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    Process for preparing an antipoliomyelitis vaccine
    1.
    发明授权
    Process for preparing an antipoliomyelitis vaccine 失效
    抗脊髓灰质炎疫苗的制备方法

    公开(公告)号：US3197372A

    公开(公告)日：1965-07-27

    申请号：US22948262

    申请日：1962-10-09

    申请人： BEHRINGWERKE AG

    发明人： WERNER SCHAFER ,  HEINZ RESTLE

    IPC分类号： A61K39/13

    CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/13 ,  A61K2039/5252 ,  C12N2770/32634 ,  C12N2770/32663

    摘要： An antipoliomyelitis vaccine is prepared by inactivating the poliomyelitis virus by treatment with hydroxylamine in a concentration of 0.01 to 5.0 mols/litre and (a) a pH of 4.5 to 7.0 and preferably 6.0 and temperature of 4 DEG to 40 DEG C. or (b) a pH of 6.5 to 7.0 and preferably 7.0 and a temperature of 30 DEG to 40 DEG C. and preferably 37 DEG C., allowing the hydroxylamine to act on the viruses, which may be contaminated with Simian virus 40, until the latter is free from infectious viruses, binding unreacted hydroxylamine with a carbonyl compound capable of forming an oxime therewith, and processing the inactivated virus into a vaccine in known manner. Prior to the inactivation step, the virus suspension may be concentrated. The oxime, if soluble, may be removed by dialysis and, if insoluble, it may be removed by filtration or centrifugal action. Specified carbonyl compounds are acetone, acetylacetone, acetonyl acetone, acetoacetic acid ethyl ester or acetoacetic acid methyl ester. Preferably 1-10 times the stoichiometrical amount of carbonyl compound, calculated on the hydroxylamine used, may be used for binding unreacted hydroxylamine. Conventional adjuvants for increasing antibody formation, e.g. aluminium hydroxide or phosphate, may be present.

    Method for inactivating non-enveloped viruses using a viricide-potentiating agent with a photoactivatible virucide
    7.
    发明授权
    Method for inactivating non-enveloped viruses using a viricide-potentiating agent with a photoactivatible virucide 失效
    使用杀病毒剂增强剂与光活化型病毒杀灭剂灭活非包膜病毒的方法

    公开(公告)号：US5663043A

    公开(公告)日：1997-09-02

    申请号：US368780

    申请日：1995-01-04

    申请人： Charles M. Zepp ,  Donald L. Heefner

    发明人： Charles M. Zepp ,  Donald L. Heefner

    IPC分类号： A61L2/00 ,  C12N7/06 ,  A01N1/02

    CPC分类号： C12N7/00 ,  A61L2/0011 ,  A61L2/0088 ,  C12N2710/10363 ,  C12N2750/14363 ,  C12N2770/32463 ,  C12N2770/32663

    摘要： Method for inactivating non-enveloped viruses using a viricide-potentiating agent. In a preferred embodiment, the method may be used to inactivate non-enveloped viruses present within a sample of whole blood or a blood product and comprises (a) adding to the blood sample a photoactivatable viricide, such as a psoralen, hypericin, methylene blue, toluidine blue or the like, which, when activated, is effective in inactivating enveloped viruses; (b) adding to the blood sample a viricide-potentiating chemical agent that increases the sensitivity of non-enveloped viruses to the activated viricide; and (c) activating the photoactivatable viricide. Preferably, the viricide-potentiating chemical agent includes a first moiety which possesses an affinity for a component of the non-enveloped virus and a second moiety which includes a lipid tail, the first and second moieties being structurally interrelated so that, when the first moiety becomes associated with a component of the non-enveloped virus, the second moiety penetrates or at least partially surrounds the viral capsid of the non-enveloped virus. Examples of the chemical agent include cationic lipopolyamines, such as dioctadecylamidoglycylspermine.

    摘要翻译： 使用杀病毒剂增效剂灭活非包膜病毒的方法。 在优选的实施方案中，该方法可用于灭活全血或血液制品样品中存在的非包膜病毒，并且包括（a）向血液样品中加入可光活化的杀戮剂，例如补骨脂素，金丝桃素，亚甲基蓝 甲苯胺蓝等，其在活化时有效灭活包膜病毒; （b）向血液样品中加入增加非包膜病毒对活化杀戮剂的敏感性的杀病剂增效化学试剂; 和（c）活化可光活化杀戮剂。 优选地，杀病毒剂增强化学试剂包括对非包膜病毒的组分具有亲和力的第一部分和包含脂质尾部的第二部分，第一和第二部分在结构上相互关联，使得当第一部分 变得与非包膜病毒的组分相关联，第二部分渗透或至少部分地包围非包膜病毒的病毒衣壳。 化学试剂的实例包括阳离子脂多胺，如双十八烷基酰氨基甘氨酰精胺。