公开(公告)号：WO2008061165A3

公开(公告)日：2008-10-30

申请号：PCT/US2007084730

申请日：2007-11-14

Applicant: HANDYLAB INC ,  HANDIQUE KALYAN ,  PARUNAK GENE ,  HALEY CECELIA

Inventor： HANDIQUE KALYAN ,  PARUNAK GENE ,  HALEY CECELIA

IPC: B01L3/00 ,  F16K99/00

CPC classification number: B01L3/502738 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502723 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2200/142 ,  B01L2300/0803 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/0677 ,  B01L2400/0694 ,  C12Q1/6806 ,  C12Q1/686 ,  F16K99/0001 ,  F16K2099/008 ,  F16K2099/0084

Abstract: The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.

Abstract translation: 本发明技术提供了一种微流体基底，该微流体基底被配置为并行地对多个含多核苷酸的样品进行PCR。 基底可以是微流体盒中的单层基底。 还提供了制造包含这种基底的微流体盒的方法。 还进一步公开了适用于隔离微流体衬底中的PCR室的微流体阀以及制造这种阀的方法。

公开(公告)号：WO2005108620A3

公开(公告)日：2006-04-13

申请号：PCT/US2005015345

申请日：2005-05-03

Applicant: HANDYLAB INC ,  WU BETTY ,  ALTHAUS JOHN S ,  BRAHMASANDRA SUNDARESH N ,  HANDIQUE KALYAN ,  PHADKE NIKHIL

Inventor： WU BETTY ,  ALTHAUS JOHN S ,  BRAHMASANDRA SUNDARESH N ,  HANDIQUE KALYAN ,  PHADKE NIKHIL

IPC: B01F5/06 ,  B01F13/00 ,  B01L3/00 ,  B01L3/14 ,  B01L7/00 ,  C12N15/10 ,  C12Q1/68 ,  F16K99/00 ,  C12M1/34 ,  C12P19/34

CPC classification number: C12Q1/686 ,  B01F5/0646 ,  B01F5/0647 ,  B01F13/0059 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/5082 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0442 ,  B01L2400/0677 ,  B01L2400/0683 ,  B01L2400/0694 ,  C12N15/1006 ,  C12Q1/6806 ,  F16K99/0001 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/0036 ,  F16K99/0044 ,  F16K2099/0084 ,  C12Q2565/629

Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.

Abstract translation: 公开了用于处理多核苷酸（例如DNA）的方法和系统。 处理区域包括用在第一组条件（例如温度和pH）下重新获得多核苷酸的配体修饰的一个或多个表面（例如，颗粒表面），并在第二组条件（例如较高的温度和/ 或更多的碱性pH）。 处理区域可用于例如浓缩样品的多核苷酸和/或来自多核苷酸的分离的扩增反应抑制剂。 公开了具有处理区域的微流体装置。

公开(公告)号：WO2008060604A3

公开(公告)日：2009-02-19

申请号：PCT/US2007024022

申请日：2007-11-14

Applicant: HANDYLAB INC ,  HANDIQUE KALYAN ,  BRAHMASANDRA SUNDARESH N ,  GANESAN KARTHIK ,  WILLIAMS JEFF

Inventor： HANDIQUE KALYAN ,  BRAHMASANDRA SUNDARESH N ,  GANESAN KARTHIK ,  WILLIAMS JEFF

IPC: B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  G01N21/00

CPC classification number: B01L7/52 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502738 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2300/021 ,  B01L2300/022 ,  B01L2300/045 ,  B01L2300/046 ,  B01L2300/0861 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2300/1861 ,  C12Q1/686 ,  G01N21/6428 ,  G01N2021/6419 ,  Y10S435/808 ,  C12Q2565/629

Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.

