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公开(公告)号：WO2010027484A9
公开(公告)日：2010-03-11
申请号：PCT/US2009/004993
申请日：2009-09-04
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. , PATEL, Pranav , KORLACH, Jonas , BIBILLO, Arkadiusz , BJORNSON, Keith , HANES, Jeremiah
Inventor： PATEL, Pranav , KORLACH, Jonas , BIBILLO, Arkadiusz , BJORNSON, Keith , HANES, Jeremiah
Abstract: Provided are methods for enhanced sequencing of nucleic acid templates. Also provided are reaction conditions that increase branching fractions during polymerization reactions. Also provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are higher than the branching fractions of the polymerases from which they were derived. Provided are compositions comprising modified recombinant polymerases that exhibit delayed translocation relative to the polymerases from which they were derived. Also provided are compositions comprising modified recombinant polymerases that exhibit increased nucleotide or nucleotide analog residence time at an active site of the polymerase. Provided are methods for generating polymerases with the aforementioned phenotypes and methods of using such polymerases to sequence a DNA template or make a DNA. Also provided are methods and nucleic acid sequencing systems for determining which labeled nucleotide is incorporated at a site during a template-dependent polymerization reaction.
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公开(公告)号：WO2012065043A2
公开(公告)日：2012-05-18
申请号：PCT/US2011/060338
申请日：2011-11-11
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. , FLUSBERG, Benjamin , KORLACH, Jonas , WEGENER, Jeffrey , CLARK, Tyson, A. , VILFAN, Igor , KISLYUK, Andrey , TURNER, Stephen , SORENSON, Jon , TRAVERS, Kevin , HEINER, Cheryl , TOMANEY, Austin, B. , MARKS, Patrick , LEE, Jessica , JIA, Lei , WEBSTER, Dale , LYLE, John , HANES, Jeremiah , PUGLISI, Joseph
Inventor： FLUSBERG, Benjamin , KORLACH, Jonas , WEGENER, Jeffrey , CLARK, Tyson, A. , VILFAN, Igor , KISLYUK, Andrey , TURNER, Stephen , SORENSON, Jon , TRAVERS, Kevin , HEINER, Cheryl , TOMANEY, Austin, B. , MARKS, Patrick , LEE, Jessica , JIA, Lei , WEBSTER, Dale , LYLE, John , HANES, Jeremiah , PUGLISI, Joseph
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q1/6874 , C12Q1/689 , C12Q2600/154 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
Abstract translation: 提供了用于表征修饰的核酸的方法，组合物和系统。 在某些优选的实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰包括化学修饰的碱基，酶修饰的碱基，无碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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3.发明申请
公开(公告)号：WO2012009206A2
公开(公告)日：2012-01-19
申请号：PCT/US2011043227
申请日：2011-07-07
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , FEDOROV ANDREI , LYLE JOHN , BJORNSON KEITH , HANES JEREMIAH
Inventor： FEDOROV ANDREI , LYLE JOHN , BJORNSON KEITH , HANES JEREMIAH
CPC classification number: C12Q1/6869 , C12Q2527/125 , C12Q2563/137
Abstract: Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having monovalent cations that control the median pulse width for incorporated nucleotides are disclosed. The levels of alkali metals such as lithium, sodium, potassium, rubidium, and cesium in the polymerization are used to control pulse width while allowing other sequencing parameters to remain within a desirable range.
