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    APPARATUS AND METHOD FOR PREPARATIVE SCALE PURIFICATION OF NUCLEIC ACIDS
    1.
    发明公开
    APPARATUS AND METHOD FOR PREPARATIVE SCALE PURIFICATION OF NUCLEIC ACIDS 审中-公开
    VORRICHTUNG UND VERFAHREN ZUR REINIGUNG VONNUKLEINSûURENIM PRUDPARATIVEN MASSSTAB

    公开(公告)号：EP1554398A4

    公开(公告)日：2005-12-14

    申请号：EP03749647

    申请日：2003-09-12

    申请人： VALENTIS INC

    发明人： AU-YEUNG JACKIE ,  BUSSEY LEE B

    IPC分类号： C12N15/00 ,  B01D20060101 ,  B03D3/06 ,  C07H19/00 ,  C07H21/02 ,  C07H21/04 ,  C12M1/00 ,  C12M1/04 ,  C12N1/20 ,  C12N15/09 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12N15/101 ,  B03D3/06 ,  C12N1/066 ,  C12N15/1003 ,  C12N15/1017 ,  C12Q1/6806 ,  C12Q2527/119 ,  C12Q2565/137

    摘要： Apparatus and methods are described for pharmaceutical grade manufacture extrachromosomal nucleic acids from cell lysates using flotation to separate and eliminate undesired insoluble cellular debris including chromosomal DNA from the lysates. A gas is introduced to controllably generate bubbles that reduce the density of the cell debris and create a buoyant flocculent phase that can be readily separated from, and thus provide, a substantially clarified fluid lysate phase that is enriched in extrachromosomal DNA but substantially depleted of cellular proteins and chromosomal DNA.

    摘要翻译： 描述了使用浮选从细胞裂解物中药物级制造染色体外核酸以分离和消除不希望的不溶性细胞碎片（包括来自裂解物的染色体DNA）的装置和方法。 引入气体以可控制地产生气泡，所述气泡降低细胞碎片的密度并产生漂浮的絮凝相，所述漂浮的絮凝相可以容易地从基本澄清的流体裂解物相中分离并因此提供富含染色体外DNA但基本上耗尽细胞 蛋白质和染色体DNA。

    METHODS FOR PURIFYING NUCLEIC ACIDS
    5.
    发明公开
    METHODS FOR PURIFYING NUCLEIC ACIDS 审中-公开
    对于纯化核酸的方法

    公开(公告)号：EP1098966A1

    公开(公告)日：2001-05-16

    申请号：EP99932304.1

    申请日：1999-07-07

    申请人： Valentis Inc.

    发明人： BRIDENBAUGH, Robert ,  DANG, Warren ,  BUSSEY, Lee

    IPC分类号： C12N15/10 ,  C12P19/34

    CPC分类号： C07H1/06 ,  C07H21/00 ,  C12N15/1003 ,  C12N15/1017

    摘要： Methods are provided for producing highly purified compositions of nucleic acids by using a highly streamlined and readily automated process. The methods use static mixers for lysing cells and precipitating debris, followed by centrifugation and ion exchange chromatography. The process may include a purification step using tangential flow ultrafiltration. A scaleable process for producing pharmaceutical grade plasmid DNA, useful for gene therapy, is provided.

    EXPRESSION PLASMIDS FOR MULTIEPITOPE NUCLEIC ACID-BASED VACCINES
    6.
    发明公开
    EXPRESSION PLASMIDS FOR MULTIEPITOPE NUCLEIC ACID-BASED VACCINES 审中-公开
    表达质粒多表位的DNA疫苗

    公开(公告)号：EP1030927A1

    公开(公告)日：2000-08-30

    申请号：EP98957671.5

    申请日：1998-11-09

    申请人： Valentis Inc.

    发明人： LEDEBUR, Harry, C., Jr. ,  NORDSTROM, Jeffrey, L.

    IPC分类号： C12N15/86 ,  A61K39/00

    CPC分类号： A61K39/00 ,  A61K2039/51 ,  A61K2039/53 ,  C12N15/85 ,  C12N2830/42 ,  C12N2840/203 ,  C12N2840/44

    摘要： Improved plasmids and methods for nucleic acid-based vaccines. The use of epitopes, small immunologically relevant protein sequences that are capable of inducing both cellular and humoral responses that result in a protective or therapeutic immune response against large and complex pathogens for incorporation into nucleic acid-based vaccines. The structures and characteristics of gene expression systems that are capable of expressing multiple epitopes. A method of genetic immunization comprising the step of presenting multiple epitopes to an organism in need of said immunization. A plasmid for expression of multiple epitopes comprising a nucleic acid sequence encoding multiple epitopes, wherein each of said epitopes is capable of creating an immune response. A multivalent expression system as shown in Figure 8 and selected from the group consisting of two plasmids, two genes, IRES, and alternative splicing and a method of making a plasmid producing the appropriate nucleic acid sequence.

    CO-LYOPHILIZED COMPLEX COMPRISING A NUCLEIC ACID VECTOR AND A FORMULATING AGENT
    10.
    发明授权
    CO-LYOPHILIZED COMPLEX COMPRISING A NUCLEIC ACID VECTOR AND A FORMULATING AGENT 有权
    包含核酸载体和制剂的共冻干复合物

    公开(公告)号：EP1104309B1

    公开(公告)日：2006-05-03

    申请号：EP99939113.9

    申请日：1999-08-10

    申请人： Valentis Inc.

    发明人： BRUNO, Maria ,  TAGLIAFERRI, Jenna ,  LAWSON, Luke ,  LOGAN, Mark, J. ,  MUMPER, Russ

    IPC分类号： A61K48/00 ,  C12N15/87

    CPC分类号： A61K48/00 ,  C12N15/87 ,  Y10S977/792 ,  Y10S977/80 ,  Y10S977/801 ,  Y10S977/906 ,  Y10S977/907

    摘要： The present invention, as noted above, relates generally to the incorporation of plasmid into a conventional dosage form, and more particularly to the production of a single-vial, homogenized, plasmid/polymer complex with desirable physical characteristics. Methods of making, storing and using such a complex are also provided and described in detail below. Such products and methods will provide more convenient and cost-effective complexes, which will be protected against chemical degradation and/or physical aggregation of its components and will provide for relative ease of administration. Thus, the present invention provides a more efficient complex for plasmid delivery and a method of incorporation of that plasmid into a conventional dosage form.

    摘要翻译： 如上所述，本发明一般涉及将质粒掺入常规剂型，更具体地涉及生产具有所需物理特性的单瓶均质的质粒/聚合物复合物。 下面还提供并且详细描述制造，储存和使用这种复合物的方法。 这样的产品和方法将提供更方便和成本有效的复合物，其将被保护免受化学降解和/或其组分的物理聚集，并且将提供相对容易的施用。 因此，本发明提供了用于质粒递送的更有效的复合物和将该质粒并入常规剂型的方法。