公开(公告)号：US20090162907A1

公开(公告)日：2009-06-25

申请号：US12266921

申请日：2008-11-07

申请人： Hiroshi IZUI ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

发明人： Hiroshi IZUI ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

IPC分类号： C12P13/14

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01

摘要： A microorganism which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH; and a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

摘要翻译： 在含有饱和浓度的L-谷氨酸和碳源的液体培养基中，能够在特定pH下代谢碳源的微生物，并且具有以超过相应于饱和浓度的量累积L-谷氨酸的能力 在液体介质中的pH值; 以及通过发酵生产L-谷氨酸的方法，其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物以产生并积累L-谷氨酸并将L-谷氨酸沉淀， 培养基中的谷氨酸。

公开(公告)号：US20050196846A1

公开(公告)日：2005-09-08

申请号：US11070085

申请日：2005-03-03

申请人： Yoshihiko Hara ,  Hiroshi Izui ,  Hisao Ito

发明人： Yoshihiko Hara ,  Hiroshi Izui ,  Hisao Ito

IPC分类号： C07K14/245 ,  C07K14/27 ,  C07K14/34 ,  C12N1/20 ,  C12P13/14 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12P13/14 ,  C07K14/34

摘要： L-Glutamic acid is produced by culturing a microorganism in which an expression of L-glutamic acid-export gene, a yhfK gene, is enhanced or overexpressed, in a medium to produce and cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

摘要翻译： 通过培养在培养基中培养L-谷氨酸输出基因，yhfK基因的表达增强或过表达的微生物来产生L-谷氨酸以产生和导致培养基中L-谷氨酸的积累，以及 从培养基中收集L-谷氨酸。

公开(公告)号：US08129151B2

公开(公告)日：2012-03-06

申请号：US11624080

申请日：2007-01-17

申请人： Mika Moriya ,  Hiroshi Izui ,  Eiji Ono ,  Kazuhiko Matsui ,  Hisao Ito ,  Yoshihiko Hara

发明人： Mika Moriya ,  Hiroshi Izui ,  Eiji Ono ,  Kazuhiko Matsui ,  Hisao Ito ,  Yoshihiko Hara

IPC分类号： C12N1/00 ,  C12N1/12 ,  C12N1/20 ,  C12P1/04 ,  C12P13/04 ,  C12P13/14

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01 ,  C12R1/425 ,  Y10S435/822 ,  Y10S435/88

摘要： L-Glutamic acid is produced by culturing in a liquid culture medium a microorganism belonging to the genus Pantoea or Serratia and having an ability to produce L-glutamic acid, which increases in an activity of enzyme catalyzing a reaction for L-glutamic acid biosynthesis or which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, and collecting produced L-glutamic acid from the culture medium.

摘要翻译： L-谷氨酸通过在液体培养基中培养属于泛酸属或沙雷氏菌属的具有产生L-谷氨酸能力的微生物产生，其增加了催化L-谷氨酸生物合成反应的酶的活性，或 其催化从L-谷氨酸生物合成途径分支的反应的酶的活性降低或缺乏，并产生除L-谷氨酸以外的化合物，并从培养基中收集L-谷氨酸。

公开(公告)号：US07015010B1

公开(公告)日：2006-03-21

申请号：US09641892

申请日：2000-08-18

申请人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

发明人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

IPC分类号： C12Q1/04

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01

摘要： A microorganism which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH; and a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

摘要翻译： 在含有饱和浓度的L-谷氨酸和碳源的液体培养基中，能够在特定pH下代谢碳源的微生物，并且具有以超过相应于饱和浓度的量累积L-谷氨酸的能力 在液体介质中的pH值; 以及通过发酵生产L-谷氨酸的方法，其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物以产生并积累L-谷氨酸并将L-谷氨酸沉淀， 培养基中的谷氨酸。

公开(公告)号：USRE42350E1

公开(公告)日：2011-05-10

申请号：US12429421

申请日：2009-04-24

申请人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

发明人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

IPC分类号： C12P13/14 ,  C12N15/00 ,  C12N1/20 ,  C12N1/36 ,  C12N15/63

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01

摘要： A microorganism is provided which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and which has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH. Also provided is a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

摘要翻译： 提供了能够在饱和浓度和碳源的含有L-谷氨酸的液体培养基中在特定pH下代谢碳源的微生物，并且具有将L-谷氨酸的积累量超过对应于 在液体介质中的饱和浓度在pH值。 还提供了通过发酵生产L-谷氨酸的方法，其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物以产生并积累L-谷氨酸并沉淀 培养基中的L-谷氨酸。