Abstract translation: 本技术提供了用于检测样品中特别是生物样品中多核苷酸的装置。 该技术更特别涉及在微流体通道内对感兴趣的核苷酸进行PCR并检测这些核苷酸的微流体系统。 该装置包括被配置为接受多个样品并且可以分别对每个样品或一组或多个样品同时进行PCR的微流控盒。

公开(公告)号：WO2006119280A3

公开(公告)日：2007-02-15

申请号：PCT/US2006016814

申请日：2006-05-03

Applicant: HANDYLAB INC ,  PHADKE NIKHIL ,  MANENTE MICHELLE B ,  HANDIQUE KALYAN

Inventor： PHADKE NIKHIL ,  MANENTE MICHELLE B ,  HANDIQUE KALYAN

IPC: C12Q1/68

CPC classification number: F26B5/065 ,  C12Q1/6806 ,  F26B5/06 ,  C12Q2527/125

Abstract: Lyophilized pellets, suitable for use in a microfluidic device, and a method for preparing the same are described. The lyophilized pellets contain various biological reagents, or microparticles, and a cryoprotectant. The lyophilized pellets have a high degree of sphericity and are in the size range 0.5 to 35 µL. The pellets are prepared by dispensing drops of reagent solution on to a cryogenically cooled plate, followed by subjecting to a vacuum.

Abstract translation: 描述了适用于微流体装置的冻干颗粒及其制备方法。 冻干颗粒含有各种生物试剂或微粒，以及冷冻保护剂。 冻干颗粒具有高度的球形度，其尺寸范围为0.5至35μL。 通过将试剂溶液滴到低温冷却板上，然后进行真空来制备颗粒。

公开(公告)号：WO02078845A9

公开(公告)日：2002-12-12

申请号：PCT/US0209439

申请日：2002-03-27

Applicant: HANDYLAB INC

Inventor： HANDIQUE KALYAN ,  GANESAN KARTHIK ,  BRAHMASANDRA SUNDARESH N

IPC: G01N1/00 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  B81B1/00 ,  G01N1/28 ,  G01N37/00 ,  B01D57/02 ,  B01D59/42 ,  B01D59/50 ,  B01D61/42 ,  B01D61/58 ,  B01L3/02 ,  C02F1/40 ,  C02F11/00 ,  G01N1/10 ,  G01N27/26 ,  G01N27/27 ,  G01N27/403 ,  G01N27/453 ,  G01N31/00

CPC classification number: B01L7/52 ,  B01F13/0071 ,  B01F13/0079 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00835 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00862 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00936 ,  B01J2219/00943 ,  B01J2219/00945 ,  B01J2219/00961 ,  B01J2219/0097 ,  B01J2219/00986 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2300/0816 ,  B01L2400/0442 ,  B01L2400/0493 ,  B01L2400/0677 ,  G01N1/4077 ,  G01N35/1083 ,  Y10T436/12 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The present invention provides control methods, control systems, and control software for microfluidic devices that operate by moving discrete micro-droplets through a sequence of determined configurations. Such microfluidic devices are preferably constructed in a hierarchical and modular fashion which is reflected n the preferred structure of the provided methods and systems. In particular, the methods are structured into low -level device component control functions, middle-level actuator, control functions, and high-level micro-droplet control functions. Advantageously, a microfluidic device may thereby be instructed to perform an intended reaction or analysis by invoking micro-droplet control function that perform intuitive tasks like measuring (reaction/detectiong1), mixing (mixing 1), heating (heater1, heater2), and so forth. The systems are preferably programmable and capable of accommodating microfluidic devices controlled by low voltages and constructed in standardized configurations. Advantageously, a single control system can thereby control numerous different reactions in numerous different microfluidic devices simply by loading different easily understood micro-droplet programs.

Abstract translation: 本发明提供了用于微流体装置的控制方法，控制系统和控制软件，所述微流体装置通过将离散的微小液滴移动通过一系列确定的配置来操作。 这种微流体装置优选以分层和模块化的方式构建，其反映在所提供的方法和系统的优选结构上。 具体而言，这些方法被构造成低级设备组件控制功能，中级执行器，控制功能和高级微液滴控制功能。 有利的是，可以通过调用微滴控制功能来指示微流体装置执行预期的反应或分析，所述微滴控制功能执行直观的任务，如测量（反应/检测g1），混合（混合1），加热（加热器1，加热器2）等等 向前。 该系统优选是可编程的并且能够适应由低电压控制并且以标准化配置构建的微流体装置。 有利地，单个控制系统可以由此简单地通过加载不同的容易理解的微滴程序来控制许多不同微流体装置中的许多不同的反应。

