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公开(公告)号：US09982314B2
公开(公告)日：2018-05-29
申请号：US15028577
申请日：2014-10-14
发明人： Hao Xu , Fei Tang , Peijun Zhan , Jianhong Hu , Huijun Ren , Hongmei Fan , Linghui Zheng , Hua Bai
摘要： A bacterial strain of Actinoplanes sp. and and the use thereof. The bacterial strain is named Actinoplanes sp. HS-16-20, whose preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 7294. The bacterial strain can be used to generate Fidaxomicin or analogs thereof or compositions containing Fidaxomicin, such as by aerobically fermenting the strain in nutrient medium containing assimilable carbon and/or nitrogen sources. Fidaxomicin has an inhibitory effect on various gram positive bacteria pathogens, and in particular, on Clostridium difficile.
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2.发明申请公开(公告)号：US20170013837A1
公开(公告)日：2017-01-19
申请号：US15123827
申请日：2014-04-28
发明人： Jun Huang , Jidong Wang , Anliang Chen , Aiwen Deng , Jiatan Lin , Zhen YU , Meihong Li , Na Li , Haibin Wang , Linghui Zheng , Hua Bai
CPC分类号： A01N43/90 , C12N15/76 , C12P19/623 , C12R1/465
摘要： The present invention relates to a recombinant microorganism expressing avermectin or analogues thereof and construction method thereof, and also relates to a method of producing avermectin or analogues thereof using the recombinant microorganism, and avermectin or analogues thereof obtained using the method. In addition, the present invention further relates to uses of the avermectin or analogues thereof as insecticides. Using the recombinant microorganism of the present invention to produce avermectin or analogues thereof has numerous advantages, for example comprising at least one of the following: good stability, high yield, simple process, environmentally friendly, and greatly saving of production costs.
摘要翻译： 本发明涉及表达除虫菌素或其类似物的重组微生物及其构建方法，还涉及使用该重组微生物生产除虫菌素或其类似物的方法，以及使用该方法得到的除虫菌素或其类似物。 此外，本发明还涉及除虫菌素或其类似物作为杀虫剂的用途。 使用本发明的重组微生物来生产除虫菌素或其类似物具有许多优点，例如包括以下至少一种：良好的稳定性，高产率，简单的工艺，环保且大大节省生产成本。
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公开(公告)号：US20210032606A1
公开(公告)日：2021-02-04
申请号：US16301377
申请日：2017-05-08
发明人： Min Zhou , Xing Jiang , Shuang Han , Yingying He , Linghui Zheng
摘要： The present invention relates to a DoxA protein mutant having an amino acid sequence set forth in SEQ ID No. 16, and coding gene thereof. The protein mutant or the coding gene thereof can be used for producing epirubicin. The present invention further relates to a Streptomyces capable of efficiently expressing epirubicin, which is constructed by replacing the dnmV gene of a starting Streptomyces in situ with the avrE gene and mutating the doxA gene of the starting Streptomyces into a gene encoding the protein set forth in SEQ ID No. 16. The fermentation broth of this Streptomyces has an epirubicin potency of up to 102.0 μg/ml.
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公开(公告)号：US10316052B2
公开(公告)日：2019-06-11
申请号：US15324555
申请日：2015-07-07
发明人： Huijun Ren , Daochao Li , Xuexiao Ying , Feng Chen , Linghui Zheng , Lingping Wang , Hua Bai
摘要： A fidaxomicin purification method, comprising: fermenting Actinoplanes sp. HS-16-20 to generate fermented liquid; conducting solid/liquid separation on the fermented liquid, soaking mycelium in an organic solvent, and filtering to obtain a solution containing fidaxomicin; treating the solution with nanofiltration concentrate, and separating to obtain fidaxomicin crude product; conducting preparative column chromatography on the fidaxomicin crude product, eluting with an acid aqueous solution containing an organic solvent, and separating to obtain the refined fidaxomicin product.
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公开(公告)号：US11371026B2
公开(公告)日：2022-06-28
申请号：US16301377
申请日：2017-05-08
发明人： Min Zhou , Xing Jiang , Shuang Han , Yingying He , Linghui Zheng
摘要： The present invention relates to a DoxA protein mutant having an amino acid sequence set forth in SEQ ID NO: 16, and coding gene thereof. The protein mutant or the coding gene thereof can be used for producing epirubicin. The present invention further relates to a Streptomyces capable of efficiently expressing epirubicin, which is constructed by replacing the dnmV gene of a starting Streptomyces in situ with the avrE gene and mutating the doxA gene of the starting Streptomyces into a gene encoding the protein set forth in SEQ ID NO: 16. The fermentation broth of this Streptomyces has an epirubicin potency of up to 102.0 μg/ml.
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公开(公告)号：US10334850B2
公开(公告)日：2019-07-02
申请号：US15123827
申请日：2014-04-28
发明人： Jun Huang , Jidong Wang , Anliang Chen , Aiwen Deng , Jiatan Lin , Zhen Yu , Meihong Li , Na Li , Haibin Wang , Linghui Zheng , Hua Bai
摘要： The present invention relates to a recombinant microorganism expressing avermectin or analogs thereof and construction method thereof, and also relates to a method of producing avermectin or analogs thereof using the recombinant microorganism, and avermectin or analogs thereof obtained using the method. In addition, the present invention further relates to uses of the avermectin or analogs thereof as insecticides. Using the recombinant microorganism of the present invention to produce avermectin or analogs thereof has numerous advantages, for example comprising at least one of the following: good stability, high yield, simple process, environmentally friendly, and greatly saving of production costs.
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公开(公告)号：US20150299241A1
公开(公告)日：2015-10-22
申请号：US14646202
申请日：2013-10-25
发明人： Hua Chen , Zhiqiang Li , Linghui Zheng , Shuyi Lu , Wei Yan , Feng Chen , Lingping Wang , Hua Bai
IPC分类号： C07H1/06 , C07H15/252
CPC分类号： C07H1/06 , C07H15/252
摘要： The present invention relates to a method for preparing highly pure doxorubicin. The method comprises the following steps: (1) chromatographing a prepurified doxorubicin solution by using macroporous resin, pre-washing the chromatographed system by using an acidic low concentration aqueous organic solvent, and performing elution by using an acidic high concentration aqueous organic solvent; (2) performing chromatographic separation on eluted components by using a preparative column, so that a highly pure doxorubicin component can be obtained, and the doxorubicin can, if needed, be separated from the aqueous solution by using conventional concentration and crystallization methods in the prior art. The present invention has the advantages such as simplicity, high yield, low cost, and less environmental pollution. The content of the prepared doxorubicin is greater than 99.5%, the content of each impurity is controlled to be lower than 0.10%, and the USP and EP standards are met.
摘要翻译： 本发明涉及制备高纯度多柔比星的方法。 该方法包括以下步骤：（1）通过使用大孔树脂对预纯化的多柔比星溶液进行色谱分离，使用酸性低浓度有机有机溶剂对色谱系统进行预洗涤，并使用酸性高浓度有机有机溶剂进行洗脱; （2）使用制备柱对洗脱成分进行色谱分离，从而可以获得高纯度的多柔比星组分，如果需要，可以通过使用常规的浓缩和结晶方法将多柔比星与水溶液分离 艺术。 本发明具有简单，成品率高，成本低，环境污染少等优点。 制备的多柔比星的含量大于99.5％，每种杂质的含量控制在0.10％以下，符合USP和EP标准。
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