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    ENZYMATIC CONVERSION OF BOTH ENANTIOMERS OF 1,2-PROPANEDIOL TO PROPIONALDEHYDE
    1.
    发明申请
    ENZYMATIC CONVERSION OF BOTH ENANTIOMERS OF 1,2-PROPANEDIOL TO PROPIONALDEHYDE 审中-公开
    1,2-丙二醇对丙二醛的两种促进剂的酶转化

    公开(公告)号：US20160040196A1

    公开(公告)日：2016-02-11

    申请号：US14823030

    申请日：2015-08-11

    申请人： University of Georgia Research Foundation, Inc.

    发明人： William Lanzilotta

    IPC分类号： C12P7/24 ,  C12N9/88 ,  C12N9/04

    CPC分类号： C12P7/24 ,  C12N9/88 ,  C12P7/04 ,  C12Y101/01001 ,  C12Y402/01028

    摘要： Engineered organisms, cell-free enzyme mixtures, and methods are provided for converting both enantiomers of 1,2-propanediol to propionaldehyde. Engineered organisms are provided that convert both enantiomers of 1,2-propanediol to propionaldehyde but do not convert glycerol to 3-hydroxypropionaldehyde and/or do not convert propanal to propanol. The engineered organisms and cell-free enzyme mixtures can contain a diol dehydratase enzyme similar in sequence identity to Roseburia inulinivorans diol dehydratase. The engineered organisms and cell-free enzyme mixtures can contain a diol dehydratase activating enzyme similar in sequence identity to Roseburia inulinivorans diol dehydratase activating enzyme. Methods of converting both enantiomers of 1-2-propanediol to propanol can include culturing a microorganism provided herein under conditions and for a period of time sufficient to convert the 1,2-propanediol to propanol. The conditions can include a substantially anaerobic culture medium.

    摘要翻译： 提供了工程生物，无细胞的酶混合物和方法，用于将1,2-丙二醇的对映异构体转化为丙醛。 提供了将1,2-丙二醇的对映异构体转化为丙醛但不将甘油转化为3-羟基丙醛和/或不将丙醛转化为丙醇的工程生物。 工程生物体和无细胞的酶混合物可以含有与玫瑰红霉素二甘醇脱水酶相似的二醇脱水酶。 工程生物和无细胞的酶混合物可以含有与玫瑰红霉素二氢脱水酶活化酶的玫瑰红的序列同一性的二醇脱水酶活化酶。 将1-2丙二醇的对映异构体转化为丙醇的方法可以包括在足以将1,2-丙二醇转化为丙醇的条件下和一段时间内培养本文提供的微生物。 条件可以包括基本上厌氧的培养基。

    Recombinant microorganisms and uses therefor
    2.
    发明申请
    Recombinant microorganisms and uses therefor 有权
    重组微生物及其用途

    公开(公告)号：US20130330809A1

    公开(公告)日：2013-12-12

    申请号：US13914234

    申请日：2013-06-10

    申请人： LanzaTech New Zealand Limited

    发明人： Alexander Paul Mueller ,  Michael Koepke ,  Shilpa Nagaraju

    IPC分类号： C12N15/74

    CPC分类号： C12N15/74 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/88 ,  C12P7/16 ,  C12Y101/01004 ,  C12Y402/01028 ,  C12Y402/0103 ,  Y02E50/10

    摘要： Carboxydotrophic acetogenic microorganisms do not produce MEK and/or 2-butanol. They lack the biosynthesis pathways to make these products. In addition, they produce the intermediate (R,R)-2,3-butanediol whereas the production of MEK and 2-butanol requires production of the intermediate (R,S)-2,3-butanediol. Nonetheless, the production of MEK and/or 2-butanol can be accomplished using recombinant microorganisms adapted to express or overexpress key enzymes in the MEK and/or 2-butanol biosynthesis pathways. Such microorganisms, such as the carboxydotrophic acetogen Clostridium autoethanogenum, can ferment substrates comprising CO. The overall scheme involves the production of 2-butanol from (R,S)-2,3-butanediol and the conversion of (R)-acetoin to (S)-2,3-butanediol. These steps are involved in the production of both MEK and 2-butanol. Such fermentation methods offer a means of using carbon monoxide from industrial processes which would otherwise be released into the atmosphere and pollute the environment.

    摘要翻译： 羧化营养性致病微生物不产生MEK和/或2-丁醇。 他们缺乏制作这些产品的生物合成途径。 此外，它们生产中间体（R，R）-2,3-丁二醇，而生产MEK和2-丁醇需要生产中间体（R，S）-2,3-丁二醇。 然而，可以使用适于在MEK和/或2-丁醇生物合成途径中表达或过表达关键酶的重组微生物来实现MEK和/或2-丁醇的生产。 这种微生物，如羧基营养型乙酰胆碱梭菌，它可以发酵包含CO的底物。总体方案包括从（R，S）-2,3-丁二醇生产2-丁醇和（R） - 乙酰胆碱转化为（ S）-2,3-丁二醇。 这些步骤涉及MEK和2-丁醇的生产。 这种发酵方法提供了使用工业过程中的一氧化碳的方法，否则这些方法将被释放到大气中并污染环境。

