公开(公告)号：WO2005108620A3

公开(公告)日：2006-04-13

申请号：PCT/US2005015345

申请日：2005-05-03

Applicant: HANDYLAB INC ,  WU BETTY ,  ALTHAUS JOHN S ,  BRAHMASANDRA SUNDARESH N ,  HANDIQUE KALYAN ,  PHADKE NIKHIL

Inventor： WU BETTY ,  ALTHAUS JOHN S ,  BRAHMASANDRA SUNDARESH N ,  HANDIQUE KALYAN ,  PHADKE NIKHIL

IPC: B01F5/06 ,  B01F13/00 ,  B01L3/00 ,  B01L3/14 ,  B01L7/00 ,  C12N15/10 ,  C12Q1/68 ,  F16K99/00 ,  C12M1/34 ,  C12P19/34

CPC classification number: C12Q1/686 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0059 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/5082 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0442 ,  B01L2400/0677 ,  B01L2400/0683 ,  B01L2400/0694 ,  C12N15/1006 ,  C12Q1/6806 ,  F16K99/0001 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/0036 ,  F16K99/0044 ,  F16K2099/0084 ,  C12Q2565/629

Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.

Abstract translation: 公开了用于处理多核苷酸（例如DNA）的方法和系统。 处理区域包括用在第一组条件（例如温度和pH）下重新获得多核苷酸的配体修饰的一个或多个表面（例如，颗粒表面），并在第二组条件（例如较高的温度和/ 或更多的碱性pH）。 处理区域可用于例如浓缩样品的多核苷酸和/或来自多核苷酸的分离的扩增反应抑制剂。 公开了具有处理区域的微流体装置。

公开(公告)号：WO2007112114A2

公开(公告)日：2007-10-04

申请号：PCT/US2007007513

申请日：2007-03-26

Applicant: HANDYLAB INC ,  HANDIQUE KALYAN ,  BRAHMASANDRA SUNDARESH N ,  GANESAN KARTHIK ,  WU BETTY ,  PHADKE NIKHIL ,  PARUNAK GENE ,  WILLIAMS JEFF

Inventor： HANDIQUE KALYAN ,  BRAHMASANDRA SUNDARESH N ,  GANESAN KARTHIK ,  WU BETTY ,  PHADKE NIKHIL ,  PARUNAK GENE ,  WILLIAMS JEFF

IPC: C12M3/00

CPC classification number: B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/06 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/18 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/0442 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0611 ,  B01L2400/0677 ,  B01L2400/0683 ,  F16K99/0001 ,  F16K99/003 ,  F16K99/0032 ,  F16K99/0044 ,  F16K99/0061 ,  F16K2099/0084 ,  G01N2035/00881

Abstract: This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.

Abstract translation: 该专利申请描述了用于处理含有多核苷酸的样品并用于在其上提供诊断结果的集成设备。 该装置被配置为接收包含用于处理样品的试剂和网络的微流控盒。 还描述了使用该装置的方法。

公开(公告)号：WO2005011867A3

公开(公告)日：2005-04-21

申请号：PCT/US2004025181

申请日：2004-08-02

Applicant: HANDYLAB INC ,  WU BETTY ,  HANDIQUE KALYAN ,  PARUNAK GENE ,  KEHRER AARON ,  GANESAN KARTHIK

Inventor： WU BETTY ,  HANDIQUE KALYAN ,  PARUNAK GENE ,  KEHRER AARON ,  GANESAN KARTHIK

IPC: B01L3/00 ,  B01L99/00 ,  B81B1/00

CPC classification number: C12Q1/6806 ,  B01L3/502707 ,  B01L3/502738 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0677 ,  B33Y40/00 ,  B33Y80/00 ,  C12Q2523/109 ,  G01N1/40 ,  Y10T436/25 ,  Y10T436/25375

Abstract: A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.

