公开(公告)号：US20240109003A1

公开(公告)日：2024-04-04

申请号：US17768671

申请日：2020-11-19

申请人： PURIDIFY LTD

发明人： Lennart Sörby

IPC分类号： B01D15/24 ,  B01D15/38 ,  B01D15/42 ,  G01N30/82

CPC分类号： B01D15/247 ,  B01D15/3804 ,  B01D15/424 ,  G01N30/82

摘要： The present invention relates to a method for controlling fraction collection of a target product 27 in a chromatography system 49 configured for cyclic purification performed on a sample comprising the target product 27. The method comprises determining trigger points for target product 27 collection in relation to presence of target product in the outlet; setting a first time period based on the trigger points within which the elution in the first cycle is captured; evaluating timing of the captured elution to identify the next time period; applying the timing to capture elution during the elution phase in the following cycle; and collecting the captured elution in the following cycle. The last three steps are repeated to capture the elution during the elution phase of the following cycles until no more target product 27 is desired.

公开(公告)号：US20220003722A1

公开(公告)日：2022-01-06

申请号：US17282764

申请日：2018-10-15

申请人： SHIMADZU CORPORATION

发明人： Satoru WATANABE

IPC分类号： G01N30/32 ,  G01N30/82

摘要： In a chromatograph, a mobile phase is supplied to any of a plurality of columns by a mobile phase supplier, and a sample is supplied to the column by a sample supplier. The sample that has passed through the column is detected by a detector. In a chromatographic control device, a flow rate of the mobile phase for the analysis with use of the column is registered by a flow rate registrar in association with column specifying information for specifying each column. The flow rate of the mobile phase registered in association with a selected column is acquired from registered content of the flow rate registrar. The chromatograph is controlled such that the analysis is performed by an analysis controller while the mobile phase is supplied to the selected column with use of the selected column and the flow rate of the mobile phase acquired by a flow rate acquirer.

公开(公告)号：US20210349062A1

公开(公告)日：2021-11-11

申请号：US17284949

申请日：2019-09-20

申请人： SHIMADZU CORPORATION

发明人： Tomohiro SHAGAWA ,  Saki YOSHINO ,  Iori OTOMO

IPC分类号： G01N30/82 ,  G01N30/86

摘要： A liquid chromatography separation system includes a separator, a collector, a switch valve and a collection controller. The collector has at least one sample loop and connects the sample loop to a position farther downstream than the separator and collect an eluate including a desired sample component among sample components obtained by separation in the separator in the sample loop. The switch valve switches between a state in which the separator is connected the sample loop and a state in which the separator is not connected to the sample loop. The collection controller is configured to control an operation of switching the switch valve and collect a predetermined amount, which is equal to or smaller than a volume of the sample loop, of an eluate including a desired sample component obtained by separation in the separator in the sample loop from a peak-beginning point of the sample component.

公开(公告)号：US20210055268A1

公开(公告)日：2021-02-25

申请号：US17045756

申请日：2019-04-05

申请人： Agilent Technologies, Inc.

发明人： Stephan Buckenmaier ,  Konstantin Shoykhet

IPC分类号： G01N30/20 ,  G01N30/46 ,  G01N30/82

摘要： A sampling unit for handling a sample fluid includes a sample container having a length and being configured for receiving and storing the sample fluid, and a sample segment dispatching unit configured for providing a plurality of individual sample packages of the fluidic sample, each contained in a respective volume segment along the length of the sample container, and for individually dispatching each of the plurality of individual sample packages for further processing in a fluid processing unit.

公开(公告)号：US10830739B2

公开(公告)日：2020-11-10

申请号：US15500706

申请日：2015-05-28

申请人： Agilent Technologies, Inc.

发明人： Herbert Anderer ,  Konstantin Shoykhet ,  Klaus Witt ,  Stephan Buckenmaier

IPC分类号： G01N30/46 ,  G01N30/82 ,  G01N30/22 ,  G01N30/24 ,  B01D15/18 ,  B01D15/24 ,  G01N30/44 ,  G01N30/20 ,  G01N30/84 ,  G01N30/02

摘要： In a method for processing successive fluidic sample portions provided by a sample source, sample reception volumes are filled successively temporarily with at least a respective one of the sample sections, and the sample sections are emptied successively out of the sample reception volumes in such a way, that, while emptying, it is avoided to bring two respective ones of the sample sections, which have not left the sample source directly adjacent to one another, in contact with one another.

公开(公告)号：US20200064315A1

公开(公告)日：2020-02-27

申请号：US16485974

申请日：2017-03-07

申请人： Shimadzu Corporation

发明人： Soichiro TAMAOKI ,  Tsutomu OKOBA ,  Takayuki IRIKI ,  Shiori UEDA

IPC分类号： G01N30/82 ,  G01N30/88 ,  G01N30/86 ,  B01D15/24

摘要： A fraction collector controller includes a threshold storing part, a peak detecting part, a number-of-points-of-data setting part, and a control part. The peak detecting part retrieves, at regular intervals, signals that are from a detector of a liquid chromatograph and are used as points of data, and detects a start point and an end point of a peak by comparing a signal strength and/or an inclination of each of the points of data with the threshold(s). The number-of-points-of-data setting part is configured to set a number of the points of data with which the peak detecting part compares the threshold(s) to detect a start point and an end point of a peak.

