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175 条记录 (耗时 0.019 秒)

    NUCLEIC ACID MARKER LADDER FOR ESTIMATING MASS
    21.
    发明公开
    NUCLEIC ACID MARKER LADDER FOR ESTIMATING MASS 失效
    核酸长度标准的评估质量

    公开(公告)号：EP0725821A1

    公开(公告)日：1996-08-14

    申请号：EP95901092.0

    申请日：1994-10-28

    申请人： LIFE TECHNOLOGIES, INC.

    发明人： HARTLEY, James, L.

    IPC分类号： C12N15 ,  C12Q1

    CPC分类号： C12Q1/68 ,  C12N15/10 ,  C12Q1/686

    摘要： The invention relates to a nucleic acid marker ladder which is a restriction endonuclease digest, wherein a nucleic acid restriction endonuclease digest is a collection of nucleic acid fragments resulting from complete digestion of one or more nucleic acids by one or more restriction endonucleases; the restriction endonuclease digest contains at least 3 fragments; and the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more.

    MODIFIED NUCLEOTIDES.
    24.
    发明公开
    MODIFIED NUCLEOTIDES. 失效
    修饰的核苷酸。

    公开(公告)号：EP0638087A4

    公开(公告)日：1994-12-27

    申请号：EP93907533

    申请日：1993-03-17

    申请人： LIFE TECHNOLOGIES INC

    发明人： HACES ALBERTO ,  FLICKINGER JEANNETTE L ,  MACKEY JESSE K ,  RASHTCHIAN AYOUB

    IPC分类号： C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C07H21/00 ,  C07H19/10 ,  C07H19/20

    摘要： The present invention provides novel modified nucleotide compounds having the general formula: X-n(J)-(d or r)NTP where N is adenosine, guanosine or cytidine; X is H, a fluorophore, a chromophore, a luminescent compound, a ligand or a hapten; n is an integer of 7 or more; and J is a Jeffamine (TM Texaco, Inc.) substituent. These compounds are substantially superior substrates for incorporating label into nucleic acids during enzyme-catalyzed synthesis. Use of a Jeffamine-linked labelled nucleotide derivative as a partial substitute for unlabelled substrate therefore leads to significantly greater incorporation, hence increased label density per unit length of nucleic acid, than the same label joined by a prior art linker. Jeffamine-modified ribo- or deoxyribo-nucleotides are also provided for synthesis of Jeffamine-modified nucleic acids. The invention further provides an improved method of synthesizing labelled nucleic acids. This method provides greater frequency and higher efficiency of label incorporation, thus requiring lower amounts of nucleotide derivative in the reaction mixture.

    Cloning and expressing restriction endonucleases and modification methylases from caryophanon
    25.
    发明公开
    Cloning and expressing restriction endonucleases and modification methylases from caryophanon 失效
    克罗尼昂和表达von Restriktionsendonukleasen und Modifikationsmethylasen von Karyophanon。

    公开(公告)号：EP0605854A2

    公开(公告)日：1994-07-13

    申请号：EP93120847.4

    申请日：1993-12-23

    申请人： LIFE TECHNOLOGIES, INC.

    发明人： Longo, Mary C. ,  Smith, Michael D. ,  Chatterjee, Deb K.

    IPC分类号： C12N15/55 ,  C12N9/22

    CPC分类号： C12N9/12 ,  C12N9/1007

    摘要： The present invention is directed to recombinant hosts which contain and express the Cla I Type-II restriction endonuclease and/or modification methylase genes. The present invention is also directed to vectors or DNA molecules which contain these gene, and to methods of producing the enzymes. One source of these enzyme is Caryophanon latum, although other microorganisms may be used to isolate the restriction endonuclease isoschizomers and modification methylase isoschizomers of the invention.

    摘要翻译： 重组宿主细胞 要求保护编码限制性内切核酸酶（I）的Caryophanon基因，其中（I）能够识别式（II）的回文DNA序列并将其切割在T和C残基之间以形成两碱基5'延伸。

    Process for controlling contamination of nucleic acid amplification reactions
    28.
    发明公开
    Process for controlling contamination of nucleic acid amplification reactions 失效
    Verfahren zur Beherrschung der Verunreinigung vonNukleinsäure-Amplifizierungsreaktionen。

    公开(公告)号：EP0522884A1

    公开(公告)日：1993-01-13

    申请号：EP92306396.0

    申请日：1992-07-13

    申请人： LIFE TECHNOLOGIES INC.

    发明人： Hartley, James L. ,  Berninger, Mark

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6848 ,  C12Q2531/137 ,  C12Q2525/101 ,  C12Q2521/531 ,  C12Q2525/121 ,  C12Q2521/319

    摘要： A method of incorporating an exo-sample nucleotide (e.g. deoxyuridine in DNA) into amplified product strands resulting from a nucleic acid amplification process is disclosed. Once the product strands have been obtained and analysed (e.g. by hybridisation, Southern blot, etc.), the exo-sample strands can be selectively destroyed by acting on the incorporated exo-sample nucleotide.
    Two procedures are described. In the first procedure the exo-sample nucleotide is incorporated by conducting the amplification reaction in the presence of an excess of exo-sample nucleotide triphosphate.
    In the second procedure the exo-sample nucleotide is incorporated by conducting the amplification reaction in the presence of an oligonucleotide which has, as part of its sequence, one or more exo-sample nucleotides.

    摘要翻译： 公开了将外切样品核苷酸（例如DNA中脱氧尿苷）掺入由核酸扩增过程产生的扩增产物链中的方法。 一旦获得并分析了产物链（例如通过杂交，Southern印迹等），可以通过作用于合并的外切核苷酸来选择性地破坏外部样品链。 描述两个步骤。 在第一个步骤中，通过在过量的外切核苷酸三磷酸的存在下进行扩增反应来引入外切核苷酸。 在第二程序中，通过在寡核苷酸的存在下进行扩增反应来引入外部样品核苷酸，所述寡核苷酸作为其序列的一部分，具有一个或多个外切核苷酸。

    CLONED $i(Kpn)I RESTRICTION-MODIFICATION SYSTEM
    29.
    发明公开
    CLONED $i(Kpn)I RESTRICTION-MODIFICATION SYSTEM 失效
    克隆的KpnI-限制修饰系统。

    公开(公告)号：EP0522018A1

    公开(公告)日：1993-01-13

    申请号：EP91907196.0

    申请日：1991-03-20

    申请人： LIFE TECHNOLOGIES INC.

    发明人： CHATTERJEE, Deb, Kumar ,  HAMMOND, Alan, Wayne

    IPC分类号： C12N1 ,  C12N9 ,  C12N15 ,  C12R1

    CPC分类号： C12N9/1007 ,  C12N9/22

    摘要： Clonage et expression dans un hôte tel que Escherichia coli du système de modification-restriction à partir de Klebsiella pneumoniae utilisant un protocole à deux étapes. La protection initiale de l'hôte E. coli avec une méthylase exprimée sur un vecteur a été nécessaire pour stabiliser un vecteur compatible portant à la fois les gènes d'endonucléase et de méthylase dans un seul fragment d'ADN. Un carte chromosique a été dressée pour localiser les gènes de méthylase et d'endonucléase de KpnI. On a construit une souche de E. coli qui produit des niveaux d'endonucléase KpnI plus élevés de plusieurs milliers de fois que le niveau produit par Klebsiella pneumoniae. La présente invention a aussi trait au clonage et à l'expression de gènes codant pour des isoschizomères d'endonucléase de restriction de KpnI et/ou des isoschizomères de méthylase de modification de la méthylase KpnI.