公开(公告)号：US20220272956A1

公开(公告)日：2022-09-01

申请号：US17622770

申请日：2020-06-23

申请人： ZHEJIANG UNIVERSITY

发明人： Mingying YANG ,  Jie WANG ,  Yuyin CHEN ,  Ying ZHANG

IPC分类号： A01K67/04 ,  A23K20/147 ,  C09K11/02 ,  C09K11/77 ,  B82Y99/00

摘要： Disclosed is a method for constructing fluorescent Bombyx Mori silkworm silk irradiated by near-infrared light and its products, comprising: (1) preparing upconversion nanoparticles, and performing surface modification with concanavalin to obtain modified upconversion nanoparticles; (2) uniformly dispersing the modified upconversion nanoparticles in water to formulate an aqueous solution of the upconversion nanoparticles, (3) picking mature mulberry leaves, immersing the mulberry leaves in the aqueous solution system of the nanoparticles, leaching water, and naturally drying the mulberry leaves; (4) after silkworms have grown for a set time, feeding the treated mulberry leaves to the silkworms until the silkworms spin silk cocoons; and (5) collecting the silk, so as to obtain mulberry silk that fluoresces under near-infrared light. The present invention selects upconversion nanoparticles capable of emitting fluorescence under the irradiation of near-infrared light which has stronger penetration, thus has better application in deep tissue imaging.

公开(公告)号：US20220084712A1

公开(公告)日：2022-03-17

申请号：US17297453

申请日：2020-05-05

申请人： The American University in Cairo

发明人： Nageh K. Allam ,  Basant A. Ali

IPC分类号： H01B1/12 ,  H01B13/00 ,  A01K67/04

摘要： A method for manufacturing an electrical circuit component includes preparing a mixture of a structured material and silkworm food. The method further includes feeding the mixture to at least one silkworm. The method further includes harvesting silk produced by the at least one silkworm, wherein the harvested silk includes at least one silkworm silk fiber including silkworm-digested portions of the structured material embedded in or on the at least one fiber. The method further includes incorporating the at least one fiber into an electrical circuit component.

公开(公告)号：US20210372007A1

公开(公告)日：2021-12-02

申请号：US16957990

申请日：2018-12-18

申请人： NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION ,  KOWA COMPANY, LTD.

发明人： Taiyo YOSHIOKA ,  Tsunenori KAMEDA ,  Akimune ASANUMA ,  Hironori SASSA

IPC分类号： D01B7/06 ,  A01K67/04 ,  D01C3/02

摘要： Developed and provided is a method of collecting a large amount of high-quality bagworm silk threads having no contaminant from bagworm nests in a convenient manner and at low cost. The habit of bagworms is utilized to allow a bagworm to build a nest using solvent-soluble substances or thermally meltable substances as nest materials, followed by dissolving or melting the nest materials to separate the nest material from the bagworm silk threads, whereby only pure bagworm silk threads constituting the bagworm nest can be obtained.

公开(公告)号：US20190185528A1

公开(公告)日：2019-06-20

申请号：US16275159

申请日：2019-02-13

申请人： University of Notre Dame du Lac ,  The University of Wyoming ,  Kraig Biocraft Laboratories, Inc.

发明人： Malcolm James Fraser ,  Randolph V. Lewis ,  Donald L. Jarvis ,  Kimberly Thompson ,  Joseph Hull ,  Yun-Gen Miao ,  Florence Teule ,  Bong-Hee Sohn ,  Young-Soo Kim

IPC分类号： C07K14/435 ,  A01K67/033 ,  A01K67/04

CPC分类号： C07K14/43586 ,  A01K67/0333 ,  A01K67/04 ,  A01K2217/052 ,  A01K2227/706 ,  A01K2267/01 ,  A01K2267/02 ,  A61K38/00 ,  C07K14/43518 ,  C07K2319/00

摘要： Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.

公开(公告)号：US20190153047A1

公开(公告)日：2019-05-23

申请号：US16246318

申请日：2019-01-11

申请人： University of Notre Dame du Lac ,  The University of Wyoming ,  Kraig Biocraft Laboratories, Inc.

