公开(公告)号：US10441957B2

公开(公告)日：2019-10-15

申请号：US15103102

申请日：2014-12-05

Applicant: BIONEER CORPORATION

Inventor： Han Oh Park ,  Jong Kab Kim

IPC: B03C1/01 ,  C12N15/10 ,  G01N1/40 ,  B03C1/28 ,  B03C1/033

Abstract: The present invention relates to a magnetic particle separating device, and a method of separating and purifying nucleic acid or protein using the same. The device comprises: induction magnets (100); an induction magnet fixing part (200) having induction magnet fixing holes (210) for fixing the induction magnets (100); and a body (300) in which entry holes (310) are formed into which tubes (T) are inserted. Thus, the application and removal of a magnetic field to and from the body is made very convenient, so that the device can be very advantageously used in the separation and purification of nucleic acid or protein.

公开(公告)号：US10287542B2

公开(公告)日：2019-05-14

申请号：US14648891

申请日：2013-11-29

Applicant: BIONEER CORPORATION

Inventor： Han Oh Park ,  Jong Kab Kim ,  Ji Won Han ,  You Sang Cho ,  Min Jung Kim ,  Ha Neul Kim ,  Yang Won Lee ,  Nam Il Kim

IPC: C07K1/14 ,  C12M1/00 ,  C12M1/24 ,  C12M1/32 ,  C12M1/26 ,  C12M1/34 ,  C12P21/00 ,  C07K1/02 ,  C07K1/04 ,  C07K1/22 ,  C07K1/36

Abstract: An automated cell-free protein production system comprises: a protein expression reaction unit comprising a reaction vessel that includes a plurality of dialysis tubes, each including a dialysis membrane and being open at its top; a reaction temperature control unit configured to heat or cool the reaction vessel; a pipette array comprising a plurality of pipettes and configured to suck or discharge solutions using the pipettes; a pipette array moving unit configured to move the pipette array in an upward and downward direction, a forward and backward direction or a left and right direction so as to move solutions; a protein purification unit including a magnetic field application device; and a multi-well plate mounting unit having mounted therein a multi-well plate kit configured to supply solutions that are used for protein production.

公开(公告)号：US10022717B2

公开(公告)日：2018-07-17

申请号：US14875246

申请日：2015-10-05

Applicant: BIONEER CORPORATION

Inventor： Han Oh Park ,  Gu Young Song ,  Jung A Bae

IPC: B01L1/00 ,  B01L3/00 ,  G01N21/64 ,  C12M1/32

Abstract: A method of manufacturing a micro-chamber plate with a built-in sample, including: settling a micro-chamber plate for sample injection at a micro-chamber plate receiving part formed with an upper opening; disposing a cover for micro-chamber plate receiving part to cover the upper opening, the cover for micro-chamber plate receiving part having a provisional storing part and an auxiliary covering part connected with the provisional storing part and formed with a through-hole for auxiliary covering part; and manufacturing a micro-chamber plate with a built-in sample by putting the micro-chamber plate receiving part, on which the cover is disposed, into a centrifugal separator which can apply vacuum, applying centrifugal force and injecting a sample solution provisionally stored in the provisional storing part into the micro-chamber plate through a vessel communication part which is formed at the provisional storing part to be communicated with the micro-chamber plate receiving part.

公开(公告)号：US20170152512A1

公开(公告)日：2017-06-01

申请号：US14902563

申请日：2014-07-04

Applicant: BIONEER CORPORATION

Inventor： Han Oh Park ,  Jeiwook Chae ,  Pyoung Oh Yoon ,  Boram Han ,  Gi-Eun Choi ,  Youngho Ko ,  Taewoo Kwon ,  Jae Don Lee ,  Sun Gi Kim

IPC: C12N15/113 ,  A61K31/713

CPC classification number: C12N15/113 ,  A61K9/127 ,  A61K9/5123 ,  A61K31/713 ,  A61K47/54 ,  A61K47/6935 ,  C07H21/00 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/351 ,  C12N2310/3515 ,  C12N2320/32 ,  C12N2330/30 ,  Y02A50/473

