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889 条记录 (耗时 0.019 秒)

    METHOD FOR ASSAYING BIOLOGICAL SAMPLE COMPONENT
    115.
    发明公开
    METHOD FOR ASSAYING BIOLOGICAL SAMPLE COMPONENT 有权
    VERFAHREN ZUM AUSTESTEN BIOLOGISCHER PROBENKOMPONENTEN

    公开(公告)号：EP1158299A1

    公开(公告)日：2001-11-28

    申请号：EP00905409.9

    申请日：2000-02-29

    申请人： INTERNATIONAL REAGENTS CORPORATION

    发明人： KISHI, Koji, Research & Development Center, Int. ,  KAKUYAMA, Tsutomu, Res. & Development Center, Int. ,  OCHIAI, Koji, Research & Development Center, Int. ,  HASEGAWA, Yuzo, Research & Development Center, Int

    IPC分类号： G01N33/92 ,  C12Q1/44

    CPC分类号： C12Q1/60 ,  G01N33/92

    摘要： A method for quantitating a specific component in lipoproteins contained in a biological sample, for example, HDL (high-density lipoprotein), LDL (low-density lipoprotein) or VLDL (very low-density lipoprotein) by using a commonly employed automatic analyzer without centrifuging or making the reaction liquor cloudy due to complexes or aggregates. Namely, a controlling means, whereby an enzyme reaction can be carried out exclusively for the target component, is introduced into a method for enzymatically assaying a component in a specific lipoprotein fraction in the serum, thereby specifically assaying the component.

    摘要翻译： 通过使用通常使用的自动分析仪来定量生物样品中所含的脂蛋白中的特定成分的方法，例如HDL（高密度脂蛋白），LDL（低密度脂蛋白）或VLDL（极低密度脂蛋白））， 离心或使反应液由于复合物或聚集体而变得混浊。 也就是说，将针对目标成分进行酶反应的控制手段导入用于酶法测定血清中特定脂蛋白部分中的成分的方法，由此特异性地测定该成分。

    METHYLATED CpG ISLAND AMPLIFICATION (MCA)
    117.
    发明公开
    METHYLATED CpG ISLAND AMPLIFICATION (MCA) 审中-公开
    甲基化CpG岛扩增

    公开(公告)号：EP1127157A1

    公开(公告)日：2001-08-29

    申请号：EP99971462.9

    申请日：1999-11-02

    申请人： THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE

    发明人： Issa, Jean-Pierre ,  Baylin, Stephen ,  Toyota, Minoru

    IPC分类号： C12Q1/44 ,  C12Q1/48 ,  C12Q1/68 ,  C12P19/34 ,  C12N9/22 ,  C12N15/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6809 ,  C12Q1/48 ,  C12Q1/6855 ,  C12Q2531/113 ,  C12Q2525/191 ,  C12Q2521/331

    摘要： A method is provided for identifying a methylated CpG containing nucleic acid including contacting a nucleic acid with a methylation sensitive restriction endonuclease that cleaves unmethylated CpG sites, contacting the sample with an isoschizomer of the methylation sensitive restriction endonuclease, wherein the isoschizomer cleaves both methylated and unmethylated CpG sites. The method also includes adding an oligonucleotide under conditions and for a time to allow ligation of the oligonucleotide to nucleic acid cleaved by the restriction endonuclease, and amplifying the nucleic acid. A method is also provided for detecting an age associated disorder including contacting a nucleic acid with a methylation sensitive restriction endonuclease that cleaves unmethylated CpG sites, contacting the sample with an isoschizomer of the methylation sensitive restriction endonuclease, wherein the isoschizomer cleaves both methylated and unmethylated CpG sites. The method also includes adding an oligonucleotide under conditions and for a time to allow ligation of the oligonucleotide to nucleic acid cleaved by the restriction endonuclease, and amplifying the nucleic acid. The amplified digested nucleic acid is adhered to a membrane, and hybridized with a probe of interest. A kit useful for detection of a CpG containing nucleic acid is also provided.

    METHODS AND SYSTEMS FOR PREDICTING PROTEIN FUNCTION
    120.
    发明公开
    METHODS AND SYSTEMS FOR PREDICTING PROTEIN FUNCTION 审中-公开
    方法和系统，用于预测蛋白质的功能

    公开(公告)号：EP1108055A1

    公开(公告)日：2001-06-20

    申请号：EP99926014.4

    申请日：1999-05-27

    申请人： The Scripps Research Institute

    发明人： SKOLNICK, Jeffrey ,  FETROW, Jacquelyn, S.

    IPC分类号： C12Q1/00 ,  C12Q1/26 ,  C12Q1/37 ,  C12Q1/44 ,  C12N9/00 ,  C12N9/02 ,  C12N9/14 ,  C12N9/22 ,  C12N9/48 ,  C12N9/50 ,  G01N31/00 ,  G01N33/00 ,  A61K38/00 ,  C07K1/00

    CPC分类号： G06F19/18 ,  C07K2299/00 ,  G01N33/68 ,  G06F19/16 ,  G06F19/22

    摘要： The present invention concerns methods and systems for predicting the biological function(s) of proteins. The invention is based on the development of functional site descriptors for discrete protein biological functions. Functional site descriptors are geometric representations of protein functional sites in three-dimensional space, and can also include additional parameters, for example, conformational information. Following their development, one or more functional site descriptors (for one or more different biological funtions) are used to probe protein structures to determine if such structures contain the functional sites described by the corresponding functional site descriptors. If so, the protein(s) containing the functional site(s) are predicted to have the corresponding biological function(s). In preferred embodiments, a library of functional site descriptors is used to probe inexact protein structures derived by computational methods from amino acid sequence information to predict the biological function(s) of such sequences and of the gene(s) encoding the same.