公开(公告)号：US07344874B2

公开(公告)日：2008-03-18

申请号：US11070085

申请日：2005-03-03

申请人： Yoshihiko Hara ,  Hiroshi Izui ,  Hisao Ito

发明人： Yoshihiko Hara ,  Hiroshi Izui ,  Hisao Ito

IPC分类号： C12N1/20 ,  C12P13/14

CPC分类号： C12P13/14 ,  C07K14/34

摘要： L-Glutamic acid is produced by culturing a microorganism in which an expression of L-glutamic acid-export gene, a yhfK gene, is enhanced or overexpressed, in a medium to produce and cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

摘要翻译： 通过培养在培养基中培养L-谷氨酸输出基因，yhfK基因的表达增强或过表达的微生物来产生L-谷氨酸以产生和导致培养基中L-谷氨酸的积累，以及 从培养基中收集L-谷氨酸。

公开(公告)号：US07208296B2

公开(公告)日：2007-04-24

申请号：US11150265

申请日：2005-06-13

申请人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

发明人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

IPC分类号： C12P13/14 ,  C12N15/00 ,  C12N1/20 ,  C12N1/36 ,  C12N15/63

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01

摘要： A microorganism is provided which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and which has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH. Also provided is a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

摘要翻译： 提供了能够在饱和浓度和碳源的含有L-谷氨酸的液体培养基中在特定pH下代谢碳源的微生物，并且具有将L-谷氨酸的积累量超过对应于 在液体介质中的饱和浓度在pH值。 还提供了通过发酵生产L-谷氨酸的方法，其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物以产生并积累L-谷氨酸并沉淀 培养基中的L-谷氨酸。

公开(公告)号：US07785845B2

公开(公告)日：2010-08-31

申请号：US11844559

申请日：2007-08-24

申请人： Yoshihiko Hara ,  Hiroshi Izui ,  Hisao Ito

发明人： Yoshihiko Hara ,  Hiroshi Izui ,  Hisao Ito

IPC分类号： C12P13/14 ,  C12N1/20

CPC分类号： C12P13/14 ,  C07K14/34

摘要： L-Glutamic acid is produced by culturing a microorganism in which an expression of L-glutamic acid-export gene, a yhfK gene, is enhanced or overexpressed, in a medium to produce and cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

摘要翻译： 通过培养在培养基中培养L-谷氨酸输出基因yhfK基因，yhfK基因的表达增强或过表达的微生物产生并引起培养基中L-谷氨酸的积累而产生L-谷氨酸，以及 从培养基中收集L-谷氨酸。

公开(公告)号：US20090263874A1

公开(公告)日：2009-10-22

申请号：US11624080

申请日：2007-01-17

申请人： Mika Moriya ,  Hiroshi Izui ,  Eiji Ono ,  Kazuhiko Matsui ,  Hisao Ito ,  Yoshihiko Hara

发明人： Mika Moriya ,  Hiroshi Izui ,  Eiji Ono ,  Kazuhiko Matsui ,  Hisao Ito ,  Yoshihiko Hara

IPC分类号： C12P13/14

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01 ,  C12R1/425 ,  Y10S435/822 ,  Y10S435/88

摘要： L-Glutamic acid is produced by culturing in a liquid culture medium a microorganism belonging to the genus Enterobacter or Serratia and having an ability to produce L-glutamic acid, which increases in an activity of enzyme catalyzing a reaction for L-glutamic acid biosynthesis or which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, and collecting produced L-glutamic acid from the culture medium.

摘要翻译： L-谷氨酸通过在液体培养基中培养属于肠杆菌属或沙雷氏菌属的具有生产L-谷氨酸能力的微生物产生，其增加了催化L-谷氨酸生物合成的反应的酶的活性，或 其催化从L-谷氨酸生物合成途径分支的反应的酶的活性降低或缺乏，并产生除L-谷氨酸以外的化合物，并从培养基中收集L-谷氨酸。

公开(公告)号：US20090226981A1

公开(公告)日：2009-09-10

申请号：US11844559

申请日：2007-08-24

申请人： Yoshihiko Hara ,  Hiroshi Izui ,  Hisao Ito

发明人： Yoshihiko Hara ,  Hiroshi Izui ,  Hisao Ito

IPC分类号： C12P13/14 ,  C12N1/21 ,  C12N15/31

CPC分类号： C12P13/14 ,  C07K14/34

摘要： L-Glutamic acid is produced by culturing a microorganism in which an expression of L-glutamic acid-export gene, a yhfK gene, is enhanced or overexpressed, in a medium to produce and cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

摘要翻译： 通过培养在培养基中培养L-谷氨酸输出基因yhfK基因，yhfK基因的表达增强或过表达的微生物产生并引起培养基中L-谷氨酸的积累而产生L-谷氨酸，以及 从培养基中收集L-谷氨酸。