公开(公告)号：US20100309548A1

公开(公告)日：2010-12-09

申请号：US12680265

申请日：2008-09-18

申请人： Christopher Power ,  Helmut Lippert ,  Dietrich Christian ,  Benno Radt

发明人： Christopher Power ,  Helmut Lippert ,  Dietrich Christian ,  Benno Radt

IPC分类号： G02B21/06

CPC分类号： G02B21/361 ,  G02B21/008 ,  G02B21/06

摘要： A method and an arrangement for analyzing a specimen, wherein the specimen is supported so as to be rotatable around an axis of rotation and displaceable in all three spatial directions and is illuminated by a first illumination device. Light radiated from the specimen is imaged on a detection device. A plurality of sectional images of the specimen are recorded at different settings of the rotational angle, and the specimen is rotated. The recorded sectional images are fused to form a data set of spatial image data of the specimen. The specimen is then illuminated by a second illumination device perpendicular to the axis of rotation, wherein a plurality of shadow images of the specimen are recorded and the specimen is rotated. A second data set of spatial image data of the specimen is constructed from the recorded shadow images by means of a back projection algorithm.

摘要翻译： 一种用于分析样本的方法和装置，其中所述样本被支撑成可围绕旋转轴线旋转并且可在所有三个空间方向上移位并被第一照明装置照射。 从样品辐射的光在检测装置上成像。 在旋转角度的不同设定下记录试样的多个截面图像，并且使样本旋转。 记录的截面图像被融合以形成样本的空间图像数据的数据集。 然后通过垂直于旋转轴线的第二照明装置照射样本，其中记录样本的多个阴影图像并且样本旋转。 通过背投影算法从记录的阴影图像中构建样本的空间图像数据的第二数据集。

公开(公告)号：US20080049313A1

公开(公告)日：2008-02-28

申请号：US11844441

申请日：2007-08-24

申请人： Michael Brehm ,  Werner Kleinschmidt ,  Jan Thirase

发明人： Michael Brehm ,  Werner Kleinschmidt ,  Jan Thirase

IPC分类号： G02B21/06

CPC分类号： G02B21/16 ,  G01N21/648 ,  G02B2207/113

摘要： The invention is directed to illumination optics for an optical device for observing a sample, particularly for TIRF microscopy (Total Internal Reflection Fluorescence Microscopy), wherein the sample is positioned on the side of a carrier glass remote of the illumination optics and the illumination light exiting from the illumination optics is shaped into an illumination beam bundle which encloses an angle not equal to 90° with the normal to the surface of the carrier glass. According to the invention, the illumination optics of the type mentioned above comprise at least two optically active elements which influence the shape and direction of the illumination beam bundle and which are arranged outside of the detection beam path that guides light coming from the sample to a detector. The optically active elements are preferably constructed as annular lenses and are arranged concentrically around the detection beam path.

摘要翻译： 本发明涉及用于观察样品的光学装置的照明光学器件，特别是用于TIRF显微镜（全内反射荧光显微镜）的样品，其中样品位于远离照明光学器件的载体玻璃侧，并且照射光离开 从照明光学器件被成形为照射束束，其包围与载体玻璃的表面垂直的不等于90°的角度。 根据本发明，上述类型的照明光学器件包括影响照明束束的形状和方向的至少两个光学有源元件，它们被布置在检测光束路径的外部，该光束路径将来自样品的光引导到 探测器。 光学活性元件优选构造为环形透镜并且围绕检测光束路径同心地布置。

公开(公告)号：US20070127116A1

公开(公告)日：2007-06-07

申请号：US11566078

申请日：2006-12-01

申请人： PETER DIETRICH ,  Cornelia Bendlin ,  Andreas Hermann

发明人： PETER DIETRICH ,  Cornelia Bendlin ,  Andreas Hermann

IPC分类号： G02B21/00

CPC分类号： G02B21/08 ,  G02B21/24

摘要： The subject matter of the invention is an arrangement for focusing and adjusting illumination devices at microscopes which comprises a stationary stage and a housing or a stand with a stand base. A condenser carrier is arranged at the stand base so as to be removable and carries a condenser holder which is mounted so as to be displaceable in direction of the optical axis of the microscope beam path and at which a condenser is arranged. Further, a device is provided at the condenser holder for exchanging and adjusting the condenser. According to the invention, the condenser carrier is arranged between the observer and the optical axis of the microscope beam path in the area of the stand base adjacent to (facing) the observer.

