公开(公告)号：US20180179556A1

公开(公告)日：2018-06-28

申请号：US15598316

申请日：2017-05-18

申请人： National Tsing Hua University ,  Chang Chun Plastics Co., Ltd. ,  Chang Chun Petrochemical Co., Ltd.

发明人： Roa-Pu Shen ,  Rex C. Wen

IPC分类号： C12P7/16 ,  C12N9/04 ,  C12N9/02 ,  C12N9/12 ,  C12N9/10 ,  C12N15/70 ,  C12N15/52

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1217 ,  C12N15/52 ,  C12N15/70 ,  C12N2800/101 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y101/0103 ,  C12Y101/01322 ,  C12Y102/01004 ,  C12Y103/01086 ,  C12Y103/05004 ,  C12Y203/01009 ,  C12Y207/02001

摘要： A butanol expression cassette includes a butanol production related genes and a fermentation regulatory element. The fermentation regulatory element controls the expression of the butanol production related gene and locates upstream of the butanol production related gene. The fermentation regulatory element includes a promoter, a ribosome binding site and a transcription factor binding site of a fermentation gene. A fermentation in which the fermentation regulatory element involves includes an acetic acid fermentation, an alcohol fermentation, a succinic acid fermentation or a lactic acid fermentation, the butanol production related gene is not the fermentation gene or a gene of an upstream product of the fermentation in which the fermentation gene involves. The present invention provides a recombinant plasmid formed by cloning the butanol expression cassettes in the expression vector. The present invention also provides a butanol production related gene expression method to express butanol production related gene by using recombinant plasmid.

公开(公告)号：US20230242947A1

公开(公告)日：2023-08-03

申请号：US18046695

申请日：2022-10-14

申请人： Arpita Bose ,  Wei Bai

发明人： Arpita Bose ,  Wei Bai

IPC分类号： C12P7/625 ,  C12N15/52 ,  C12N15/74 ,  C12N9/12

CPC分类号： C12P7/625 ,  C12N15/52 ,  C12N15/74 ,  C12N9/1241 ,  C12Y401/01039 ,  C12Y402/01027 ,  C12Y103/01086 ,  C12Y102/01057 ,  C12Y101/05 ,  C12N2800/101 ,  C12N2820/002

摘要： Among the various aspects of the present disclosure is the provision of a genetically engineered transgenic microorganisms Rhodopseudomonas palustris and methods of making and using the same.

公开(公告)号：US09777297B2

公开(公告)日：2017-10-03

申请号：US14356386

申请日：2012-11-02

申请人： Kwang Myung Cho ,  Wendy Higashide ,  Chrissie Lee ,  Shahrooz Rabizadeh

发明人： Kwang Myung Cho ,  Wendy Higashide ,  Chrissie Lee ,  Shahrooz Rabizadeh

IPC分类号： C12P7/16 ,  C12P7/24 ,  C12N15/52 ,  C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/24 ,  C12Y101/01157 ,  C12Y102/01057 ,  C12Y103/01086 ,  C12Y203/01009

摘要： Microorganisms and methods of producing n-butyraldehyde with enhanced yields are presented in which a microorganism is engineered to enhance the conversion of a carbon source into n-butyraldehyde. The n-butyraldehyde is recovered by way of a gas stripping process that occurs during the conversion process, providing significantly greater product yield than post-fermentation recovery of n-butyraldehyde alone.

公开(公告)号：US20180010155A1

公开(公告)日：2018-01-11

申请号：US15697979

申请日：2017-09-07

申请人： Easel Biotechnologies, LLC

发明人： Kwang Myung Cho ,  Wendy Higashide ,  Chrissie Lee ,  Shahrooz Rabizadeh

IPC分类号： C12P7/16 ,  C12N15/52 ,  C12N9/10 ,  C12N9/04 ,  C12P7/24 ,  C12N9/02

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/24 ,  C12Y101/01157 ,  C12Y102/01057 ,  C12Y103/01086 ,  C12Y203/01009

摘要： Microorganisms and methods of producing n-butyraldehyde with enhanced yields are presented in which a microorganism is engineered to enhance the conversion of a carbon source into n-butyraldehyde. The n-butyraldehyde is recovered by way of a gas stripping process that occurs during the conversion process, providing significantly greater product yield than post-fermentation recovery of n-butyraldehyde alone.