Abstract translation: 公开了用于单分子核苷酸测序的组合物，试剂盒，方法和系统，其包括产生具有控制掺入的核苷酸的中值脉冲宽度的具有一价阳离子的聚合酶反应。 聚合中的锂，钠，钾，铷和铯等碱金属的含量被用于控制脉冲宽度，同时允许其它测序参数保持在期望的范围内。
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公开(公告)号：WO2010068289A2
公开(公告)日：2010-06-17
申请号：PCT/US2009/006517
申请日：2009-12-10
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. , FLUSBERG, Benjamin , TURNER, Stephen , LEE, Jessica , JIA, Lei , KORLACH, Jonas , SORENSON, Jon , WEBSTER, Dale , LYLE, John , TRAVERS, Kevin , HANES, Jeremiah
Inventor： FLUSBERG, Benjamin , TURNER, Stephen , LEE, Jessica , JIA, Lei , KORLACH, Jonas , SORENSON, Jon , WEBSTER, Dale , LYLE, John , TRAVERS, Kevin , HANES, Jeremiah
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
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公开(公告)号：WO2012065043A9
公开(公告)日：2012-12-20
申请号：PCT/US2011060338
申请日：2011-11-11
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , FLUSBERG BENJAMIN , KORLACH JONAS , WEGENER JEFFREY , CLARK TYSON A , VILFAN IGOR , KISLYUK ANDREY , TURNER STEPHEN , SORENSON JON , TRAVERS KEVIN , HEINER CHERYL , TOMANEY AUSTIN B , MARKS PATRICK , LEE JESSICA , JIA LEI , WEBSTER DALE , LYLE JOHN , HANES JEREMIAH , PUGLISI JOSEPH
Inventor： FLUSBERG BENJAMIN , KORLACH JONAS , WEGENER JEFFREY , CLARK TYSON A , VILFAN IGOR , KISLYUK ANDREY , TURNER STEPHEN , SORENSON JON , TRAVERS KEVIN , HEINER CHERYL , TOMANEY AUSTIN B , MARKS PATRICK , LEE JESSICA , JIA LEI , WEBSTER DALE , LYLE JOHN , HANES JEREMIAH , PUGLISI JOSEPH
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q1/6874 , C12Q1/689 , C12Q2600/154 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
Abstract translation: 提供了用于表征修饰的核酸的方法，组合物和系统。 在某些优选的实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰包括化学修饰的碱基，酶修饰的碱基，无碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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Patent Agency Ranking
公开(公告)号：WO2012065043A3
公开(公告)日：2012-08-16
申请号：PCT/US2011060338
申请日：2011-11-11
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , FLUSBERG BENJAMIN , KORLACH JONAS , WEGENER JEFFREY , CLARK TYSON A , VILFAN IGOR , KISLYUK ANDREY , TURNER STEPHEN , SORENSON JON , TRAVERS KEVIN , HEINER CHERYL , TOMANEY AUSTIN B , MARKS PATRICK , LEE JESSICA , JIA LEI , WEBSTER DALE , LYLE JOHN , HANES JEREMIAH , PUGLISI JOSEPH
Inventor： FLUSBERG BENJAMIN , KORLACH JONAS , WEGENER JEFFREY , CLARK TYSON A , VILFAN IGOR , KISLYUK ANDREY , TURNER STEPHEN , SORENSON JON , TRAVERS KEVIN , HEINER CHERYL , TOMANEY AUSTIN B , MARKS PATRICK , LEE JESSICA , JIA LEI , WEBSTER DALE , LYLE JOHN , HANES JEREMIAH , PUGLISI JOSEPH
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q1/6874 , C12Q1/689 , C12Q2600/154 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
Abstract translation: 提供了方法，组合物和系统用于表征修饰的核酸。 在某些优选实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基，酶修饰的碱基，脱碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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公开(公告)号：WO2012009206A3
公开(公告)日：2012-01-19
申请号：PCT/US2011/043227
申请日：2011-07-07
Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC. , FEDOROV, Andrei , LYLE, John , BJORNSON, Keith , HANES, Jeremiah
Inventor： FEDOROV, Andrei , LYLE, John , BJORNSON, Keith , HANES, Jeremiah
Abstract: Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having monovalent cations that control the median pulse width for incorporated nucleotides are disclosed. The levels of alkali metals such as lithium, sodium, potassium, rubidium, and cesium in the polymerization are used to control pulse width while allowing other sequencing parameters to remain within a desirable range.
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8.发明申请
公开(公告)号：WO2010027484A2
公开(公告)日：2010-03-11
申请号：PCT/US2009004993
申请日：2009-09-04
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , PATEL PRANAV , KORLACH JONAS , BIBILLO ARKADIUSZ , BJORNSON KEITH , HANES JEREMIAH
Inventor： PATEL PRANAV , KORLACH JONAS , BIBILLO ARKADIUSZ , BJORNSON KEITH , HANES JEREMIAH
CPC classification number: C12Q1/6869 , C12N9/1252 , C12Q2527/113 , C12Q2521/101 , C12Q2527/137 , C12Q2537/161 , C12Q2525/101 , C12Q2563/107
Abstract: Provided are methods for enhanced sequencing of nucleic acid templates. Also provided are reaction conditions that increase branching fractions during polymerization reactions. Also provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are higher than the branching fractions of the polymerases from which they were derived. Provided are compositions comprising modified recombinant polymerases that exhibit delayed translocation relative to the polymerases from which they were derived. Also provided are compositions comprising modified recombinant polymerases that exhibit increased nucleotide or nucleotide analog residence time at an active site of the polymerase. Provided are methods for generating polymerases with the aforementioned phenotypes and methods of using such polymerases to sequence a DNA template or make a DNA. Also provided are methods and nucleic acid sequencing systems for determining which labeled nucleotide is incorporated at a site during a template-dependent polymerization reaction.