Abstract: The present invention relates to an oligonucleotide structure and a method for preparing the same and, more particularly, to an oligonucleotide structure in which a polymer compound is linked to an oligonucleotide via a covalent bond to improve in vivo stability of the oligonucleotide and cellular delivery efficiency of the oligonucleotide; and to a method for preparing the same. The oligonucleotide structure is improved into a homogenous material, thereby solving the problem in material verification due to polydispersion characteristics occurring when a hydrophilic material linked to the oligonucleotide is a synthetic polymer; the oligonucleotide structure is easy to synthesize compared with the existing process; and the size of a double-stranded oligo RNA structure can be accurately adjusted through the control of the repetition number of a hydrophilic material block, and thus, the gene expression regulation function of the oligonucleotide does not deteriorate through the synthesis of the optimized oligonucleotide structure, and the oligonucleotide can be delivered into cells at even a relatively low-concentration dosage. Therefore, the oligonucleotide structure of the present invention can be useful as a novel type oligonucleotide delivery system as well as a tool for treating cancers, infectious diseases, and the like.

公开(公告)号：US09649388B2

公开(公告)日：2017-05-16

申请号：US14372211

申请日：2013-01-17

Applicant: BIONEER CORPORATION

Inventor： Jeiwook Chae ,  Hyunjin Chung ,  Boreum Lee ,  Han Oh Park

IPC: A61K47/48 ,  C12N15/11 ,  A61K49/18 ,  A61K9/14 ,  A61K31/7105 ,  A61K9/51 ,  A61K31/713 ,  A61K33/24 ,  A61K9/50

CPC classification number: A61K47/48015 ,  A61K9/14 ,  A61K9/5094 ,  A61K9/5115 ,  A61K31/7105 ,  A61K31/713 ,  A61K33/24 ,  A61K47/6923 ,  A61K49/1857 ,  C12N15/11 ,  A61K2300/00

Abstract: Provided are a SAMiRNA-magnetic nanoparticle complex capable of effectively delivering a double-stranded oligo RNA and magnetic nanoparticles into a cell and a composition capable of simultaneously performing diagnosis and therapy of diseases such as cancer, and the like, containing the same. More specifically, provided is the SAMiRNA-magnetic nanoparticle complex consisting of double-stranded oligo RNA-polymer structures in which a hydrophilic material and a second hydrophobic material are bound to the double-stranded oligo RNA by a simple covalent bond or a linker-mediated covalent bond, and the magnetic nanoparticles in which a first hydrophobic material is bound onto a surface of the magnetic material, as a core.The SAMiRNA-magnetic nanoparticle complex may have a homogeneous size by a hydrophobic interaction between the first hydrophobic material of the present invention and the second hydrophobic material of the double-stranded oligo RNA structure.In addition, the hydrophilic material and the second hydrophobic material bound to the double-stranded oligo RNA structure may improve in vivo stability of the double-stranded oligo RNA, an additionally bound ligand may deliver the SAMiRNA-magnetic nanoparticle complex into a target cell even at a relative low concentration of dosage, and the magnetic materials of the magnetic nanoparticles may be used as an imaging agent for diagnosis.

公开(公告)号：US20160145622A1

公开(公告)日：2016-05-26

申请号：US14902564

申请日：2014-07-04

Applicant: BIONEER CORPORATION

Inventor： Joo Sung Yang ,  Woo Seok Kim ,  Soon Ja Choi ,  Han Oh Park

IPC: C12N15/113

CPC classification number: C12N15/1131 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/351 ,  C12N2310/3515 ,  C12N2310/3521 ,  C12N2310/3525 ,  C12N2310/3533

Abstract: The present invention relates to a dengue virus-specific siRNA, a double-stranded oligo RNA structure comprising the siRNA, and a composition for inhibiting dengue virus replication, which comprises the same, in which the double-stranded oligo RNA structure comprises a hydrophilic compound and hydrophobic compound conjugated to both ends of the double-stranded RNA (siRNA) by a single covalent bond or a linker-mediated covalent bond so that they will be efficiently delivered into cells, and can be converted into nanoparticles by hydrophobic interactions between the double-stranded oligo RNA structures in an aqueous solution. The siRNA included in the double-stranded oligo RNA structure acts specifically on all dengue virus serotypes. The present invention also relates to a method for preparing the double-stranded oligo RNA structure, and a pharmaceutical composition for preventing or treating dengue virus infection, which comprises the double-stranded oligo RNA structure.

