公开(公告)号：WO2005081699A3

公开(公告)日：2009-04-16

申请号：PCT/US2004033737

申请日：2004-10-12

Applicant: UNIV FLORIDA ,  GOWER LAURIE B ,  OLSZTA MATTHEW J ,  DOUGLAS ELLIOT P ,  MUNISAMY SIVAKUMAR ,  WHEELER DONNA L

Inventor： GOWER LAURIE B ,  OLSZTA MATTHEW J ,  DOUGLAS ELLIOT P ,  MUNISAMY SIVAKUMAR ,  WHEELER DONNA L

IPC: C12N11/14 ,  A61F2/00 ,  A61K38/00 ,  A61L27/24 ,  A61L27/44 ,  A61L27/46 ,  A61L31/12 ,  A61L31/14 ,  C07K17/02 ,  C07K17/14 ,  C12N5/00

CPC classification number: A61L27/24 ,  A61L27/446 ,  A61L27/46 ,  A61L31/127 ,  A61L31/128 ,  A61L31/146 ,  Y10S530/811 ,  Y10S530/812 ,  Y10S530/813 ,  Y10S530/814 ,  Y10S530/815 ,  Y10S530/817

Abstract: The subject invention concerns a composite comprising an organic fluid-swellable, fibrous matrix, such as collagen, and a mineral phase, such as calcium carbonate or phosphate mineral phase, for use as a biomimetic of bone. In another aspect, the subject invention concerns a process for making a composite involving the inclusion of acidic polymers to a supersaturated mineralizing solution, in order to induce an amorphous liquid-phase precursor to the inorganic mineral, which is then absorbed (pulled by capillary action) into the organic matrix. Advantageously, once solidified, a high mineral content can be achieved, with the inorganic mineral crystals embedded within the collagen fibers (intrafibrillarly) and oriented such that they are aligned along the long axes of the fibers of the organic matrix, thereby closely mimicking the natural structure of bone. The present invention further concerns a method of treating a patient suffering from a bone defect by applying a biomimetic composite to the bone defect site.

Abstract translation: 本发明涉及一种复合材料，其包含有机流体 - 可溶胀的纤维基质如胶原和矿物相如碳酸钙或磷酸盐矿物相用作骨仿生体。 在另一方面，本发明涉及一种制备涉及将酸性聚合物包含在过饱和矿化溶液中的复合物的方法，以便诱导无机矿物的无定形液相前体，然后将其吸收（通过毛细作用拉动 ）进入有机基质。 有利地，一旦固化，可以实现高矿物质含量，其中无机矿物晶体嵌入胶原纤维内（纤维内）并定向成使得它们沿着有机基质的纤维的长轴排列，从而紧密地模仿天然 骨结构。 本发明还涉及通过将仿生复合材料应用于骨缺损部位来治疗患有骨缺损的患者的方法。

公开(公告)号：WO02057422A3

公开(公告)日：2003-02-27

申请号：PCT/US0201161

申请日：2002-01-16

Applicant: SCHWARTZ DAVID A

Inventor： SCHWARTZ DAVID A

IPC: A61K47/48 ,  C07D207/44 ,  C07D207/452 ,  C07D207/46 ,  C07D213/82 ,  C07D401/12 ,  C07F7/18 ,  C07K1/107 ,  C07K14/06 ,  C07K14/315 ,  C07K14/52 ,  C07K14/705 ,  C07K16/28 ,  C08G65/329 ,  C08G65/336 ,  C12N9/96 ,  G01N33/543 ,  C07K17/06 ,  C07H21/00 ,  C07K17/08 ,  C12N11/06 ,  C12N11/08

CPC classification number: G01N33/54353 ,  A61K47/61 ,  A61K47/62 ,  A61K47/643 ,  A61K47/645 ,  A61K47/6801 ,  C07D207/452 ,  C07D207/46 ,  C07D213/82 ,  C07D401/12 ,  C07F7/1836 ,  C07K1/1077 ,  C07K14/005 ,  C07K14/315 ,  C07K14/52 ,  C07K14/70503 ,  C07K16/2863 ,  C07K2319/00 ,  C08G65/329 ,  C08G65/336 ,  C08L2203/02 ,  C12N9/96 ,  C12N2710/16722 ,  Y10S530/815 ,  Y10S530/816

