公开(公告)号：US20020132285A1

公开(公告)日：2002-09-19

申请号：US10058466

申请日：2002-01-28

申请人： Idexx Laboratories, Inc.

发明人： Chung-Ming Chen ,  Stephen C. Edberg

IPC分类号： G01N033/53 ,  G01N033/567 ,  G01N033/571 ,  C12Q001/52 ,  C12Q001/02 ,  C12Q001/04 ,  C12Q001/14 ,  C12Q001/10 ,  C12Q001/06 ,  C12P033/02 ,  C12N001/00 ,  C12N001/20

CPC分类号： C12N1/20 ,  C12Q1/045 ,  C12Q1/10 ,  C12Q1/18 ,  C12Q1/34 ,  C12Q1/54 ,  Y10S435/885

摘要： A microbe-specific medium for detection of vancomycin-resistant Enterococci in a test sample within 24 hours and preferably within 18 hours. The testing medium provides a selective growth medium for vancomycin-resistant Enterococci and includes specific nutrient indicators which only the target microbe can significantly metabolize and use for growth. The nutrient indicator contain a nutrient moiety and a detectable moiety linked together by a covalent bond. The nutrient indicators produce detectable signals only if the nutrient indicators are hydrolyzed by the Enterococci specific enzymes including null-glucosidase and pyrrolidonyl arylamidase.

公开(公告)号：US20010055784A1

公开(公告)日：2001-12-27

申请号：US09858986

申请日：2001-05-16

申请人： ARKRAY, Inc.

发明人： Yuichiro Noda ,  Yoshiyuki Tanaka ,  Konomu Hirao

IPC分类号： G01N021/78 ,  C12Q001/37 ,  C12Q001/40 ,  C12Q001/42 ,  C12Q001/50 ,  C12Q001/52 ,  C12Q001/60 ,  C12Q001/62

CPC分类号： G01N33/96 ,  Y10T436/10 ,  Y10T436/193333 ,  Y10T436/25 ,  Y10T436/255

摘要： Amounts of components in a specimen can be analyzed with excellent quantitativity. The analysis includes: measuring an amount of a component to be analyzed in a specimen; measuring an amount of a standard component present originally and homeostatically in the specimen other than the component to be analyzed; determining the amount of the specimen from the amount of the standard component thus measured and a known concentration of the standard component in the specimen; and determining a concentration of the component to be analyzed in the specimen from the amount of the specimen thus determined and the amount of the component to be analyzed thus measured. The quantitative analysis of the present invention allows a component to be analyzed to be measured with high quantitativity as shown in FIG. 1.

摘要翻译： 可以以极好的定量分析样品中组分的量。 分析包括：测量样品中要分析的组分的量; 测量除了要分析的组分之外的样品中最初和稳定存在的标准组分的量; 从所测量的标准组分的量和样品中标准组分的已知浓度确定样品的量; 以及根据如此测定的试样的量和待测量的成分的量来测定试样中要分析的成分的浓度。 本发明的定量分析允许如图1所示以高定量度量测分析成分。 1。

公开(公告)号：US20020098533A1

公开(公告)日：2002-07-25

申请号：US10027204

申请日：2001-12-21

发明人： George L. King

IPC分类号： C12Q001/52

CPC分类号： G01N33/6893 ,  C12Q1/485 ,  G01N2800/042 ,  G01N2800/32

摘要： The invention features a method for evaluating PKC activity in vascular tissues. The invention also features methods for diagnosing cardiovascular and diabetes related disorders, and for identifying and evaluating treatments for cardiovascular or diabetes related disorders. Methods for identifying and evaluating treatments for aging are also included. The methods include measuring PKC activity in monocytes as a surrogate for PKC activity in other tissues.

