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公开(公告)号：US12105049B2
公开(公告)日：2024-10-01
申请号：US17739251
申请日：2022-05-09
发明人： Andreas Haase
IPC分类号： G01N27/62 , G01N27/623 , H01J49/00 , H01J49/16 , H01J49/40
CPC分类号： G01N27/623 , H01J49/0031 , H01J49/164 , H01J49/40
摘要： Disclosed is a method for analytically measuring sample material deposited on a sample support surface, comprising: (a) defining a plurality of regions on the surface, several of which are in contact with sample material, (b1) sampling sections of sample on a region using a desorbing beam to generate desorbed molecules, which are ionized and transferred to an analyzer, (b2) in so doing, sweeping the region by changing an orientation setting of the beam relative to the surface along a non-rectilinear trajectory on the region selected from a plurality of predefined, non-rectilinear trajectories while keeping the support in one position, (c) transitioning from a swept region to a region to be swept next using spatial adjustment of the support, and (d) repeating steps (b1), (b2), and (c) until a predetermined termination condition is fulfilled. A system for analyzing ions, having an ion generation device and a control unit is also disclosed.
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公开(公告)号：US12031989B2
公开(公告)日：2024-07-09
申请号：US17823318
申请日：2022-08-30
发明人： Karsten Becker , Evgeny Idelevich
CPC分类号： G01N33/6848 , C12Q1/18 , G01N33/6851 , G01N2560/00 , G01N2800/44
摘要： The invention relates to a method for the preparation of living, microbial samples and microorganisms for subsequent mass spectrometric measurement and evaluation. Findings which can be derived from such a measurement can particularly serve the faster identification of microorganisms in the microbial sample according to species/subspecies and/or the fast determination of resistance/sensitivity of the microorganisms to antimicrobial substances and/or the further characterization of microorganisms, for example in respect of pathogenicity, virulence and metabolism. According to a preferred embodiment of the invention, the preparation particularly takes place directly on a mass spectrometric sample support.
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3.发明授权公开(公告)号：US11982603B2
公开(公告)日：2024-05-14
申请号：US17702276
申请日：2022-03-23
发明人： Katrin Sparbier , Beatrix Wegemann
CPC分类号： G01N1/4044 , C12Q1/045 , C12Q1/18 , G01N1/28 , G01N33/6851 , G01N1/4055
摘要： The invention relates to the preparation of biological cells for the mass spectrometric analysis of cellular properties such as taxonomic classification, antibiotic resistances, response to drugs or other active substances, and others. The cells can be prokaryotic or eukaryotic microorganisms which have particularly been cultivated directly on a mass spectrometric sample support, or eukaryotic cells from tissues or cell cultures. The invention proposes that the cells are not disrupted by adding matrix solution for a subsequent ionization by matrix-assisted laser desorption (MALDI), but that they are disrupted in a separate treatment step using acids and/or solvents on the sample support itself. Surprisingly, the cell proteins released then adhere to the sample support so that they can be carefully washed with buffer solution to remove salts and other soluble impurities which can stem from earlier treatment steps, for example from nutrient solution.
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4.发明公开公开(公告)号：US20230159977A1
公开(公告)日：2023-05-25
申请号：US18056107
申请日：2022-11-16
发明人： Katrin SPARBIER
CPC分类号： C12Q1/18 , G01N33/6851 , G16B40/10 , G16B20/00
摘要： The invention relates to methods for spectrometric characterization of a test cell substrate. The characterization comprises taxonomic classification and determination of a property of interest of the test cell substrate. The characterization may be based on mass-spectrometric measurement data. The property of interest may be a resistance or susceptibility to a growth-influencing factor. After comparing first spectrometric measurement data of the test cell substrate with a provided reference library, a sub-library is created comprising those reference datasets from the reference library that are classified as allowing a taxonomic classification of the test cell substrate. Second spectrometric measurement data after a second preparation of the test cell substrate under conditions that serve to determine a property of interest of the test cell substrate is compared with the sub-library and allow a reliable determination of the property of interest.
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公开(公告)号：US20230145540A1
公开(公告)日：2023-05-11
申请号：US18051772
申请日：2022-11-01
发明人： Andreas HAASE , Marcel NIEHAUS , Jens HÖHNDORF
CPC分类号： H01J49/0418 , G06T7/70
摘要： The invention relates to devices and methods for desorption scanning of analyte material deposited on a sample support, which can comprise the following mode of operation: (a) setting a position of the support to approach an impingement region onto which a beam is directed for local desorption of analyte material; (b) determining an actual position of the support after setting the position; (c) comparing the determined actual position with a target position of the support to determine any deviation; (d) adjusting a beam orientation, if a deviation is detected, so that the beam is directed onto the impingement region on the support that results when there is no deviation; (e) applying the beam to the impingement region to locally desorb analyte material and deliver it to an analyzer; and (f) checking whether a predetermined end condition is satisfied and, if not, repeating steps (a)-(e) for a subsequent non-congruent impingement region.
