公开(公告)号：WO2009042163A2

公开(公告)日：2009-04-02

申请号：PCT/US2008011087

申请日：2008-09-25

Applicant: SANGAMO BIOSCIENCES INC ,  DOYON YANNICK ,  URNOV FYODOR

Inventor： DOYON YANNICK ,  URNOV FYODOR

IPC: C12Q1/34 ,  G01N33/573

CPC classification number: C12Q1/6897 ,  C12N9/22 ,  C12Q1/34 ,  G01N33/573 ,  G01N2333/922

Abstract: Disclosed herein are methods and compositions for rapidly identifying and ranking nucleases for specific cleavage of a target sequence.

Abstract translation: 本文公开了用于快速鉴定和排列用于靶序列的特异性切割的核酸酶的方法和组合物。

公开(公告)号：WO2009042186A3

公开(公告)日：2009-05-22

申请号：PCT/US2008011136

申请日：2008-09-25

Applicant: SANGAMO BIOSCIENCES INC ,  UNIV CALIFORNIA ,  AMACHER SHARON ,  DOYON YANNICK ,  MCCAMMON JASMINE ,  URNOV FYODOR

Inventor： AMACHER SHARON ,  DOYON YANNICK ,  MCCAMMON JASMINE ,  URNOV FYODOR

IPC: C12N9/22 ,  A01K67/027 ,  C07K19/00 ,  C12N5/10 ,  C12N15/55 ,  C12N15/62

CPC classification number: A01K67/0276 ,  A01K2217/075 ,  A01K2227/40 ,  A01K2267/03 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/22 ,  C12N2800/80

Abstract: Disclosed herein are methods and compositions for genomic editing of one or more genes in zebrafish, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.

Abstract translation: 本文公开了使用包含锌指蛋白和切割结构域或切割半结构域的融合蛋白对斑马鱼中的一种或多种基因进行基因组编辑的方法和组合物。 还提供了编码所述融合蛋白的多核苷酸，以及包含所述多核苷酸和融合蛋白的细胞。

公开(公告)号：WO03027247A3

公开(公告)日：2003-05-22

申请号：PCT/US0230413

申请日：2002-09-24

Applicant: SANGAMO BIOSCIENCES INC ,  WOLFFE LIZ LF ,  MOORE MICHAEL ,  FARRIES TIMOTHY ,  COLLINGWOOD TREVOR ,  URNOV FYODOR

Inventor： WOLFFE ALAN P DI ,  MOORE MICHAEL ,  FARRIES TIMOTHY ,  COLLINGWOOD TREVOR ,  URNOV FYODOR

IPC: C12N5/0735 ,  G01N33/50 ,  A01N37/18 ,  A01N43/04 ,  A01N63/00 ,  C07H21/04 ,  C07K1/00 ,  C12N5/00 ,  C12N15/63

CPC classification number: C12N15/85 ,  A61K35/12 ,  C07H21/04 ,  C12N5/0606 ,  C12N15/62 ,  C12N15/63 ,  C12N2501/40 ,  C12N2503/02 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/5073

Abstract: Methods and compositions for modifying stem cells using one or more ZFPs are disclosed. Such methods and compositions are useful for facilitating processes such as, for example, dedifferentiating cells, differentiating stems cells into the desired phenotype, propagating stem cells and/or facilitating cloning.

Abstract translation: 公开了使用一种或多种ZFP修饰干细胞的方法和组合物。 此类方法和组合物可用于促进过程，例如去分化细胞，使干细胞分化成所需表型，增殖干细胞和/或促进克隆。

公开(公告)号：WO2008133938A3

公开(公告)日：2009-04-02

申请号：PCT/US2008005282

申请日：2008-04-24

Applicant: SANGAMO BIOSCIENCES INC ,  DEKELVER RUSSELL ,  GREGORY PHILIP D ,  PASCHON DAVID ,  URNOV FYODOR ,  TAM PHILLIP

Inventor： DEKELVER RUSSELL ,  GREGORY PHILIP D ,  PASCHON DAVID ,  URNOV FYODOR ,  TAM PHILLIP

IPC: C12N15/10 ,  C12N15/90

CPC classification number: C12N15/907 ,  C07K14/435 ,  C07K14/43595 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/1082 ,  C12N15/113 ,  C12N2800/10 ,  C12N2800/30 ,  C12Y301/21004

Abstract: Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into the human PPP1R12C locus, for example, for expression of a polypeptide of interest.

