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    METHOD FOR MAKING A PHYSICAL MAP OF A POPULATION OF BARCODED PARTICLES
    1.
    发明公开
    METHOD FOR MAKING A PHYSICAL MAP OF A POPULATION OF BARCODED PARTICLES 审中-公开

    公开(公告)号：US20240336959A1

    公开(公告)日：2024-10-10

    申请号：US18034595

    申请日：2021-12-17

    申请人： PIXELGEN TECHNOLOGIES AB

    发明人： Simon FREDRIKSSON ,  Filip KARLSSON

    IPC分类号： C12Q1/6841 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6876

    CPC分类号： C12Q1/6841 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6876

    摘要： Provided herein is a method for making a physical map of a population of barcoded particles. In some embodiments, the method may involve: producing a complex comprising: i. a population of barcoded particles, wherein the barcoded particles are uniquely barcoded by surface-tethered oligonucleotides that have unique particle identifier sequences; and ii. a population of bridging moieties that comprises oligonucleotide sequences; wherein the bridging moieties are hybridized directly or indirectly to complementary sites in the surface-tethered oligonucleotides; performing a ligation, polymerization and/or a gap-fill/ligation reaction on the complex, thereby producing reaction products that comprise pairs of unique particle identifier sequences or complements thereof from adjacent barcoded particles: sequencing the reaction products, analyzing the sequences to making one or more physical maps of the barcoded particles. Systems for practicing the method are also provided.

    SPATIAL ANALYSIS OF A PLANAR BIOLOGICAL SAMPLE
    2.
    发明公开
    SPATIAL ANALYSIS OF A PLANAR BIOLOGICAL SAMPLE 审中-公开

    公开(公告)号：US20240287587A1

    公开(公告)日：2024-08-29

    申请号：US18569594

    申请日：2022-06-23

    申请人： MOLECULENT AB

    发明人： Olof John ERICSSON

    IPC分类号： C12Q1/6841 ,  C12Q1/6806

    CPC分类号： C12Q1/6841 ,  C12Q1/6806

    摘要： Provided herein, among other things, is method for analyzing a planar biological sample. In some embodiments, the method may comprise: contacting an oligonucleotide or a conjugate comprising the same with a planar biological sample under conditions by which the oligonucleotide or conjugate specifically binds to sites in or on the sample; performing one or more steps to release and/or extend the oligonucleotide in situ, to produce a reporter probe; transferring the reporter probe from the sample to a planar support that does not comprise an array of oligonucleotides, in a way that preserves the spatial relationship of the reporter probe in the sample; and detecting the reporter probe on the support.

    METHODS FOR ASSESSING SAMPLE QUALITY PRIOR TO SPATIAL ANALYSIS USING TEMPLATED LIGATION
    4.
    发明公开
    METHODS FOR ASSESSING SAMPLE QUALITY PRIOR TO SPATIAL ANALYSIS USING TEMPLATED LIGATION 审中-公开

    公开(公告)号：US20240218432A1

    公开(公告)日：2024-07-04

    申请号：US18287560

    申请日：2022-04-20

    申请人： 10x Genomics, Inc.

    发明人： Paulius Mielinis

    IPC分类号： C12Q1/6841

    CPC分类号： C12Q1/6841

    摘要： Provided herein are methods of testing a biological sample for efficacy of detection of a target nucleic acid where the method includes RNA templated ligation including generating a sequence that is complementary to the hybridized ligation product that includes labelled nucleotides and detecting a signal corresponding to the ligation product on the substrate, thereby determining the efficacy of detection of the target nucleic acid.