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171 条记录 (耗时 0.03 秒)

    METHODS OF ESTIMATING BLOOD GLUCOSE AND RELATED SYSTEMS
    1.
    发明申请
    METHODS OF ESTIMATING BLOOD GLUCOSE AND RELATED SYSTEMS 审中-公开

    公开(公告)号：US20180364262A1

    公开(公告)日：2018-12-20

    申请号：US16061951

    申请日：2016-12-15

    申请人： The General Hospital Corporation

    发明人： Roy Malka ,  John M. Higgins ,  David M. Nathan

    IPC分类号： G01N33/72 ,  G01N33/555

    摘要： A method includes estimating a value of a parameter indicative of an age or lifespan of a population of red blood cells of a subject, estimating a value of average glucose (AG) of the subject based on (i) the value of the parameter and (ii) a value indicative of an amount of glycated hemoglobin (HbA1c) of the subject, and providing information for treatment or diagnosis of a hyperglycemia condition of the subject based on the estimated value of AG.

    ENGINEERED RED BLOOD CELL-BASED BIOSENSORS
    2.
    发明申请
    ENGINEERED RED BLOOD CELL-BASED BIOSENSORS 审中-公开

    公开(公告)号：US20180246092A1

    公开(公告)日：2018-08-30

    申请号：US15908077

    申请日：2018-02-28

    申请人： Northwestern University

    发明人： Joshua N. Leonard ,  Kelly A. Schwarz ,  Taylor B. Dolberg

    IPC分类号： G01N33/555 ,  G01N21/64 ,  G01N33/566

    CPC分类号： G01N33/555 ,  C12N5/0641 ,  C12N2510/00 ,  G01N21/6428 ,  G01N33/566 ,  G01N33/80

    摘要： Disclosed are systems and methods for detecting extracellular ligands. The disclosed systems and method for detecting extracellular ligands typically comprise or utilize engineered red blood cells (eRBCs) that comprises modular extracellular sensors. The eRBCs may comprise: (i) a first exogenous extracellular sensor; the first extracellular sensor comprising: a) a ligand binding domain, b) a transmembrane domain, and c) a first fragment of a functional protein, and (ii) a second exogenous extracellular sensor; the second extracellular sensor comprising: a) a ligand binding domain, b) a transmembrane domain, and c) a second fragment of the functional protein. In the eRBCs, the ligand binding domain of the first exogenous sensor and the ligand binding domain of the second exogenous sensor bind to the same ligand to form a ternary complex, and the first fragment of the functional protein and the second fragment of the functional protein interact in the ternary complex to reconstitute functional activity of the functional protein.

    Method of lysis of erythrocytes
    4.
    发明授权
    Method of lysis of erythrocytes 有权
    红细胞裂解方法

    公开(公告)号：US07678583B2

    公开(公告)日：2010-03-16

    申请号：US11868580

    申请日：2007-10-08

    申请人： Andre van Agthoven ,  Jean-Pierre Daziano ,  John Allen Maples

    发明人： Andre van Agthoven ,  Jean-Pierre Daziano ,  John Allen Maples

    IPC分类号： G01N33/555

    CPC分类号： C07D295/205 ,  G01N33/5002 ,  G01N33/5094 ,  Y10T436/107497 ,  Y10T436/25

    摘要： A multifunctional reagent for erythrocytes containing an amount sufficient to produce the lysis of erythrocytes or the sphering of erythrocytes in such a way that they can be detected by a cytometer or an automatic counting device, of a carbamate or of an agent inducing the formation by the erythrocytes, from carbonate and from a nitrogenated heterocycle or ammonium ions, of a carbamate combined with the absorption of CO2 by the erythrocytes, process for lysing or sphering erythrocytes and preparation process for leucocytes.

