公开(公告)号：EP2373784B1

公开(公告)日：2017-10-25

申请号：EP09836895.4

申请日：2009-12-16

申请人： The Scripps Research Institute

发明人： LI, Wenlin ,  ZHOU, Hongyan ,  DING, Sheng

IPC分类号： C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2501/115 ,  C12N2501/16 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/727 ,  C12N2510/00

摘要： The present invention provides for the generation and maintenance of pluripotent cells by culturing the cells in the presence of an ALK5 inhibitor.

公开(公告)号：EP3231869A1

公开(公告)日：2017-10-18

申请号：EP17159873.3

申请日：2009-06-15

申请人： Whitehead Institute for Biomedical Research

发明人： JAENISCH, Rudolph ,  CAREY, Bryce Woodbury

IPC分类号： C12N15/12 ,  C12N15/867 ,  C12N5/10 ,  C12N5/074

CPC分类号： C12N15/86 ,  A01K67/0271 ,  C07K14/4705 ,  C12N5/0606 ,  C12N5/0696 ,  C12N15/79 ,  C12N15/85 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/11 ,  C12N2506/115 ,  C12N2510/00 ,  C12N2740/15041 ,  C12N2740/15043 ,  C12N2740/16043 ,  C12N2799/027 ,  C12N2800/108 ,  C12N2840/203 ,  G01N33/5008

摘要： The disclosure relates to a method of reprogramming one or more somatic cells, e.g., partially differentiated or fully/terminally differentiated somatic cells, to a less differentiated state, e.g., a pluripotent or multipotent state. In further embodiments the invention also relates to reprogrammed somatic cells produced by methods of the invention, to chimeric animals comprising reprogrammed somatic cells of the invention, to uses of said cells, and to methods for identifying agents useful for reprogramming somatic cells.

公开(公告)号：EP2665811B1

公开(公告)日：2017-10-18

申请号：EP12737028.6

申请日：2012-01-19

申请人： The Regents of the University of California

发明人： TLSTY, Thea D.

IPC分类号： C12N5/071

CPC分类号： C12N5/0678 ,  C12N5/0606 ,  C12N5/0607 ,  C12N5/0662 ,  C12N2506/095

摘要： Aspects of the present invention are drawn to compositions of somatic cells with innate potential for pluripotency (SCIPP). SCIPP have the capacity to differentiate into functional derivatives of each of the major germ layers (i.e., ectodermal, endodermal and mesodermal). Also provided are methods and kits for identifying and isolating the somatic cells from a subject as well as for employing SCIPP for research or therapeutic purposes.

公开(公告)号：EP2770051B1

公开(公告)日：2017-09-27

申请号：EP12842151.8

申请日：2012-10-22

申请人： ARKRAY, Inc.

发明人： KOTERA, Hidetoshi ,  TATSUMI, Kazuya ,  YOKOKAWA, Ryuji ,  TADA, Takashi ,  NAGATA, Shogo ,  OKITSU, Teru ,  OKONOGI, Atsuhito ,  NODA, Yuichiro ,  NAKANISHI, Naoyuki ,  MATSUMURA, Taku ,  OSUMI, Takashi

IPC分类号： C12N5/10 ,  C12N5/00 ,  C12N5/071 ,  C12N5/0735 ,  C12M3/06 ,  C12N5/074

CPC分类号： C12N5/0606 ,  C12M23/16 ,  C12N5/0696 ,  C12N2501/115 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2502/13 ,  C12N2521/00 ,  C12N2533/54

公开(公告)号：EP2821480B1

公开(公告)日：2017-08-16

申请号：EP14187130.1

申请日：2010-10-07

申请人： Osaka University ,  Kyoto University

发明人： Sekiguchi, Kiyotoshi ,  Futaki, Sugiko ,  Taniguchi, Yukimasa ,  Hayashi, Maria ,  Nakatsuji, Norio ,  Miyazaki, Takamichi ,  Kawase, Eihachiro ,  Suemori, Hirofumi

IPC分类号： C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2533/52

公开(公告)号：EP3194581A2

公开(公告)日：2017-07-26

申请号：EP15841324.5

申请日：2015-09-15

申请人： Children's Medical Center Corporation

发明人： ZHANG, Yi ,  MATOBA, Shogo

IPC分类号： C12N9/02 ,  C12N5/075 ,  A01K67/027

CPC分类号： C12N15/877 ,  A01K67/0273 ,  A01K2227/105 ,  C12N5/0604 ,  C12N5/0606 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2517/04 ,  C12Y201/01043

摘要： The present invention provides methods and compostions to improve the efficiency of somatic cell nuclear transfer (SCNT) and the consequent production of nuclear transfer ESC (ntESC) and transgenic cells and/or non-human animals. More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of donor somatic cells prevents efficient somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of SCNT by exogenous or overexpression of the demethylase Kdm4 family and/or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases Suv39h1 and/or Suv39h2.

