公开(公告)号：US12104160B2

公开(公告)日：2024-10-01

申请号：US17307850

申请日：2021-05-04

申请人： ZYMOCHEM, INC.

发明人： Harshal Chokhawala

IPC分类号： C12P7/42 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/24 ,  C12N9/88 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/44 ,  C12P7/6409 ,  C12P13/00 ,  C12P17/08 ,  C12P17/10

CPC分类号： C12N15/52 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1205 ,  C12N9/13 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/88 ,  C12N9/93 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/6409 ,  C12P13/001 ,  C12P13/005 ,  C12P17/08 ,  C12P17/10 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01035 ,  C12Y101/01078 ,  C12Y101/01268 ,  C12Y101/01269 ,  C12Y102/01003 ,  C12Y102/01005 ,  C12Y102/01022 ,  C12Y102/01024 ,  C12Y102/01026 ,  C12Y102/01048 ,  C12Y102/01063 ,  C12Y102/07005 ,  C12Y102/99006 ,  C12Y103/01044 ,  C12Y103/01045 ,  C12Y103/01086 ,  C12Y104/01 ,  C12Y203/01001 ,  C12Y203/01032 ,  C12Y203/01035 ,  C12Y203/01057 ,  C12Y206/01008 ,  C12Y206/01009 ,  C12Y206/01036 ,  C12Y206/01043 ,  C12Y206/01048 ,  C12Y206/01076 ,  C12Y206/01082 ,  C12Y207/01031 ,  C12Y207/01165 ,  C12Y208/03 ,  C12Y301/03 ,  C12Y301/03002 ,  C12Y301/03008 ,  C12Y301/03019 ,  C12Y301/0302 ,  C12Y302/01 ,  C12Y401/01001 ,  C12Y401/02 ,  C12Y401/02014 ,  C12Y401/0202 ,  C12Y401/02021 ,  C12Y401/03016 ,  C12Y401/03017 ,  C12Y402/01002 ,  C12Y402/01003 ,  C12Y402/01028 ,  C12Y402/0103 ,  C12Y402/01079 ,  C12Y402/0112 ,  C12Y602/01 ,  Y02E50/30

摘要： Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a CN aldehyde and pyruvate to a CN+3 β-hydroxyketone intermediate through an aldol addition; and b) converting the CN+3 β-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps.

公开(公告)号：US20170044551A1

公开(公告)日：2017-02-16

申请号：US15072140

申请日：2016-03-16

申请人： ZYMOCHEM INC.

发明人： Harshal Chokhawala

IPC分类号： C12N15/52 ,  C12N9/16 ,  C12N9/12 ,  C12N9/88 ,  C12P7/24 ,  C12N9/02 ,  C12P7/40 ,  C12P7/44 ,  C12P13/00 ,  C12P17/10 ,  C12P7/18 ,  C12P7/04 ,  C12P7/64 ,  C12N9/04 ,  C12N9/10 ,  C12N9/00 ,  C12N9/24 ,  C12N9/06 ,  C12P7/42

CPC分类号： C12N15/52 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1205 ,  C12N9/13 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/88 ,  C12N9/93 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/6409 ,  C12P13/001 ,  C12P13/005 ,  C12P17/08 ,  C12P17/10 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01035 ,  C12Y101/01078 ,  C12Y101/01268 ,  C12Y101/01269 ,  C12Y102/01003 ,  C12Y102/01005 ,  C12Y102/01022 ,  C12Y102/01024 ,  C12Y102/01026 ,  C12Y102/01048 ,  C12Y102/01063 ,  C12Y102/07005 ,  C12Y102/99006 ,  C12Y103/01044 ,  C12Y103/01045 ,  C12Y103/01086 ,  C12Y104/01 ,  C12Y203/01001 ,  C12Y203/01032 ,  C12Y203/01035 ,  C12Y203/01057 ,  C12Y206/01008 ,  C12Y206/01009 ,  C12Y206/01036 ,  C12Y206/01043 ,  C12Y206/01048 ,  C12Y206/01076 ,  C12Y206/01082 ,  C12Y207/01031 ,  C12Y207/01165 ,  C12Y208/03 ,  C12Y301/03 ,  C12Y301/03002 ,  C12Y301/03008 ,  C12Y301/03019 ,  C12Y301/0302 ,  C12Y302/01 ,  C12Y401/01001 ,  C12Y401/02 ,  C12Y401/02014 ,  C12Y401/0202 ,  C12Y401/02021 ,  C12Y401/03016 ,  C12Y401/03017 ,  C12Y402/01002 ,  C12Y402/01003 ,  C12Y402/01028 ,  C12Y402/0103 ,  C12Y402/01079 ,  C12Y402/0112 ,  C12Y602/01 ,  Y02E50/343

摘要： Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as 1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a CN aldehyde and pyruvate to a CN+3 β-hydroxyketone intermediate through an aldol addition; and b) converting the CN+3β-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps.

摘要翻译： 本文提供了用于制备化合物如1-丁醇，丁酸，琥珀酸，1,4-丁二醇，1-戊醇，戊酸，戊二酸，1,5-戊二醇的方法，组合物和非天然存在的微生物生物 ，1-己醇，己酸，己二酸，1,6-己二醇，6-羟基己酸，ε-己内酯，6-氨基 - 己酸，ε-己内酰胺，六亚甲基二胺，线性脂肪酸和线性脂肪醇， 7-25碳原子长度为6-24个碳原子的直链烷烃和直链α-烯烃，癸二酸和十二烷二酸包括：a）通过醛醇加成将CN醛和丙酮酸盐转化为CN +3β-羟基酮中间体 ; 和b）通过酶促步骤或酶和化学步骤的组合将CN +3β-羟基酮中间体转化为化合物。