摘要翻译： 本发明的主题是用于在显微镜下聚焦和调节照明装置的装置，其包括固定台和具有支架底座的壳体或支架。 在支架底座上布置有一个电容器托架，以便可移动，并带有一个冷凝器支架，该冷凝器支架安装成可在显微镜光束路径的光轴方向上移位，并在其上布置冷凝器。 此外，在冷凝器保持器处设置有用于更换和调节冷凝器的装置。 根据本发明，在与观察者相邻（面向）的支架底座的区域中，在观察者和显微镜光束路径的光轴之间布置了聚光镜载体。

公开(公告)号：US20060268270A1

公开(公告)日：2006-11-30

申请号：US11382584

申请日：2006-05-10

申请人： Felix Kerstan ,  Nico Correns

发明人： Felix Kerstan ,  Nico Correns

IPC分类号： G01J3/28

CPC分类号： G01J3/10 ,  G01J3/02 ,  G01J3/027 ,  G01J3/24 ,  G01J3/36 ,  G01J2003/1286

摘要： The present invention is directed to a grating spectrometer system for polychromator spectrometer arrangements and monochromator spectrometer arrangements. The grating spectrometer system, according to the invention, comprises a light source for illuminating the sample to be analyzed, a diffraction grating, imaging optical elements, a detector arranged in the image plane, and a controlling and regulating unit. Individual light sources, preferably LEDs having different spectral characteristics, whose spectral range covers a plurality of diffraction orders in the image plane are used as light source. Only those LEDs which do not illuminate the same location of the individual detectors arranged in the image plane in any diffraction order are switched on individually or in groups by the controlling and regulating unit. The proposed solution is suitable for polychromator spectrometer arrangements and for monocluomator spectrometer arrangements. The field of application is determined by the spectral sensitivity of the detector that is employed. By using a plurality of diffraction orders, the resolution can be increased with the detector size remaining the same, or the detector surface can be reduced while retaining the same imaging quality.

摘要翻译： 本发明涉及一种用于多色光谱仪布置和单色仪光谱仪布置的光栅光谱仪系统。 根据本发明的光栅光谱仪系统包括用于照射待分析样品的光源，衍射光栅，成像光学元件，布置在图像平面中的检测器以及控制和调节单元。 各个光源，优选具有不同光谱特性的LED，其光谱范围覆盖图像平面中的多个衍射级，被用作光源。 只有那些不以任何衍射级布置在图像平面中的各个检测器的相同位置照明的那些LED由控制和调节单元单独或分组地接通。 所提出的解决方案适用于多色谱仪布置和用于单色器光谱仪布置。 应用领域由采用的检测器的光谱灵敏度决定。 通过使用多个衍射级，可以在检测器尺寸保持相同的情况下提高分辨率，或者可以减小检测器表面，同时保持相同的成像质量。

公开(公告)号：US20130094755A1

公开(公告)日：2013-04-18

申请号：US13705237

申请日：2012-12-05

申请人： Carl Zeiss Microlmaging GmbH

发明人： Helmut Lippert ,  Benno Radt ,  Michael Kempe ,  Christian Dietrich

IPC分类号： G06T7/00

CPC分类号： G06T7/97 ,  G02B21/002 ,  G02B21/244 ,  G02B21/367

摘要： A method for the three-dimensional imaging of a sample in which image information from different depth planes of the sample is stored in a spatially resolved manner, and the three-dimensional image of the sample is subsequently reconstructed from this stored image information is provided. A reference structure is applied to the illumination light, at least one fluorescing reference object is positioned next to or in the sample, images of the reference structure of the illumination light, of the reference object are recorded from at least one detection direction and evaluated. The light sheet is brought into an optimal position based on the results and image information of the reference object and of the sample from a plurality of detection directions is stored. Transformation operators are obtained on the basis of the stored image information and the reconstruction of the three-dimensional image of the is based on these transformation operators.