Abstract translation: 微流体装置包括用于将含颗粒液体样品输入装置的输入口，保持构件和压力致动器。 保持构件与输入端口连通并且构造成将含颗粒液体样本的颗粒与含有流体样本的颗粒的液体的第一部分在空间上分离。 压力致动器将至少一些分离的颗粒与从颗粒分离的液体的第一部分的子集重新结合。 该装置还可以包括从保留部件接收颗粒和液体的裂解室。 裂解室热溶解颗粒以释放其内容物。

公开(公告)号：WO2007044917A3

公开(公告)日：2007-07-26

申请号：PCT/US2006040171

申请日：2006-10-11

Applicant: HANDYLAB INC ,  HANDIQUE KALYAN ,  WILLIAMS JEFF ,  BRAHMASANDRA SUNDARESH N ,  PHADKE NIKHIL ,  WU BETTY

Inventor： HANDIQUE KALYAN ,  WILLIAMS JEFF ,  BRAHMASANDRA SUNDARESH N ,  PHADKE NIKHIL ,  WU BETTY

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: B01L3/502715 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502738 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/0867 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/0487 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/0034 ,  F16K99/0044 ,  F16K99/0061 ,  F16K2099/0084 ,  G01N1/34 ,  G01N2035/00158

Abstract: Methods and systems for preparing polynucleotide samples are disclosed. The invention includes a microfluidic system for converting a sample containing one or more polynucleotides into a form suitable for analyzing the polynucleotides, comprising: a cartridge receiving element, an insertable and removable cartridge, a heating element configured to heat one or more regions of the cartridge, and control circuitry, wherein the insertable cartridge comprises: a microfluidic component that is configured to accept the sample and one or more reagents, and to react the sample and the reagents, in order to produce a prepared sample suitable for analyzing the one or more polynucleotides. The invention further comprises a multi-sample cartridge for converting a number of samples, each containing one or more polynucleotides, into respective forms suitable for analyzing the polynucleotides, comprising: at least a first microfluidic component and a second microfluidic component.

Abstract translation: 公开了制备多核苷酸样品的方法和系统。 本发明包括用于将含有一种或多种多核苷酸的样品转化成适于分析多核苷酸的形式的微流体系统，包括：盒接收元件，可插入和可移除的盒，加热元件，其配置成加热盒的一个或多个区域 以及控制电路，其中所述可插入筒包括：微流体组分，其被配置为接受所述样品和一种或多种试剂，并使所述样品和所述试剂反应，以便产生适于分析所述一种或多种 多核苷酸。 本发明还包括用于将多个样品（各自含有一个或多个多核苷酸）转化成适于分析多核苷酸的各自形式的多样品盒，包括：至少第一微流体组分和第二微流体组分。

公开(公告)号：WO03012406A9

公开(公告)日：2003-03-20

申请号：PCT/US0209440

申请日：2002-03-27

Applicant: HANDYLAB INC

Inventor： PARUNAK GENE ,  HANDIQUE KALYAN ,  WU BETTY ,  GANESAN KARTHIK

IPC: G11C16/06 ,  B01L3/00 ,  B81B1/00 ,  F04B19/00 ,  F04B19/24 ,  F04F1/06 ,  G01N21/29 ,  B01D57/02 ,  C02F1/40 ,  C12M1/34 ,  C12M1/36 ,  C12N13/00 ,  C12Q1/68

CPC classification number: B01L3/502738 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2400/0442

Abstract: The present invention relates to a system and method for moving samples, such as fluids, within a microfluidic system using a plurality of gas actuators (168, 170, 172) for applying pressure at different locations within the microfluidic. The system includes a substrate (130, 132) which forms a fluid network through which fluid flows, and a plurality of gas actuators (168, 170, 172) integral with the substrate (130, 132). One such actuator (168) is coupled to the network at a first location for providing gas pressure to move a microfluidic sample within the network. Another gas actuator (170) is coupled to the network at a second location for providing gas pressure to further move at least a portion of the microfluidic sample within the network. A valve (915, 216) is coupled to the microfluidic network so that, when the valve is closed, it substantially isolates the second gas actuator from the first gas actuator.

Abstract translation: 本发明涉及一种使用多个用于在微流体内的不同位置施加压力的气体致动器（168,170,172）在微流体系统内移动样品（例如流体）的系统和方法。 该系统包括形成流体流过的流体网络的衬底（130,132）和与衬底（130,132）成一体的多个气体致动器（168,170,172）。 一个这样的致动器（168）在第一位置处联接到网络，以提供气体压力以移动网络内的微流体样本。 另一气体致动器（170）在第二位置处联接到网络，用于提供气体压力以进一步移动网络内微流体样品的至少一部分。 阀（915,216）联接到微流体网络，使得当阀关闭时，阀将基本上将第二气体致动器与第一气体致动器隔离。