Abstract translation: 本发明涉及登革病毒特异性siRNA，包含siRNA的双链寡聚RNA结构和用于抑制登革病毒复制的组合物，其包含其中双链寡RNA结构包含亲水化合物 和通过单个共价键或连接体介导的共价键与双链RNA（siRNA）的两端缀合的疏水性化合物，使得它们将被有效地递送到细胞中，并且可以通过双重的疏水相互作用转化成纳米颗粒 在水溶液中的品系寡聚RNA结构。 包含在双链寡聚RNA结构中的siRNA特异性地对所有登革热病毒血清型起作用。 本发明还涉及制备双链寡聚RNA结构的方法，以及包含双链寡聚RNA结构的用于预防或治疗登革病毒感染的药物组合物。

公开(公告)号：US09211343B2

公开(公告)日：2015-12-15

申请号：US14291540

申请日：2014-05-30

Applicant: Bioneer Corporation

Inventor： Bo Ram Han ,  Han Oh Park ,  Mi Sik Shin ,  Sam Young Lee

IPC: A61K47/00 ,  A61K9/107 ,  A61K47/48 ,  C07H21/02 ,  C12N15/11 ,  C08G65/335 ,  C07F7/08 ,  C12N15/113 ,  B82Y5/00 ,  A61K47/34

CPC classification number: A61K47/48215 ,  A61K9/1075 ,  A61K47/34 ,  A61K47/60 ,  B82Y5/00 ,  C07F7/0836 ,  C07H21/02 ,  C08G65/3356 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/351 ,  C12N2310/3515 ,  C12N2320/30 ,  C12N2320/51 ,  Y10S977/773 ,  Y10S977/906

Abstract: Provided are an siRNA-polymer conjugate, and a method for preparing the same, and more specifically, to a hybrid conjugate formed by covalently bonding siRNA and a polymeric compound for improving the in vivo stability of siRNA, and to a preparation method of the hybrid conjugate. The conjugate of the present invention can improve the in vivo stability of siRNA, thereby achieving an efficient delivery of therapeutic siRNA into cells and exhibiting the activity of siRNA even with a small dose of a relative low concentration. Therefore, the conjugate can advantageously be used as not only an siRNA treatment tool for cancers and other infectious disease, but also a novel type siRNA delivery system.

Abstract translation: 提供了siRNA-聚合物缀合物及其制备方法，更具体地，涉及通过共价键合siRNA和高分子化合物形成的用于提高siRNA体内稳定性的杂合偶联物，以及该混合物的制备方法 缀合物。 本发明的缀合物可以提高siRNA的体内稳定性，从而实现治疗性siRNA有效递送到细胞中，并且即使用相对低浓度的小剂量也显示出siRNA的活性。 因此，缀合物不仅可以有效地用作癌症和其它感染性疾病的siRNA治疗工具，还可以用作新型siRNA递送系统。

公开(公告)号：US20140371432A1

公开(公告)日：2014-12-18

申请号：US14365621

申请日：2012-12-14

Applicant: Bioneer Corporation

Inventor： Jeiwook Chae ,  Boram Han ,  Han-na Kim ,  Han Oh Park ,  Pyoung Oh Yoon ,  Sun Gi Kim ,  Kwang-Ju Jung ,  Taewoo Kwon ,  Jong Deok Choi ,  Sam Young Lee ,  Eun-Jung Jung

IPC: A61K47/42 ,  C12N15/113 ,  A61K47/22 ,  A61K31/713

CPC classification number: A61K47/42 ,  A61K31/713 ,  A61K47/549 ,  A61K47/551 ,  A61K47/60 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/3513 ,  C12N2310/3515 ,  C12N2320/32

Abstract: The present invention provides a double-stranded RNA structure, which comprises a polymer compound covalently bonded to a double-helix oligo RNA useful for the treatment of diseases, particularly cancer, in order to enhance the delivery of the double-helix oligo RNA, and further comprises a target-specific ligand bonded thereto, a preparation method thereof, and a technique of delivering the double-helix oligo RNA in a target-specific manner using the RNA structure. A nanoparticle composed of the ligand-bonded double-helix oligo RNA structures can efficiently deliver the double-helix oligo RNA to a target, and thus can exhibit the activity of the double-helix oligo RNA even when the double-helix oligo RNA is administered at a relatively low concentration. Also, it can prevent the non-specific delivery of the double-helix oligo RNA into other organs and cells. Accordingly, the ligand-bonded double-stranded RNA structure can be used for the treatment for various diseases, particularly cancer, and can also be effectively used as a new type of double-helix oligo RNA delivery system. Particularly, the ligand-bonded double-stranded RNA structure can be effectively used for the treatment of diseases, including cancer and infectious diseases. Moreover, the present invention relates to a hybrid conjugate, which comprises a hydrophilic material and hydrophobic material bonded to both ends of an antisense oligonucleotide (ASO) by a covalent bond in order to enhance the in vivo stability of the ASO, a method for preparing the hybrid conjugate, and a nanoparticle composed of the conjugates. The ASO-polymer conjugate according to the invention can increase the in vivo stability of the ASO, making it possible to efficiently deliver the therapeutic ASO into cells. Also, the ASO-polymer conjugate can exhibit the activity of the ASO even when it is administered at a relatively low concentration.