摘要翻译： 提供了一种用于样本的三维成像的方法，其中以空间分辨的方式存储来自样本的不同深度平面的图像信息，并且随后从该存储的图像信息重建样本的三维图像。 参考结构被应用于照明光，至少一个发荧光参考对象被放置在样本旁边或样本中，从至少一个检测方向记录参考物体的照明光的参考结构的图像并进行评估。 基于结果使光照片进入最佳位置，存储参考对象的图像信息和来自多个检测方向的样本的图像信息。 基于存储的图像信息和基于这些变换算子的三维图像的重建获得转换算子。

公开(公告)号：US20120205519A1

公开(公告)日：2012-08-16

申请号：US13435628

申请日：2012-03-30

申请人： Gunter Möhler ,  Dietmar Schmidt ,  Oliver Holub

发明人： Gunter Möhler ,  Dietmar Schmidt ,  Oliver Holub

IPC分类号： G01J1/44

CPC分类号： G02B21/008 ,  G01J1/20 ,  G01J1/42

摘要： Method and apparatus for generating an at least two-dimensional image of at least part of a sample. The method involves scanning the sample. Acquiring at least one light signal by an optoelectronic detector for different areas of the sample. Converting the light signal into an electrical signal. Distributing the electrical signal onto several parallel evaluation channels whose signal evaluations differ from each other so that their dynamic ranges are different. Generating a result signal in each evaluation channel. Selecting at least one of the result signals as a function of one of the result signals in order to generate the image for the sample range concerned. It is also possible to generate one intermediate result signal for each channel, typically from the respective actual result signal and one or more other sources. Thus the signal selection depending on both the result signals and the intermediate result signals are possible.

摘要翻译： 用于产生至少部分样品的至少二维图像的方法和装置。 该方法涉及扫描样品。 通过光电检测器获取至少一个光信号，以获得样品的不同区域。 将光信号转换为电信号。 将电信号分配到信号评估彼此不同的几个并行评估通道上，使得它们的动态范围不同。 在每个评估通道中生成结果信号。 选择结果信号中的至少一个作为结果信号之一的函数，以便生成所关注的样本范围的图像。 通常也可以从相应的实际结果信号和一个或多个其他源产生每个信道的一个中间结果信号。 因此，取决于结果信号和中间结果信号的信号选择是可能的。

公开(公告)号：US20110317258A1

公开(公告)日：2011-12-29

申请号：US13255627

申请日：2010-03-06

申请人： Ralf Wolleschensky

发明人： Ralf Wolleschensky

IPC分类号： G02B21/06

CPC分类号： G02B21/0076 ,  G01J3/02 ,  G01J3/0237 ,  G01J3/26 ,  G01J3/36 ,  G01J3/4406 ,  G01J2003/1221 ,  G01N21/645 ,  G01N2021/6471 ,  G02B21/0064 ,  G02B27/1006

摘要： Microscope, particularly laser scanning microscope, for optical detection of light radiation excited in a specimen, having a detection beam path for detecting spectral components of the light radiation in a plurality of detection channels, wherein the light radiation arrives at a variable longpass filter or shortpass filter from which reflected and/or transmitted components are reflected back with a parallel offset, and the latter arrive at a detector after at least one back-reflection of this kind.

摘要翻译： 显微镜，特别是激光扫描显微镜，用于在样本中激发的光辐射的光学检测，具有用于检测多个检测通道中的光辐射的光谱分量的检测光束路径，其中光辐射到达可变长通滤波器或短路 反射和/或透射的分量由平行偏移反射回来的滤光器，后者在这种至少一次背反射之后到达检测器。

