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    Enhanced citric acid production in aspergillus with inactivated asparagine-linked glycosylation protein 3 (ALG3), and/or increased laeA expression
    1.
    发明授权
    Enhanced citric acid production in aspergillus with inactivated asparagine-linked glycosylation protein 3 (ALG3), and/or increased laeA expression 有权
    增强柠檬酸生产曲霉与灭活的天门冬酰胺连接的糖基化蛋白3（ALG3）和/或增加的laeA表达

    公开(公告)号：US09206450B2

    公开(公告)日：2015-12-08

    申请号：US14703499

    申请日：2015-05-04

    申请人： Battelle Memorial Institute

    发明人： Ziyu Dai ,  Scott E. Baker

    IPC分类号： C12P7/48 ,  C07K14/38

    CPC分类号： C12P7/48 ,  C07K14/38 ,  C12N1/14 ,  C12N9/1051 ,  C12Y204/01258

    摘要： Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.

    摘要翻译： 本文提供的是具有二里酰基-P-Man：Man（5）GlcNAc（2）-PP-二酰基甘露糖基转移酶（Alg3））基因失活的真菌，例如黑曲霉，增加的aflR表达A（Lae） ， 或两者。 在一些实例中，这样的突变体具有几种表型，包括相对于亲本菌株增加柠檬酸的产生。 还提供了使用所公开的真菌制备柠檬酸的方法，以及包括所公开的真菌的组合物和试剂盒。

    ENHANCED CITRIC ACID PRODUCTION IN ASPERGILLUS WITH INACTIVATED ASPARAGINE-LINKED GLYCOSYLATION PROTEIN 3 (ALG3), AND/OR INCREASED LAEA EXPRESSION
    3.
    发明申请
    ENHANCED CITRIC ACID PRODUCTION IN ASPERGILLUS WITH INACTIVATED ASPARAGINE-LINKED GLYCOSYLATION PROTEIN 3 (ALG3), AND/OR INCREASED LAEA EXPRESSION 有权
    增强的ASPERGILLUS中的柠檬酸生产与灭活的ASPARAGINE连接的糖蛋白3（ALG3）和/或增加的LAEA表达

    公开(公告)号：US20150232892A1

    公开(公告)日：2015-08-20

    申请号：US14703499

    申请日：2015-05-04

    申请人： BATTELLE MEMORIAL INSTITUTE

    发明人： Ziyu Dai ,  Scott E. Baker

    IPC分类号： C12P7/48 ,  C07K14/38

    CPC分类号： C12P7/48 ,  C07K14/38 ,  C12N1/14 ,  C12N9/1051 ,  C12Y204/01258

    摘要： Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.

    摘要翻译： 本文提供的是具有二里酰基-P-Man：Man（5）GlcNAc（2）-PP-二酰基甘露糖基转移酶（Alg3））基因失活的真菌，例如黑曲霉，增加的aflR表达A（Lae） ， 或两者。 在一些实例中，这样的突变体具有几种表型，包括相对于亲本菌株增加柠檬酸的产生。 还提供了使用所公开的真菌制备柠檬酸的方法，以及包括所公开的真菌的组合物和试剂盒。

    EXPRESSION OF MODIFIED GLYCOPROTEINS AND GLYCOPEPTIDES
    7.
    发明申请
    EXPRESSION OF MODIFIED GLYCOPROTEINS AND GLYCOPEPTIDES 审中-公开

    公开(公告)号：US20180251569A1

    公开(公告)日：2018-09-06

    申请号：US15967202

    申请日：2018-04-30

    申请人： Synthetic Genomics, Inc.

    发明人： NICKY C. CAIAZZA

    IPC分类号： C07K16/32 ,  C12N15/79 ,  C12N9/10

    CPC分类号： C07K16/32 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/41 ,  C12N9/1051 ,  C12N15/79 ,  C12Y204/01258

    摘要： The present invention provides recombinant cells that contain a genetic modification to at least one mannosyl transferase gene. As a result of the modification the cells produce a glycoprotein or glycopeptide that has an N-linked glycan profile that is simplified or more easily humanized. The glycoprotein or glycopeptide can have at least 25% fewer high mannose structures on than the glycoprotein or glycopeptide produced by a reference cell. In some embodiments the modification is a deletion or disruption of a mannosyl transferase gene, which can be in an alg3 gene. Therefore, the proteins produced are more useful for the production of therapeutic glycoproteins than those produced by species having foreign or plant-like patterns of glycosylation. The invention also provides compositions of the glycoproteins or glycopeptides and methods of making them.

