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15 条记录 (耗时 0.015 秒)

    Heat-resistant mannose isomerase, process for producing the same and process for producing mannose by using the same
    5.
    发明授权
    Heat-resistant mannose isomerase, process for producing the same and process for producing mannose by using the same 失效
    耐热甘露糖异构酶，其制造方法以及使用该方法生产甘露糖的方法

    公开(公告)号：US06897047B1

    公开(公告)日：2005-05-24

    申请号：US09959563

    申请日：2000-04-21

    申请人： Yoshiyuki Takasaki

    发明人： Yoshiyuki Takasaki

    IPC分类号： C12N9/90 ,  C12P19/24 ,  C12P19/02

    CPC分类号： C12N9/90 ,  C12P19/24 ,  C12Y503/01007

    摘要： A thermostable mannose isomerase generated from a bacterium belonging to the genus Agrobacterium, excellent in thermostability, capable of being continuously used at a temperature of 55° C. to 60° C. for a prolonged period of time, and has, for example, the following enzymatic properties: (a) function: converts D-mannose to D-fructose and vice versa; (b) substrate specificity: isomerizes the aldoses D-mannose and D-lyxose to their corresponding kitoses, but does not interact with D-glucose, D-galactose, L-mannose, D-xylose, L-xylose, D-arabinose, L-arabinose or D-ribose; (c) optimum pH: 7.5 to 8.5; (d) optimum temperature: 55° C. to 60° C.; and (e) stable pH: 6.0 to 10.0, is provided.

    摘要翻译： 由属于农杆菌属的细菌产生的热稳定性甘露糖异构酶，耐热性优异，能够在55℃〜60℃的温度下连续使用长时间，具有例如 以下酶促性质：（a）功能：将D-甘露糖转化为D-果糖，反之亦然; （b）底物特异性：将醛糖D-甘露糖和D-赖索糖异构化成其相应的小猫，但不与D-葡萄糖，D-半乳糖，L-甘露糖，D-木糖，L-木糖，D-阿拉伯糖， L-阿拉伯糖或D-核糖; （c）最佳pH：7.5至8.5; （d）最适温度：55℃至60℃。 和（e）稳定的pH：6.0至10.0。

    Mannose isomerase and process for mannose production using it
    6.
    发明授权
    Mannose isomerase and process for mannose production using it 失效
    甘露糖异构酶和使用它的甘露糖生产方法

    公开(公告)号：US5124262A

    公开(公告)日：1992-06-23

    申请号：US661139

    申请日：1991-02-27

    申请人： Yoshiyuki Takasaki ,  Takaichi Ohya

    发明人： Yoshiyuki Takasaki ,  Takaichi Ohya

    IPC分类号： C12N9/90

    CPC分类号： C12R1/38 ,  C12N9/90

    摘要： A mannose isomerase having excellent properties for industrial use, such as high thermal stability and resistance to high substrate concentrations, can be produced by culturing a strain of Pseudomonas (sp. AM-9582), and extracting it from the cells of AM-9582. Mannose can be effectively produced from fructose of high concentrations using the enzyme.

    摘要翻译： 可以通过培养假单胞菌（SP.AM-9582）菌株并从AM-9582的细胞中提取具有优异工业性质的甘露糖异构酶，例如高热稳定性和高底物浓度。 使用酶可以从高浓度的果糖有效地制备甘露糖。

    Pullulanase, methods of producing pullulanase and methods of saccharification of starch using pullulanase
    7.
    发明授权
    Pullulanase, methods of producing pullulanase and methods of saccharification of starch using pullulanase 失效
    支链淀粉酶，支链淀粉酶的生产方法和使用支链淀粉酶的淀粉糖化方法

    公开(公告)号：US5316924A

    公开(公告)日：1994-05-31

    申请号：US890518

    申请日：1992-05-28

    申请人： Yoshiyuki Takasaki

    发明人： Yoshiyuki Takasaki

    IPC分类号： C12N9/44 ,  C12P19/14 ,  C12P19/16 ,  C12R1/01 ,  C12P21/00

    CPC分类号： C12R1/01 ,  C12N9/2457 ,  C12P19/16 ,  C12Y302/01041

    摘要： The present invention provides a novel pullulanase, methods of producing and isolating the pullulanase and methods of saccharifying starch using the pullulanase. The pullulanase of the invention works in a wide pH range, as large as pH 4-9. In addition, the pullulanase is heat stable and its optimum temperature is about 60.degree. C. Therefore, the pullulanase of the invention can be used in the saccharification of starch along with amylase, which is enzymatically active at pH 4-9, to increase the yield of saccharides.

    摘要翻译： 本发明提供了一种新型支链淀粉酶，生产和分离支链淀粉酶的方法和使用支链淀粉酶糖化淀粉的方法。 本发明的支链淀粉酶在pH 4-9的广泛pH范围内起作用。 此外，支链淀粉酶是热稳定的，其最适温度为约60℃。因此，本发明的支链淀粉酶可用于淀粉酶的糖化以及在pH 4-9下酶促活性的淀粉酶，以增加 产糖量。

    Process for mannose and mannose isomerase production using mannose isomerase-producing pseudomonas
    9.
    发明授权
    Process for mannose and mannose isomerase production using mannose isomerase-producing pseudomonas 失效
    使用产甘露糖异构酶的假单胞菌生产甘露糖和甘露糖异构酶的方法

    公开(公告)号：US5240717A

    公开(公告)日：1993-08-31

    申请号：US851665

    申请日：1992-03-16

    申请人： Yoshiyuki Takasaki ,  Takaichi Ohya

    发明人： Yoshiyuki Takasaki ,  Takaichi Ohya

    IPC分类号： C12N9/90

    CPC分类号： C12R1/38 ,  C12N9/90 ,  Y10S435/874

    摘要： A mannose isomerase having excellent properties for industrial use, such as high thermal stability and resistance to high substrate concentrations, can be produced by culturing a strain of Pseudomonas (sp. AM-9582), and extracting it from the cells of AM-9582. Mannose can be effectively produced from fructose of high concentrations using the enzyme.

    摘要翻译： 可以通过培养假单胞菌（SP.AM-9582）菌株并从AM-9582的细胞中提取具有优异工业性质的甘露糖异构酶，例如高热稳定性和高底物浓度。 使用酶可以从高浓度的果糖有效地制备甘露糖。