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    Secreted Chlamydia polypeptides and method for identifying such polypeptides by their secretion by a type III secretion pathway of a gram-negative bacteria
    2.
    发明申请
    Secreted Chlamydia polypeptides and method for identifying such polypeptides by their secretion by a type III secretion pathway of a gram-negative bacteria 审中-公开
    分泌衣原体多肽和通过其由革兰氏阴性细菌的III型分泌途径分泌来鉴定此类多肽的方法

    公开(公告)号：US20030045702A1

    公开(公告)日：2003-03-06

    申请号：US10014670

    申请日：2001-12-14

    申请人： INSTITUT PASTEUR

    发明人： Agathe Subtil ,  Claude Parsot ,  Alice Dautry-Varsat

    IPC分类号： A61K039/02 ,  C07H021/04 ,  C12N001/21 ,  C12P021/02 ,  C07K014/295

    CPC分类号： C07K14/295 ,  A61K39/00

    摘要： Chlamydia spp. are strictly intracellular pathogens that grow inside a vacuole, called an inclusion. They possess genes encoding proteins homologous to components of type III secretion machineries which, in other bacterial pathogens, are involved in delivery of bacterial proteins within or through the membrane of eukaryotic host cells. Inc proteins are chlamydial proteins that are associated with the membrane of the inclusion and are characterized by the presence of a large hydrophobic domain in their amino acid sequence. To investigate whether some Chlamydia proteins, especially Inc proteins and other proteins exhibiting a similar hydropathic profile, might be secreted, the inventors used an heterologous secretion system, namely a type III system. Chimeras were constructed by fusing the N-terminal part of these proteins with a reporter, the Cya protein of Bordetella pertussis, and expressed in various strains of Shigella flexneri. The inventors demonstrate that these hybrid proteins are secreted by the type III secretion system of S. flexneri. Moreover, the inventors show that three other proteins from C. pneumoniae, all of which have in common the presence of a large hydrophobic domain, are also secreted by S. flexneri type III secretion machinery.

    摘要翻译： 衣原体属 是严格细胞内的病原体，在液泡内生长，称为包涵体。 它们具有编码与III型分泌机制的组分同源的蛋白质的基因，其在其它细菌病原体中涉及在真核宿主细胞的膜内或通过真核宿主细胞的膜中递送细菌蛋白质。 Inc蛋白是与包涵体膜相关的衣原体蛋白，其特征在于在其氨基酸序列中存在大的疏水结构域。 为了研究是否可能分泌某些衣原体蛋白，特别是Inc蛋白质和其他表现出类似亲水性谱的蛋白质，本发明人使用异源分泌系统，即III型系统。 嵌合体通过将这些蛋白质的N末端部分与报告基因（百日咳博德特氏菌的Cya蛋白质）融合并在各种不同的志贺氏菌属菌株中表达来构建。 本发明人证明这些杂交蛋白质是由柔毛氏菌的III型分泌系统分泌的。 此外，本发明人表明，来自肺炎克雷伯杆菌的三种其它蛋白质都具有大的疏水结构域的共同作用，也由S.perflexi III型分泌机制分泌。

    Chlamydia pmp proteins, gene sequences and uses thereof
    5.
    发明申请
    Chlamydia pmp proteins, gene sequences and uses thereof 失效
    衣原体pmp蛋白，基因序列及其用途

    公开(公告)号：US20040037846A1

    公开(公告)日：2004-02-26

    申请号：US10398248

    申请日：2003-08-01

    发明人： W. James Jackson

    IPC分类号： C07K014/295 ,  C12Q001/68 ,  C07H021/04 ,  A61K039/02 ,  C12P021/02 ,  C12N001/21

    CPC分类号： A61K39/118 ,  A01K2227/105 ,  A01K2267/0337 ,  A61K39/00 ,  A61K49/0008 ,  A61K2039/505 ,  A61K2039/543 ,  A61K2039/55544 ,  C07H21/04 ,  C07K14/295 ,  C07K16/125 ,  C12Q1/6893 ,  G01N33/56927 ,  G01N2333/295

    摘要： The invention discloses the Chlamydia PMPE and PMPI polypeptide, polypeptides derived therefrp, (PMP-derived polypeptides), nucleotide sequences encoding said polypeptides, antibodies that specifically bind the PMP polypeptides and PMP-derived polypeptides and T-cells specific for PMP polypeptides and PMP-derived polypeptides. Also disclosed are prophylactic and therapeutic compositions, including immunogenic compositions, e.g., vaccines, comprising PMP polypeptides or PMP-derived polypeptides or antibodies thereto. The invention additionally discloses methods of inducing in animals an immune response to Chlamydia cells, Chlamydia elementary bodies, and/or cells expressing Chlamydial proteins, e.g., cell infected with Chlamydia.

    摘要翻译： 本发明公开了衣原体PMPE和PMPI多肽，来自其的多肽，（PMP衍生的多肽），编码所述多肽的核苷酸序列，特异性结合PMP多肽和PMP衍生多肽的抗体以及对PMP多肽和PMP- 衍生的多肽。 还公开了预防和治疗组合物，包括免疫原性组合物，例如包含PMP多肽或PMP衍生的多肽或其抗体的疫苗。 本发明还公开了在动物中诱导对衣原体细胞，衣原体基因体和/或表达衣原体蛋白（例如感染衣原体的细胞）的细胞的免疫应答的方法。

    CHLAMYDIA ANTIGENS AND CORRESPONDING DNA FRAGMENTS AND USES THEREOF
    10.
    发明申请
    CHLAMYDIA ANTIGENS AND CORRESPONDING DNA FRAGMENTS AND USES THEREOF 审中-公开
    CHLAMYDIA抗原和相应的DNA片段及其用途

    公开(公告)号：US20030147924A1

    公开(公告)日：2003-08-07

    申请号：US09361040

    申请日：1999-07-26

    申请人： AVENTIS PASTEUR LIMITED/AVENTIS PASTEUR LIMITEE

    发明人： ANDREW D. MURDIN ,  RAYMOND P. OOMEN

    IPC分类号： A61K039/118 ,  C07H021/04 ,  C12P021/02 ,  C12N001/21 ,  C07K014/295 ,  C12N015/74

    CPC分类号： C07K14/295 ,  A61K39/00 ,  A61K2039/51 ,  C07K2319/00 ,  C07K2319/02

    摘要： In summary of this disclosure, the present invention provides a method of nucleic acid, including DNA, immunization of a host, including humans, against disease caused by infection by a strain of Chlamydia, specifically C. pneumoniae, employing a vector, containing a nucleotide sequence encoding a CPN100149 polypeptide of a strain of Chlamydia pneumoniae and a promoter to effect expression of the CPN100149 polypeptide in the host. Modifications are possible within the scope of this invention.

    摘要翻译： 综上所述，本发明提供了一种核酸的方法，包括DNA，包括人在内的宿主的免疫，免疫衣原体菌株，特别是肺炎衣原体感染引起的疾病，使用含有核苷酸的载体 编码肺炎衣原体菌株的CPN100149多肽的序列和在宿主中影响CPN100149多肽表达的启动子。 在本发明的范围内可以进行修改。