公开(公告)号：US20090053801A1

公开(公告)日：2009-02-26

申请号：US12288373

申请日：2008-10-20

申请人： Yubo Miao ,  Yu Chen ,  Tit Meng Lim ,  Chew Kiat Heng

发明人： Yubo Miao ,  Yu Chen ,  Tit Meng Lim ,  Chew Kiat Heng

IPC分类号： C12M1/40

CPC分类号： B29C51/082 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/1805 ,  B29C33/40 ,  B29K2023/12 ,  B29K2033/12 ,  B29K2067/00 ,  B29K2069/00 ,  B29K2883/00 ,  B29L2031/756 ,  Y10S435/809 ,  Y10S436/809 ,  Y10T436/25

摘要： Disposable units in current use for performing PCR are limited by their heat block ramping rates and by the thermal diffusion delay time through the plastic wall as well as by the sample itself. This limitation has been overcome by forming a disposable plastic chip using a simple deformation process wherein one or more plastic sheets are caused, through hydrostatic pressure, to conform to the surface of a suitable mold. After a given disposable chip has been filled with liquid samples, it is brought into close contact with an array of heating blocks that seals each sample within its own chamber, allowing each sample to then be heat treated as desired.

摘要翻译： 当前用于进行PCR的一次性单元受到其热阻斜率和通过塑料壁以及样品本身的热扩散延迟时间的限制。 通过使用简单的变形过程形成一次性塑料芯片已经克服了这种限制，其中通过静水压力使一个或多个塑料片材与合适的模具的表面一致。 在给定的一次性芯片已经充满液体样品之后，它与一个加热块阵列紧密接触，该加热块在其自己的室内密封每个样品，然后根据需要对每个样品进行热处理。

公开(公告)号：US07442542B2

公开(公告)日：2008-10-28

申请号：US10613599

申请日：2003-07-03

申请人： Yubo Miao ,  Yu Chen ,  Tit Meng Lim ,  Chew Kiat Heng

发明人： Yubo Miao ,  Yu Chen ,  Tit Meng Lim ,  Chew Kiat Heng

IPC分类号： C12M1/02 ,  C12M1/40 ,  C12P19/34 ,  B28B7/30 ,  B29C43/56 ,  B29C51/14 ,  G01N1/44

CPC分类号： B29C51/082 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/1805 ,  B29C33/40 ,  B29K2023/12 ,  B29K2033/12 ,  B29K2067/00 ,  B29K2069/00 ,  B29K2883/00 ,  B29L2031/756 ,  Y10S435/809 ,  Y10S436/809 ,  Y10T436/25

摘要： Disposable units in current use for performing PCR are limited by their heat block ramping rates and by the thermal diffusion delay time through the plastic wall as well as by the sample itself. This limitation has been overcome by forming a disposable plastic chip using a simple deformation process wherein one or more plastic sheets are caused, through hydrostatic pressure, to conform to the surface of a suitable mold. After a given disposable chip has been filled with liquid samples, it is brought into close contact with an array of heating blocks that seals each sample within its own chamber, allowing each sample to then be heat treated as desired.

摘要翻译： 当前用于进行PCR的一次性单元受到其热阻斜率和通过塑料壁以及样品本身的热扩散延迟时间的限制。 通过使用简单的变形过程形成一次性塑料芯片已经克服了这种限制，其中通过静水压力使一个或多个塑料片材与合适的模具的表面一致。 在给定的一次性芯片已经充满液体样品之后，它与一个加热块阵列紧密接触，该加热块在其自己的室内密封每个样品，然后根据需要对每个样品进行热处理。

公开(公告)号：US08080411B2

公开(公告)日：2011-12-20

申请号：US12288373

申请日：2008-10-20

申请人： Yubo Miao ,  Yu Chen ,  Tit Meng Lim ,  Chew Kiat Heng

发明人： Yubo Miao ,  Yu Chen ,  Tit Meng Lim ,  Chew Kiat Heng

IPC分类号： C12M1/02 ,  C12M1/40 ,  G01N1/44

CPC分类号： B29C51/082 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/1805 ,  B29C33/40 ,  B29K2023/12 ,  B29K2033/12 ,  B29K2067/00 ,  B29K2069/00 ,  B29K2883/00 ,  B29L2031/756 ,  Y10S435/809 ,  Y10S436/809 ,  Y10T436/25

摘要： Disposable units in current use for performing PCR are limited by their heat block ramping rates and by the thermal diffusion delay time through the plastic wall as well as by the sample itself. This limitation has been overcome by forming a disposable plastic chip using a simple deformation process wherein one or more plastic sheets are caused, through hydrostatic pressure, to conform to the surface of a suitable mold. After a given disposable chip has been filled with liquid samples, it is brought into close contact with an array of heating blocks that seals each sample within its own chamber, allowing each sample to then be heat treated as desired.

摘要翻译： 当前用于进行PCR的一次性单元受到其热阻斜率和通过塑料壁以及样品本身的热扩散延迟时间的限制。 通过使用简单的变形过程形成一次性塑料芯片已经克服了这种限制，其中通过静水压力使一个或多个塑料片材与合适的模具的表面一致。 在给定的一次性芯片已经充满液体样品之后，它与一个加热块阵列紧密接触，该加热块在其自己的室内密封每个样品，然后根据需要对每个样品进行热处理。

公开(公告)号：US06716661B2

公开(公告)日：2004-04-06

申请号：US10147006

申请日：2002-05-16

申请人： Quanbo Zou ,  Yu Chen ,  Janak Singh ,  Tit Meng Lim ,  Tie Yan ,  Chew Kiat Heng

发明人： Quanbo Zou ,  Yu Chen ,  Janak Singh ,  Tit Meng Lim ,  Tie Yan ,  Chew Kiat Heng

IPC分类号： H01L2100

CPC分类号： B81C1/00214 ,  B81C2201/0132 ,  F04B43/043

摘要： Formation of micro-fluidic systems is normally achieved using a multi-wafer fabrication procedure. The present invention teaches how a complete micro-fluidic system can be implemented on a single chip. The invention uses only dry etch processes to form micro-chambers. In particular, it makes use of deep reactive ion etching whereby multiple trenches of differing depths may be formed simultaneously. Buried micro-chambers are formed by isotropically increasing trench widths using an etchant that does not attack the mask so the trenches grow wider beneath the surface until they merge. Deposition of a dielectric layer over the trenches allows some trenches to be sealed and some to be left open. Micro-pumps are formed by including in the micro-chamber roof a layer that is used to change chamber volume either through electrostatically induced motion or through thermal mismatch as a result of its being heated.

摘要翻译： 微流体系统的形成通常使用多晶片制造程序来实现。 本发明教导了如何在单个芯片上实现完整的微流体系统。 本发明仅使用干蚀刻工艺来形成微室。 特别地，它利用深反应离子蚀刻，从而可以同时形成不同深度的多个沟槽。 通过使用不侵蚀掩模的蚀刻剂各向同性地增加沟槽宽度来形成掩埋的微室，使得沟槽在表面下方变得更宽，直到它们合并。 电介质层沉积在沟槽上方允许一些沟槽被密封，有些则被打开。 通过在微室顶中包含用于通过静电诱导运动或通过其被加热的结果的热失配来改变室容积的层而形成微泵。