公开(公告)号：US09234881B2

公开(公告)日：2016-01-12

申请号：US13700223

申请日：2011-05-27

申请人： Toshiharu Minoda ,  Isamu Ikeda ,  Satoru Nose ,  Noriaki Takeya ,  Ichiro Okamoto

发明人： Toshiharu Minoda ,  Isamu Ikeda ,  Satoru Nose ,  Noriaki Takeya ,  Ichiro Okamoto

IPC分类号： G01N30/90 ,  G01N30/94 ,  G01N30/92

CPC分类号： G01N30/90 ,  B01D15/20 ,  B01D15/265 ,  B01J20/045 ,  B01J20/26 ,  B01J20/3042 ,  G01N30/92 ,  G01N30/93 ,  G01N30/94 ,  G01N2030/945

摘要： A thin-layer chromatography plate and a thin-layer chromatography uses a thin-layer chromatography plate that has a first separating agent layer, which has the same optical response as the sample, and a second separating agent layer, which is disposed adjacent thereto and has an optical response that is different from the aforementioned optical response; carries out development of the sample in the first separating agent layer; thereafter carries out further development of the spots from the first separating agent layer toward the second separating agent layer; and detects the spots in the second separating agent layer through exposure to ultraviolet radiation or a color-development treatment with a coloring agent.

摘要翻译： 薄层色谱板和薄层色谱法使用具有与样品相同的光学响应的​​第一分离剂层的薄层色谱板和与其相邻设置的第二分离剂层， 具有与上述光学响应不同的光学响应; 在第一分离剂层中进行样品的开发; 然后进一步开发从第一分离剂层朝向第二分离剂层的斑点; 并通过暴露于紫外线照射或着色剂的显色处理来检测第二分离剂层中的斑点。

公开(公告)号：US20090081796A1

公开(公告)日：2009-03-26

申请号：US12159768

申请日：2007-07-31

申请人： Maria Irene De Oliveira Monteiro Jesus Rebelo ,  Belmiro Dos Santos Patricio

发明人： Maria Irene De Oliveira Monteiro Jesus Rebelo ,  Belmiro Dos Santos Patricio

IPC分类号： G01N30/94

CPC分类号： G01N30/94 ,  G01N2030/945

摘要： The present invention refers to a fast method for the determination of phospholipids in amniotic fluid samples by HPTLC (high performance thin layer chromatography). This innovative method presents high sensitivity, reproducibility and resolution. It has also the advantage of being of easy laboratorial interpretation, allowing the elimination of false results due to the contamination of the sample with blood or meconium.

摘要翻译： 本发明涉及通过HPTLC（高效薄层色谱法）测定羊水样品中磷脂的快速方法。 这种创新的方法具有高灵敏度，重复性和分辨率。 它还具有易于实验解释的优点，允许消除由于样品与血液或胎粪的污染导致的错误结果。

公开(公告)号：US20170299560A1

公开(公告)日：2017-10-19

申请号：US15486656

申请日：2017-04-13

申请人： CAMAG

发明人： Nicolas Richerdt ,  Eike Reich ,  Mark Howell Sturgess ,  Diego Micha Haldemann

IPC分类号： G01N30/94

CPC分类号： G01N30/94 ,  B05B5/00 ,  B05B7/0012 ,  B05B13/04 ,  B05B13/0405 ,  B05B16/60 ,  B05B17/0615 ,  B05B17/0638 ,  B05D1/02 ,  B05D1/04 ,  B05D1/30 ,  B05D1/60 ,  C23C16/4486 ,  C23C16/45563 ,  G01N2030/945

摘要： A derivatization apparatus and method for coating a sample carrier with a reagent solution. The derivatization apparatus consists of a closed system in which a small quantity of reagent solution is sprayed into a closed container via a spray nozzle. A sample carrier is located in the closed container and is coated as homogeneously as possible with the reagent solution.

公开(公告)号：US09726650B2

公开(公告)日：2017-08-08

申请号：US14375677

申请日：2013-02-01

申请人： DAICEL CORPORATION

发明人： Toshiharu Minoda ,  Isamu Ikeda

IPC分类号： B01D15/08 ,  G01N30/93 ,  G01N30/92 ,  B01J20/291 ,  B01J20/32 ,  B01J20/285 ,  G01N30/94 ,  G01N30/88

CPC分类号： G01N30/92 ,  B01D15/08 ,  B01J20/291 ,  B01J2220/46 ,  B01J2220/54 ,  G01N30/93 ,  G01N2030/8877 ,  G01N2030/945

摘要： A chromatographic medium having a separating agent layer, which is used to separate target substances, a filling agent layer, which is used to fix the target substances before the target substances are separated, and a permeation layer, which is used to enable permeation of the target substances separated by the separating agent layer, wherein the filling agent layer comes into contact with the separating agent layer via a plane that intersects the direction of development of the target substances in the chromatographic medium and is positioned on the upstream side in the direction of development, the separating agent layer exhibits separability of the target substances and optical responsiveness to ultraviolet rays, and the permeation layer exhibits an optical responsiveness that is different from those of the target substances and the separating agent layer.