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公开(公告)号：US20220397551A1
公开(公告)日：2022-12-15
申请号：US17739251
申请日：2022-05-09
发明人： Andreas HAASE
IPC分类号： G01N27/623 , H01J49/16 , H01J49/40 , H01J49/00
摘要： Disclosed is a method for analytically measuring sample material deposited on a sample support surface, comprising: (a) defining a plurality of regions on the surface, several of which are in contact with sample material, (b1) sampling sections of sample on a region using a desorbing beam to generate desorbed molecules, which are ionized and transferred to an analyzer, (b2) in so doing, sweeping the region by changing an orientation setting of the beam relative to the surface along a non-rectilinear trajectory on the region selected from a plurality of predefined, non-rectilinear trajectories while keeping the support in one position, (c) transitioning from a swept region to a region to be swept next using spatial adjustment of the support, and (d) repeating steps (b1), (b2), and (c) until a predetermined termination condition is fulfilled. A system for analyzing ions, having an ion generation device and a control unit is also disclosed.
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公开(公告)号：US20240355610A1
公开(公告)日：2024-10-24
申请号：US18634324
申请日：2024-04-12
发明人： Sebastian BÖHM , Andreas HAASE
CPC分类号： H01J49/403 , H01J49/0004 , H01J49/0418 , H01J49/164 , H01J49/405
摘要： The disclosure relates to devices and methods for the spectrometric analysis of sample material located on a sample support, and in particular on a flat sample support plate, using axial time-of-flight analysis. One operating mode of the devices and methods comprises an adjustment of the pulse focal position for the abrupt ablation and/or abrupt desorption of sample material in a z-direction that is perpendicular to a tangential plane at the location of ablation and/or desorption at the sample support, and the selection of a suitable setting for an acceleration with time lag of the ablated and/or desorbed and ionized sample material onto a flight path. It can be particularly advantageous to use these devices and methods in mass spectrometry imaging (MSI). The devices and methods can, in particular, be used with laser desorption/ionization (LDI) and specifically matrix-assisted laser desorption/ionization (MALDI).
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公开(公告)号：US12077806B2
公开(公告)日：2024-09-03
申请号：US18303204
申请日：2023-04-19
发明人： Norman Mauder
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , G01N2560/00
摘要： The invention relates to a method for the spectrometric characterization of microorganisms, comprising: providing a test microorganism; acquiring spectrometric measurement data from the test microorganism under potential exposure to variance that is not based on taxonomic classification; selecting a classifier which is trained to determine the identity of a microorganism on a second taxonomic level; and applying the classifier to the measurement data in order to determine the identity of the test microorganism on the second taxonomic level, wherein the classifier is variance-conditioned in such a way that it largely or completely masks out the effect of variance in the characterization of the test microorganism on the second taxonomic level.
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公开(公告)号：US20240177986A1
公开(公告)日：2024-05-30
申请号：US18523158
申请日：2023-11-29
发明人： Marcel NIEHAUS , Andreas HAASE
CPC分类号： H01J49/164 , H01J49/0031 , H01J49/0418
摘要： Disclosed are methods and devices for desorbing and ionizing of sample material which is deposited on a sample support, which methods and devices use a post-desorption ionization modality with dynamic spatial alignment. Principles of the disclosure may be used in imaging ion spectrometry, for example, particularly in imaging ion spectrometry with ion generation using matrix-assisted laser desorption and ionization (MALDI).
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公开(公告)号：US11705316B2
公开(公告)日：2023-07-18
申请号：US17747594
申请日：2022-05-18
IPC分类号： H01J49/00 , G01N33/483 , B01D59/44 , G01N27/64
CPC分类号： H01J49/0004 , B01D59/44 , G01N27/64 , G01N33/483
摘要： The invention relates to a method for the determination and visualization of the spatial distribution of tissue states of a tissue sample, wherein a mass/mobility map is acquired at each of a plurality of sample sites of the tissue sample, the signal heights at each sample site are determined at characteristic signal positions in the corresponding mass/mobility map, from which a tissue state for each sample site is calculated with the aid of a mathematical/statistical classification algorithm, and the spatial distribution of the tissue states calculated for the sample sites is represented graphically.
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