公开(公告)号：US20140004597A1

公开(公告)日：2014-01-02

申请号：US13708824

申请日：2012-12-07

申请人： The Regents of the University of California

发明人： Michelle C.Y. CHANG ,  Brooks Bond-Watts ,  Miao Wen ,  Jeffrey A. Hanson

IPC分类号： C12P7/16

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/52 ,  C12N15/70 ,  C12N15/81 ,  C12P2203/00 ,  C12Y101/01001 ,  C12Y101/01036 ,  C12Y102/07001 ,  C12Y103/01086 ,  C12Y203/01016 ,  C12Y402/01017 ,  Y02E50/10

摘要： The present disclosure provides optimized recombinant cells for the production of n-butanol. Methods for the use of these cells are also provided. Specifically, the utility of acylating aldehyde dehydrogenases and pyruvate:flavodoxin/ferredoxin-oxidoreductase for the improvement of n-butanol yields from recombinant cells is disclosed.

摘要翻译： 本公开提供用于生产正丁醇的优化的重组细胞。 还提供了使用这些电池的方法。 具体地，公开了用于改善醛脱氢酶和丙酮酸：黄素氧还蛋白/铁氧还蛋白 - 氧化还原酶以改善重组细胞的正丁醇产率的效用。

公开(公告)号：US12104160B2

公开(公告)日：2024-10-01

申请号：US17307850

申请日：2021-05-04

申请人： ZYMOCHEM, INC.

发明人： Harshal Chokhawala

IPC分类号： C12P7/42 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/24 ,  C12N9/88 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/44 ,  C12P7/6409 ,  C12P13/00 ,  C12P17/08 ,  C12P17/10

CPC分类号： C12N15/52 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1205 ,  C12N9/13 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/88 ,  C12N9/93 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/6409 ,  C12P13/001 ,  C12P13/005 ,  C12P17/08 ,  C12P17/10 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01035 ,  C12Y101/01078 ,  C12Y101/01268 ,  C12Y101/01269 ,  C12Y102/01003 ,  C12Y102/01005 ,  C12Y102/01022 ,  C12Y102/01024 ,  C12Y102/01026 ,  C12Y102/01048 ,  C12Y102/01063 ,  C12Y102/07005 ,  C12Y102/99006 ,  C12Y103/01044 ,  C12Y103/01045 ,  C12Y103/01086 ,  C12Y104/01 ,  C12Y203/01001 ,  C12Y203/01032 ,  C12Y203/01035 ,  C12Y203/01057 ,  C12Y206/01008 ,  C12Y206/01009 ,  C12Y206/01036 ,  C12Y206/01043 ,  C12Y206/01048 ,  C12Y206/01076 ,  C12Y206/01082 ,  C12Y207/01031 ,  C12Y207/01165 ,  C12Y208/03 ,  C12Y301/03 ,  C12Y301/03002 ,  C12Y301/03008 ,  C12Y301/03019 ,  C12Y301/0302 ,  C12Y302/01 ,  C12Y401/01001 ,  C12Y401/02 ,  C12Y401/02014 ,  C12Y401/0202 ,  C12Y401/02021 ,  C12Y401/03016 ,  C12Y401/03017 ,  C12Y402/01002 ,  C12Y402/01003 ,  C12Y402/01028 ,  C12Y402/0103 ,  C12Y402/01079 ,  C12Y402/0112 ,  C12Y602/01 ,  Y02E50/30

摘要： Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a CN aldehyde and pyruvate to a CN+3 β-hydroxyketone intermediate through an aldol addition; and b) converting the CN+3 β-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps.

公开(公告)号：US20170044551A1

公开(公告)日：2017-02-16

申请号：US15072140

申请日：2016-03-16

申请人： ZYMOCHEM INC.