公开(公告)号：US20130067996A1

公开(公告)日：2013-03-21

申请号：US13700223

申请日：2011-05-27

申请人： Toshiharu Minoda ,  Isamu Ikeda ,  Satoru Nose ,  Noriaki Takeya ,  Ichiro Okamoto

发明人： Toshiharu Minoda ,  Isamu Ikeda ,  Satoru Nose ,  Noriaki Takeya ,  Ichiro Okamoto

IPC分类号： G01N30/90

CPC分类号： G01N30/90 ,  B01D15/20 ,  B01D15/265 ,  B01J20/045 ,  B01J20/26 ,  B01J20/3042 ,  G01N30/92 ,  G01N30/93 ,  G01N30/94 ,  G01N2030/945

摘要： The present invention provides a thin-layer chromatography plate and a thin-layer chromatography that can detect the separation of a component in a sample by a separating agent layer that does not permit the detection of the separation of the component in the sample by an optical response. For a sample that has an optical response to ultraviolet light or a coloring agent, the present invention uses a thin-layer chromatography plate that has a first separating agent layer, which has the same optical response as the sample, and a second separating agent layer, which is disposed adjacent thereto and has an optical response that is different from the aforementioned optical response; carries out development of the sample in the first separating agent layer; thereafter carries out further development of the spots from the first separating agent layer toward the second separating agent layer; and detects the spots in the second separating agent layer through exposure to ultraviolet radiation or a color-development treatment with a coloring agent. In addition, the development axis in the first separating agent layer is maintained approximately perpendicular, and spot broadening at the boundary surface is prevented by adjusting the binder blending ratio.

摘要翻译： 本发明提供薄层色谱板和薄层色谱法，其可以通过分离剂层检测样品中组分的分离，所述分离剂层不允许通过光学检测样品中组分的分离 响应。 对于具有对紫外线或着色剂的光学响应的​​样品，本发明使用具有与样品相同的光学响应的​​第一分离剂层的薄层色谱板和第二分离剂层 ，其与其相邻布置并具有与上述光学响应不同的光学响应; 在第一分离剂层中进行样品的开发; 然后进一步开发从第一分离剂层朝向第二分离剂层的斑点; 并通过暴露于紫外线照射或着色剂的显色处理来检测第二分离剂层中的斑点。 此外，第一分离剂层中的显影轴保持大致垂直，并且通过调节粘合剂共混比来防止边界表面的斑点变宽。

公开(公告)号：US20070077660A1

公开(公告)日：2007-04-05

申请号：US11238974

申请日：2005-09-30

申请人： Ronald Glas

发明人： Ronald Glas

IPC分类号： G01N33/00

CPC分类号： G01N30/90 ,  G01N2030/945 ,  Y10T436/142222

摘要： The invention relates to a method and detection kit for the detection of a cannabinoid in a cannabis sample. The method is based on TLC and the method and kit enables everyone to avoid laborious, time consuming and expensive analytical research and determine the cannabinoid content. Good results are obtained with a developing solvent with at least 25 vol. % chloroform and at least 25 vol. % 1,2-dichloro ethane.

摘要翻译： 本发明涉及用于在大麻样品中检测大麻素的方法和检测试剂盒。 该方法基于TLC，方法和试剂盒使每个人都能避免费力，耗时和昂贵的分析研究，并确定大麻素含量。 使用至少25体积的显影溶剂获得良好的结果。 ％氯仿和至少25体积％ ％1,2-二氯乙烷。

公开(公告)号：US20140374333A1

公开(公告)日：2014-12-25

申请号：US14375677

申请日：2013-02-01

申请人： DAICEL CORPORATION

发明人： Toshiharu Minoda ,  Isamu Ikeda

IPC分类号： G01N30/92 ,  B01J20/291 ,  B01D15/08

CPC分类号： G01N30/92 ,  B01D15/08 ,  B01J20/291 ,  B01J2220/46 ,  B01J2220/54 ,  G01N30/93 ,  G01N2030/8877 ,  G01N2030/945

摘要： Provided is a chromatographic medium capable of separating and detecting target substances without the use of other components. A chromatographic medium having a separating agent layer, which is used to separate target substances, a filling agent layer, which is used to fix the target substances before the target substances are separated, and a permeation layer, which is used to enable permeation of the target substances separated by the separating agent layer, wherein the filling agent layer comes into contact with the separating agent layer via a plane that intersects the direction of development of the target substances in the chromatographic medium and is positioned on the upstream side in the direction of development, the separating agent layer exhibits separability of the target substances and exhibits optical responsiveness to ultraviolet rays, and the permeation layer exhibits optical responsiveness that are different from those of the target substances and the separating agent layer.