Abstract translation: 本文公开了用于将外源序列靶向整合到人PPP1R12C基因座中的方法和组合物，例如用于表达感兴趣的多肽。

公开(公告)号：WO2005014791A3

公开(公告)日：2006-02-16

申请号：PCT/US2004025407

申请日：2004-08-06

Applicant: SANGAMO BIOSCIENCES INC ,  PABO CARL O ,  URNOV FYODOR ,  HOLMES MICHAEL C ,  MILLER JEFFREY C

Inventor： PABO CARL O ,  URNOV FYODOR ,  HOLMES MICHAEL C ,  MILLER JEFFREY C

IPC: C07K14/47 ,  C12N20060101 ,  C12N9/22 ,  C12N15/10 ,  C07H21/04 ,  A61K35/00 ,  C12N9/00 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63 ,  C12N15/70 ,  C12N15/85 ,  C12Q1/68

CPC classification number: C12N15/10 ,  C07K14/4702 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/902

Abstract: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.

Abstract translation: 本文公开了用于基因组序列的靶向切割，基因组序列的靶向改变以及基因组区域和与该基因组区域同源的外源多核苷酸之间的靶向重组的方法和组合物。 组合物包括包含切割结构域（或切割半结构域）和工程化锌指结构域的融合蛋白以及编码它们的多核苷酸。 用于靶向切割的方法包括将这种融合蛋白或其编码的多核苷酸引入细胞。 靶向重组的方法另外包括将与基因组区域同源的外源多核苷酸引入包含所公开的融合蛋白的细胞中。

公开(公告)号：WO2012012667A2

公开(公告)日：2012-01-26

申请号：PCT/US2011044902

申请日：2011-07-21

Applicant: SANGAMO BIOSCIENCES INC ,  UNIV TEXAS ,  COLLINGWOOD TREVOR ,  COOPER LAURENCE J N ,  GREGORY PHILIP D ,  HOLMES MICHAEL C ,  MILLER JEFFREY C ,  REBAR EDWARD J ,  REIK ANDREAS ,  URNOV FYODOR

Inventor： COLLINGWOOD TREVOR ,  COOPER LAURENCE J N ,  GREGORY PHILIP D ,  HOLMES MICHAEL C ,  MILLER JEFFREY C ,  REBAR EDWARD J ,  REIK ANDREAS ,  URNOV FYODOR

IPC: A61K48/00 ,  A01K67/027 ,  A61P3/00 ,  A61P5/14 ,  A61P5/38 ,  A61P7/02 ,  A61P17/00 ,  A61P17/06 ,  A61P19/00 ,  A61P19/02 ,  A61P19/04 ,  A61P29/00 ,  A61P31/14 ,  A61P37/00 ,  C07K7/06 ,  C12N1/19 ,  C12N5/071 ,  C12N5/0735 ,  C12N5/0775 ,  C12N5/0797 ,  C12N5/10 ,  C12N9/96 ,  C12N15/11 ,  C12N15/63

CPC classification number: C07K14/70539 ,  A61K35/17 ,  C07K2319/81 ,  C12N15/907 ,  C12N2800/80

Abstract: Disclosed herein are methods and compositions for modulating the expression of a HLA locus or for selectively deleting or manipulating a HLA locus or HLA regulator.

Abstract translation: 本文公开了用于调节HLA基因座的表达或用于选择性删除或操纵HLA基因座或HLA调节剂的方法和组合物。

公开(公告)号：WO2012068380A3

公开(公告)日：2012-07-26

申请号：PCT/US2011061201

申请日：2011-11-17

Applicant: SANGAMO BIOSCIENCES INC ,  GREGORY PHILIP D ,  HOLMES MICHAEL C ,  MENDEL MATTHEW C ,  MENG XIANGDONG ,  PASCHON DAVID ,  REIK ANDREAS ,  URNOV FYODOR

Inventor： GREGORY PHILIP D ,  HOLMES MICHAEL C ,  MENDEL MATTHEW C ,  MENG XIANGDONG ,  PASCHON DAVID ,  REIK ANDREAS ,  URNOV FYODOR

IPC: A61K31/00 ,  C07K17/00

CPC classification number: A61K38/1709 ,  A61K35/17 ,  C07K2319/71 ,  C12N9/22

Abstract: Disclosed herein are methods and compositions for modulating expression of a PD1 gene.

