-
1.发明公开METHOD FOR GENERATING SPLIT, NON-TRANSFERABLE GENES THAT ARE ABLE TO EXPRESS AN ACTIVE PROTEIN PRODUCT 有权用于生产，这使是一个积极的蛋白产物表达分离的非传染性基因
公开(公告)号：EP1183346A1
公开(公告)日：2002-03-06
申请号：EP00932712.3
申请日：2000-05-23
发明人： XU, Ming-Qun , EVANS, Thomas, C. , PRADHAN, Sriharsa , COMB, Donald, G. , PAULUS, Henry , SUN, Luo , CHEN, Lixin , GHOSH, Inca
CPC分类号： C12N9/1029 , C07K14/43595 , C07K2319/00 , C07K2319/055 , C07K2319/06 , C07K2319/08 , C07K2319/09 , C07K2319/32 , C07K2319/75 , C07K2319/92 , C12N9/1092 , C12N9/1252 , C12N9/88 , C12N15/10 , C12N15/62 , C12N15/8214 , C12N15/8216 , C12N15/8241 , C12N15/8242 , C12N15/8257 , C12N15/8275 , C12N15/8278 , C12P21/02
摘要： A new type of transgene system is disclosed which allows efficient protein expression in a target host such as a plant, but avoids the undesirable result of the migration of the transgene into related host systems and/or to the environment via the pollen. The methods described herein may also be applied to the expression of virtually any protein of interest (e.g. a toxic protein) in eukaryotic (yeast, insect, mammalian cells, etc.) and prokaryotic (E. coli, etc.) organisms.
-
-
公开(公告)号：EP1805327B1
公开(公告)日：2013-10-09
申请号：EP05817526.6
申请日：2005-10-20
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6844 , C12Q1/6848 , C12Q2521/501 , C12Q2521/301 , C12Q2521/514 , C12Q2521/101 , C12Q2521/331
-
4.发明授权METHOD FOR GENERATING SPLIT, NON-TRANSFERABLE GENES THAT ARE ABLE TO EXPRESS AN ACTIVE PROTEIN PRODUCT 有权用于生产，这使是一个积极的蛋白产物表达分离的非传染性基因
公开(公告)号：EP1183346B1
公开(公告)日：2007-10-31
申请号：EP00932712.3
申请日：2000-05-23
发明人： XU, Ming-Qun , EVANS, Thomas, C. , PRADHAN, Sriharsa , COMB, Donald, G. , PAULUS, Henry , SUN, Luo , CHEN, Lixin , GHOSH, Inca
CPC分类号： C12N9/1029 , C07K14/43595 , C07K2319/00 , C07K2319/055 , C07K2319/06 , C07K2319/08 , C07K2319/09 , C07K2319/32 , C07K2319/75 , C07K2319/92 , C12N9/1092 , C12N9/1252 , C12N9/88 , C12N15/10 , C12N15/62 , C12N15/8214 , C12N15/8216 , C12N15/8241 , C12N15/8242 , C12N15/8257 , C12N15/8275 , C12N15/8278 , C12P21/02
摘要： A new type of transgene system is disclosed which allows efficient protein expression in a target host such as a plant, but avoids the undesirable result of the migration of the transgene into related host systems and/or to the environment via the pollen. The methods described herein may also be applied to the expression of virtually any protein of interest (e.g. a toxic protein) in eukaryotic (yeast, insect, mammalian cells, etc.) and prokaryotic (E. coli, etc.) organisms.
-
公开(公告)号：EP4437093A2
公开(公告)日：2024-10-02
申请号：EP22843953.5
申请日：2022-11-22
IPC分类号： C12N9/22
CPC分类号： C12N9/22 , C12Y305/04005 , C12N9/78 , C12Q1/6806 , C12Q1/6869
-
公开(公告)号：EP4103702A1
公开(公告)日：2022-12-21
申请号：EP21710761.4
申请日：2021-02-12
发明人： GARDNER, Andrew, F. , ZATOPEK, Kelly, M. , EVANS, Thomas, C. , ALPASLAN, Ece , SAUGUET, Ludovic
IPC分类号： C12N9/12
-
公开(公告)号：EP2836603A1
公开(公告)日：2015-02-18
申请号：EP13775998.1
申请日：2013-04-04
IPC分类号： C12P19/34
CPC分类号： C12Q1/6848 , C12N9/1252 , C12Q2525/101 , C12Q2525/301 , C12Q2527/127 , C12Q2549/101
摘要： Compositions and methods are provided for inhibiting a polymerase from replicating non target DNA at a temperature below the amplification reaction temperature. The inhibitor is a synthetic nucleic acid which is single stranded but folds to form at least one double stranded region designed to melt at a temperature which is lower than the amplification reaction temperature, and at least one single stranded region where the single stranded region at the 5' end contains at least one uracil or inosine and optionally a sequence at the 3' end contains one or more derivative nucleotide or linkages.
-
公开(公告)号：EP2935624A1
公开(公告)日：2015-10-28
申请号：EP13822026.4
申请日：2013-12-19
IPC分类号： C12Q1/68
CPC分类号： C12N9/93 , C12N9/00 , C12Q1/6853 , C12Q1/6862 , C12Q2521/501 , C12Q2561/125 , C12Y605/01003
摘要： Compositions and methods are provided for ligating polynucleotides having a length that is greater than 8 nucleotides on an RNA splint. The ligation reaction provides consistent results in high or low ATP concentrations. The reaction can occur rapidly and is generally at least 10 fold more efficient than T4DNA ligase under optimal conditions for T4DNA ligase and the reaction time is less than 6 hours for example, less than 1 hour.
摘要翻译： 提供组合物和方法用于在RNA夹板上连接长度大于8个核苷酸的多核苷酸。 连接反应在高或低ATP浓度下提供一致的结果。 该反应可以快速发生，并且在T4DNA连接酶的最佳条件下通常比T4DNA连接酶的效力至少高10倍，反应时间小于6小时，例如小于1小时。
-
公开(公告)号：EP2010678A2
公开(公告)日：2009-01-07
申请号：EP07755155.4
申请日：2007-04-11
发明人： EVANS, Thomas, C. , SLATKO, Barton , CHEN, Lixin , VAISVILA, Romaldas , GUAN, Chudi , KUCERA, Rebecca
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2521/514 , C12Q2521/501 , C12Q2521/301 , C12Q2525/119
摘要： Methods and compositions are provided for repairing a polynucleotide so that it can be copied with improved fidelity and/o yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a DNA ligase and an effective amount of at least one endonuclease as well as a cofactor selected from NAD+ or ATP.
-
10.发明公开EXPRESSION, SECRETION AND PURIFICATION OF RECOMBINANT BOVINE SERUM ALBUMIN (RBSA) AND USES THEREOF 审中-公开表达，分泌和重组牛血清白蛋白的清洁（RBSA）及其用途
公开(公告)号：EP2001905A1
公开(公告)日：2008-12-17
申请号：EP07754440.1
申请日：2007-03-30
IPC分类号： C07K14/765
CPC分类号： C07K14/765 , C12N9/22 , C12N9/96 , C12N15/815
摘要： A recombinant BSA (rBSA) that (a) substantially lacks deoxyribonuclease activity as determined by incubating rBSA with linear DNA overnight and gel electrophoresis, (b) lacks animal viruses associated with animal-derived cell growth supplements; and (c) is capable of stabilizing DNA proteins is provided. Methods for making the rBSA and using it to stabilize enzymes are also provided.
-
-
-
-
-
-
-
-
-
搜索结果
17 条记录 (耗时 0.014 秒)