摘要翻译： 提供了能够在不使用其他成分的情况下分离检测目标物质的色谱介质。 具有用于分离目标物质的分离剂层的色谱介质，用于在目标物质分离之前固定目标物质的填充剂层和用于使得能够渗透的目的物质的渗透层 由分离剂层分离的目标物质，其中所述填充剂层经由与所述色谱介质中的所述目标物质的显影方向相交的平面与所述分离剂层接触，并且位于所述分离剂层的方向上的上游侧 开发时，分离剂层表现出目标物质的分离性并且对紫外线表现出光学响应性，并且渗透层表现出与目标物质和分离剂层不同的光学响应性。

公开(公告)号：US20060160127A1

公开(公告)日：2006-07-20

申请号：US11384960

申请日：2006-03-20

申请人： Detlev Hadbawnik ,  Christian Wenz

发明人： Detlev Hadbawnik ,  Christian Wenz

IPC分类号： C12Q1/68

CPC分类号： G01N27/44739 ,  G01N30/94 ,  G01N35/1074 ,  G01N2001/2873 ,  G01N2030/945

摘要： Disclosed is the extraction of molecules from at least one separation medium wherein a frame with different compartments is brought in contact with the separation medium, thereby dividing the separation medium into different compartments. At least one solvent is applied into the different compartments, extracting the molecules.

摘要翻译： 公开了从至少一种分离介质中提取分子，其中具有不同隔室的框架与分离介质接触，从而将分离介质分成不同的隔室。 将至少一种溶剂施加到不同的隔室中，提取分子。

公开(公告)号：US20050269267A1

公开(公告)日：2005-12-08

申请号：US11084501

申请日：2005-03-18

申请人： Wayne Patton ,  Mack Schermer

发明人： Wayne Patton ,  Mack Schermer

IPC分类号： B01D15/08 ,  C07K1/26 ,  G01N27/447

CPC分类号： C07K1/26 ,  C07K1/285 ,  G01N27/44747 ,  G01N30/58 ,  G01N30/92 ,  G01N2030/8813 ,  G01N2030/945

摘要： The present invention describes a system and method for separation of proteins, peptides and glycans by one-dimensional or two-dimensional electroosmosis-driven planar chromatography. Separation is performed using amphiphilic polymeric membranes, amphiphilic thin-layer chromatography plates or other planar amphiphilic surfaces as the stationary phase with a combination of organic and/or aqueous buffers as the mobile phase. Systematic selection of stationary phase supports, mobile phase buffers and operating conditions allow for the adaptation of the invention to a broad range of applications in proteomics, mass spectrometry, drug discovery and the pharmaceutical sciences.

摘要翻译： 本发明描述了通过一维或二维电渗驱动的平面色谱分离蛋白质，肽和聚糖的系统和方法。 使用两亲聚合物膜，两亲性薄层色谱板或其它平面两亲性表面作为固定相进行分离，其中有机和/或含水缓冲液的组合作为流动相。 固定相载体，流动相缓冲液和操作条件的系统选择允许本发明适用于蛋白质组学，质谱，药物发现和药学科学领域的广泛应用。

公开(公告)号：US5077221A

公开(公告)日：1991-12-31

申请号：US557889

申请日：1990-07-25

申请人： Theodore E. Miller, Jr. ,  Melanie C. Poon

发明人： Theodore E. Miller, Jr. ,  Melanie C. Poon

IPC分类号： G01N30/02 ,  G01N30/94 ,  G01N31/22 ,  G01N33/18

CPC分类号： G01N30/94 ,  G01N2030/945 ,  G01N30/02 ,  G01N31/22 ,  G01N33/18

摘要： A chemical analysis method for indirect detection thin layer chromatography of cations that provides a rapid indication of the amount and type of cation present with minimal equipment requirements. The method includes four steps. The first step is to equilibrate a thin layer cation exchange chromatography plate with a color-forming cation, such as with copper II, to form a prepared plate. The second step is to deposit a sample on the prepared plate to form a spotted plate, the sample containing a cation of interest, such as sodium I. The third step is to develop the spotted plate with a liquid solution containing the color-forming cation, such as a solution of copper II ions, to chromatograph the cation of the sample to form a developed plate. The last step is to expose the developed plate to a chemical agent, such as ammonia, to visualize the color-forming cation by a chemical reaction between the chemical agent and the color-forming cation to form a colored species, such as the blue colored copper II/ammonia complex. When this is done, the chromatographed sample cation is apparent as region of less intense color in a field of color on the plate generated by the colored species.

摘要翻译： 用于阳离子间接检测薄层色谱的化学分析方法，可以最小化设备要求，提供阳离子的数量和类型的快速指示。 该方法包括四个步骤。 第一步是平衡具有着色形成阳离子的薄层阳离子交换层析板，例如用铜II，以形成制备的板。 第二步是将样品沉积在制备的板上以形成点样板，样品含有目标阳离子，例如I.I。第三步是用含有成色阳离子的液体溶液显影点样板 ，例如铜II离子的溶液，对样品的阳离子进行色谱以形成显影板。 最后一步是将显影的板暴露于化学试剂如氨，以通过化学试剂和成色阳离子之间的化学反应来显现成色阳离子，以形成有色物质，例如蓝色 铜II /氨络合物。 当这样做时，色谱样品阳离子在由着色物质产生的板上的颜色领域中不太强烈的颜色的区域是明显的。