Abstract: The present invention is directed to methods for immobilizing natural or synthetic biomolecules to surfaces comprising covalently linking the natural or synthetic biomolecule to a mono- or bi-functional polymer and covalently and/or electrostatically immobilizing the biomolecule/polymer conjugate to an unmodified or modified surface wherein the biomolecule is an oligonucleotide, a polynucleotide, a protein, a glycoprotein, a peptide or a carbohydrate that has been modified to incorporate a single or plurality of nucleophilic groups comprising an aliphatic or aromatic amino, thiol, hydrazine, thiosemicarbazide, hydrazide, thiocarbazide, carbazide, aminooxy, a derivative of 2-hydrazinopyridine or aminoxyacetic acid or a single or plurality of electrophilic groups comprising an aliphatic or aromatic aldehyde, ketone, epoxide, isocyanate, isothiocyanate, succinimidyl ester or cyanuric chloride or a linkable aromatic aldehyde or ketone and the surface has been modified to possess either neutral, cationic or anionic groups or a combination neutral, anionic and/or cationic moieties.

Abstract translation: 本发明涉及将天然或合成生物分子固定在表面上的方法，包括将天然或合成生物分子共价连接到单功能或双官能聚合物上并共价和/或静电将生物分子/聚合物缀合物固定在未修饰或修饰的表面上 其中所述生物分子是经修饰以掺入单个或多个亲核基团的寡核苷酸，多核苷酸，蛋白质，糖蛋白，碳水化合物，其包含脂族或芳族氨基，硫醇，肼，氨基硫脲，酰肼，硫代卡巴肼 ，卡巴肼，氨基氧基，2-肼基吡啶或氨基乙酸的衍生物或包含脂族或芳族醛，酮，环氧化物，异氰酸酯，异硫氰酸酯，琥珀酰亚胺酯或氰尿酰氯或可连接的芳族醛或酮的单个或多个亲电子基团， 表面已被修改为拥有neu 阳离子或阴离子基团或中性，阴离子和/或阳离子部分的组合。

公开(公告)号：WO2002096979A2

公开(公告)日：2002-12-05

申请号：PCT/US2002/016672

申请日：2002-05-29

Applicant: POREX TECHNOLOGIES CORPORATION

Inventor： YAO, Li ,  GREENE, George, Warren, IV ,  MAO, Guoqiang ,  LI, Xingguo

IPC: C08J

CPC classification number: A61L31/128 ,  A61L27/446 ,  A61L27/56 ,  A61L31/146 ,  Y10S530/811 ,  Y10S530/815 ,  Y10S530/816 ,  Y10T428/249953 ,  Y10T428/249986 ,  Y10T428/31855

Abstract: The invention relates to porous polymeric materials, methods of making them, and applications in medical devices. A specific embodiment of the invention encompasses a material comprising a porous polyolefin substrate containing inclusions of a material to which chemical or biological moieties are attached directly or via a spacer.

Abstract translation: 本发明涉及多孔聚合材料，其制备方法以及在医疗装置中的应用。 本发明的具体实施方案包括含有多孔聚烯烃基材的材料，该多孔聚烯烃基材含有化学或生物部分直接或通过间隔物连接的材料的夹杂物。

公开(公告)号：WO0070088A3

公开(公告)日：2002-03-28

申请号：PCT/US0012729

申请日：2000-05-10

Applicant: BECKMAN COULTER INC

Inventor： MATSON ROBERT S ,  MILTON RAYMOND C

IPC: G01N33/53 ,  C12N11/08 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/543 ,  G01N37/00 ,  B01J19/00 ,  C08F8/18

CPC classification number: G01N33/54353 ,  C12Q1/6834 ,  Y10S530/81 ,  Y10S530/811 ,  Y10S530/815

Abstract: A method of attaching unmodified biopolymers, particularly, unmodified polynucleotides, directly to a solid support is provided. The method includes the steps of (a) providing unmodified biopolymers; (b) providing a solid support having at least one surface comprising pendant acyl fluoride functionalities; and (c) contacting the unmodified biopolymers with the solid support under a condition sufficient for allowing the attachment of the biopolymers to the solid support. The unmodified biopolymers may be nucleic acids, polypeptides, proteins, carbohydrates, lipids and analogues thereof. The unmodified polynucleotides may be DNA, RNA or synthesized oligonucleotides. The DNA may be single or double stranded. A device including a solid support and unmodified biopolymers attached to the solid support by reaction with the pendant acyl fluoride functionalities of the solid support is also provided. The methods and devices of the present invention may be used in performing hybridization assays and immunoassays.

