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    REAL-TIME ELECTRONIC SEQUENCING
    10.
    发明公开
    REAL-TIME ELECTRONIC SEQUENCING 有权
    电子实时序列

    公开(公告)号：EP2994544A4

    公开(公告)日：2017-01-25

    申请号：EP14794438

    申请日：2014-05-05

    申请人： PACIFIC BIOSCIENCES CALIFORNIA INC

    发明人： TURNER STEPHEN ,  HANES JEREMIAH ,  BJORNSON KEITH

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q1/6823 ,  G01N27/227 ,  C12Q2521/101 ,  C12Q2521/543 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2563/116 ,  C12Q2563/157 ,  C12Q2565/607

    摘要： Real time electronic sequencing methods, devices, and systems are described. Arrays of nanoscale electronic elements comprising capacitive devices with one or two electrodes, or arrays of nanoFET devices are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the nanoscale electronic elements or to the substrate proximate to the nanoscale electronic element. A sequencing reaction mixture comprising nucleotide analogs having impedance labels, capacitive labels, or conductivity labels is introduced to the array of nanoscale electronic elements comprising capacitive devices or nanoFETs under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by identifying the types of labels of the nucleotide analogs that are incorporated into the growing strand using measured impedance, conductivity, or capacitance.