    摘要翻译： 一种用于红细胞的多功能试剂，其含有足以产生红细胞裂解或红细胞球形的量，使得它们可以通过血细胞计数器或自动计数装置，氨基甲酸酯或诱导由 来自碳酸盐和氮化杂环或铵离子的红细胞，与由红细胞吸收CO 2的氨基甲酸酯结合，红细胞裂解或穿孔过程以及白细胞的制备方法。

    Nucleic acid molecules correlated with the Rhesus weak D phenotype
    5.
    发明授权
    Nucleic acid molecules correlated with the Rhesus weak D phenotype 有权
    核酸分子与恒河猴弱D表型相关

    公开(公告)号：US07553629B2

    公开(公告)日：2009-06-30

    申请号：US11834513

    申请日：2007-08-06

    申请人： Willy A. Flegel ,  Franz F. Wagner

    发明人： Willy A. Flegel ,  Franz F. Wagner

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  G01N33/555 ,  G01N33/557 ,  G01N33/558 ,  G01N33/559 ,  G01N33/563

    CPC分类号： G01N33/80 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/172

    摘要： The present invention relates to novel nucleic acid molecules encoding a Rhesus D antigen contributing to the weak D phenotype which are characterized by one or a combination of missense mutations or by a gene conversion involving exons 6 to 9 of the RHD and RHCE genes. The present invention further relates to vectors comprising the nucleic acid molecules of the invention, to hosts transformed with said vectors, to proteins encoded by said nucleic acid molecules and to methods of producing such polypeptides. The fact that missense mutations and the conversion referred to above can be directly correlated to the weak D phenotype has a significant impact on the routine testing of blood samples. For example, oligonucleotides and antibodies can now be designed that generally allow the detection of weak D phenotypes in a sample. Such oligonucleotides, antibodies as well as a variety of diagnostic methods all fall within the scope of the present invention. RhD antigens encoded by the novel nucleic acid molecules may be used for the characterization, standardization and quality control of monoclonal and polyclonal anti-D antisera. Finally, the invention relates to a kit useful for testing for the presence of weak D phenotypes.

    摘要翻译： 本发明涉及编码有助于弱D表型的恒河猴D抗原的新型核酸分子，其特征在于一种或多种错义突变的组合或通过涉及RHD和RHCE基因的外显子6至9的基因转化。 本发明还涉及包含本发明的核酸分子的载体，用所述载体转化的宿主，由所述核酸分子编码的蛋白质和产生这种多肽的方法。 事实上，错义突变和上述转化可以与弱D表型直接相关，对血液样本的常规检测有显着影响。 例如，现在可以设计寡核苷酸和抗体，其通常允许检测样品中的弱D表型。 这样的寡核苷酸，抗体以及各种诊断方法都落入本发明的范围内。 由新型核酸分子编码的RhD抗原可用于单克隆抗体和多克隆抗-D抗血清的表征，标准化和质量控制。 最后，本发明涉及可用于测试弱D表型存在的试剂盒。

    Photochemically Amplified Bioassay
    6.
    发明申请
    Photochemically Amplified Bioassay 审中-公开
    光化学扩增生物测定

    公开(公告)号：US20090053694A1

    公开(公告)日：2009-02-26

    申请号：US11306378

    申请日：2005-12-26

    申请人： Leo B. Kriksunov

    发明人： Leo B. Kriksunov

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  G01N33/555

    CPC分类号： G01N33/54373 ,  B01J35/004

    摘要： In the present invention, a reagent capable of immunospecific reaction with the analyte of interest is conjugated to a photocatalytic microparticle. After the immunospecific binding has occurred, the assay amplification is performed by exposing photocatalytic particles to actinic UV light in presence of an oxidizable compound. Photocatalytic particles are catalyzing multiple occurrences of oxidation of oxidizable compound under UV light irradiation resulting in detectable changes such as color change. This provides for amplification of each single act of immunospecific binding and is followed by colormetric detection. Thus a high sensitivity quantitative or qualitative immunoassay can be realized.