    Recombinant microorganisms and uses therefor
    7.
    发明授权
    Recombinant microorganisms and uses therefor 有权

    公开(公告)号：US09890384B2

    公开(公告)日：2018-02-13

    申请号：US13914234

    申请日：2013-06-10

    申请人： LanzaTech New Zealand Limited

    发明人： Alexander Paul Mueller ,  Michael Koepke ,  Shilpa Nagaraju

    IPC分类号： C12N1/20 ,  C12N9/04 ,  C12N9/88 ,  C12N15/74 ,  C12P7/16

    CPC分类号： C12N15/74 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/88 ,  C12P7/16 ,  C12Y101/01004 ,  C12Y402/01028 ,  C12Y402/0103 ,  Y02E50/10

    摘要： Carboxydotrophic acetogenic microorganisms do not produce MEK and/or 2-butanol. They lack the biosynthesis pathways to make these products. In addition, they produce the intermediate (R,R)-2,3-butanediol whereas the production of MEK and 2-butanol requires production of the intermediate (R,S)-2,3-butanediol. Nonetheless, the production of MEK and/or 2-butanol can be accomplished using recombinant microorganisms adapted to express or overexpress key enzymes in the MEK and/or 2-butanol biosynthesis pathways. Such microorganisms, such as the carboxydotrophic acetogen Clostridium autoethanogenum, can ferment substrates comprising CO. The overall scheme involves the production of 2-butanol from (R,S)-2,3-butanediol and the conversion of (R)-acetoin to (S)-2,3-butanediol. These steps are involved in the production of both MEK and 2-butanol. Such fermentation methods offer a means of using carbon monoxide from industrial processes which would otherwise be released into the atmosphere and pollute the environment.

    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF KETONES
    8.
    发明申请
    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF KETONES 审中-公开
    用于生产酮的微生物和方法

    公开(公告)号：US20150152445A1

    公开(公告)日：2015-06-04

    申请号：US14559881

    申请日：2014-12-03

    申请人： LanzaTech New Zealand Limited

    发明人： Michael Koepke ,  Bakir Al-Sinawi ,  Rasmus Overgaard Jensen ,  Alexander Paul Mueller ,  Ryan Edward Hill

    IPC分类号： C12P7/28 ,  C12N9/88 ,  C12P7/26 ,  C12N9/10

    CPC分类号： C12P7/28 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/88 ,  C12P7/26 ,  C12Y208/03 ,  C12Y401/01004 ,  C12Y402/01028

    摘要： The invention provides a recombinant, acetogenic, carboxydotrophic bacterium that lacks secondary alcohol dehydrogenase or comprises an inactivated secondary alcohol dehydrogenase. The inactivated secondary alcohol dehydrogenase may be encoded by a secondary alcohol dehydrogenase gene comprising an inactivating mutation that reduces the ability of the bacterium to convert acetone to isopropanol and to convert methyl ethyl ketone to 2-butanol. Since a bacterium that lacks secondary alcohol dehydrogenase or comprises an inactivated secondary alcohol dehydrogenase accumulates carbonyl-containing compounds, the invention also provides a method of producing carbonyl-containing compounds, such as acetone and methyl ethyl ketone.

    摘要翻译： 本发明提供了缺乏仲醇脱氢酶或包含失活的仲醇脱氢酶的重组，乙酰酵素，营养不良的细菌。 灭活的仲醇脱氢酶可以由包含失活突变的仲醇脱氢酶基因编码，所述失活突变降低细菌将丙酮转化为异丙醇并将甲基乙基酮转化为2-丁醇的能力。 由于缺乏仲醇脱氢酶或包含失活的仲醇脱氢酶的细菌积累含羰基化合物，本发明还提供了生产含羰基化合物如丙酮和甲基乙基酮的方法。

    Recombinant microorganisms and uses therefor
    9.
    发明申请
    Recombinant microorganisms and uses therefor 有权
    重组微生物及其用途

    公开(公告)号：US20140065698A1

    公开(公告)日：2014-03-06

    申请号：US14011872

    申请日：2013-08-28

    申请人： LanzaTech New Zealand Limited

    发明人： Alexander Paul Mueller ,  Michael Koepke

    IPC分类号： C12N15/70

    CPC分类号： C12N15/70 ,  C12N9/88 ,  C12P7/04 ,  C12P7/24 ,  C12P7/28 ,  C12P41/002 ,  C12Y402/01028

    摘要： A stereospecific enzyme in C. autoethanogenum permits the conversion of racemic propanediol to acetone and/or propionaldehyde. Entantiomeric starting materials lead to different products. If desired, the products may be reduced to form alcohols. The reaction can be performed in various host cells, so that various materials may be used as carbon and/or energy sources.

    摘要翻译： C.etetethanogenum中的立体特异性酶允许外消旋丙二醇转化为丙酮和/或丙醛。 对映异构起始材料导致不同的产品。 如果需要，可以将产物还原成醇。 反应可以在各种宿主细胞中进行，使得各种材料可以用作碳和/或能源。