Abstract translation: 提供了用于增强核酸模板测序的方法。 还提供了在聚合反应期间增加支化馏分的反应条件。 还提供了包含改性重组聚合酶的组合物，其表现出高于其衍生的聚合酶的支化部分的支化部分。 提供了包含修饰的重组聚合酶的组合物，其相对于衍生自其的聚合酶表现出延迟的移位。 还提供了包含修饰的重组聚合酶的组合物，其在聚合酶的活性位点表现出增加的核苷酸或核苷酸类似物停留时间。 提供了使用上述表型产生聚合酶的方法和使用这种聚合酶来顺序DNA模板或制备DNA的方法。 还提供了用于在模板依赖性聚合反应期间确定哪个标记的核苷酸被掺入位点的方法和核酸测序系统。
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公开(公告)号：WO2010068289A3
公开(公告)日：2010-10-14
申请号：PCT/US2009006517
申请日：2009-12-10
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , FLUSBERG BENJAMIN , TURNER STEPHEN , LEE JESSICA , JIA LEI , KORLACH JONAS , SORENSON JON , WEBSTER DALE , LYLE JOHN , TRAVERS KEVIN , HANES JEREMIAH
Inventor： FLUSBERG BENJAMIN , TURNER STEPHEN , LEE JESSICA , JIA LEI , KORLACH JONAS , SORENSON JON , WEBSTER DALE , LYLE JOHN , TRAVERS KEVIN , HANES JEREMIAH
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
Abstract translation: 提供了方法，组合物和系统用于表征修饰的核酸。 在某些优选实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基，酶修饰的碱基，脱碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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10.发明申请ENGINEERING POLYMERASES AND REACTION CONDITIONS FOR MODIFIED INCORPORATION PROPERTIES 审中-公开工程聚合物与改性掺入特性的反应条件
公开(公告)号：WO2010027484A3
公开(公告)日：2010-06-24
申请号：PCT/US2009004993
申请日：2009-09-04
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , PATEL PRANAV , KORLACH JONAS , BIBILLO ARKADIUSZ , BJORNSON KEITH , HANES JEREMIAH
Inventor： PATEL PRANAV , KORLACH JONAS , BIBILLO ARKADIUSZ , BJORNSON KEITH , HANES JEREMIAH
CPC classification number: C12Q1/6869 , C12N9/1252 , C12Q2527/113 , C12Q2521/101 , C12Q2527/137 , C12Q2537/161 , C12Q2525/101 , C12Q2563/107
Abstract: Provided are methods for enhanced sequencing of nucleic acid templates. Also provided are reaction conditions that increase branching fractions during polymerization reactions. Also provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are higher than the branching fractions of the polymerases from which they were derived. Provided are compositions comprising modified recombinant polymerases that exhibit delayed translocation relative to the polymerases from which they were derived. Also provided are compositions comprising modified recombinant polymerases that exhibit increased nucleotide or nucleotide analog residence time at an active site of the polymerase. Provided are methods for generating polymerases with the aforementioned phenotypes and methods of using such polymerases to sequence a DNA template or make a DNA. Also provided are methods and nucleic acid sequencing systems for determining which labeled nucleotide is incorporated at a site during a template-dependent polymerization reaction.
Abstract translation: 提供了用于增强核酸模板测序的方法。 还提供了在聚合反应期间增加支化部分的反应条件。 还提供了包含修饰的重组聚合酶的组合物，其表现出比衍生它们的聚合酶的支化部分更高的支化部分。 提供了包含相对于衍生它们的聚合酶显示延迟易位的修饰的重组聚合酶的组合物。 还提供了包含修饰的重组聚合酶的组合物，所述修饰的重组聚合酶在聚合酶的活性位点显示增加的核苷酸或核苷酸类似物停留时间 提供了产生具有上述表型的聚合酶的方法和使用这些聚合酶测序DNA模板或制备DNA的方法。 还提供了用于确定哪些标记的核苷酸在模板依赖性聚合反应期间在位点处掺入的方法和核酸测序系统。
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