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    Method of concentrating and disrupting cells or viruses
    1.
    发明申请
    Method of concentrating and disrupting cells or viruses 审中-公开
    浓缩和破坏细胞或病毒的方法

    公开(公告)号：US20080090275A1

    公开(公告)日：2008-04-17

    申请号：US11783351

    申请日：2007-04-09

    申请人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Jong-myeon Park ,  Young-sun Lee ,  Hyo-yeon Lee

    发明人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Jong-myeon Park ,  Young-sun Lee ,  Hyo-yeon Lee

    IPC分类号： C12N13/00

    CPC分类号： C12N1/066 ,  C12M47/06 ,  C12N13/00 ,  G01N33/54326

    摘要： Provided is a method for concentrating and disrupting cells or viruses, the method employs magnetic particles which are capable of binding the cells or viruses to capture the cells or viruses and a laser to disrupt the cells or viruses. Since the same particles are used in target cell separation and cell lysis, there is no need to add additional particles in a laser lysis process, and thus integration of a target cell separation, concentration, purification, and nucleic acid extraction process is easy.

    摘要翻译： 提供了一种用于浓缩和破坏细胞或病毒的方法，该方法使用能够结合细胞或病毒以捕获细胞或病毒的磁性颗粒和激光以破坏细胞或病毒。 由于在靶细胞分离和细胞裂解中使用相同的颗粒，因此不需要在激光裂解过程中添加额外的颗粒，因此靶细胞分离，浓缩，纯化和核酸提取过程的整合是容易的。

    CENTRIFUGAL FORCE BASED MICROFLUIDIC DEVICE FOR DILUTION AND MICROFLUIDIC SYSTEM INCLUDING THE SAME
    2.
    发明申请
    CENTRIFUGAL FORCE BASED MICROFLUIDIC DEVICE FOR DILUTION AND MICROFLUIDIC SYSTEM INCLUDING THE SAME 失效
    基于离散力的微流体装置，包括它们的渗透和微流体系统

    公开(公告)号：US20080193336A1

    公开(公告)日：2008-08-14

    申请号：US12029480

    申请日：2008-02-12

    申请人： Yoon-kyoung CHO ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park ,  Young-sun Lee

    发明人： Yoon-kyoung CHO ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park ,  Young-sun Lee

    IPC分类号： B01L11/00

    CPC分类号： B01L3/502738 ,  B01L3/50273 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0806 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0677

    摘要： Provided are a centrifugal force based microfluidic device which can automatically perform a dilution operation and a microfluidic system including the same. The centrifugal force based microfluidic device for dilution includes a rotatable disk type platform, a mixing chamber disposed on the platform; a buffer solution storage disposed on a portion of the platform which is closer to a center of the platform than the mixing chamber, connected to the mixing chamber through a channel to supply a predetermined amount of buffer solution to the mixing chamber at least one time, and a plurality of diluted solution chambers which are disposed on a portion of the platform which is farther from the center of the platform than the mixing chamber, are each connected to the mixing chamber through flow paths extended from a middle exit corresponding to a predetermined water level, and sequentially receiving a solution which is serially diluted in the mixing chamber at least one time.

    摘要翻译： 提供了一种能够自动执行稀释操作的离心力微流体装置和包括其的微流体系统。 用于稀释的基于离心力的微流体装置包括可旋转盘式平台，设置在平台上的混合室; 缓冲溶液储存器，其设置在所述平台的比混合室更靠近所述平台的中心的部分上，所述缓冲溶液存储器经由通道至少一次向所述混合室提供预定量的缓冲溶液，所述通道连接到所述混合室， 并且多个稀释溶液室通过从对应于预定水的中间出口延伸的流路连接到平台的比混合室更远离平台中心的部分上的稀释溶液室 至少一次连续地接收在混合室中连续稀释的溶液。

    Centrifugal force based microfluidic device for dilution and microfluidic system including the same
    3.
    发明授权
    Centrifugal force based microfluidic device for dilution and microfluidic system including the same 失效
    用于稀释和微流体系统的基于离心力的微流体装置包括相同的

    公开(公告)号：US07951332B2

    公开(公告)日：2011-05-31

    申请号：US12029480

    申请日：2008-02-12

    申请人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park ,  Young-sun Lee

    发明人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park ,  Young-sun Lee

    IPC分类号： B01L11/00

    CPC分类号： B01L3/502738 ,  B01L3/50273 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0806 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0677

    摘要： Provided are a centrifugal force based microfluidic device which can automatically perform a dilution operation and a microfluidic system including the same. The centrifugal force based microfluidic device for dilution includes a rotatable disk type platform, a mixing chamber disposed on the platform; a buffer solution storage disposed on a portion of the platform which is closer to a center of the platform than the mixing chamber, connected to the mixing chamber through a channel to supply a predetermined amount of buffer solution to the mixing chamber at least one time, and a plurality of diluted solution chambers which are disposed on a portion of the platform which is farther from the center of the platform than the mixing chamber, are each connected to the mixing chamber through flow paths extended from a middle exit corresponding to a predetermined water level, and sequentially receiving a solution which is serially diluted in the mixing chamber at least one time.

