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公开(公告)号：US07579151B2
公开(公告)日：2009-08-25
申请号：US11191221
申请日：2005-07-27
申请人： Young-sun Lee , Jung-im Han , Hyo-yeon Lee , Kak Namkoong , Kwang-wook Oh
发明人： Young-sun Lee , Jung-im Han , Hyo-yeon Lee , Kak Namkoong , Kwang-wook Oh
CPC分类号： C12N15/1006
摘要： Provided is a method of isolating and purifying biomolecules using a hydrogel, the method including: bring a sample containing charged biomolecules into contact with a hydrogel to bind the biomolecules to the hydrogel; washing the hydrogel bound with the biomolecules; and eluting the bound biomolecules using an elution solvent. According to the method, the use of a hydrogel with a large surface area reduces the isolation time of biomolecules to 5 min or less, an external device such as an electromagnet is not required, and small-sized systems or LOC can be easily implemented due to applicability to microsystems through a polymer patterning technique.
摘要翻译： 提供了使用水凝胶分离和纯化生物分子的方法，该方法包括：使含有带电生物分子的样品与水凝胶接触以将生物分子结合至水凝胶; 洗涤与生物分子结合的水凝胶; 并使用洗脱溶剂洗脱结合的生物分子。 根据该方法，使用具有大表面积的水凝胶将生物分子的隔离时间减少到5分钟以下，不需要诸如电磁体的外部装置，并且可以容易地实现小尺寸系统或LOC 通过聚合物图案化技术适用于微系统。
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公开(公告)号：US20060127887A1
公开(公告)日：2006-06-15
申请号：US11191221
申请日：2005-07-27
申请人： Young-sun Lee , Jung-im Han , Hyo-yeon Lee , Kak Namkoong , Kwang-wook Oh
发明人： Young-sun Lee , Jung-im Han , Hyo-yeon Lee , Kak Namkoong , Kwang-wook Oh
CPC分类号： C12N15/1006
摘要： Provided is a method of isolating and purifying biomolecules using a hydrogel, the method including: bring a sample containing charged biomolecules into contact with a hydrogel to bind the biomolecules to the hydrogel; washing the hydrogel bound with the biomolecules; and eluting the bound biomolecules using an elution solvent. According to the method, the use of a hydrogel with a large surface area reduces the isolation time of biomolecules to 5 min or less, an external device such as an electromagnet is not required, and small-sized systems or LOC can be easily implemented due to applicability to microsystems through a polymer patterning technique.
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公开(公告)号：US20080090275A1
公开(公告)日：2008-04-17
申请号：US11783351
申请日：2007-04-09
申请人： Yoon-kyoung Cho , Jeong-gun Lee , Jong-myeon Park , Young-sun Lee , Hyo-yeon Lee
发明人： Yoon-kyoung Cho , Jeong-gun Lee , Jong-myeon Park , Young-sun Lee , Hyo-yeon Lee
IPC分类号： C12N13/00
CPC分类号： C12N1/066 , C12M47/06 , C12N13/00 , G01N33/54326
摘要： Provided is a method for concentrating and disrupting cells or viruses, the method employs magnetic particles which are capable of binding the cells or viruses to capture the cells or viruses and a laser to disrupt the cells or viruses. Since the same particles are used in target cell separation and cell lysis, there is no need to add additional particles in a laser lysis process, and thus integration of a target cell separation, concentration, purification, and nucleic acid extraction process is easy.
摘要翻译： 提供了一种用于浓缩和破坏细胞或病毒的方法，该方法使用能够结合细胞或病毒以捕获细胞或病毒的磁性颗粒和激光以破坏细胞或病毒。 由于在靶细胞分离和细胞裂解中使用相同的颗粒，因此不需要在激光裂解过程中添加额外的颗粒，因此靶细胞分离，浓缩，纯化和核酸提取过程的整合是容易的。
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