发明人： Malcolm James Fraser, Jr. ,  Randolph V. Lewis ,  Donald L. Jarvis ,  Kimberly Thompson ,  Joseph Hull ,  Yun-Gen Miao ,  Florence Teulé ,  Bong-Hee Sohn ,  Young-Soo Kim

IPC分类号： C07K14/435 ,  A01K67/033 ,  A01K67/04

摘要： Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.

公开(公告)号：US20150322121A1

公开(公告)日：2015-11-12

申请号：US14754916

申请日：2015-06-30

申请人： THE UNIVERSITY OF NOTRE DAME ,  THE UNIVERSITY OF WYOMING ,  KRAIG BIOCRAFT LABORATORIES, INC.

发明人： Malcolm James FRASER ,  Randy LEWIS ,  Don JARVIS ,  Kimberly THOMPSON ,  Joseph HULL ,  Yun-Gen MAO ,  Florence TEULE ,  Bonghee SOHN ,  Youngsoo KIM

IPC分类号： C07K14/435 ,  A01K67/04

CPC分类号： C07K14/43586 ,  A01K67/0333 ,  A01K67/04 ,  A01K2217/052 ,  A01K2227/706 ,  A01K2267/01 ,  A01K2267/02 ,  A61K38/00 ,  C07K14/43518 ,  C07K2319/00

摘要： Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.

摘要翻译： 公开了包含编码包含一种或多种蜘蛛丝弹性和强度基序的嵌合丝多肽的至少一种核酸的转基因蚕。 还公开了包含编码多种嵌合蜘蛛丝多肽（Spider 2，Spider 4，Spider 6，Spider 8）的核酸的表达盒。 使用piggyBac载体系统将编码嵌合蜘蛛丝多肽的核酸掺入突变家蚕中以产生稳定的转基因蚕。 还提供了与天然蚕丝纤维相比具有改善的拉伸强度和弹性特征的嵌合丝纤维。 转基因家蚕大大促进了适用于各种医疗和工业应用的嵌合蚕丝纤维的商业生产。

公开(公告)号：US20150101541A1

公开(公告)日：2015-04-16

申请号：US14579966

申请日：2014-12-22

申请人： Sadia "Ross" Barrameda

发明人： Sadia "Ross" Barrameda

IPC分类号： A01K67/04 ,  C12N15/82

CPC分类号： C12N15/90 ,  A01K67/04 ,  A01K2207/25 ,  A01K2227/706 ,  A01K2267/02 ,  C12N15/8257

摘要： A method of producing new types of hybrid silk and hemp fibers is described, comprising the steps of inserting hemp genes into mulberry DNA within mulberry cells, and vice versa, to form genetically-altered mulberry and hemp cells; growing the genetically altered cells into a plant; feeding the genetically altered plant to silkworms; and the silkworms producing silk, wherein the silk has hemp characteristics. Another method of producing new types of hybrid silk and hemp fibers is described, comprising the steps of creating a bioreactor containing man-made silkworms, wherein the silkworms imitate the process of spinning a cocoon; artificially feeding the silkworms a hemp food source; and allowing the silkworms to produce silk, wherein the silk has hemp characteristics.

摘要翻译： 描述了生产新型杂交丝和大麻纤维的方法，包括将大麻基因插入桑树细胞内的桑树DNA中，反之亦然，形成遗传修饰的桑树和大麻细胞; 将遗传改变的细胞生长成植物; 将转基因植物喂养到家蚕; 丝绸生产丝绸，其中丝绸有大麻特征。 描述了生产新型杂化丝和大麻纤维的另一种方法，其包括以下步骤：产生含有人造蚕的生物反应器，其中所述蚕仿效纺丝茧的过程; 人工喂养蚕食大麻食物来源; 并允许蚕生产丝绸，其中丝绸具有大麻特征。

公开(公告)号：US20140189899A1

公开(公告)日：2014-07-03

申请号：US13694762

申请日：2013-01-03

申请人： Sadia "Ross" Barrameda

发明人： Sadia "Ross" Barrameda

IPC分类号： A01K67/04

CPC分类号： C12N15/90 ,  A01K67/04 ,  A01K2207/25 ,  A01K2227/706 ,  A01K2267/02 ,  C12N15/8257

摘要： The present invention provides a new and improved method for producing hybrid silk and like fibers. The invention provides a method of sequence of use which has many advantages over prior art.