    摘要翻译： 提供了一种能够自动执行稀释操作的离心力微流体装置和包括其的微流体系统。 用于稀释的基于离心力的微流体装置包括可旋转盘式平台，设置在平台上的混合室; 缓冲溶液储存器，其设置在所述平台的比混合室更靠近所述平台的中心的部分上，所述缓冲溶液存储器经由通道至少一次向所述混合室提供预定量的缓冲溶液，所述通道连接到所述混合室， 并且多个稀释溶液室通过从对应于预定水的中间出口延伸的流路连接到平台的比混合室更远离平台中心的部分上的稀释溶液室 至少一次连续地接收在混合室中连续稀释的溶液。

    Centrifugal force-based microfluidic device for protein detection and microfluidic system including the same
    5.
    发明授权
    Centrifugal force-based microfluidic device for protein detection and microfluidic system including the same 有权
    用于蛋白质检测和微流体系统的基于离心力的微流体装置包括相同的

    公开(公告)号：US07951333B2

    公开(公告)日：2011-05-31

    申请号：US12851819

    申请日：2010-08-06

    申请人： Beom Seok Lee ,  Yoon Kyoung Cho ,  Jeong Gun Lee ,  Jong-myeon Park

    发明人： Beom Seok Lee ,  Yoon Kyoung Cho ,  Jeong Gun Lee ,  Jong-myeon Park

    IPC分类号： G01N9/30

    CPC分类号： B01L3/502738 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0806 ,  B01L2300/0867 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0442 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  G01N33/54326 ,  G01N33/54366 ,  Y10T436/25375

    摘要： A centrifugal force-based microfluidic device for the detection of a target biomolecule and a microfluidic system including the same are provided. The device includes a body of revolution; a microfluidic structure disposed in the body of revolution including chambers, channels connecting the chambers, and valves disposed in the channels to control fluid flow, the microfluidic structures transmitting fluid using centrifugal force due to rotation of the body of revolution; and beads disposed in the microfluidic structures, the beads having capture probes on the surfaces thereof which are selectively bonded with target protein; and a detection probe disposed in the microfluidic structures and selectively bonded to the target protein, and which includes a material required to express an optical signal, wherein the microfluidic structure mixes the beads, biological samples, and the detection probe to react and washes and separates the beads after the reaction.

    摘要翻译： 提供了用于检测靶生物分子的离心力微流体装置和包括其的微流体系统。 该装置包括一个革命体; 设置在旋转体中的微流体结构，包括腔室，连接腔室的通道，以及设置在通道中的阀，以控制流体流动，微流体结构由于旋转体旋转而使用离心力传送流体; 和珠子，其设置在微流体结构中，珠粒在其表面上具有与靶蛋白质选择性结合的捕获探针; 以及设置在微流体结构中并选择性地结合到靶蛋白上并且包括表达光信号所需的材料的检测探针，其中微流体结构混合珠，生物样品和检测探针以反应和洗涤和分离 反应后的珠子。

    Centrifugal force-based microfluidic device for nucleic acid extraction and microfluidic system including the microfluidic device
    6.
    发明授权
    Centrifugal force-based microfluidic device for nucleic acid extraction and microfluidic system including the microfluidic device 有权
    用于核酸提取的离心力微流体装置和包括微流体装置的微流体系统

    公开(公告)号：US08420026B2

    公开(公告)日：2013-04-16

    申请号：US13594293

    申请日：2012-08-24

    申请人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park

    发明人： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park

    IPC分类号： B04B13/00

    CPC分类号： B01L3/502738 ,  B01F13/0059 ,  B01F15/0201 ,  B01F15/0233 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2300/0806 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/004 ,  F16K2099/0076 ,  F16K2099/0084

    摘要： A centrifugal force-based microfluidic device for nucleic acid extraction and a microfluidic system are provided. The microfluidic device includes a body of revolution; a microfluidic structure disposed in the body of revolution, the microfluidic structure including a plurality of chambers, channels connecting the chambers, and valves disposed in the channels to control fluid flow, the microfluidic structure transmitting the fluid using centrifugal force due to rotation of the body of revolution; and magnetic beads contained in one of the chambers which collect a target material from a biomaterial sample flowing into the chamber, wherein the microfluidic structure washes the magnetic beads which collect the target material, and separates nucleic acid by electromagnetic wave irradiation from an external energy source to the magnetic beads. The microfluidic system includes the microfluidic device; a rotation operating unit which rotates the body of revolution; and an external energy source which irradiates electromagnetic waves.

