公开(公告)号：US07498128B2

公开(公告)日：2009-03-03

申请号：US11296829

申请日：2005-12-06

Applicant: Steven M. Watkins

Inventor： Steven M. Watkins

IPC: C12Q1/00

CPC classification number: G01N33/92 ,  A61K31/00 ,  A61K31/4415 ,  A61K31/455 ,  A61K31/51 ,  A61K31/519 ,  A61K31/7076 ,  A61K38/27 ,  C12N9/1003 ,  C12Y201/01017 ,  G01N33/57484 ,  G01N2800/52

Abstract: The present invention provides methods for assessing contribution of one or more pathways to the biosynthesis of a metabolite, e.g., a lipid using the levels of the product made and the metabolite precursors for each pathway.

Abstract translation: 本发明提供用于评估一种或多种途径对代谢产物（例如使用制备的产物的水平和每个途径的代谢前体）的生物合成的贡献的方法。

公开(公告)号：US20060141550A1

公开(公告)日：2006-06-29

申请号：US11291272

申请日：2005-11-30

Applicant: Steven Watkins ,  Rebecca Baillie

Inventor： Steven Watkins ,  Rebecca Baillie

IPC: C12Q1/48 ,  A61K38/45

CPC classification number: G01N33/92 ,  A61K31/00 ,  A61K31/4415 ,  A61K31/455 ,  A61K31/51 ,  A61K31/519 ,  A61K31/7076 ,  A61K38/27 ,  C12N9/1003 ,  C12Y201/01017 ,  G01N33/57484 ,  G01N2800/52

Abstract: The present invention provides metabolic markers and targets, especially PEMT activity and metabolic markers and components associated therewith useful for diagnosing and treating various disease conditions.

Abstract translation: 本发明提供代谢标志物和靶标，特别是PEMT活性和与其相关的代谢标志物和组分，其可用于诊断和治疗各种疾病状况。

公开(公告)号：US20230220326A1

公开(公告)日：2023-07-13

申请号：US17796565

申请日：2021-03-05

Applicant: CJ CHEILJEDANG CORPORATION

Inventor： Jiryang JANG ,  Peter LEE ,  Jee Yeon BAE ,  Ju-yeon KIM ,  Hye Min PARK ,  Hyung Joon KIM ,  Sang Min PARK

IPC: C12N1/16 ,  A61K8/9728 ,  A23K10/16 ,  A23K20/158 ,  A61K36/06

CPC classification number: C12N1/16 ,  A61K8/9728 ,  A23K10/16 ,  A23K20/158 ,  A61K36/06 ,  C12Y201/01017

Abstract: Provided is a variant strain of the genus Yarrowia. More specifically, provided are a variant strain of the genus Yarrowia, in which activity of phosphatidylethanolamine N-methyltransferase (PEMT) or phospholipid methyltransferase is inactivated, and a method of increasing a fat in the strain, including culturing the strain, or a method of preparing a fat.

公开(公告)号：US20040024065A1

公开(公告)日：2004-02-05

申请号：US10383850

申请日：2003-03-07

Inventor： Steven M. Watkins ,  Rebecca A. Baillie

IPC: A61K031/202

CPC classification number: G01N33/92 ,  A61K31/00 ,  A61K31/4415 ,  A61K31/455 ,  A61K31/51 ,  A61K31/519 ,  A61K31/7076 ,  A61K38/27 ,  C12N9/1003 ,  C12Y201/01017 ,  G01N33/57484 ,  G01N2800/52

Abstract: The present invention provides metabolic markers and targets, especially PEMT activity and metabolic markers and components associated therewith useful for diagnosing and treating various disease conditions.

Abstract translation: 本发明提供代谢标志物和靶标，特别是PEMT活性和与其相关的代谢标志物和组分，其可用于诊断和治疗各种疾病状况。

公开(公告)号：US06391604B1

公开(公告)日：2002-05-21

申请号：US09519729

申请日：2000-03-03

Applicant: Ikuo Matsui ,  Kazuhiko Ishikawa ,  Hiroyasu Ishida ,  Yoshitsugu Kosugi ,  Yasutaka Tahara

Inventor： Ikuo Matsui ,  Kazuhiko Ishikawa ,  Hiroyasu Ishida ,  Yoshitsugu Kosugi ,  Yasutaka Tahara

IPC: C12N910

CPC classification number: C12N9/1007 ,  C12Y201/01017 ,  Y10S435/822

Abstract: A thermostable phosphatidylethanolamine N-methyltransferase and a process for producing the enzyme are provided. A host cell is transformed with an expression vector containing DNA encoding a thermostable enzyme derived from hyper-thermophilic archaea such as Pyrococcus, the enzyme having phosphatidylethanolamine N-methyltransferase activity and an optimum temperature of 90° C. or higher, and the transformed host cell is cultured.

Abstract translation: 提供了热稳定性磷脂酰乙醇胺N-甲基转移酶及其制备方法。 宿主细胞用含有编码来源于嗜热古细菌的热稳定酶的DNA的表达载体转化，如Pyrococcus，具有磷脂酰乙醇胺N-甲基转移酶活性的酶，最适温度为90℃或更高，转化的宿主细胞 被培养

公开(公告)号：US20140322771A1

公开(公告)日：2014-10-30

申请号：US13992444

申请日：2011-12-09

Applicant: (Robert) Hongyuan Yang ,  Junmin Pan ,  Jacqueline Batley

Inventor： (Robert) Hongyuan Yang ,  Junmin Pan ,  Jacqueline Batley

IPC: C12P7/64 ,  C12N15/63

CPC classification number: C12P7/649 ,  C12N9/1007 ,  C12N15/63 ,  C12N15/8243 ,  C12N15/8247 ,  C12P7/6445 ,  C12P7/6463 ,  C12Y201/01017

Abstract: The invention relates to methods for producing lipids and in particular methods for producing glycerolipids suitable for use in generating biofuels. The glycerolipids are produced through the modification of a cell so as to increase phosphatidic acid via the inhibition of the phosphatidylethanolamine N-methyltransferase (PEMT) pathway. The invention further relates to genetically modified plants and microorganisms in which the production of glycerolipids/oil is increased.

Abstract translation: 本发明涉及用于生产脂质的方法，特别涉及生产适用于产生生物燃料的甘油脂的方法。 通过改变细胞产生甘油脂，以通过抑制磷脂酰乙醇胺N-甲基转移酶（PEMT）途径来增加磷脂酸。 本发明还涉及增加甘油脂/油的生产的转基因植物和微生物。

公开(公告)号：US20060084129A1

公开(公告)日：2006-04-20

申请号：US11296829

申请日：2005-12-06

Applicant: Steven Watkins

Inventor： Steven Watkins

IPC: C12Q1/48 ,  G01N33/92

CPC classification number: G01N33/92 ,  A61K31/00 ,  A61K31/4415 ,  A61K31/455 ,  A61K31/51 ,  A61K31/519 ,  A61K31/7076 ,  A61K38/27 ,  C12N9/1003 ,  C12Y201/01017 ,  G01N33/57484 ,  G01N2800/52

Abstract: The present invention provides methods for assessing contribution of one or more pathways to the biosynthesis of a metabolite, e.g., a lipid using the levels of the product made and the metabolite precursors for each pathway.

Abstract translation: 本发明提供用于评估一种或多种途径对代谢产物（例如使用制备的产物的水平和每个途径的代谢前体）的生物合成的贡献的方法。