公开(公告)号：US20110114485A1

公开(公告)日：2011-05-19

申请号：US13054271

申请日：2009-07-15

申请人： Koji Sakairi ,  Satonari Akutsu ,  Yuji Maruo ,  Takateru Matsunaga ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Nao Sakaguchi ,  Kenji Yokoyama

发明人： Koji Sakairi ,  Satonari Akutsu ,  Yuji Maruo ,  Takateru Matsunaga ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Nao Sakaguchi ,  Kenji Yokoyama

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N27/44773

摘要： An electrophoresis apparatus includes a sample separating section for containing a sample separating medium for separating a sample in a horizontal direction, the sample separating section containing the sample separating medium in such a manner that the sample separating medium has an exposed portion at least one end of a surface of the sample separating medium, the surface being in parallel with the horizontal direction; and medium connecting means for connecting a sample containing medium to the sample separating medium at a connecting region, the sample containing medium containing a sample, and the connecting region satisfying a particular equation. By this, an electrophoresis technique with better accuracy than conventional arts can be provided.

摘要翻译： 电泳装置包括样品分离部分，用于容纳用于在水平方向上分离样品的样品分离介质，样品分离部分包含样品分离介质，使得样品分离介质具有暴露部分，至少一端 所述样品分离介质的表面与所述水平方向平行; 以及介质连接装置，用于在连接区域连接包含样品的介质到样品分离介质，所述样品包含含有样品的介质和满足特定等式的连接区域。 由此，可以提供比现有技术更好的精度的电泳技术。

公开(公告)号：US20070045118A1

公开(公告)日：2007-03-01

申请号：US11510838

申请日：2006-08-28

申请人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

发明人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

IPC分类号： G01N27/00

CPC分类号： G01N27/44778

摘要： The present invention is to provide a sample separation instrument used in a sample separation apparatus which includes: holding means for holding a first medium supporter which supports a first medium; and driving means for moving fixing means or the holding means in a direction parallel or perpendicular to a plane whose sides extend in the first direction and in the second directions. The sample separation instrument includes an insulator for storing a second medium which allows a sample separated in the first medium in the first direction to be further separated in the second direction different from the first direction, wherein: the insulator includes a first opening and a second opening each of which defines the second direction in which the second medium is electrified, and the second opening has a shape which allows the first medium containing the separated sample to be attached to the second medium along the first direction. The present invention realizes automation of the two-dimensional electrophoresis to enhance the convenience of the two-dimensional electrophoresis, to less depend on the operator's skill, and to enhance the reproducibility of the electrophoresis result.

摘要翻译： 本发明提供一种在样品分离装置中使用的样品分离装置，其包括：保持装置，用于保持支撑第一介质的第一介质支持体; 以及用于在平行或垂直于其侧面沿第一方向和第二方向延伸的平面的方向上移动固定装置或保持装置的驱动装置。 样品分离装置包括用于存储第二介质的绝缘体，其允许在第一方向上在第一介质中分离的样品在与第一方向不同的第二方向上进一步分离，其中：绝缘体包括第一开口和第二开口 其中每个开口限定第二介质带电的第二方向，第二开口具有允许包含分离样品的第一介质沿着第一方向附着到第二介质的形状。 本发明实现了二维电泳的自动化，以增强二维电泳的便利性，较少依赖于操作者的技能，并提高电泳结果的再现性。

公开(公告)号：US08500981B2

公开(公告)日：2013-08-06

申请号：US13054271

申请日：2009-07-15

申请人： Koji Sakairi ,  Satonari Akutsu ,  Yuji Maruo ,  Takateru Matsunaga ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Nao Sakaguchi ,  Kenji Yokoyama

发明人： Koji Sakairi ,  Satonari Akutsu ,  Yuji Maruo ,  Takateru Matsunaga ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Nao Sakaguchi ,  Kenji Yokoyama

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N27/44773

摘要： An electrophoresis apparatus includes a sample separating section for containing a sample separating medium for separating a sample in a horizontal direction, the sample separating section containing the sample separating medium in such a manner that the sample separating medium has an exposed portion at least one end of a surface of the sample separating medium, the surface being in parallel with the horizontal direction; and medium connecting means for connecting a sample containing medium to the sample separating medium at a connecting region, the sample containing medium containing a sample, and the connecting region satisfying a particular equation. By this, an electrophoresis technique with better accuracy than conventional arts can be provided.

摘要翻译： 电泳装置包括样品分离部分，用于容纳用于在水平方向上分离样品的样品分离介质，样品分离部分包含样品分离介质，使得样品分离介质具有暴露部分，至少一端 所述样品分离介质的表面与所述水平方向平行; 以及介质连接装置，用于在连接区域连接包含样品的介质到样品分离介质，所述样品包含含有样品的介质和满足特定等式的连接区域。 由此，可以提供比现有技术更好的精度的电泳技术。

公开(公告)号：US20080296158A1

公开(公告)日：2008-12-04

申请号：US12130691

申请日：2008-05-30

申请人： Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama ,  Koji Sakairi ,  Satonari Akutsu

发明人： Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama ,  Koji Sakairi ,  Satonari Akutsu

IPC分类号： C25B7/00 ,  C25B9/00

CPC分类号： G01N27/44704 ,  B01L3/50273

摘要： A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.