公开(公告)号：WO2009054870A3

公开(公告)日：2009-08-20

申请号：PCT/US2008008640

申请日：2008-07-14

Applicant: HANDYLAB INC ,  WILLIAMS JEFF ,  WILSON KERRY ,  HANDIQUE KALYAN

Inventor： WILLIAMS JEFF ,  WILSON KERRY ,  HANDIQUE KALYAN

IPC: C12M1/00 ,  C12M3/00 ,  C12Q1/68

CPC classification number: C12Q1/6806 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502738 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/045 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/0442 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0611 ,  B01L2400/0677 ,  B01L2400/0683 ,  C12Q1/686 ,  F16K99/0001 ,  F16K99/003 ,  F16K99/0032 ,  F16K99/0044 ,  F16K99/0061 ,  F16K2099/0084 ,  G01N21/6428 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2035/00881

Abstract: The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.

Abstract translation: 本文描述的技术通常涉及用于从多个样品，特别是来自生物样品中提取多核苷酸的系统，另外还涉及随后扩增和检测提取的多核苷酸的系统。 该技术更特别涉及在微流体通道内的多个目的核苷酸样品上进行PCR并检测这些核苷酸的微流体系统。

公开(公告)号：WO2004094986A3

公开(公告)日：2005-02-17

申请号：PCT/US2004011900

申请日：2004-04-16

Applicant: HANDYLAB INC ,  ALTHAUS JOHN S ,  KYONGHOON LEE ,  NAMASIVAYAM VIJAY ,  BRAHMASANDRA SUNDARESH N ,  HANDIQUE KALYAN

Inventor： ALTHAUS JOHN S ,  KYONGHOON LEE ,  NAMASIVAYAM VIJAY ,  BRAHMASANDRA SUNDARESH N ,  HANDIQUE KALYAN

IPC: C12Q1/68 ,  G01N33/543 ,  G01N27/327

CPC classification number: G01N33/54373 ,  C12Q1/6825 ,  G01N33/5438 ,  Y10T436/143333 ,  C12Q2565/607 ,  C12Q2563/173

Abstract: The present invention relates to an electrochemical method for detecting a target polynucleotide. An electrode comprising an electrode surface is provided. The electrode surface includes at least one probe molecule reverisbly immobilized with respect to the electrode surface. A first electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The electrode surface is contacted with a liquid comprising the target polynucleotide. Upon the contacting step, at least some of the probe molecule immobilized with respect to the electrode surface dissociates therefrom. A second electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The presence of the target polynucleotide is determined at least partially on the basis of the first and second electrochemical signals.

Abstract translation: 本发明涉及一种用于检测靶多核苷酸的电化学方法。 提供了包括电极表面的电极。 电极表面包括相对于电极表面反复固定的至少一个探针分子。 获得指示相对于电极表面固定的探针分子的量的第一电化学信号。 电极表面与包含靶多核苷酸的液体接触。 在接触步骤中，相对于电极表面固定的至少一些探针分子与其分离。 获得指示相对于电极表面固定的探针分子的量的第二电化学信号。 至少部分地基于第一和第二电化学信号确定靶多核苷酸的存在。

公开(公告)号：WO2007044917A3

公开(公告)日：2007-07-26

申请号：PCT/US2006040171

申请日：2006-10-11

Applicant: HANDYLAB INC ,  HANDIQUE KALYAN ,  WILLIAMS JEFF ,  BRAHMASANDRA SUNDARESH N ,  PHADKE NIKHIL ,  WU BETTY

Inventor： HANDIQUE KALYAN ,  WILLIAMS JEFF ,  BRAHMASANDRA SUNDARESH N ,  PHADKE NIKHIL ,  WU BETTY

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: B01L3/502715 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502738 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/0867 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/0487 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/0034 ,  F16K99/0044 ,  F16K99/0061 ,  F16K2099/0084 ,  G01N1/34 ,  G01N2035/00158

Abstract: Methods and systems for preparing polynucleotide samples are disclosed. The invention includes a microfluidic system for converting a sample containing one or more polynucleotides into a form suitable for analyzing the polynucleotides, comprising: a cartridge receiving element, an insertable and removable cartridge, a heating element configured to heat one or more regions of the cartridge, and control circuitry, wherein the insertable cartridge comprises: a microfluidic component that is configured to accept the sample and one or more reagents, and to react the sample and the reagents, in order to produce a prepared sample suitable for analyzing the one or more polynucleotides. The invention further comprises a multi-sample cartridge for converting a number of samples, each containing one or more polynucleotides, into respective forms suitable for analyzing the polynucleotides, comprising: at least a first microfluidic component and a second microfluidic component.