Abstract translation: 提供了将未改性的生物聚合物，特别是未修饰的多核苷酸直接连接到固体支持物上的方法。 该方法包括以下步骤：（a）提供未改性的生物聚合物; （b）提供具有至少一个表面的固体支持物，所述表面包含酰基侧酰氟官能团; 和（c）在足以允许生物聚合物附着到固体支持物的条件下使未改性的生物聚合物与固体支持物接触。 未修饰的生物聚合物可以是核酸，多肽，蛋白质，碳水化合物，脂质及其类似物。 未修饰的多核苷酸可以是DNA，RNA或合成的寡核苷酸。 DNA可以是单链或双链。 还提供了包括固体支持物和通过与固体支持物的酰基侧酰氟官能团反应连接到固体支持物上的未改性生物聚合物的装置。 本发明的方法和装置可用于进行杂交测定和免疫测定。

公开(公告)号：WO0214078A2

公开(公告)日：2002-02-21

申请号：PCT/US0125429

申请日：2001-08-14

Applicant: SURFACE LOGIX INC ,  SCHUELLER OLIVIER ,  KIM ENOCH ,  WHITESIDES GEORGE

Inventor： SCHUELLER OLIVIER ,  KIM ENOCH ,  WHITESIDES GEORGE

IPC: B05D1/28 ,  G03F7/00 ,  B41K1/00

CPC classification number: B82Y10/00 ,  B05D1/283 ,  B82Y30/00 ,  B82Y40/00 ,  G03F7/0002 ,  Y10S530/81 ,  Y10S530/811 ,  Y10S530/812 ,  Y10S530/815

Abstract: A deformable stamp for patterning a surface. The stamp can be placed in contact with an entire 3-dimensional object, such as a rod, in a single step. The stamp can also be used to pattern the inside of a tube or rolled over a surface to form a continous pattern. The stamp may also be used for fluidic patterning by flowing material through channels defined by raised and recessed portions in the surface of the stamp as it contacts the substrate. The stamp may be use d to deposit self-assembled monolayers, biological materials, metals, polymers, ceramics, or a variety of other materials. The patterned substrates may be used in a variety of engineering and medical applications.

Abstract translation: 用于图案化表面的可变形印模。 邮票可以在一个步骤中与整个三维物体（例如杆）接触。 印模也可以用于对管的内部进行图案化或者在表面上滚动以形成连续图案。 当印模接触基板时，印模也可以通过流动材料流过印模表面中由凸起和凹陷部分限定的通道而用于流体图案化。 邮票可以用于沉积自组装单层，生物材料，金属，聚合物，陶瓷或各种其他材料。 图案化的基底可以用于各种工程和医疗应用。

公开(公告)号：WO0007543A8

公开(公告)日：2000-06-08

申请号：PCT/US9917648

申请日：1999-08-04

Applicant: WATSON LAB INC UTAH ,  PRAKASH RAMESH K ,  CLEMENS CHRISTOPHER M

Inventor： PRAKASH RAMESH K ,  CLEMENS CHRISTOPHER M

IPC: A61K38/00 ,  A61K45/06 ,  A61K47/48 ,  A61P35/02 ,  A61K38/08 ,  C07K4/00

CPC classification number: A61K47/6425 ,  A61K47/65 ,  Y10S530/812 ,  Y10S530/815 ,  Y10S930/141

Abstract: A composition for intracellular delivery of a chemical agent into a target receptor and/or interleukin-2-receptor-bearing cell, e.g. an activated T cell and cancer cell, includes a chemical agent, at least one copy of target-receptor binding and/or an interleukin-2-receptor-binding and endocytosis-inducing ligand coupled to a water soluble polymer. The ligand binds to a target receptor and/or IL-2 receptor on the target receptor and/or IL-2-receptor-bearing cell and elicits endocytosis of the composition. The composition also optionally includes a biodegradable spacer for coupling the chemical agent and the ligand to the polymer. Chemical agents can include cytotoxins, transforming nucleic acids, gene regulators, labels, antigens, drugs, and the like. A preferred water soluble polymer is polyalkylene oxide, such as polyethylene glycol and polyethylene oxide, and activated derivatives thereof. The composition can further comprise a carrier such as another water soluble polymer, liposome, or particulate. Methods of using these compositions for delivering a chemical agent in vivo or in vitro are also disclosed. A method of detecting a disease, such as cancer, T-cell lymphocytic leukemia, T-cell acute lymphoblastic leukemia, peripheral T-cell lymphoma, Hodgkin's disease, and non-Hodgkin's lymphoma, associated with elevated levels of soluble target receptor and/or IL-2 receptor is also disclosed.