摘要翻译： 用于电泳的装置将电压施加到与多个电导体接触的介质，使得相邻导体的电位在一定范围内。 这样可以防止电泳速度下降。 用于电泳和转印的装置包括具有彼此绝缘并沿特定方向布置的多个电极区域的电极。 这允许提供用于电泳和转移的实用且易于使用的装置。 用于传送的装置将施加的电压或施加的电压持续时间改变到某个位置到另一位置。 这样可以提高传输效率。

公开(公告)号：US08702950B2

公开(公告)日：2014-04-22

申请号：US13607844

申请日：2012-09-10

申请人： Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama ,  Koji Sakairi ,  Satonari Akutsu

发明人： Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama ,  Koji Sakairi ,  Satonari Akutsu

IPC分类号： G01N27/26 ,  B01D57/02

CPC分类号： G01N27/44704 ,  B01L3/50273

摘要： A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.

摘要翻译： 用于电泳的装置将电压施加到与多个电导体接触的介质，使得相邻导体的电位在一定范围内。 这样可以防止电泳速度下降。 用于电泳和转印的装置包括具有彼此绝缘并沿特定方向布置的多个电极区域的电极。 这允许提供用于电泳和转移的实用且易于使用的装置。 用于传送的装置将施加的电压或施加的电压持续时间改变到某个位置到另一位置。 这样可以提高传输效率。

公开(公告)号：US20130015069A1

公开(公告)日：2013-01-17

申请号：US13607844

申请日：2012-09-10

申请人： Yuji MARUO ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama ,  Koji Sakairi ,  Satonari Akutsu

发明人： Yuji MARUO ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Kenji Yokoyama ,  Koji Sakairi ,  Satonari Akutsu

IPC分类号： B01D57/02

CPC分类号： G01N27/44704 ,  B01L3/50273

摘要： A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.

摘要翻译： 用于电泳的装置将电压施加到与多个电导体接触的介质，使得相邻导体的电位在一定范围内。 这样可以防止电泳速度下降。 用于电泳和转印的装置包括具有彼此绝缘并沿特定方向布置的多个电极区域的电极。 这允许提供用于电泳和转移的实用且易于使用的装置。 用于传送的装置将施加的电压或施加的电压持续时间改变到某个位置到另一位置。 这样可以提高传输效率。

公开(公告)号：US07964077B2

公开(公告)日：2011-06-21

申请号：US11510838

申请日：2006-08-28

申请人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

发明人： Yuji Maruo ,  Katsuyoshi Takahashi ,  Yutaka Unuma ,  Keisuke Usui ,  Hideki Kinoshita ,  Kisho Shiseki ,  Yoshio Suzuki ,  Atsunori Hiratsuka ,  Hiroyuki Fukui ,  Kenji Yokoyama ,  Ichiji Namatame ,  Kouhei Yodoya ,  Yasuhiro Ogawa ,  Koji Sakairi ,  Chie Hayashida ,  Satonari Akutsu ,  Kazuyoshi Yano ,  Isao Karube

IPC分类号： G01N27/26 ,  G01N27/447

CPC分类号： G01N27/44778

摘要： The present invention is to provide a sample separation instrument used in a sample separation apparatus which includes: holding means for holding a first medium supporter which supports a first medium; and driving means for moving fixing means or the holding means in a direction parallel or perpendicular to a plane whose sides extend in the first direction and in the second directions. The sample separation instrument includes an insulator for storing a second medium which allows a sample separated in the first medium in the first direction to be further separated in the second direction different from the first direction, wherein: the insulator includes a first opening and a second opening each of which defines the second direction in which the second medium is electrified, and the second opening has a shape which allows the first medium containing the separated sample to be attached to the second medium along the first direction. The present invention realizes automation of the two-dimensional electrophoresis to enhance the convenience of the two-dimensional electrophoresis, to less depend on the operator's skill, and to enhance the reproducibility of the electrophoresis result.

摘要翻译： 本发明提供一种在样品分离装置中使用的样品分离装置，其包括：保持装置，用于保持支撑第一介质的第一介质支持体; 以及用于在平行或垂直于其侧面沿第一方向和第二方向延伸的平面的方向上移动固定装置或保持装置的驱动装置。 样品分离装置包括用于存储第二介质的绝缘体，其允许在第一方向上在第一介质中分离的样品在与第一方向不同的第二方向上进一步分离，其中：绝缘体包括第一开口和第二开口 其中每个开口限定第二介质带电的第二方向，第二开口具有允许包含分离样品的第一介质沿着第一方向附着到第二介质的形状。 本发明实现了二维电泳的自动化，以增强二维电泳的便利性，较少依赖于操作者的技能，并提高电泳结果的再现性。