公开(公告)号：US20080071407A1

公开(公告)日：2008-03-20

申请号：US11857866

申请日：2007-09-19

申请人： Jorg Schaffer ,  Eugen Wehner

发明人： Jorg Schaffer ,  Eugen Wehner

IPC分类号： G06F19/00

CPC分类号： G02B21/365 ,  G02B21/24

摘要： A microscope configuration according to an exemplary embodiment includes a microscope system with at least one addressable component and also a control system with a plurality of control modules for influencing a plurality of test-environment parameters in a test chamber of the microscope system. The control modules are configured to be combined in modular manner and to be coupled through an interface unit with a unified bus, through which they are controlled. A control module influencing a test-environment parameter of an incubation system has a control command interface unit configured to receive at least one control command. The control command interface unit couples with a bus. A control device is coupled with the control command interface unit and influences the test-environment parameter based upon the control command. A further interface unit is coupled to the control command interface unit and outputs, again, the received control command.

摘要翻译： 根据示例性实施例的显微镜配置包括具有至少一个可寻址部件的显微镜系统，以及具有用于影响显微镜系统的测试室中的多个测试环境参数的多个控制模块的控制系统。 控制模块被配置为以模块化方式组合并且通过与统一总线的接口单元耦合，通过它们被控制。 影响孵化系统的测试环境参数的控制模块具有被配置为接收至少一个控制命令的控制命令接口单元。 控制命令接口单元与总线耦合。 控制装置与控制命令接口单元耦合，并根据控制命令影响测试环境参数。 另一个接口单元耦合到控制命令接口单元，并再次输出接收到的控制命令。

公开(公告)号：US07855831B2

公开(公告)日：2010-12-21

申请号：US11459145

申请日：2006-07-21

申请人： Ralf Wolleschensky ,  Hans-Jürgen Dobschal

发明人： Ralf Wolleschensky ,  Hans-Jürgen Dobschal

IPC分类号： G02B21/02

CPC分类号： G02B21/02 ,  G02B21/006 ,  G02B21/0072 ,  G02B21/33

摘要： A microscope objective including an front optical element, a plurality of optical elements spaced apart from the front element and from each other, as well as an adjusting unit. At least one of the optical elements can be displaced along the optical axis by the adjusting unit to adjust the focus of the objective. The focus of the objective is displaced relative to the front element along the optical axis and/or a temperature-induced imaging error of the objective is compensated for.

摘要翻译： 包括前光学元件，与前元件间隔开并且彼此间隔开的多个光学元件的显微镜物镜以及调节单元。 至少一个光学元件可以通过调节单元沿着光轴移位，以调整物镜的焦点。 物镜的焦点相对于沿着光轴的前元件移位，和/或补偿了物镜的温度引起的成像误差。

公开(公告)号：US20080013195A1

公开(公告)日：2008-01-17

申请号：US11791913

申请日：2005-12-20

申请人： Ingo Fahlbusch ,  Frank Nolte ,  Georg Herbst

发明人： Ingo Fahlbusch ,  Frank Nolte ,  Georg Herbst

IPC分类号： G02B7/02

CPC分类号： G02B21/02 ,  G02B7/008

摘要： The invention is directed to a temperature-controllable objective, particularly for microscopes and other optical equipment, which comprises a main barrel as the main component part of the objective. The main barrel contains at least one correction mount, cylinder sleeves, mounting rings, carrier rings and/or adjusting rings, and imaging optical elements. In order to control the temperature, at least one structural component part of the objective or an element arranged between structural component parts of the objective is constructed as a temperature-controllable element or as a temperature-controllable foil which is connected (not shown) by leads to a device for monitoring temperature. Further, a temperature gauge or temperature sensor is arranged in the objective and is likewise connected to the device for monitoring temperature.

摘要翻译： 本发明涉及温度可控的目标，特别是对于显微镜和其它光学设备，其包括主镜筒作为物镜的主要组成部分。 主桶包含至少一个校正座，气缸套，安装环，承载环和/或调节环以及成像光学元件。 为了控制温度，物镜的至少一个结构部件或布置在物镜的结构部件之间的元件被构造为温度可控元件或作为温度可控箔片，其被连接（未示出）由 导致监测温度的设备。 此外，温度计或温度传感器布置在物镜中，并且同样地连接到用于监测温度的装置。