公开(公告)号：WO00015272A1

公开(公告)日：2000-03-23

申请号：PCT/NL1999/000572

申请日：1999-09-14

IPC: A61K8/02 ,  A61K8/72 ,  A61K47/32 ,  A61K47/48 ,  A61L27/00 ,  A61L27/38 ,  A61L27/52 ,  C08B11/20 ,  C08B37/00 ,  C08G73/02 ,  C08G85/00 ,  C08J3/20 ,  C08J5/00 ,  C08K5/00 ,  C08L33/02 ,  C08L71/02 ,  C08L79/02 ,  C08L89/00 ,  C12N1/16 ,  C12N11/08 ,  C12R1/645 ,  A61F2/14 ,  C12N5/00

CPC classification number: A61L27/3843 ,  A61L27/52 ,  C08B11/20 ,  C08B37/0075 ,  C08G73/0206 ,  C08G85/00 ,  C12N11/08 ,  Y10S530/812 ,  Y10S530/815 ,  Y10S530/817

Abstract: Process for providing immobilized living cells, said process comprising the steps of: mixing together under reactive conditions a polymer and an activator for said polymer, such as a cross-linking agent, so that the polymer is activated, whereafter a living cell is separately added to the activated polymer, so that the living cell is not exposed to substantially unreacted activator, whereby the living cell is immobilized within the activated polymer.

Abstract translation: 用于提供固定的活细胞的方法，所述方法包括以下步骤：在反应条件下将聚合物和所述聚合物如交联剂的活化剂混合在一起，使得聚合物被活化，之后分开加入活细胞 活化聚合物，使得活细胞不暴露于基本上未反应的活化剂，由此将活细胞固定在活化聚合物内。

公开(公告)号：WO99029759A1

公开(公告)日：1999-06-17

申请号：PCT/US1998/026402

申请日：1998-12-11

IPC: C08G65/26 ,  C07K1/107 ,  C08G65/329 ,  C08G63/00 ,  C08G63/02 ,  C08G63/68 ,  C08G69/00 ,  C08G69/04 ,  C08G69/26 ,  C08G69/42 ,  C08G73/00 ,  C08G75/00 ,  C08G79/00

CPC classification number: C07K1/1077 ,  C08G65/329 ,  Y10S530/815

Abstract: A biocompatible, heterofunctional, star-shaped poly(ethylene glycol) is described. Methods of making the heterofunctional star-shaped poly(ethylene glycol) and using it for conjugation with proteins are also described.

Abstract translation: 描述了生物相容的异官能星形聚（乙二醇）。 还描述了制备异功能星形聚（乙二醇）并将其用于与蛋白质缀合的方法。

公开(公告)号：WO99017139A1

公开(公告)日：1999-04-08

申请号：PCT/US1998/020081

申请日：1998-09-25

IPC: G01N21/77 ,  G01N21/85 ,  G02B6/00

CPC classification number: G01N21/7703 ,  G01N21/8507 ,  Y10S530/811 ,  Y10S530/815

Abstract: Optical fiber sensors and fiberless optical sensors for measuring analytes, and in particular nitric oxide, are described utilizing metals, and more particularly, metal colloids. Proteins (or fragments thereof) with selective binding are immobilized on metal particles. The proteins may be dye-labeled for increased sensitivity.

Abstract translation: 使用金属，更具体地，金属胶体描述用于测量分析物，特别是一氧化氮的光纤传感器和无纤维光学传感器。 具有选择性结合的蛋白质（或其片段）固定在金属颗粒上。 蛋白质可能被染色标记以提高灵敏度。

公开(公告)号：WO1998001555A2

公开(公告)日：1998-01-15

申请号：PCT/US1997012244

申请日：1997-07-09

Applicant: AMGEN INC. ,  FISHER, Eric, F. ,  EDWARDS, Carl, K. ,  KIEFT, Gary, L.

Inventor： AMGEN INC.

IPC: C12N15/12

CPC classification number: C07K14/7151 ,  A01K67/027 ,  A01K2217/05 ,  A01K2267/0325 ,  A61K38/00 ,  A61K47/60 ,  C07K14/705 ,  C07K2319/00 ,  Y10S530/815

Abstract: Disclosed are proteins, referred to as tumor necrosis factor binding proteins, that modulate the activity of tumor necrosis factor. Also disclosed are processes for obtaining the tumor necrosis binding proteins by recombinant genetic engineering techniques.

Abstract translation: 公开了调节肿瘤坏死因子活性的蛋白质，称为肿瘤坏死因子结合蛋白。 还公开了通过重组基因工程技术获得肿瘤坏死结合蛋白的方法。