公开(公告)号：US10391424B2

公开(公告)日：2019-08-27

申请号：US15072618

申请日：2016-03-17

申请人： SHIMADZU CORPORATION

发明人： Tsutomu Okoba ,  Takayuki Iriki

IPC分类号： B01D15/24 ,  G01N30/80 ,  G01N30/82 ,  B01D15/10

摘要： A moving section that moves in the horizontal plane direction at above a collection container section where collection containers are set is provided with a nozzle holding section for holding a nozzle, and a waste port holding section for holding a waste port. The moving section is capable of moving the nozzle holding section or the waste port holding section. Accordingly, one of a state where the waste port is at a position immediately below the tip of the nozzle and a state where the waste port is not at the position immediately below the tip of the nozzle may be achieved.

公开(公告)号：US10376814B2

公开(公告)日：2019-08-13

申请号：US15542925

申请日：2015-01-30

申请人： Agilent Technologies, Inc.

发明人： Edwin E. Wikfors

IPC分类号： B01D1/14 ,  B04B7/00 ,  B01D11/02 ,  B01D15/24 ,  B01D15/40 ,  B01D19/00 ,  B01D46/00 ,  B04B11/06 ,  G01N30/82 ,  G01N30/84 ,  G01N30/88

摘要： A separator (e.g. an impact centrifugal separator) is for use in collection of liquid portions of a bi-phasic flowstream in a supercritical fluid system. A separator chamber defines an interior space, surrounded by a spiral channel and that has an exit at a lower portion of the separator chamber. A flowstream director (e.g. an entry lube) focuses the flowstream so that the flowstream impacts a wail of the spiral channel at an angle that promotes coalescence of the liquid component. The spiral channel may promote further coalescence of the liquid portions within the constraining spiral channel after impact. A central director may be included in the interior space of the separator chamber to promote flow of the gaseous component toward the exit. A dripper may be included in fluid communication with the spiral channel, for example, in the exit and to direct the coalesced liquid into a collection vessel.

公开(公告)号：US20190017974A1

公开(公告)日：2019-01-17

申请号：US16070100

申请日：2016-10-31

申请人： KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

发明人： Sang-Won LEE ,  Hangyeore LEE

IPC分类号： G01N30/34 ,  G01N30/82 ,  G01N30/89 ,  B01D15/08

摘要： Disclosed are non-continuous sample fractionating and concatenating device and a dual online multidimensional liquid chromatography system having the same. The non-continuous sample fractionating and concatenating device according to an embodiment of the present disclosure includes a sample supply module which supplies a sample to be analyzed, and a sample fractionation module connected to the sample supply module, and which is continuously supplied with the sample, sets a plurality of unit sample supply times obtained by equally dividing a total sample supply time during which the sample is supplied from the sample supply module, sets a plurality of unit fractionation intervals obtained by equally dividing each of the plurality of unit sample supply times, and concatenates and stores the sample supplied during corresponding unit fractionation intervals within each unit sample supply time to acquire a plurality of fractions.

公开(公告)号：US09535042B2

公开(公告)日：2017-01-03

申请号：US14789037

申请日：2015-07-01

申请人： Microsaic Systems Plc

发明人： Steven Wright ,  Alexander Iain McIntosh

IPC分类号： G01N30/72 ,  G01N33/487 ,  G01N30/82 ,  H01J49/16 ,  G01N1/40 ,  G01N30/88 ,  G01N30/60 ,  H01J49/00 ,  H01J49/04

CPC分类号： G01N30/7233 ,  G01N30/6095 ,  G01N30/7266 ,  G01N30/82 ,  G01N33/487 ,  G01N2001/4016 ,  G01N2030/8813 ,  G01N2030/8881 ,  H01J49/0031 ,  H01J49/0431 ,  H01J49/165

摘要： Monitoring biomolecule separations by mass spectrometry is described. The mixture to be separated is introduced into a first fluidic stream, which then passes through a chromatography column. Small samples are periodically taken from the first fluidic stream as it leaves the chromatography column and injected into a first branch of a second fluidic stream. Low molecular weight components detrimental to the efficient operation of the mass spectrometer are removed by an in-line dialysis cell. A second fraction of the second fluidic stream acts as the dialysate. In a second and preferred system provided in accordance with the present teaching, the first fraction of the second fluidic stream is further split downstream of the sampling mechanism through the use of a three-way connector. Approximately 0.3 to 5 microliters per minute continues through the dialysis cell and thereafter to the electrospray emitter of the mass spectrometer.

摘要翻译： 描述了通过质谱监测生物分子分离。 将待分离的混合物引入第一流体流中，然后通过色谱柱。 当离开色谱柱并将其注入第二流体流的第一分支时，从第一流体流中周期性地取出小样品。 通过在线透析池除去对质谱仪的有效操作有害的低分子量组分。 第二流体流的第二部分用作透析液。 在根据本教导提供的第二和优选系统中，第二流体流的第一部分通过使用三通连接器进一步分离在采样机构的下游。 每分钟约0.3至5微升继续通过透析池，此后进入质谱仪的电喷雾发射器。