Abstract translation: 公开了制备多核苷酸样品的方法和系统。 本发明包括用于将含有一种或多种多核苷酸的样品转化成适于分析多核苷酸的形式的微流体系统，包括：盒接收元件，可插入和可移除的盒，加热元件，其配置成加热盒的一个或多个区域 以及控制电路，其中所述可插入筒包括：微流体组分，其被配置为接受所述样品和一种或多种试剂，并使所述样品和所述试剂反应，以便产生适于分析所述一种或多种 多核苷酸。 本发明还包括用于将多个样品（各自含有一个或多个多核苷酸）转化成适于分析多核苷酸的各自形式的多样品盒，包括：至少第一微流体组分和第二微流体组分。

公开(公告)号：WO03012406A9

公开(公告)日：2003-03-20

申请号：PCT/US0209440

申请日：2002-03-27

Applicant: HANDYLAB INC

Inventor： PARUNAK GENE ,  HANDIQUE KALYAN ,  WU BETTY ,  GANESAN KARTHIK

IPC: G11C16/06 ,  B01L3/00 ,  B81B1/00 ,  F04B19/00 ,  F04B19/24 ,  F04F1/06 ,  G01N21/29 ,  B01D57/02 ,  C02F1/40 ,  C12M1/34 ,  C12M1/36 ,  C12N13/00 ,  C12Q1/68

CPC classification number: B01L3/502738 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2400/0442

Abstract: The present invention relates to a system and method for moving samples, such as fluids, within a microfluidic system using a plurality of gas actuators (168, 170, 172) for applying pressure at different locations within the microfluidic. The system includes a substrate (130, 132) which forms a fluid network through which fluid flows, and a plurality of gas actuators (168, 170, 172) integral with the substrate (130, 132). One such actuator (168) is coupled to the network at a first location for providing gas pressure to move a microfluidic sample within the network. Another gas actuator (170) is coupled to the network at a second location for providing gas pressure to further move at least a portion of the microfluidic sample within the network. A valve (915, 216) is coupled to the microfluidic network so that, when the valve is closed, it substantially isolates the second gas actuator from the first gas actuator.

Abstract translation: 本发明涉及一种使用多个用于在微流体内的不同位置施加压力的气体致动器（168,170,172）在微流体系统内移动样品（例如流体）的系统和方法。 该系统包括形成流体流过的流体网络的衬底（130,132）和与衬底（130,132）成一体的多个气体致动器（168,170,172）。 一个这样的致动器（168）在第一位置处联接到网络，以提供气体压力以移动网络内的微流体样本。 另一气体致动器（170）在第二位置处联接到网络，用于提供气体压力以进一步移动网络内微流体样品的至少一部分。 阀（915,216）联接到微流体网络，使得当阀关闭时，阀将基本上将第二气体致动器与第一气体致动器隔离。

公开(公告)号：WO2007112114A2

公开(公告)日：2007-10-04

申请号：PCT/US2007007513

申请日：2007-03-26

Applicant: HANDYLAB INC ,  HANDIQUE KALYAN ,  BRAHMASANDRA SUNDARESH N ,  GANESAN KARTHIK ,  WU BETTY ,  PHADKE NIKHIL ,  PARUNAK GENE ,  WILLIAMS JEFF

Inventor： HANDIQUE KALYAN ,  BRAHMASANDRA SUNDARESH N ,  GANESAN KARTHIK ,  WU BETTY ,  PHADKE NIKHIL ,  PARUNAK GENE ,  WILLIAMS JEFF

IPC: C12M3/00

CPC classification number: B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/06 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/18 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/0442 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0611 ,  B01L2400/0677 ,  B01L2400/0683 ,  F16K99/0001 ,  F16K99/003 ,  F16K99/0032 ,  F16K99/0044 ,  F16K99/0061 ,  F16K2099/0084 ,  G01N2035/00881

Abstract: This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.

Abstract translation: 该专利申请描述了用于处理含有多核苷酸的样品并用于在其上提供诊断结果的集成设备。 该装置被配置为接收包含用于处理样品的试剂和网络的微流控盒。 还描述了使用该装置的方法。