公开(公告)号：WO2016126871A2

公开(公告)日：2016-08-11

申请号：PCT/US2016/016444

申请日：2016-02-03

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： ABATE, Adam R. ,  HALIBURTON, John ,  LAN, Freeman ,  SCIAMBI, Adam R.

IPC: C12Q1/68 ,  C40B30/06

CPC classification number: C12Q1/6804 ,  B01F5/0655 ,  B01F13/0071 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2300/021 ,  B01L2300/0645 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/043 ,  C12N15/1096 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q2535/122 ,  C12Q2563/159 ,  C12Q2563/179

Abstract: Microfluidic methods for barcoding nucleic acid target molecules to be analyzed, e.g., via nucleic acid sequencing techniques, are provided. Also provided are microfluidic, droplet-based methods of preparing nucleic acid barcodes for use in various barcoding applications. The methods described herein facilitate high-throughput sequencing of nucleic acid target molecules as well as single cell and single virus genomic, transcriptomic, and/or proteomic analysis/profiling. Systems and devices for practicing the subject methods are also provided.

Abstract translation: 提供了用于条形码化待分析的核酸靶分子的微流控方法，例如通过核酸测序技术。 还提供了用于各种条形码应用的制备核酸条形码的微流体，基于微滴的方法。 本文所述的方法有助于核酸靶分子的高通量测序以及单细胞和单一病毒基因组，转录组学和/或蛋白质组分析/分析。 还提供了用于练习主题方法的系统和设备。

公开(公告)号：WO2016065056A1

公开(公告)日：2016-04-28

申请号：PCT/US2015/056743

申请日：2015-10-21

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： ABATE, Adam R. ,  SCIAMBI, Adam R. ,  COLE, Russell ,  GARTNER, Zev Jordan

IPC: B01L3/00 ,  G01N33/50 ,  G01N35/10

CPC classification number: B01L3/0268 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2400/0427 ,  B01L2400/086 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/1034

Abstract: Methods for delivering discrete entities including, e.g., cells, media or reagents to substrates are provided. In certain aspects, the methods include manipulating and/or analyzing qualities of the entities or biological components thereof. In some embodiments, the methods may be used to create arrays of microenvironments and/or for two and three-dimensional printing of tissues or structures. Systems and devices for practicing the subject methods are also provided.

Abstract translation: 提供了将离散实体（例如，诸如细胞，培养基或试剂）输送到基底的方法。 在某些方面，所述方法包括操纵和/或分析实体或其生物组分的质量。 在一些实施方案中，所述方法可用于产生微环境阵列和/或用于组织或结构的二维和三维打印。 还提供了用于实践主题方法的系统和设备。

公开(公告)号：WO2014028378A2

公开(公告)日：2014-02-20

申请号：PCT/US2013/054517

申请日：2013-08-12

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： ABATE, Adam R. ,  EASTBURN, Dennis Jay ,  SCIAMBI, Adam R.

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01F3/0807 ,  B01F5/0652 ,  B01F13/0062 ,  B01F13/0076 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q2600/158 ,  C12Q2563/159 ,  C12Q2537/143 ,  C12Q2565/629

Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.

Abstract translation: 提供了用于检测生物样品中组分的方法。 在某些方面，所述方法可以用于检测和/或定量生物样品中的特定组分，例如肿瘤细胞（例如，循环肿瘤细胞）。 还提供了用于练习主题方法的系统和设备。

公开(公告)号：WO2015200717A3

公开(公告)日：2015-12-30

申请号：PCT/US2015/037822

申请日：2015-06-25

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： ABATE, Adam R. ,  EASTBURN, Dennis Jay ,  SCIAMBI, Adam R. ,  LIM, Shaun

IPC: B05B1/02

Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.