发明人： Harshal Chokhawala

IPC分类号： C12N15/52 ,  C12N9/16 ,  C12N9/12 ,  C12N9/88 ,  C12P7/24 ,  C12N9/02 ,  C12P7/40 ,  C12P7/44 ,  C12P13/00 ,  C12P17/10 ,  C12P7/18 ,  C12P7/04 ,  C12P7/64 ,  C12N9/04 ,  C12N9/10 ,  C12N9/00 ,  C12N9/24 ,  C12N9/06 ,  C12P7/42

CPC分类号： C12N15/52 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1205 ,  C12N9/13 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/88 ,  C12N9/93 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/6409 ,  C12P13/001 ,  C12P13/005 ,  C12P17/08 ,  C12P17/10 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01035 ,  C12Y101/01078 ,  C12Y101/01268 ,  C12Y101/01269 ,  C12Y102/01003 ,  C12Y102/01005 ,  C12Y102/01022 ,  C12Y102/01024 ,  C12Y102/01026 ,  C12Y102/01048 ,  C12Y102/01063 ,  C12Y102/07005 ,  C12Y102/99006 ,  C12Y103/01044 ,  C12Y103/01045 ,  C12Y103/01086 ,  C12Y104/01 ,  C12Y203/01001 ,  C12Y203/01032 ,  C12Y203/01035 ,  C12Y203/01057 ,  C12Y206/01008 ,  C12Y206/01009 ,  C12Y206/01036 ,  C12Y206/01043 ,  C12Y206/01048 ,  C12Y206/01076 ,  C12Y206/01082 ,  C12Y207/01031 ,  C12Y207/01165 ,  C12Y208/03 ,  C12Y301/03 ,  C12Y301/03002 ,  C12Y301/03008 ,  C12Y301/03019 ,  C12Y301/0302 ,  C12Y302/01 ,  C12Y401/01001 ,  C12Y401/02 ,  C12Y401/02014 ,  C12Y401/0202 ,  C12Y401/02021 ,  C12Y401/03016 ,  C12Y401/03017 ,  C12Y402/01002 ,  C12Y402/01003 ,  C12Y402/01028 ,  C12Y402/0103 ,  C12Y402/01079 ,  C12Y402/0112 ,  C12Y602/01 ,  Y02E50/343

摘要： Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as 1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a CN aldehyde and pyruvate to a CN+3 β-hydroxyketone intermediate through an aldol addition; and b) converting the CN+3β-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps.

摘要翻译： 本文提供了用于制备化合物如1-丁醇，丁酸，琥珀酸，1,4-丁二醇，1-戊醇，戊酸，戊二酸，1,5-戊二醇的方法，组合物和非天然存在的微生物生物 ，1-己醇，己酸，己二酸，1,6-己二醇，6-羟基己酸，ε-己内酯，6-氨基 - 己酸，ε-己内酰胺，六亚甲基二胺，线性脂肪酸和线性脂肪醇， 7-25碳原子长度为6-24个碳原子的直链烷烃和直链α-烯烃，癸二酸和十二烷二酸包括：a）通过醛醇加成将CN醛和丙酮酸盐转化为CN +3β-羟基酮中间体 ; 和b）通过酶促步骤或酶和化学步骤的组合将CN +3β-羟基酮中间体转化为化合物。

公开(公告)号：US20140322774A1

公开(公告)日：2014-10-30

申请号：US14356386

申请日：2012-11-02

申请人： Easel Biotechnologies, LLC

发明人： Kwang Myung Cho ,  Wendy Higashide ,  Chrissie Lee ,  Shahrooz Rabizadeh

IPC分类号： C12P7/24 ,  C12P7/16

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/24 ,  C12Y101/01157 ,  C12Y102/01057 ,  C12Y103/01086 ,  C12Y203/01009

摘要： Microorganisms and methods of producing n-butyraldehyde with enhanced yields are presented in which a microorganism is engineered to enhance the conversion of a carbon source into n-butyraldehyde. The n-butyraldehyde is recovered by way of a gas stripping process that occurs during the conversion process, providing significantly greater product yield than post-fermentation recovery of n-butyraldehyde alone.

摘要翻译： 介绍了微生物和生产具有提高的产量的正丁醛的方法，其中微生物被工程化以增强碳源转化为正丁醛。 正丁醛通过在转化过程中发生的气体剥离方法回收，与单独的正丁醛的发酵后回收相比提供了显着更大的产物产率。