公开(公告)号：US11981967B2

公开(公告)日：2024-05-14

申请号：US16858489

申请日：2020-04-24

申请人： SANGAMO THERAPEUTICS, INC.

发明人： Kyle McGovern ,  David S. Ojala

IPC分类号： C12N15/86 ,  A61K48/00 ,  C07K7/08 ,  C07K14/005 ,  C12N7/00 ,  C12N7/01 ,  C12Q1/6897 ,  C40B20/04

CPC分类号： C12Q1/6897 ,  C07K7/08 ,  C12N15/86 ,  C40B20/04 ,  C12N2750/14111 ,  C12Q2563/185

摘要： The present disclosure provides methods and compositions to develop AAV capsids with a desired characteristic compared to a natural AAV serotype. These capsids are useful, for example, for the delivery of genome engineering molecules and gene therapy molecules for the treatment of a subject in need thereof.

公开(公告)号：US11802281B2

公开(公告)日：2023-10-31

申请号：US16090829

申请日：2017-03-17

申请人： ETH ZURICH

发明人： Sai Reddy ,  William Kelton ,  Cristina Parola ,  Derek Mason ,  Mark Pogson

IPC分类号： C40B20/04 ,  C12N15/10 ,  C12N5/0781 ,  C12N9/22 ,  C12N15/113 ,  C40B10/00 ,  C40B40/08

CPC分类号： C12N15/1037 ,  C12N5/0635 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1058 ,  C12N15/1082 ,  C12N15/1086 ,  C12N15/113 ,  C40B10/00 ,  C40B20/04 ,  C40B40/08 ,  C12N15/1034 ,  C12N2310/20 ,  C12N2510/00 ,  C12N2510/02 ,  C12N2800/80 ,  C12Q2521/539 ,  C12N15/102 ,  C12Q2521/539

摘要： According to a first aspect of the invention, a method for the generation of a cell line is provided, comprising the steps of (a) providing a plurality of mammalian B cells, wherein each of the plurality of B cells comprises a transgenic genomic DNA sequence encoding a marker protein inserted into an endogenous immunoglobulin locus comprised in said B cell, and wherein the transgenic genomic DNA sequence is amenable to cleavage by a site directed nuclease, particularly Cas9; (b) replacing the transgenic genomic DNA sequence encoding a marker protein with a second transgenic DNA sequence encoding a protein of interest; (c) sorting B cells based on the presence or absence of the marker protein; and (d) collecting B cells in which the marker protein is absent.

公开(公告)号：US11713457B2

公开(公告)日：2023-08-01

申请号：US17986764

申请日：2022-11-14

申请人： 10x Genomics, Inc.

发明人： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC分类号： C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6874 ,  C12N15/10 ,  C40B50/16 ,  C40B20/04

CPC分类号： C12N15/1065 ,  C12Q1/683 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B20/04 ,  C40B50/16 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/149 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20230235482A1

公开(公告)日：2023-07-27

申请号：US18072187

申请日：2022-11-30

申请人： Inscripta, Inc.

发明人： Richard FOX ,  Daniel HELD

IPC分类号： C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

CPC分类号： C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/2809 ,  C07K14/70539 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/907 ,  C40B20/04 ,  C40B40/02 ,  C12N15/1037

摘要： The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US11667951B2

公开(公告)日：2023-06-06

申请号：US16344004

申请日：2017-10-24

申请人： GENEINFOSEC, INC.

发明人： Sterling Sawaya

IPC分类号： C12Q1/6806 ,  C40B70/00 ,  C40B20/04 ,  C12N15/10 ,  C12N15/66

CPC分类号： C12Q1/6806 ,  C12N15/10 ,  C12N15/66 ,  C40B70/00 ,  C40B20/04 ,  C12Q1/6806 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/161 ,  C12Q2563/179 ,  C12Q2565/514

摘要： Methods related to concealment of genetic information present within nucleic acid sequences, wherein individual nucleic acid molecules are barcoded. In some embodiments barcoding occurs before, after, or during enrichment. Barcoded nucleic acids are then combined with control barcoded nucleic acids. Different methods are provided for barcoding and pooling to conceal different types of genetic information present within nucleic acids.

公开(公告)号：US11629344B2

公开(公告)日：2023-04-18

申请号：US17860880

申请日：2022-07-08

申请人： 10x Genomics, Inc.

发明人： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC分类号： C12Q1/6874 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12N15/10 ,  C40B50/16 ,  C40B20/04

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US11608570B2

公开(公告)日：2023-03-21

申请号：US16321027

申请日：2017-07-31

申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften E.V.

发明人： David Ng ,  Oliver Griesbeck ,  Mutlu Erdogan

IPC分类号： C40B30/06 ,  C12N15/90 ,  C12N15/10 ,  C12N9/22 ,  C40B20/04 ,  C40B50/06 ,  C40B70/00

摘要： The present invention relates to a method for producing a panel of cells (i.e. a cell library) expressing various different mutant variants of a protein of interest, wherein only one of said mutant variants is expressed per cell from a single gene copy. The present invention also relates to a method or cell library for identifying a mutant variant of a protein of interest having a different or modified biological activity as compared to the corresponding wild-type protein of interest. According to the present invention the identified mutant variant of a protein of interest may be applied for white biotechnology.

公开(公告)号：US11474107B2

公开(公告)日：2022-10-18

申请号：US17696631

申请日：2022-03-16

申请人： Apton Biosystems, Inc.

发明人： Bryan P. Staker ,  Niandong Liu ,  Bart Lee Staker ,  Michael David McLaughlin

IPC分类号： G01N33/58 ,  G01N27/414 ,  C12Q1/6837 ,  C12Q1/6825 ,  C12Q1/6804 ,  C40B20/04

摘要： Electrical detection methods are used to identify and further characterize single-molecule target analytes such as proteins and nucleic acids. A composition including a probe region and a tail region is contacted with a target analyte. The probe region specifically binds to the target analyte. The tail region is coupled to the probe region, and includes a nucleic acid template for polynucleotide synthesis. When conditions are such that polynucleotide synthesis occurs along the tail region, one hydrogen ion is released for every nucleotide that is incorporated into the tail region. A transistor such as an ISFET detects and measures changes in ion concentration, and these measurements can be used to identify the tail region and thus characterize the corresponding target analyte.

公开(公告)号：US20220243361A1

公开(公告)日：2022-08-04

申请号：US17728135

申请日：2022-04-25

申请人： Inscripta, Inc.

发明人： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC分类号： C40B50/06 ,  C12N15/90 ,  C12N15/10 ,  C40B40/10 ,  C12N15/85 ,  C07K16/28 ,  C40B30/04 ,  C40B40/02 ,  C07K14/74 ,  C12N15/81 ,  C12N9/22 ,  C40B20/04 ,  C40B70/00

摘要： The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US20220155316A1

公开(公告)日：2022-05-19

申请号：US17588754

申请日：2022-01-31

申请人： THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO

发明人： ANDREW EMILI

IPC分类号： G01N33/68 ,  C07D493/10 ,  C40B20/04

摘要： Described are optical methods and reagents for sequencing polypeptides. A probe that exhibits different spectral properties when conjugated to different N-terminal amino acids is conjugated to the N-terminal amino acid of a polypeptide. Sequentially detecting one or more spectral properties of the probe conjugated to the N-terminal amino acid and cleaving the N-terminal amino acid produces sequence information of the polypeptide. The use of super-resolution microscopy allows for the massively parallel sequencing of individual polypeptide molecules in situ such as within a cell. Also described are probes comprising hydroxymethyl rhodamine green, an isothiocyanate group and a protecting group.