    摘要翻译： 提供了用于核酸提取的离心力微流体装置和微流体系统。 微流体装置包括一个革命体; 设置在旋转体内的微流体结构，所述微流体结构包括多个室，连接所述室的通道和设置在通道中的阀以控制流体流动，所述微流体结构由于所述体的旋转而使用离心力传递流体 的革命 以及包含在其中一个室中的磁珠，其从流入腔室的生物材料样品收集目标材料，其中微流体结构洗涤收集目标材料的磁珠，并通过来自外部能量源的电磁波照射分离核酸 到磁珠。 微流体系统包括微流体装置; 使旋转体旋转的旋转操作单元; 以及照射电磁波的外部能量源。

    MICROFLUIDIC DEVICE USING MICROFLUIDIC CHIP AND MICROFLUIDIC DEVICE USING BIOMOLECULE MICROARRAY CHIP
    8.
    发明申请
    MICROFLUIDIC DEVICE USING MICROFLUIDIC CHIP AND MICROFLUIDIC DEVICE USING BIOMOLECULE MICROARRAY CHIP 有权
    使用生物分子微量芯片的微流控芯片和微流化器件的微流体装置

    公开(公告)号：US20110257037A1

    公开(公告)日：2011-10-20

    申请号：US13168298

    申请日：2011-06-24

    申请人： Beom-seok LEE ,  Jeong-gun Lee ,  Jong-myeon Park ,  Yoon-kyoung Cho

    发明人： Beom-seok LEE ,  Jeong-gun Lee ,  Jong-myeon Park ,  Yoon-kyoung Cho

    IPC分类号： C40B40/00 ,  C40B40/10 ,  C40B40/02 ,  C40B40/06

    CPC分类号： B01L3/502738 ,  B01L3/502707 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/0621 ,  B01L2200/16 ,  B01L2300/04 ,  B01L2300/0636 ,  B01L2300/0806 ,  B01L2400/0409 ,  B01L2400/0677 ,  Y10T436/15

    摘要： Disclosed is a microfluidic device including a microfluidic structure formed in a platform in which various examinations, such as an immune serum examination, can be automatically performed using the biomolecule microarray chip. The biomolecule microarray chip-type microfluidic device using a biomolecule microarray chip comprises: a platform which is rotatable; a microfluidic structure disposed in the platform, comprising: a plurality of chambers; a plurality of channels connecting the chambers each other; and a plurality of valves controlling flow of fluids through the channels, wherein the microfluidic structure controls flow of a fluid sample using rotation of the platform and the valves; and a biomolecule microarray chip mounted in the platform such that biomolecule capture probes bound to the biomolecule microarray chip contact the fluid sample in the microfluidic structure.

    摘要翻译： 公开了一种微流体装置，其包括在平台中形成的微流体结构，其中可以使用生物分子微阵列芯片自动执行各种检查，例如免疫血清检查。 使用生物分子微阵列芯片的生物分子微阵列芯片型微流器件包括：可旋转的平台; 设置在平台中的微流体结构，包括：多个室; 连接所述室的多个通道; 以及多个阀，其控制通过所述通道的流体流动，其中所述微流体结构通过所述平台和所述阀的旋转来控制流体样品的流动; 以及安装在平台中的生物分子微阵列芯片，使得与生物分子微阵列芯片结合的生物分子捕获探针与微流体结构中的流体样品接触。

    Microfluidic valve, method of manufacturing the same, and microfluidic device comprising the microfluidic valve
    9.
    发明授权
    Microfluidic valve, method of manufacturing the same, and microfluidic device comprising the microfluidic valve 有权
    微流控阀，其制造方法以及包含微流体阀的微流体装置

    公开(公告)号：US07980272B2

    公开(公告)日：2011-07-19

    申请号：US12133396

    申请日：2008-06-05

    申请人： Jong-myeon Park ,  Yoon-kyoung Cho ,  Beom-seok Lee ,  Jeong-gun Lee

    发明人： Jong-myeon Park ,  Yoon-kyoung Cho ,  Beom-seok Lee ,  Jeong-gun Lee

    IPC分类号： F15C1/04

    CPC分类号： F16K99/0001 ,  F16K99/0032 ,  F16K99/004 ,  F16K2099/0084 ,  Y10T29/49412 ,  Y10T137/2191 ,  Y10T137/2196

    摘要： Provided is a microfluidic valve, a method of manufacturing the microfluidic valve, and a microfluidic device that employs the microfluidic valve. The microfluidic valve includes a platform that includes two substrates combined facing each other; a channel having a first depth allowing a fluid to flow between the two substrates; a valve gap that is disposed on at least a region of the channel and has a second depth which is smaller than the first depth; and a valve plug that is disposed to fill the valve gap and is formed of a valve material made by mixing a phase change material, which is solid at room temperature, with a plurality of exothermic particles that emit an amount of heat sufficient to melt the phase change material by absorbing electromagnetic waves.

    摘要翻译： 本发明提供一种微流体阀，微流体阀的制造方法和采用该微流体阀的微流体装置。 该微流体阀包括一个平台，该平台包括彼此相结合的两个基板; 具有允许流体在两个基板之间流动的第一深度的通道; 阀间隙，其设置在所述通道的至少一个区域上，并且具有小于所述第一深度的第二深度; 以及阀塞，其设置成填充阀间隙，并且由阀室材料形成，所述阀门材料是通过将在室温下为固体的相变材料与多个放出足以熔化的 相变材料吸收电磁波。