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公开(公告)号：EP2395358B1
公开(公告)日：2017-03-29
申请号：EP11003760.3
申请日：2005-07-29
申请人： Promega Corporation
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公开(公告)号：EP2327768B1
公开(公告)日：2015-09-09
申请号：EP11155576.9
申请日：2007-04-02
申请人： Promega Corporation
发明人： Binkowski, Brock , Fan, Frank , Wigdal, Susan , Wood, Keith V. , Wood, Monika G.
CPC分类号： C12N9/0069 , C12N9/0004 , C12Q1/66 , C12Y113/12007 , G01N33/84
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公开(公告)号：EP3409764B1
公开(公告)日：2020-04-15
申请号：EP18184699.9
申请日：2010-05-03
申请人： Promega Corporation
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公开(公告)号：EP3409764A1
公开(公告)日：2018-12-05
申请号：EP18184699.9
申请日：2010-05-03
申请人： Promega Corporation
发明人： Encell, Lance P. , Wood, Keith V. , Wood, Monika G. , Hall, Mary P. , Otto, Paul , Vidugiris, Gediminas , Zimmerman, Kristopher
摘要： The present invention discloses a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
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5.发明公开Thermostable luciferases from Photuris pennsylvanica and Pyrophorus plagiophthalamus and methods of production 审中-公开Photuris pennsylvanica和Pyrophorus plagiophthalamus的热稳定萤光素酶及其生产方法
公开(公告)号：EP1630231A2
公开(公告)日：2006-03-01
申请号：EP05076634.4
申请日：1999-12-22
申请人： PROMEGA CORPORATION
发明人： Wood, Monika G. , Wood, Keith V. , Hall, Mary P.
CPC分类号： C12N9/0069 , C07K2319/00 , C12N15/10 , Y10S977/898
摘要： Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50°C, cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
摘要翻译： 公开了具有极大增加的热稳定性的萤光素酶，例如在50℃下至少半衰期为2小时，编码新型萤光素酶的cDNA和转化以表达萤光素酶的宿主。 产生萤光素酶的方法包括递归诱变。 萤光素酶以常规方法使用，一些使用试剂盒。
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公开(公告)号：EP4169935A1
公开(公告)日：2023-04-26
申请号：EP22196592.4
申请日：2014-03-13
申请人： Promega Corporation
发明人： Dixon, Andrew S. , Encell, Lance P. , Hall, Mary P. , Wood, Keith V. , Wood, Monika G. , Schwinn, Marie , Binkowski, Brock , Zegzouti, Hicham , Nath, Nidhi , Mondal, Subhanjan , Goueli, Said , Meisenheimer, Poncho , Kirkland, Thomas , Unch, James , Pulukkunat, Dileep K. , Robers, Matthew B. , Dart, Melanie , Machleidt, Thomas
IPC分类号： C07K14/435 , G01N33/50
摘要： Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
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7.发明公开
公开(公告)号：EP3181687A1
公开(公告)日：2017-06-21
申请号：EP17154777.1
申请日：2010-05-03
申请人： Promega Corporation
发明人： Encell, Lance P. , Wood, Keith V. , Wood, Monika G. , Hall, Mary , Otto, Paul , Vidugiris, Gediminas , Zimmerman, Kristopher
CPC分类号： C12Q1/66 , C12N9/0004
摘要： The present invention discloses a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
摘要翻译： 本发明公开了修饰的萤光素酶多肽。 修饰的萤光素酶多肽与野生型Oplophorus萤光素酶具有至少60％的氨基酸序列同一性，并且在对应于SEQ ID NO：1的野生型Oplophorus萤光素酶中的氨基酸的位置上包含至少一个氨基酸取代。 相对于野生型Oplophorus萤光素酶，经修饰的萤光素酶多肽具有增强的发光，增强的信号稳定性和增强的蛋白质稳定性中的至少一种。
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公开(公告)号：EP2492342A1
公开(公告)日：2012-08-29
申请号：EP12153529.8
申请日：2007-10-30
申请人： Promega Corporation
发明人： Darzins, Aldis , Zhao, Kate Qin , Encell, Lance P. , Ohana, Rachel Friedman , Otto, Paul , Vidugiris, Gediminas , Wood, Keith V. , Wood, Monika G. , Zimmerman, Kris , Slater, Michael R.
CPC分类号： C12N9/14 , C12N9/96 , C12N11/00 , C12Q1/34 , C12Y308/01 , G01N33/581
摘要： The invention provides a muttant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
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公开(公告)号：EP3744834A1
公开(公告)日：2020-12-02
申请号：EP20169057.5
申请日：2010-05-03
申请人： Promega Corporation
发明人： Encell, Lance P. , Wood, Keith V. , Wood, Monika G. , Hall, Mary P. , Otto, Paul , Vidugiris, Gediminas , Zimmerman, Kristopher
摘要： The present invention discloses a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
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公开(公告)号：EP2990478B1
公开(公告)日：2017-04-05
申请号：EP15186652.2
申请日：2010-05-03
申请人： Promega Corporation
发明人： Encell, Lance P. , Wood, Keith V. , Wood, Monika G. , Hall, Mary , Otto, Paul , Vidugiris, Gediminas , Zimmerman, Kristopher
CPC分类号： C12Q1/66 , C12N9/0004
摘要： The present invention discloses a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
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