Abstract translation: 本文公开了调节PD1基因表达的方法和组合物。

公开(公告)号：WO2008076290A2

公开(公告)日：2008-06-26

申请号：PCT/US2007025455

申请日：2007-12-13

Applicant: DOW AGROSCIENCES LLC ,  SANGAMO BIOSCIENCES INC ,  CAI QIHUA C ,  MILLER JEFFREY ,  URNOV FYODOR ,  SHUKLA VIPULA K ,  PETOLINO JOSEPH F ,  BAKER LISA W ,  GARRISON ROBBI J ,  BLUE RYAN C ,  MITCHELL JON C ,  ARNOLD NICOLE L ,  WORDEN SARAH E

Inventor： CAI QIHUA C ,  MILLER JEFFREY ,  URNOV FYODOR ,  SHUKLA VIPULA K ,  PETOLINO JOSEPH F ,  BAKER LISA W ,  GARRISON ROBBI J ,  BLUE RYAN C ,  MITCHELL JON C ,  ARNOLD NICOLE L ,  WORDEN SARAH E

CPC classification number: C07K14/4702 ,  C07K2319/81 ,  C12N15/8213 ,  C12N15/8243

Abstract: Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described

Abstract translation: 本文公开了包含CCHC锌配位残基的锌指。 还描述了锌指蛋白和包含这些CCHC锌指的融合蛋白以及编码这些蛋白的多核苷酸。 还描述了使用这些蛋白质进行基因编辑和基因调节的方法

公开(公告)号：WO2007014275A3

公开(公告)日：2007-05-24

申请号：PCT/US2006029027

申请日：2006-07-26

Applicant: SANGAMO BIOSCIENCES INC ,  HOLMES MICHAEL C ,  URNOV FYODOR ,  GREGORY PHILIP D ,  REBAR EDWARD J ,  BRENNAN SEAN M

Inventor： HOLMES MICHAEL C ,  URNOV FYODOR ,  GREGORY PHILIP D ,  REBAR EDWARD J ,  BRENNAN SEAN M

IPC: C12P21/06 ,  C07H21/04 ,  C12N15/00

CPC classification number: C12N15/907 ,  A61K48/0008 ,  A61K48/005 ,  A61K48/0058 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/62 ,  C12N15/90

Abstract: Disclosed herein are methods and compositions for targeted integration of a exogenous sequence into a predetermined target site in a genome for use, for example, in protein expression and gene inactivation.

Abstract translation: 本文公开了用于将外源序列靶向整合入基因组中的预定靶位点以用于例如蛋白质表达和基因失活的方法和组合物。

公开(公告)号：WO0183819A9

公开(公告)日：2003-01-03

申请号：PCT/US0113562

申请日：2001-04-27

Applicant: SANGAMO BIOSCIENCES INC

Inventor： WOLFFE ALAN ,  URNOV FYODOR ,  GUSCHIN DMITRY ,  COLLINGWOOD TREVOR ,  LI XIAO-YONG ,  JOHNSTONE BRIAN

IPC: C07K14/47 ,  C12N15/10 ,  C12N15/62 ,  C12N15/63 ,  C12N15/66 ,  C12Q1/68 ,  G06F19/22 ,  G06F19/28

CPC classification number: G06F19/22 ,  C07K14/4702 ,  C07K2319/02 ,  C07K2319/09 ,  C07K2319/24 ,  C07K2319/43 ,  C07K2319/71 ,  C07K2319/75 ,  C07K2319/81 ,  C12N15/1034 ,  C12N15/1051 ,  C12N15/62 ,  C12N15/63 ,  C12N15/66 ,  C12Q1/6811 ,  G06F19/28 ,  C12Q2531/113 ,  C12Q2525/191 ,  C12Q2522/101

Abstract: Methods for the design of exogenous regulatory molecules, comprising a DNA-binding domain and a functional domain, are provided. The methods rely on the identification, isolation and characterization or regulatory DNA sequences obtained on the basis of their accessibility in cellular chromatin.

Abstract translation: 提供了包含DNA结合结构域和功能结构域的外源性调节分子的设计方法。 该方法依赖于基于其在细胞染色质中的可及性而获得的鉴定，分离和鉴定或调节DNA序列。