    EXPRESSION OF MODIFIED GLYCOPROTEINS AND GLYCOPEPTIDES
    8.
    发明申请
    EXPRESSION OF MODIFIED GLYCOPROTEINS AND GLYCOPEPTIDES 审中-公开

    公开(公告)号：US20180119193A1

    公开(公告)日：2018-05-03

    申请号：US15799785

    申请日：2017-10-31

    申请人： SYNTHETIC GENOMICS, INC.

    发明人： Nicky C. Caiazza

    IPC分类号： C12P21/00 ,  C12N9/10 ,  C12N15/79 ,  C07K16/32

    CPC分类号： C12P21/005 ,  C07K16/32 ,  C07K2317/14 ,  C07K2317/41 ,  C12N9/1051 ,  C12N15/79 ,  C12Y204/01258

    摘要： The present invention provides recombinant cells that contain a modification causing altered expression or function of at least one mannosyl transferase enzyme. As a result of the modification the cells produce a glycoprotein or glycopeptide that has an N-linked glycan profile that is simplified or humanized. The glycoprotein or glycopeptide can have at least 25% fewer high mannose structures on than the glycoprotein or glycopeptide produced by a cell that does not have the modification. In some embodiments the modification is a deletion, knock out, or disruption of a gene encoding a mannosyl transferase, which can be in an Alg3 gene. Therefore, the proteins produced avoid many of the problems associated with the therapeutic use of glycoproteins from species having foreign or plant-like patterns of glycosylation. The invention also provides compositions of the glycoproteins or glycopeptides and methods of making them.

    ENHANCED ITACONIC ACID PRODUCTION IN ASPERGILLUS WITH INCREASED LAEA EXPRESSION
    9.
    发明申请
    ENHANCED ITACONIC ACID PRODUCTION IN ASPERGILLUS WITH INCREASED LAEA EXPRESSION 有权
    在增加的LAEA表达中增强了ASPERGILLUS中的ITACONIC酸生产

    公开(公告)号：US20160046967A1

    公开(公告)日：2016-02-18

    申请号：US14928511

    申请日：2015-10-30

    申请人： BATTELLE MEMORIAL INSTITUTE

    发明人： Ziyu Dai ,  Scott E. Baker

    IPC分类号： C12P7/44 ,  C07K14/38

    CPC分类号： C12P7/44 ,  C07K14/38 ,  C12N1/14 ,  C12N9/1051 ,  C12P7/48 ,  C12Y204/01258

    摘要： Fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (LaeA), or both, are described. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also described, as are compositions and kits including the disclosed fungi. Further described are Aspergillus terreus fungi overexpressing the LaeA gene and the use of such fungi for the production of itaconic acid.

    摘要翻译： 真菌，如黑曲霉，具有双酰基-P-Man：Man（5）GlcNAc（2）-PP-二酰基甘露糖基转移酶（Alg3）基因失活，增加了aflR表达A（LaeA）或两者的丧失表达 ，被描述。 在一些实例中，这样的突变体具有几种表型，包括相对于亲本菌株增加柠檬酸的产生。 还描述了使用所公开的真菌制备柠檬酸的方法，以及包括所公开的真菌的组合物和试剂盒。 进一步描述了过表达LaeA基因的土曲霉菌和使用这种真菌生产衣康酸。

    Enhanced citric acid production in Aspergillus with inactivated asparagine-linked glycosylation protein 3 (Alg3), and/or increased LaeA expression
    10.
    发明授权
    Enhanced citric acid production in Aspergillus with inactivated asparagine-linked glycosylation protein 3 (Alg3), and/or increased LaeA expression 有权
    增强柠檬酸生产曲霉与灭活天冬酰胺连接的糖基化蛋白3（Alg3）和/或增加的LaeA表达

    公开(公告)号：US09023637B2

    公开(公告)日：2015-05-05

    申请号：US13691396

    申请日：2012-11-30

    申请人： Battelle Memorial Institute

    发明人： Ziyu Dai ,  Scott E. Baker

    IPC分类号： C12N1/15 ,  C12P7/48 ,  C12N1/14 ,  C12N9/10 ,  C12N15/00 ,  C12P21/00 ,  C07H21/00

    CPC分类号： C12P7/48 ,  C12N1/14 ,  C12N9/1051 ,  C12Y204/01258

    摘要： Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.

    摘要翻译： 本文提供的是具有二里酰基-P-Man：Man（5）GlcNAc（2）-PP-二酰基甘露糖基转移酶（Alg3））基因失活的真菌，例如黑曲霉，增加的aflR表达A（Lae） ， 或两者。 在一些实例中，这样的突变体具有几种表型，包括相对于亲本菌株增加柠檬酸的产生。 还提供了使用所公开的真菌制备柠檬酸的方法，以及包括所公开的真菌的组合物和试剂盒。