公开(公告)号：US20120273360A1

公开(公告)日：2012-11-01

申请号：US13518686

申请日：2010-12-21

申请人： Hideki Kinoshita ,  Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Koji Sakairi ,  Satonari Akutsu

发明人： Hideki Kinoshita ,  Yuji Maruo ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Koji Sakairi ,  Satonari Akutsu

IPC分类号： C25B9/00 ,  G01N27/447

CPC分类号： G01N27/44756 ,  G01N27/44743

摘要： An instrument for electrophoresis includes a sample-separating portion in which a slab sample-separating medium that separates a sample is provided. The instrument for electrophoresis includes a sample-transporting portion that transports a sample-containing medium containing the sample to the sample-separating portion. The instrument for electrophoresis includes a positioning portion that is provided to the sample-separating portion and/or the sample-transporting portion and used for connecting the sample-transporting portion to a predetermined position of the sample-separating portion.

摘要翻译： 电子仪器包括样品分离部分，其中提供分离样品的板坯样品分离介质。 用于电泳的仪器包括将含有样品的含样本培养基输送到样品分离部分的样品输送部分。 电泳仪包括设置在样品分离部分和/或样品输送部分上的定位部分，用于将样品输送部分连接到样品分离部分的预定位置。

公开(公告)号：US08419916B2

公开(公告)日：2013-04-16

申请号：US13073835

申请日：2011-03-28

申请人： Koji Sakairi ,  Satonari Akutsu ,  Atsunori Hiratsuka ,  Kenji Yokoyama ,  Yuji Maruo ,  Hiroshi Ohki

发明人： Koji Sakairi ,  Satonari Akutsu ,  Atsunori Hiratsuka ,  Kenji Yokoyama ,  Yuji Maruo ,  Hiroshi Ohki

IPC分类号： G01N27/26

CPC分类号： G01N27/44769

摘要： A biological molecule separation apparatus for separating a biological molecule of one embodiment of the present invention has a buffer solution chamber receiving a buffer solution, a separation electrode arranged in the buffer solution chamber, a biological molecule separation medium for separating a biological molecule, a biological molecule adsorption film for adsorbing a biological molecule, a pair of transfer electrodes having a plurality of line shaped conductors extending in a direction perpendicular to a first direction, the conductors being arranged in the direction perpendicular to the first direction, and a separation part which holds the transfer electrode, the biological molecule adsorption film, the medium and the transfer electrode, wherein the transfer electrode, the biological molecule adsorption film, the medium and the transfer electrode are stacked in this order.

摘要翻译： 本发明的一个实施方案的分离生物分子的生物分子分离装置具有接收缓冲溶液的缓冲溶液室，配置在缓冲溶液室中的分离电极，分离生物分子的生物分子分离介质，生物分子分离装置 用于吸附生物分子的分子吸附膜，具有沿与第一方向垂直的方向延伸的多个线状导体的一对转移电极，导体沿垂直于第一方向的方向排列，分离部保持 转移电极，生物分子吸附膜，介质和转移电极，其中转移电极，生物分子吸附膜，介质和转移电极按此顺序堆叠。

公开(公告)号：US20110233062A1

公开(公告)日：2011-09-29

申请号：US13073835

申请日：2011-03-28

申请人： Koji Sakairi ,  Satonari Akutsu ,  Atsunori Hiratsuka ,  Kenji Yokoyama ,  Yuji Maruo ,  Hiroshi Ohki

发明人： Koji Sakairi ,  Satonari Akutsu ,  Atsunori Hiratsuka ,  Kenji Yokoyama ,  Yuji Maruo ,  Hiroshi Ohki

IPC分类号： C25B7/00

CPC分类号： G01N27/44769

摘要： A biological molecule separation apparatus for separating a biological molecule of one embodiment of the present invention has a buffer solution chamber receiving a buffer solution, a separation electrode arranged in the buffer solution chamber, a biological molecule separation medium for separating a biological molecule, a biological molecule adsorption film for adsorbing a biological molecule, a pair of transfer electrodes having a plurality of line shaped conductors extending in a direction perpendicular to a first direction, the conductors being arranged in the direction perpendicular to the first direction, and a separation part which holds the transfer electrode, the biological molecule adsorption film, the medium and the transfer electrode, wherein the transfer electrode, the biological molecule adsorption film, the medium and the transfer electrode are stacked in this order.

摘要翻译： 本发明的一个实施方案的分离生物分子的生物分子分离装置具有接收缓冲溶液的缓冲溶液室，配置在缓冲溶液室中的分离电极，分离生物分子的生物分子分离介质，生物分子分离装置 用于吸附生物分子的分子吸附膜，具有沿与第一方向垂直的方向延伸的多个线状导体的一对转移电极，导体沿垂直于第一方向的方向排列，分离部保持 转移电极，生物分子吸附膜，介质和转移电极，其中转移电极，生物分子吸附膜，介质和转移电极按此顺序堆叠。