申请号：US11451347
申请日：2006-06-13
申请人： Mikiko Suga , Yoko Asakura , Yukiko Mori , Hisao Ito , Osamu Kurahashi
发明人： Mikiko Suga , Yoko Asakura , Yukiko Mori , Hisao Ito , Osamu Kurahashi
CPC分类号： C07K14/34
摘要： L-Arginine is produced by culturing a coryneform bacterium in which an arginine repressor involved in L-arginine biosynthesis is deleted by disrupting a gene coding for the repressor, and which has L-arginine producing ability in a medium to produce and accumulate L-arginine in the medium, and collecting the L-arginine from the medium.
摘要翻译： L-精氨酸是通过培养通过破坏编码阻遏物的基因而缺失参与L-精氨酸生物合成的精氨酸阻遏物的棒状细菌产生的，并且在培养基中具有L-精氨酸生产能力以产生和积累L-精氨酸 在培养基中，并从培养基中收集L-精氨酸。
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5.发明授权Arginine repressor deficient strain of coryneform bacterium and method for producing L-arginine 有权棒状杆菌型精氨酸阻遏物缺陷菌株及L-精氨酸生产方法公开(公告)号：US07160705B2
公开(公告)日：2007-01-09
申请号：US09835381
申请日：2001-04-17
申请人： Mikiko Suga , Yoko Asakura , Yukiko Mori , Hisao Ito , Osamu Kurahashi
发明人： Mikiko Suga , Yoko Asakura , Yukiko Mori , Hisao Ito , Osamu Kurahashi
CPC分类号： C07K14/34
摘要： L-Arginine is produced by culturing a coryneform bacterium in which an arginine repressor involved in L-arginine biosynthesis is deleted by disrupting a gene coding for the repressor, and which has L-arginine producing ability in a medium to produce and accumulate L-arginine in the medium, and collecting the L-arginine from the medium.
摘要翻译： L-精氨酸是通过培养通过破坏编码阻遏物的基因而缺失参与L-精氨酸生物合成的精氨酸阻遏物的棒状细菌产生的，并且在培养基中具有L-精氨酸生产能力以产生和积累L-精氨酸 在培养基中，并从培养基中收集L-精氨酸。
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6.发明申请公开(公告)号：US20070031946A1
公开(公告)日：2007-02-08
申请号：US11451347
申请日：2006-06-13
申请人： Mikiko Suga , Yoko Asakura , Yukiko Mori , Hisao Ito , Osamu Kurahashi
发明人： Mikiko Suga , Yoko Asakura , Yukiko Mori , Hisao Ito , Osamu Kurahashi
CPC分类号： C07K14/34
摘要： L-Arginine is produced by culturing a coryneform bacterium in which an arginine repressor involved in L-arginine biosynthesis is deleted by disrupting a gene coding for the repressor, and which has L-arginine producing ability in a medium to produce and accumulate L-arginine in the medium, and collecting the L-arginine from the medium.
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公开(公告)号：US20060293513A1
公开(公告)日：2006-12-28
申请号：US11245030
申请日：2005-10-07
申请人： Mikiko Suga , Yoko Asakura , Masakazu Sugimoto , Hisao Ito
发明人： Mikiko Suga , Yoko Asakura , Masakazu Sugimoto , Hisao Ito
CPC分类号： C12Y301/03003 , C12N9/16 , C12P13/06
摘要： The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment. (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.
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公开(公告)号：US07329523B2
公开(公告)日：2008-02-12
申请号：US11245030
申请日：2005-10-07
申请人： Mikiko Suga , Yoko Asakura , Masakazu Sugimoto , Hisao Ito
发明人： Mikiko Suga , Yoko Asakura , Masakazu Sugimoto , Hisao Ito
CPC分类号： C12Y301/03003 , C12N9/16 , C12P13/06
摘要： The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment. (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.
摘要翻译： 本发明提供了编码下列（A）或（B）中定义的蛋白质的DNA，其通过克隆将大肠杆菌的SerB缺陷复杂化的DNA片段作为DNA片段的开放阅读框，从黄色短杆菌染色体DNA文库获得 。 （A）包含序列表中SEQ ID：2的氨基酸序列的蛋白质; 或（B）包含序列表中SEQ ID NO：2的氨基酸序列中的一个或几个氨基酸的取代，缺失，插入，添加或倒位，并具有磷酸丝氨酸磷酸酶活性的氨基酸序列的蛋白质。
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公开(公告)号：US07029896B2
公开(公告)日：2006-04-18
申请号：US10321382
申请日：2002-12-18
申请人： Mikiko Suga , Yoko Asakura , Masakazu Sugimoto , Hisao Ito
发明人： Mikiko Suga , Yoko Asakura , Masakazu Sugimoto , Hisao Ito
CPC分类号： C12Y301/03003 , C12N9/16 , C12P13/06
摘要： The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment.(A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or(B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.
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公开(公告)号：US06395528B1
公开(公告)日：2002-05-28
申请号：US09761716
申请日：2001-01-18
申请人： Mikiko Suga , Yoko Asakura , Masakazu Sugimoto , Hisao Ito
发明人： Mikiko Suga , Yoko Asakura , Masakazu Sugimoto , Hisao Ito
IPC分类号： C12N916
CPC分类号： C12Y301/03003 , C12N9/16 , C12P13/06
摘要： The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment. (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.
摘要翻译： 本发明提供了编码下列（A）或（B）中定义的蛋白质的DNA，其通过克隆将大肠杆菌的SerB缺陷复杂化的DNA片段作为DNA片段的开放阅读框，从黄色短杆菌染色体DNA文库获得 （A）包含序列表中SEQ ID：2的氨基酸序列的蛋白质; 或（B）包含序列表中SEQ ID NO：2的氨基酸序列中的一个或几个氨基酸的取代，缺失，插入，添加或倒位，并具有磷酸丝氨酸磷酸酶活性的氨基酸序列的蛋白质。
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