摘要翻译： 本发明的特征在于评估血管组织中PKC活性的方法。 本发明还具有用于诊断心血管和糖尿病相关疾病以及用于鉴定和评估心血管或糖尿病相关疾病的治疗的方法。 还包括鉴定和评估老化治疗方法。 这些方法包括测量单核细胞中的PKC活性，作为其他组织中PKC活性的替代物。

公开(公告)号：US20020034767A1

公开(公告)日：2002-03-21

申请号：US09851686

申请日：2001-05-08

发明人： Jeffrey L. Benovic ,  Jorge Gomez ,  Priya Kunapuli

IPC分类号： G01N033/53 ,  C12Q001/52

CPC分类号： C12N9/1205 ,  C12N2799/026

摘要： G protein-coupled receptor kinases (GRK) play an important role in phosphorylating and regulating the activity of G protein-coupled receptors. Complementary DNAs (cDNAs)that encode two novel members of the G protein-coupled receptor kinase (GRK) family are provided in the present invention. These cDNAs encode GRK5 (590 amino acids) and GRK6 (576 amino acids) which represent two new members of the GRK family that have distinct tissue distribution and substrate specificity. The availability of the cDNAs enables the generation of reagents to modulate the activity of endogenous kinases. These include dominant negative mutations and antisense oligonucleotides or stably transfected antisense constructs to block expression of the kinase to generate a cell with a reduced ability to desensitize to various agents. Expression of GRK5 and GRK6 also permits identification of specific inhibitors and activators of these two kinases. Such inhibitors and activators may be used therapeutically to either directly modulate the activity of a given receptor or by augmenting the ability of a given therapeutic agent to stimulate a given receptor.

摘要翻译： G蛋白偶联受体激酶（GRK）在磷酸化和调节G蛋白偶联受体的活性中起重要作用。 在本发明中提供了编码G蛋白偶联受体激酶（GRK）家族的两个新成员的互补DNA（cDNA）。 这些cDNA编码GRK5（590个氨基酸）和GRK6（576个氨基酸），其代表具有不同组织分布和底物特异性的GRK家族的两个新成员。 cDNA的可用性使得能够产生用于调节内源性激酶活性的试剂。 这些包括显性阴性突变和反义寡核苷酸或稳定转染的反义构建体以阻断激酶的表达以产生具有降低对各种试剂的敏感能力的细胞。 GRK5和GRK6的表达还可以鉴定这两种激酶的特异性抑制剂和活化剂。 这样的抑制剂和活化剂可以用于治疗性地直接调节给定受体的活性或增加给定治疗剂刺激给定受体的能力。

公开(公告)号：US20020037545A1

公开(公告)日：2002-03-28

申请号：US10003597

申请日：2001-10-30

发明人： Qinghong Han ,  Li Tang ,  Mingxu Xu ,  Yuying Tan ,  Shigeo Yagi

IPC分类号： C12Q001/52

CPC分类号： C12Q1/48 ,  G01N2333/91011

摘要： A method to assess the level of folate in a biological sample comprises. providing said sample with glycine N-methyltransferase (GMT) and with an excess of S-adenosyl methionine (SAM) and of glycine; providing a control which contains no folate with said GMT and excess SAM and glycine in comparable amounts to those provided to the sample; and comparing the concentration of at least one product formed in the sample with the concentrations of said product formed in the control, whereby the difference in levels of said product in the sample as compared to the control is directly proportional to the level of folate in the sample. Also disclosed is a method to detect and measure the concentration of analytes which can be subjected to protocols that generate hydrogen peroxide. This method comprises measuring the level of hydrogen peroxide by adding peroxidase and a dialkyl phenylene diamine.

摘要翻译： 评估生物样品中叶酸水平的方法包括。 向所述样品提供甘氨酸N-甲基转移酶（GMT）和过量的S-腺苷甲硫氨酸（SAM）和甘氨酸; 提供一个对照，其中不含叶酸与所述GMT和多余的SAM和甘氨酸与提供给样品的量相当; 并将样品中形成的至少一种产物的浓度与在对照中形成的所述产物的浓度进行比较，由此与对照相比，样品中所述产物的水平差与叶酸中的叶酸水平成正比 样品。 还公开了一种检测和测量可以经历产生过氧化氢的方案的分析物的浓度的方法。 该方法包括通过加入过氧化物酶和二烷基苯二胺来测量过氧化氢的含量。

公开(公告)号：US20010044127A1

公开(公告)日：2001-11-22

申请号：US09850031

申请日：2001-05-08

申请人： AJINOMOTO CO., INC.

