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1155 条记录 (耗时 0.033 秒)

    Coenzyme-binding glucose dehydrogenase
    3.
    发明授权
    Coenzyme-binding glucose dehydrogenase 有权

    公开(公告)号：US11345897B2

    公开(公告)日：2022-05-31

    申请号：US16278025

    申请日：2019-02-15

    申请人： Ikeda Food Research Co., Ltd. ,  PHC Corporation

    发明人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Tetsunari Morita ,  Mika Kuyama ,  Tokuji Ikeda ,  Kenji Kano ,  Seiya Tsujimura

    IPC分类号： C12N9/02 ,  C12Q1/32 ,  C12Q1/54 ,  A61K38/00

    摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.

    Flavin-binding glucose dehydrogenase variant
    9.
    发明授权
    Flavin-binding glucose dehydrogenase variant 有权

    公开(公告)号：US11066690B2

    公开(公告)日：2021-07-20

    申请号：US16099779

    申请日：2017-05-09

    申请人： KIKKOMAN CORPORATION

    发明人： Yousuke Masakari ,  Chiaki Hara

    IPC分类号： C12Q1/00 ,  C12Q1/32 ,  C12N9/04 ,  C12N5/10 ,  C12N15/09 ,  C12M1/40 ,  C12N15/52

    摘要： The present invention relates to FAD-GDH consisting of the amino acid sequence of SEQ ID NO: 1 or 3, an amino acid sequence having an identity of 70% or more with the amino acid sequence of SEQ ID NO: 1 or 3, or an amino acid sequence having a deletion, substitution or addition of one or several amino acids in the amino acid sequence (the amino acid sequence of SEQ ID NO: 1 or the amino acid sequence having an identity of 70% or more with the amino acid sequence of SEQ ID NO: 1); having an amino acid substitution at the position(s) corresponding to the following amino acid(s): the amino acid at the 175th position in the amino acid sequence of SEQ ID NO: 1, the amino acid at the 214th position in the amino acid sequence of SEQ ID NO: 1, the amino acid at the 192nd position in the amino acid sequence of SEQ ID NO: 1, the amino acid at the 212th position in the amino acid sequence of SEQ ID NO: 1, the amino acid at the 218th position in the amino acid sequence of SEQ ID NO: 1, and/or the amino acid at the 226th position in the amino acid sequence of SEQ ID NO: 1; and having an improved thermal stability compared to that before the substitution, provided that an FAD-GDH consisting of the amino acid sequence of SEQ ID NO: 28, 30 or 32 is excluded.

    Glucose dehydrogenase
    10.
    发明授权
    Glucose dehydrogenase 有权

    公开(公告)号：US11046935B2

    公开(公告)日：2021-06-29

    申请号：US15772661

    申请日：2016-10-26

    申请人： Amano Enzyme Inc.

    发明人： Kyoichi Nishio ,  Yuzo Kojima ,  Shotaro Yamaguchi

    IPC分类号： C12N9/04 ,  C12N1/15 ,  C12N15/53 ,  C12Q1/54 ,  C12M1/34 ,  C12N15/09 ,  G01N33/66 ,  C12N5/10 ,  C12Q1/32

    摘要： Intended is to provide a highly practical novel FAD-GDH. A glucose dehydrogenase having the following properties is provided: (1) action: catalyzes the reaction of oxidizing hydroxyl groups of glucose to form glucono-δ-lactone in the presence of an electron acceptor; (2) substrate specificity: reactivity to D-xylose is 10% or less when the reactivity to D-glucose is 100%; (3) pH stability: stable at pH 5 to 8; (4) amino acid sequence: including the amino acid sequence set forth in SEQ ID NO: 1, or an amino acid sequence with an identity of 83% or more to the amino acid sequence.