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    GENETIC SYSTEM FOR THE PRODUCTION OF LEUPEPTIN AND ITS USE FOR HETEROLOGOUS PROTEIN PRODUCTION
    5.
    发明申请
    GENETIC SYSTEM FOR THE PRODUCTION OF LEUPEPTIN AND ITS USE FOR HETEROLOGOUS PROTEIN PRODUCTION 审中-公开
    用于生产利培酮的遗传系统及其用于异位蛋白质生产的用途

    公开(公告)号：WO2016193954A3

    公开(公告)日：2017-01-26

    申请号：PCT/IB2016053295

    申请日：2016-06-05

    申请人： CENTRO DE INVESTIGACIÓN Y DE ESTUDIOS AVANZADOS DEL INST POLITÉCNICO NAC

    发明人： CRUZ MORALES PABLO ,  BARONA GÓMEZ FRANCISCO ,  RAMOS ABOITES HILDA ERÉNDIRA ,  MORALES ESCALANTE MARCO ANTONIO

    IPC分类号： C07K5/083 ,  C12N9/00 ,  C12N9/10 ,  C12N9/88 ,  C12N15/54 ,  C12N15/60

    CPC分类号： C12N15/52 ,  C07K5/0808 ,  C12N9/00 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/88 ,  C12P21/02

    摘要： The present invention describes the discovery of the genes that direct the biosynthetic pathway of leupeptin, a potent protease inhibitor of the type of small peptide aldehyde (SPA). The prediction of genes that direct the biosynthesis of leupeptin was performed by EvoMining, a bioinformatic pipe for genome data mining of natural products based on evolutionary parameters and was confirmed by molecular biology techniques such as insertional mutagenesis and the comparison of metabolomic profiles between wild strains (producers of leupeptin) and mutants (non producers of leupeptin). Biosynthetic genes of leupeptin were cloned and expressed heterologously in other expression systems of bacterial origin, thus achieving the production of leupeptin. It also describes the design of a cassette for heterologous production of leupeptin and its use for producing leupeptin and variants of it using synthetic biology, and to produce recombinant proteins.

    摘要翻译： 本发明描述了引导亮肽素生物合成途径的基因的发现，这是一种小肽醛（SPA）类型的强力蛋白酶抑制剂。 通过EvoMining（基于进化参数的天然产物基因组数据挖掘的生物信息学管道）进行指导亮色素抑制素生物合成的基因的预测，并通过分子生物学技术如插入突变和野生菌株间代谢组学比较 亮抑酶肽的生产者）和突变体（亮抑酶肽的非生产者）。 亮抑酶肽的生物合成基因在其他细菌来源的表达系统中被异源克隆并表达，从而实现亮抑酶肽的生产。 它还描述了用于异源生产亮抑酶肽的盒的设计及其用于使用合成生物学产生亮抑酶肽及其变体并用于产生重组蛋白的用途。

    SELF-ASSEMBLED ULTRASHORT PEPTIDES HYDROGELS FOR WOUND HEALING, SKIN CARE AND COSMETICS APPLICATIONS
    10.
    发明申请
    SELF-ASSEMBLED ULTRASHORT PEPTIDES HYDROGELS FOR WOUND HEALING, SKIN CARE AND COSMETICS APPLICATIONS 审中-公开
    自组装超声波胶囊用于治疗健康，皮肤护理和化妆品应用

    公开(公告)号：WO2014104981A1

    公开(公告)日：2014-07-03

    申请号：PCT/SG2013/000564

    申请日：2013-12-31

    申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    发明人： HAUSER, Charlotte ,  LOO, Yihua

    IPC分类号： C07K7/06 ,  C07K5/103 ,  C07K5/083 ,  A61K38/08 ,  A61K38/07 ,  A61K38/06 ,  A61K47/42

    CPC分类号： C07K5/06034 ,  A61K38/00 ,  C07K5/06026 ,  C07K5/0806 ,  C07K5/0808 ,  C07K5/1008 ,  C07K5/101 ,  C07K7/06

    摘要： The present invention provides an amphiphilic linear peptide and/or peptoid as well as a hydrogel that includes the amphiphilic linear peptide/peptoid. The amphiphilic linear peptide/peptoid is capable of self-assembling into three-dimensional macromolecular nanofibrous networks, which entrap water, and forming a hydrogel. These peptides/peptoids include short amphiphilic sequences with a hydrophobic portion of aliphatic amino acids and at least one acidic, neutral, or basic polar amino acid. The amphiphilic linear peptide/peptoid is build up of non repetitive aliphatic amino acids, which may be in the L- or D-form. A plurality of such peptides/peptoids assembles to supramolecular helical fibers and forms peptide hydrogels after assembly. A corresponding hydrogel is formed in aqueous solutions at physiological pH and is thus useful for inter alia cell culture, tissue engineering, tissue regeneration, wound healing and release of bioactive moieties (including cells, nucleic acids, anti-microbials, micro-/nanoparticles, cosmetic agents and small molecule therapeutics), as well as for providing mechanical support for damaged or missing tissues. Such hydrogels can also be formed in situ, wherein the gelation process occurs within the body following the injection of a peptide solution. Such hydrogels, which are rigid, biocompatible and entrap up to 99.9% of water, are also well suited for applications utilizing electronic devices.

    摘要翻译： 本发明提供两亲性线性肽和/或拟肽以及包含两亲性线性肽/拟肽的水凝胶。 两亲性线性肽/拟肽能够自组装成三维大分子纳米纤维网络，其捕获水并形成水凝胶。 这些肽/拟肽包括具有脂族氨基酸的疏水部分和至少一种酸性，中性或碱性极性氨基酸的短两亲序列。 两亲性线性肽/拟肽是由非重复脂肪族氨基酸组成的，它们可以是L-或D-形式。 多个这样的肽/拟肽组装成超分子螺旋纤维，并在组装后形成肽水凝胶。 相应的水凝胶在生理pH下在水溶液中形成，因此可用于特别是细胞培养，组织工程，组织再生，伤口愈合和释放生物活性部分（包括细胞，核酸，抗微生物，微/纳米颗粒， 化妆品和小分子治疗剂），以及为损坏或缺失的组织提供机械支持。 这种水凝胶也可以原位形成，其中在注射肽溶液之后，凝胶化过程发生在体内。 这种刚性，生物相容性和截留高达99.9％的水的水凝胶也非常适用于利用电子设备的应用。