摘要翻译： 本发明提供了一种用于生产杂化丝状纤维的新的和改进的方法。 本发明提供了一种与现有技术相比具有许多优点的使用顺序方法。

公开(公告)号：US20130281668A1

公开(公告)日：2013-10-24

申请号：US13919200

申请日：2013-06-17

申请人： Fritz Vollrath ,  Alexander Woods

发明人： Fritz Vollrath ,  Alexander Woods

IPC分类号： D01B7/00 ,  D01B7/04 ,  D01B9/00 ,  A01K67/04

CPC分类号： D01B7/00 ,  A01K67/04 ,  C07K14/43586 ,  D01B7/04 ,  D01B9/00 ,  D01D5/00 ,  D01D5/12 ,  D01F4/02

摘要： The present disclosure relates to a method and use for immobilising Lepidopteran larvae and a method, device and use for forced silking from an immobilised Lepidopteran larva. A method for immobilising Lepidopteran larvae is disclosed which comprises paralysing the Lepidopteran larvae by at least one of transgenically expressing a polypeptide having paralytic effects in the Lepidopteran larvae, injecting hemolymph, wherein the hemolymph is drawn from Lepidopteran larvae and oxidised prior to the injecting, or injecting a natural or artificial polypeptide having paralytic effects.

摘要翻译： 本公开内容涉及用于固定鳞翅目幼虫的方法和用途，以及用于从固定的鳞翅目幼虫强制丝锥的方法，装置和用途。 公开了一种固定鳞翅目幼虫的方法，其包括通过在鳞翅目幼虫中转基因表达具有麻痹作用的多肽，注射血淋巴中的至少一种来瘫痪鳞翅目幼虫，其中血淋巴从鳞翅目幼虫中抽出并在注射之前被氧化，或 注射具有麻痹作用的天然或人造多肽。

公开(公告)号：US20050139161A1

公开(公告)日：2005-06-30

申请号：US10711284

申请日：2004-09-08

申请人： Wendy Welshans

发明人： Wendy Welshans

IPC分类号： A01K20060101 ,  A01K67/033 ,  A01K67/04

CPC分类号： A01K67/04

摘要： A method and a housing assembly (10) for farming members of the Phylum Arthropoda (12) to collect silk therefrom in a centralized location without the members exhibiting territoriality tendencies is disclosed. The housing assembly (10) includes a wall (22) defining a plurality of frames (24) in close proximity with one another and disposed about a work space (26). Each frame (24) presents an open frame space (28) having a predetermined area and the work space (26) has a foot print (30) of a predetermined area such that a ratio is established therebetween. The ratio of the predetermined area of the open frame space (28) to the predetermined area of the foot print (30) is at least 1:5. A roof (32) is supported by the wall (22) for covering the frames (24) and the work space (26) and has an eave (34) that extends perpendicularly beyond the wall (22) a predetermined distance to protect the frames (24).

摘要翻译： 公开了一种用于在节肢动物（12）的耕作成员的方法和壳体组件（10），用于在集中的位置收集丝绸，而没有展示领土倾向的构件。 壳体组件（10）包括限定多个框架（24）的壁，所述多个框架彼此紧密邻近并且围绕工作空间（26）设置。 每个框架（24）呈现具有预定区域的开放框架空间（28），并且工作空间（26）具有预定区域的脚印（30），使得它们之间建立一个比例。 开放框架空间（28）的预定面积与脚印（30）的预定区域的比例至少为1:5。 屋顶（32）由壁（22）支撑，用于覆盖框架（24）和工作空间（26），并且具有以预定距离垂直地延伸超过壁（22）的檐（34），以保护框架 （24）。