发明人： Nobuhisa Shimba ,  Eiichiro Suzuki ,  Keiichi Yokoyama

IPC分类号： C12Q001/52 ,  G01N033/53 ,  G01N033/542

CPC分类号： C07K5/06104 ,  G01N33/534 ,  G01N2333/9108

摘要： The present invention provides a method for isotopically labeling a functional group possessed by an amino acid residue of a protein. The present invention also provides a protein whose functional group in an amino acid residue is isotopically labeled. A functional group in an amino acid residue of a protein is substituted with an isotope-labeling group derived from an isotope-labeling compound by making use of the action of an enzyme. In particular, the carboxyamide nitrogen atom in a glutamine residue of a protein is replaced with an isotopically labeled atom by acting a transglutaminase on the glutamine residue.

摘要翻译： 本发明提供一种同位素标记由蛋白质的氨基酸残基所具有的官能团的方法。 本发明还提供了其氨基酸残基中的官能团被同位素标记的蛋白质。 通过利用酶的作用，由同位素标记化合物衍生的同位素标记基团取代蛋白质的氨基酸残基中的官能团。 特别地，蛋白质的谷氨酰胺残基中的羧酰胺氮原子被同位素标记的原子取代，通过在谷氨酰胺残基上起转谷氨酰胺酶的作用。

公开(公告)号：US20010039005A1

公开(公告)日：2001-11-08

申请号：US09767790

申请日：2001-01-23

发明人： Murali Ramanathan ,  Marilyn E. Morris

IPC分类号： C12Q001/00 ,  G01N033/53 ,  C12Q001/48 ,  C12Q001/52 ,  C12P019/38 ,  C12P019/40 ,  C12P007/10 ,  C12P005/00 ,  C12N001/00

CPC分类号： G01N33/542 ,  G01N2333/765

摘要： The present invention relates to a method of screening for drug binding to serum proteins by: preparing at least two solutions each including a concentration of a serum protein and a concentration of a candidate drug, wherein the concentration of the candidate drug is different for each of the at least two solutions; exposing each of the at least two solutions to a light source; measuring fluorescent emission by the serum protein or a serum protein-candidate drug complex for each of the at least two solutions upon said exposing; and determining whether a change in fluorescence emission is measured for an increased concentration of the candidate drug, wherein the change in fluorescence emission indicates binding of the candidate drug to the serum protein. A kit useful for performing a fluorimetric screening of drug binding to serum proteins is also disclosed.

摘要翻译： 本发明涉及通过以下方法筛选药物结合的方法：制备每种包含血清蛋白浓度和候选药物浓度的至少两种溶液，其中候选药物的浓度对于每种 至少两个解决方案; 将所述至少两种溶液中的每一种暴露于光源; 在所述暴露时测量所述至少两种溶液中的每一种的血清蛋白或血清蛋白候选药物复合物的荧光发射; 并测定荧光发射的变化是否增加候选药物的浓度，其中荧光发射的变化表明候选药物与血清蛋白的结合。 还公开了用于进行药物与血清蛋白结合的荧光筛选的试剂盒。

公开(公告)号：US20030186837A1

公开(公告)日：2003-10-02

申请号：US10012991

申请日：2001-12-10

发明人： Sze-Chung Lo ,  Maria Victoria Montenegro-Chamorro ,  Sheryl Frank ,  Blaise Darveaux ,  Sanjoy Mahanty ,  Ryan Heiniger ,  Amy Skalchunes ,  Huaqin Pan ,  Rex Tarper ,  Jeffrey Shuster ,  Matthew M. Tanzer ,  Lisbeth Hamer ,  Kiichi Adachi ,  Todd M. DeZwaan