    摘要翻译： 在本发明中，与感兴趣的分析物能够进行免疫特异性反应的试剂与光催化微粒共轭。 在发生免疫特异性结合之后，通过在可氧化化合物的存在下将光催化剂颗粒暴露于光化UV光下进行测定扩增。 光催化颗粒在紫外光照射下催化可氧化化合物的多次氧化，导致可检测的变化，例如颜色变化。 这提供了每个单独的免疫特异性结合作用的扩增，然后进行比色检测。 因此，可以实现高灵敏度定量或定性免疫测定。

    Methods and systems for detecting particles
    7.
    发明申请
    Methods and systems for detecting particles 审中-公开
    检测颗粒的方法和系统

    公开(公告)号：US20080003665A1

    公开(公告)日：2008-01-03

    申请号：US11480184

    申请日：2006-07-03

    申请人： Radislav Alexandrovich Potyrailo ,  Steven Francis LeBoeuf ,  Rui Chen

    发明人： Radislav Alexandrovich Potyrailo ,  Steven Francis LeBoeuf ,  Rui Chen

    IPC分类号： G01N33/555 ,  C12M3/00 ,  G01J3/45

    CPC分类号： G01N15/1459

    摘要： A system for detecting a particle disposed in a detection area. The system includes a light-emitting source for generating light. The light is directed at the particle. The system further includes a modulator configured to in-situ modulate at least one environmental parameter of the particle to alter a detectable response of the particle. The modulator provides an enhancement in detection selectivity of the particle in the presence of interfering particles and species. Further, the system includes a detector configured to detect alteration in the detectable response of the particle.

    摘要翻译： 一种用于检测设置在检测区域中的颗粒的系统。 该系统包括用于产生光的发光源。 光线指向颗粒。 该系统还包括配置成原位调节颗粒的至少一个环境参数以改变颗粒的可检测响应的调制器。 调节剂在干扰颗粒和物质的存在下提供了颗粒检测选择性的增强。 此外，该系统包括被配置成检测颗粒的可检测反应的改变的检测器。

    Composition for homogeneous multiplexed microparticle-based assay
    9.
    发明申请
    Composition for homogeneous multiplexed microparticle-based assay 审中-公开
    用于均相多元微粒检测的组合物

    公开(公告)号：US20060263836A1

    公开(公告)日：2006-11-23

    申请号：US11360072

    申请日：2006-02-23

    申请人： Mark Connelly ,  Mark Kopnitsky

    发明人： Mark Connelly ,  Mark Kopnitsky

    IPC分类号： G01N33/555

    CPC分类号： G01N33/54313 ,  Y10T436/10

    摘要： A method and composition for the purpose of detecting and measuring analytes, such as antibodies, which are capable of binding with certain binding partners such as antigens. A homogenous assay is performed in the presence of free unbound antibodies. Such a homogeneous assay testing for specific antibodies is herein possible by defining of test subsets of microparticles having specific antigens thereon which are capable of binding with specific target antibodies. The microparticle suspension also includes at least two calibration subsets of microparticles having a binding partner thereon with at least two known levels of concentration which is capable of binding with human antibodies for the purpose of assay calibration. A verification subset of microparticles is included with another binding partner thereon at a known concentration, capable of binding with anti-human antibodies. This suspension is incubated with a human sample and then is incubated with a tagging component which is measurable for calibration and verification and finally for determining the concentration of target antibodies bound to the test subsets. A separate verification subset of microparticles is included for verifying the calibration and for yielding a correction factor therefore. A complete calibration calculation is performed for each sample being tested. Preferably, the attaching component of the tagging component is the same molecule as the first binding partner material positioned on the calibration subsets of microparticles.

    摘要翻译： 用于检测和测量能够与某些结合配偶体如抗原结合的分析物（例如抗体）的方法和组合物。 在游离未结合的抗体存在下进行均一的测定。 通过在其上具有能够与特异性靶抗体结合的具有特异性抗原的微粒的测试子集来定义对特异性抗体的这种均一测定测试。 微粒悬浮液还包括具有至少两个已知浓度浓度的至少两个具有结合配偶体的微粒的校准子集，其能够与人抗体结合以进行测定校准。 微粒的验证子集与另一个结合配偶体以已知的浓度包含在其中，能够与抗人抗体结合。 将该悬浮液与人样品一起孵育，然后与标记组分一起孵育，标记组分可测量用于校准和验证，最后用于测定与测试子集结合的靶抗体的浓度。 包括微粒的单独验证子集，用于验证校准和因此产生校正因子。 对每个被测样品进行完整的校准计算。 优选地，标记组分的附着成分与位于微粒校准子集上的第一结合配对物质相同。