公开(公告)号：WO2004017062A8

公开(公告)日：2004-10-21

申请号：PCT/CN0200882

申请日：2002-12-11

Applicant: UNIV TSINGHUA ,  CAPITAL BIOCHIP CO LTD ,  LI GANG ,  XING WANLI ,  WANG DONG ,  GAO HUAFANG ,  GUO MIN ,  CHENG JING

Inventor： LI GANG ,  XING WANLI ,  WANG DONG ,  GAO HUAFANG ,  GUO MIN ,  CHENG JING

IPC: A01H1/02 ,  B01F11/02 ,  B01F13/00 ,  B06B1/20 ,  C07H21/00 ,  C12M1/42 ,  C12N13/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N29/00

CPC classification number: B01F13/0059 ,  B01F11/0258 ,  B01J2219/00479 ,  C12Q1/6832

Abstract: The present method and device is for accelerating molecular hybridization by ultrasonic wave. A prepared hybridization reaction system is placed in the path of ultrasonic wave generated by an ultrasonic generator. High frequency vibration and irradiating pressure of ultrasonic wave result in effective agitation and flow of liquid medium. The flow of liquid medium then carries reaction molecular to move so as to speed up the hybridization reaction. Advantages of the invention include increasing the intensity of hybridization signal significantly so that a complete reaction can be obtained and time can be saved; and improving consistency of hybridization reaction at different locations on the surface of solid medium, uniformity of hybridization and idiosyncrasy of hybridization reaction. Main parts of the device are an ultrasonic resonance element and the corresponding resonance electrocircuit. The device has simple structure with low cost and can be operated easily and automatically. It can be applied to various hybridization reaction systems, such as Southern, Northern and genetic chips.

Abstract translation: 本发明的方法和装置用于加速超声波的分子杂交。 将制备的杂交反应体系置于由超声波发生器产生的超声波的路径中。 超声波的高频振动和辐射压力导致液体介质的有效搅动和流动。 液体介质的流动使得反应分子运动以加速杂交反应。 本发明的优点包括显着提高杂交信号的强度，从而可以获得完整的反应并节省时间; 提高固体培养基表面不同位置的杂交反应的一致性，杂交的一致性和杂交反应的特异性。 该装置的主要部件是超声波共振元件和相应的共振电路。 该装置结构简单，成本低，操作方便，自动化。 它可以应用于各种杂交反应体系，如Southern，Northern和遗传芯片。

公开(公告)号：WO2004079002A8

公开(公告)日：2005-12-01

申请号：PCT/CN0300328

申请日：2003-05-06

Applicant: UNIV TSINGHUA ,  CAPITAL BIOCHIP CO LTD ,  TAO SHENGE ,  CHENG JING ,  MAX XUMEI ,  ZHOU YUXIANG

Inventor： TAO SHENGE ,  CHENG JING ,  MAX XUMEI ,  ZHANG QIONG ,  ZHOU YUXIANG

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14

CPC classification number: B01L3/508 ,  B01J2219/00385 ,  B01J2219/00527 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00722 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/163 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  C12Q2547/101 ,  C12Q2531/131

Abstract: This invention relates generally to the field of nucleic acid detection. In particular, the invention provides a lab-on-chip system for analyzing a nucleic acid, which system comprises, inter alia, controllably closed space, and a target nucleic acid can be prepared and/or amplified, and hybridized to a nucleic acid probe, and the hybridization signal can be acquired if desirable, in the controllably closed space without any material exchange between the controllably closed space and the outside environment. Methods for analyzing a nucleic acid using the lab-on-chip system is also provided.

Abstract translation: 本发明一般涉及核酸检测领域。 特别地，本发明提供了用于分析核酸的片上实验室系统，该系统尤其包括可控制的封闭空间，并且靶核酸可以制备和/或扩增，并与核酸探针杂交 ，并且如果需要，可以在可控制的封闭空间中获取杂交信号，而在可控制的封闭空间和外部环境之间没有任何物质交换。 还提供了使用片上实验室系统分析核酸的方法。

公开(公告)号：WO0202794A3

公开(公告)日：2002-08-15

申请号：PCT/IB0101530

申请日：2001-07-02

Applicant: CAPITAL BIOCHIP CO LTD ,  UNIV TSINGHUA

Inventor： SHAO WEI ,  XU JUNQUAN ,  XING WAN-LI ,  CHENG JING

IPC: G01N33/53 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  B81B1/00 ,  B81B7/04 ,  C12M1/00 ,  C12N15/09 ,  C40B40/06 ,  C40B60/14 ,  G01N33/543 ,  G01N35/00 ,  G01N37/00 ,  C12Q1/68 ,  C12M1/38

CPC classification number: B01L3/50851 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00351 ,  B01J2219/00495 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/0074 ,  B01J2219/00743 ,  B01L3/502 ,  B01L7/00 ,  B01L9/52 ,  B81B7/04 ,  C40B40/06 ,  C40B60/14 ,  G01N33/54373 ,  G01N35/00029 ,  G01N2035/00158 ,  Y10S977/924

Abstract: This invention relates generally to the field of microarray technology. In particular, the invention provides an integrated macroarray device, which device comprises a substrate comprising a plurality of distinct microlocations and a plurality of microarray chips, wherein the number of said microlocations equals to or is more then the number of said microarray chip. In preferred embodiments, the devices also comprises a temperature controller at some or all of the microlocations. The use of the integrated microarray devices for detecting interactions among various moieties in various fields, such as clinical diagnostics, drug discovery, environmental monitoring and forensic analysis, etc., are further provided.

Abstract translation: 本发明一般涉及微阵列技术领域。 特别地，本发明提供了一种集成的宏阵列装置，该装置包括含有多个不同的微位点和多个微阵列芯片的基底，其中所述微位点的数量等于或大于所述微阵列芯片的数量。 在优选的实施方案中，所述装置在一些或全部微位置处还包含温度控制器。 进一步提供了使用集成微阵列装置来检测各个领域各种部分之间的相互作用，例如临床诊断，药物发现，环境监测和法医分析等。