公开(公告)号：US06448011B1

公开(公告)日：2002-09-10

申请号：US09688415

申请日：2000-10-16

申请人： Jonathan A. Bard ,  Richard L. Weinshank ,  Carlos C. Forray

发明人： Jonathan A. Bard ,  Richard L. Weinshank ,  Carlos C. Forray

IPC分类号： C12Q1568

CPC分类号： C12Q1/6876 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/10 ,  A01K2227/105 ,  A01K2267/02 ,  A01K2267/03 ,  A01K2267/0393 ,  A61K38/00 ,  C07K14/70571 ,  C12N15/85 ,  C12N15/8509 ,  C12Q1/6897 ,  C12Q2600/158 ,  G01N33/9433 ,  G01N2500/10

摘要： This invention provides an isolated nucleic acid, vectors, transformed mammalian cells and non-human transgenic animals that encode and express normal or mutant alpha 1a, alpha 1b and alpha 1c adrenergic receptor genes. This invention also provides a protein, and an antibody directed to the protein and pharmaceutical compounds related to alpha 1a, alpha 1b and alpha 1c adrenergic receptors. This invention provides nucleic acid probes, and antisense oligonucleotides complementary to alpha 1a, alpha 1b and alpha 1c adrenergic receptor genes. This invention further provides methods for determining ligand binding, detecting expression, drug screening, and treatments for alleviating abnormalities associated with human alpha 1a, alpha 1b and alpha 1c adrenergic receptors.

摘要翻译： 本发明提供了分离的核酸，载体，转化的哺乳动物细胞和编码并表达正常或突变的α1a，α1b和α1c肾上腺素能受体基因的非人转基因动物。 本发明还提供了一种蛋白质和针对与α1a，α1b和α1c肾上腺素能受体相关的蛋白质和药物化合物的抗体。 本发明提供核酸探针和与α1a，α1b和α1c肾上腺素能受体基因互补的反义寡核苷酸。 本发明还提供了确定配体结合，检测表达，药物筛选和治疗以减轻与人α1a，α1b和α1c肾上腺素能受体相关的异常的方法。

公开(公告)号：US06534263B1

公开(公告)日：2003-03-18

申请号：US09419406

申请日：1999-10-15

申请人： Scott E. Plevy ,  Jerome I. Rotter ,  Stephan R. Targan ,  Hiroo Toyoda ,  Huiying Yang

发明人： Scott E. Plevy ,  Jerome I. Rotter ,  Stephan R. Targan ,  Hiroo Toyoda ,  Huiying Yang

IPC分类号： C12Q1568

CPC分类号： C12Q1/6858 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/172

摘要： A novel association between certain tumor necrosis factor microsatellite alleles and Crohn's disease has been discovered. In accordance with the present invention, there is provided methods for screening for Crohn's disease comprising detecting the presence or absence of nucleic acid of a subject encoding TNF microsatellite alleles associated with Crohn's disease, wherein the presence of nucleic acid encoding three or more of the alleles is indicative of Crohn's disease. Kits useful for screening for Crohn's disease are also provided which comprise nucleic acid encoding TNF microsatellite alleles associated with Crohn's disease.

摘要翻译： 已经发现了某些肿瘤坏死因子微卫星等位基因与克罗恩病之间的关联。 根据本发明，提供了用于筛选克罗恩病的方法，包括检测编码与克罗恩病相关的TNF微卫星等位基因的受试者的核酸的存在或不存在，其中编码三个或更多个等位基因的核酸的存在 表明克罗恩病。 还提供了用于筛选克罗恩病的试剂盒，其包含编码与克罗恩病相关的TNF微卫星等位基因的核酸。