摘要:
A fluidic centripetal apparatus (1) for testing components of a biological material in a fluid, said fluidic centripetal device having a shape adapted to be received within a rotatable holder, said rotatable holder having a center of rotation and an outer edge. The apparatus comprising: a fluidic component layer (3) having fluidic features on at least a front face, said fluidic features including an entry channel for circulating said fluid, said entry channel (302) being coupled to a intake (5) receptacle outlet; a retention chamber (403), said retention chamber being coupled to said entry channel via said intake receptacle outlet for receiving said fluid into said retention chamber; a container wholly provided in said retention chamber and containing a liquid diluent, and a bottom component layer (4) bonded to a rear of said fluidic component layer thereby creating a fluidic network through which said fluid flows under centripetal force.
摘要:
Method and apparatus for amplifying a target nucleic acid sequence of a reaction mixture in a Polymerase Chain Reaction (PCR). The method includes contacting the reaction mixture with EMR frequency absorbing particles formed from a material having a transition metal, transition metal oxide or a transition metal hydroxide, or a nitride, a phosphide or an arsenide of a Group III metal doped with the transition metal or a transition metal oxide, or silicon dioxide doped with the transition metal, transition metal oxide, or transition metal hydroxide; and irradiating the EMR absorbing particles with EMR having a frequency of about 200 kHz to 500 THz to amplify the target nucleic acid sequence, wherein the Group III metal is any one of Al, Ga, and In, and the transition metal is any one of Mn, Fe, Co and Cu.
摘要:
A disposable cassette for detecting nucleic acids or performing other assays. The cassette can be inserted into a base station during use. The cassette has numerous features to ensure correct operation of the device under gravity, such as vent pockets for enabling the flow of sample fluid from one chamber to the next when the vent pocket is unsealed. The vent pockets have protrusions to help prevent accidental resealing. The cassette also can have a gasket to ensure free air movement between open vent pockets. A flexible circuit with patterned metallic electrical components disposed on a heat stable material can be in direct contact with fluid in the chambers and has resistive heating elements aligned with the vent pockets and the chambers. The detection chamber, which houses a lateral flow detection strip can have a space below the strip that has sufficient capacity to accommodate an entire volume of the sample fluid entering the detection chamber at a height that enables the fluid to flow up the detection strip by capillary action without flooding or otherwise bypassing regions of the detection strip. The space can also contain detection particles. Recesses in in the cassette channels or chambers can have structures such as ridges or grooves to direct fluid flow to enhance rehydration of lyophilized reagents disposed in the recess. Flow diverters in the chambers can reduce the flow velocity of the sample fluid and increase the effective fluid flow path length, enabling more accurate control of fluid flow in the cassette.
摘要:
Disclosed is a chemiluminescence measurement apparatus (10) that includes: a support member (31) configured to support a cartridge (20) for measuring a test substance contained in a specimen by chemiluminescence measurement; a motor (40) configured to rotate the support member (31) so as to rotate the cartridge such that a process required for the chemiluminescence measurement proceeds in the cartridge (20); and a light receiver (70) configured to receive light generated by chemiluminescence in the cartridge (20) that is supported by the support member (31) rotated by the motor (40). The cartridge supported by the support member and a light receiving surface of the light receiver are disposed inside a dark space (81) surrounded by a light-shielding portion (80), and the motor (40) is disposed outside the dark space (80).
摘要:
An exhaust device (100), in particular for laboratory areas, comprising: a housing with side walls and a cover wall (170) enclosing a working chamber (180), wherein at least one of the side walls comprises a moveable window slide (150); a controllable suction device (120; 120') to guide air from the working chamber (180) via at least one exhaust air connection (123; 123') of an exhaust air system, wherein the suction device (120; 120') comprises at least two air inlet openings (124, 125, 126; 124', 125', 126') which are separated from one another and the volume flows of which can be adjusted and controlled independently from one another, wherein the volume flows of the air inlet openings (124, 125, 126; 124', 125', 126') and the proportional ratio of the volume flows to one another are controlled in accordance with operating parameters which are detected by means of sensors, wherein the exhaust air volume flow and the air distribution in the working chamber (180) are controllable via the air inlet openings (124, 125, 126; 124', 125', 126').
摘要:
The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.
摘要:
Disclosed is a multi-stage thermal convection apparatus and uses thereof. In one embodiment, the invention features a three-stage thermal convection apparatus that includes a temperature shaping element for assisting a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device.
摘要:
The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides.
摘要:
A biological sample containment system (10) and a label (20) for a container that includes a first layer (22) having a first readable information portion (24) and a second layer (26) removably attached to the first layer (22), the second layer (26) having a second readable information portion (28), is disclosed. In one embodiment, the first layer (22) and the second layer (26) of the label (20) are both formed of a thermosensitive material. In one embodiment, at least a part of the first readable information portion (24) is identical to at least a part of the second readable information portion (28). The label (20) of the present disclosure allows a portion of the label (20) having readable information to be removed and adhered to a patient record, for example, while a portion of the label (20) having identical readable information remains on the container (12).