摘要翻译： 本发明提供了用于提高体细胞核转移（SCNT）效率和随后产生核转移ESC（ntESC）和转基因细胞和/或非人动物的方法和组合物。 更具体而言，本发明涉及在供体体细胞的核遗传物质中重编程耐受性区域（RRR）中组蛋白H3-赖氨酸9（H3K9me3）的三甲基化阻止有效体细胞核重编程或SCNT。 本发明提供了在通过外源或过表达脱甲基酶Kdm4家族和/或通过抑制组蛋白甲基转移酶Suv39h1和/或Suv39h2抑制H3K9me3的甲基化来提高SCNT功效的方法中减少H3K9me3的方法和组合物。

公开(公告)号：EP3194572A1

公开(公告)日：2017-07-26

申请号：EP15759548.9

申请日：2015-07-30

申请人： Yeda Research and Development Co. Ltd.

发明人： HANNA, Yaqub ,  NOVERSHTERN, Noa ,  RAIS, Yoach

IPC分类号： C12N5/0735 ,  C12N5/074 ,  C12N15/00

CPC分类号： C12N5/0696 ,  C12N5/0037 ,  C12N5/0606 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/235 ,  C12N2501/415 ,  C12N2501/50 ,  C12N2501/60 ,  C12N2501/71 ,  C12N2501/72 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/999 ,  C12N2506/00

摘要： A culture medium is disclosed which comprises STAT3 activator, an ERK1/2 inhibitor and an Axin stabilizer, and optionally also a PKC inhibitor. Cell cultures comprising same and uses thereof are also disclosed.

摘要翻译： 公开了包含STAT3激活剂，ERK1 / 2抑制剂和Axin稳定剂以及任选还有PKC抑制剂的培养基。 还公开了包含其的细胞培养物及其用途。

公开(公告)号：EP2398897B1

公开(公告)日：2017-06-28

申请号：EP10705729.1

申请日：2010-02-22

申请人： Cellular Dynamics International, Inc.

发明人： DAIGH, Christine, A. ,  FUHRKEN, Peter ,  SALVAGIOTTO, Giorgia

IPC分类号： C12N5/0735 ,  C12N5/0789

CPC分类号： C12N5/0606 ,  C12N5/0647 ,  C12N5/069 ,  C12N2500/02 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/70 ,  C12N2506/02

公开(公告)号：EP3061825A4

公开(公告)日：2017-05-03

申请号：EP14855992

申请日：2014-10-24

申请人： FUNDACIÓN PÚBLICA ANDALUZA PROGRESO Y SALUD

发明人： REAL LUNA PEDRO JOSÉ ,  GARCÍA TOSCANO MIGUEL ,  MENÉNDEZ BUJAN PABLO ,  MARTÍN MOLINA FRANCISCO ,  NAVARRO MONTERO ÓSCAR ,  AYLLÓN CASES VERÓNICA ,  RAMOS MEJÍA VERÓNICA ,  BUENO UROZ CLARA ,  COBO PULIDO MARIÉN ,  ROMERO ESCOBAR TAMARA

IPC分类号： C12N15/12 ,  C12M1/00 ,  C12M3/00 ,  C12N5/0735 ,  C12N5/074 ,  C12N5/078 ,  C12N15/86

CPC分类号： C12N5/0644 ,  C12M21/08 ,  C12M23/44 ,  C12M47/04 ,  C12N5/0606 ,  C12N5/0696 ,  C12N15/86 ,  C12N2501/065 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2506/02 ,  C12N2506/11 ,  C12N2740/15043

公开(公告)号：EP1797172B1

公开(公告)日：2017-04-19

申请号：EP05806471.8

申请日：2005-09-08

申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION

发明人： THOMSON, James, A. ,  LUDWIG, Tenneille

IPC分类号： C12N5/0735

CPC分类号： C12N5/0606 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/40 ,  C12N2500/44 ,  C12N2500/46 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/415 ,  C12N2501/845 ,  C12N2501/999 ,  C12N2533/52 ,  C12N2533/54