IPC分类号： A61K031/00 ,  C12Q001/52 ,  C07H021/04 ,  C12Q001/18 ,  C12N009/10 ,  C12N001/16 ,  C12P021/02 ,  C12N015/74

CPC分类号： C12N9/93 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12Q1/18 ,  Y10S514/924

摘要： The present inventors have discovered that Asparagine Synthase is essential for fungal pathogenicity. Specifically, the inhibition of Asparagine Synthase gene expression in fungi results in no signs of successful infection or lesions. Thus, Asparagine Synthase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit Asparagine Synthase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

摘要翻译： 本发明人已经发现天门冬酰胺合酶对于真菌致病性是必需的。 具体来说，真菌中天冬酰胺合酶基因表达的抑制没有导致成功感染或病变的迹象。 因此，天门冬酰胺合酶可用作鉴定抗生素，优选抗真菌剂的靶标。 因此，本发明提供了鉴定抑制天门冬酰胺合酶表达或活性的化合物的方法。 本发明的方法可用于鉴定抗生素，优选抗真菌剂。

公开(公告)号：US20020127627A1

公开(公告)日：2002-09-12

申请号：US10027938

申请日：2001-12-19

发明人： Randy Ranjee Ramharack ,  Mark Allan Spahr

IPC分类号： C12Q001/48 ,  C12Q001/52

CPC分类号： C12Q1/48 ,  G01N2500/00 ,  G01N2500/02

摘要： The present invention provides a method for measuring diacylglycerol acetyltransferase (DGAT) activity which utilizes a novel solvent system to reduce and/or eliminate the activities of related compounds. The present invention also discloses a method for determining whether a compound is useful for modulating DGAT biological activity. The method is capable of being utilized for mass screening of compounds as modulators of the biological activity of DGAT.

摘要翻译： 本发明提供了一种测量二酰基甘油乙酰转移酶（DGAT）活性的方法，其利用新的溶剂体系来减少和/或消除相关化合物的活性。 本发明还公开了一种用于确定化合物是否可用于调节DGAT生物活性的方法。 该方法能够用于大规模筛选化合物作为DGAT生物活性的调节剂。

公开(公告)号：US20020009763A1

公开(公告)日：2002-01-24

申请号：US09805193

申请日：2001-03-14

申请人： Nitto Boseki Co., Ltd.

发明人： Yoshiro Sato ,  Chie Satokawa ,  Ryo Kojima ,  Katsuhiro Katayama

IPC分类号： C12Q001/52

CPC分类号： C12Q1/32 ,  C12Q1/52

摘要： A two reagent-type kit for assaying GPT by acting GPT on L-alanine and null-ketoglutarate in the presence of pyridoxal phosphate, converting the resulting pyruvate into lactate with L-lactate dehydrogenase (LDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH) and measuring GPT based on a decrement of NADH, the GPT assay kit comprising a first reagent and a second reagent, one of which contains pyridoxal phosphate, L-alanine, null-ketoglutarate, LDH and NADH and the other reagent contains no pyridoxal phosphate but contains L-alanine. The GPT assay kit is stable over a long period of time.

摘要翻译： 在吡哆醛磷酸盐存在下，通过对L-丙氨酸和α-酮戊二酸作用GPT来测定GPT的两种试剂型试剂盒，在还原的烟酰胺腺嘌呤二核苷酸存在下，将所得丙酮酸转化成乳酸L-乳酸脱氢酶（LDH） NADH），并且基于NADH的减少测量GPT，GPT测定试剂盒包含第一试剂和第二试剂，其中一种试剂含有磷酸吡哆醛，L-丙氨酸，α-酮戊二酸，LDH和NADH，另一种试剂不含吡哆醛 磷酸盐但含有L-丙氨酸。 GPT测定试剂盒在长时间内是稳定的。