Abstract translation: 本发明提供双链RNA结构，其包含与用于治疗疾病，特别是癌症的双螺旋寡聚RNA共价键合的高分子化合物，以增强双螺旋寡聚RNA的递送，以及 还包括与其键合的靶特异性配体，其制备方法，以及使用RNA结构以目标特异性方式递送双螺旋寡聚RNA的技术。 由配体结合的双螺旋寡聚RNA结构组成的纳米粒子可以有效地将双螺旋寡聚RNA递送至靶，因此即使施用双螺旋寡核苷酸RNA也能显示双螺旋寡聚RNA的活性 浓度相对较低。 此外，它可以防止双螺旋寡聚RNA非特异性递送到其他器官和细胞中。 因此，配体键合的双链RNA结构可以用于各种疾病，特别是癌症的治疗，也可以有效地用作新型双螺旋寡聚RNA递送系统。 特别地，配体键合的双链RNA结构可以有效地用于治疗包括癌症和感染性疾病在内的疾病。 此外，本发明涉及一种杂合缀合物，其包含通过共价键与反义寡核苷酸（ASO）的两端键合的亲水性材料和疏水性材料，以增强ASO的体内稳定性，制备方法 杂交缀合物和由缀合物组成的纳米颗粒。 根据本发明的ASO-聚合物缀合物可以增加ASO的体内稳定性，使得可以有效地将治疗性ASO递送到细胞中。 此外，即使以相对低的浓度施用，ASO-聚合物缀合物也可以表现出ASO的活性。

公开(公告)号：US20220176373A1

公开(公告)日：2022-06-09

申请号：US17602665

申请日：2020-04-09

Applicant: Bioneer Corporation

Inventor： Han Oh Park ,  Jong Kab Kim ,  Yang Won Lee ,  Sang Ryoung Park ,  Hye Jin Jang

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/6806

Abstract: The present invention relates to a system structure capable of performing real-time detection of nucleic acid extraction and amplification reactions and amplified results in a device for implementing a polymerase chain reaction (PCR). A PCR system includes a nucleic acid extraction cartridge configured to extract a nucleic acid of a biological sample via a nucleic acid extraction reagent stored therein and form a PCR preliminary mixture by being additionally mixed with a polymerase reaction dried product, a PCR plate inserted into the nucleic acid extraction cartridge, having a channel coupled to the nucleic acid extraction cartridge, and receives a nucleic acid solution or the PCR preliminary mixture extracted from the nucleic acid extraction cartridge and diversely accommodates a PCR reaction dried product containing a dried primer/probe or a primer/probe in at least one reaction well, a temperature control module disposed above the PCR plate 200 and including a pair of heating blocks 310 and 320 adjacent to the reaction well (W) to apply different temperatures, and a scanning module scanning a concentration of a reactant amplified in the reaction well (W).

公开(公告)号：US11175300B2

公开(公告)日：2021-11-16

申请号：US16338218

申请日：2017-09-28

Applicant: BIONEER CORPORATION

Inventor： Han Oh Park ,  Yang Won Lee ,  Jin Il Lee ,  Jung Do Kim ,  Dae Jin Jang ,  Jong Kab Kim

IPC: G01N35/04 ,  G01N35/10 ,  G01N35/00 ,  G01N35/02 ,  B01L3/02

Abstract: A biological sample processing apparatus, including: a pipette block with which a plurality of pipettes for sucking or discharging a biological sample in a multi-well plate in which wells are arranged in a matrix shape along row and column directions are detachably coupled; a pipette block forward and backward transfer unit configured to move the pipette block along a forward and backward direction along a process direction; a pipette block top and bottom transfer unit configured to move the pipette block along a vertical direction; a magnetic field applying unit disposed below the multi-well plate for applying a magnetic field to a well of the multi-well plate; and a heating unit disposed below the multi-well plate so as to be spaced apart from the magnetic field applying unit, for heating a well of the multi-well plate.