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    THAUMATIN AUS TRANSGENER GERSTE
    31.
    发明公开
    THAUMATIN AUS TRANSGENER GERSTE 审中-公开
    索马甜OFF基因大麦

    公开(公告)号：EP1910412A2

    公开(公告)日：2008-04-16

    申请号：EP06762900.6

    申请日：2006-07-28

    申请人： Maltagen Forschung GmbH

    发明人： STAHL, Rainer ,  LÜHRS, Renate ,  DARGATZ, Harald

    IPC分类号： C07K14/43 ,  C12N15/82

    CPC分类号： C12N15/8242

    摘要： Transgenic, monocotyledonous plants (A) of the genera Triticum, Hordeum, Avena, Secale, Penisetum or Sorghum that contain thaumatin (I), are new.. Independent claims are also included for: (1) a vector containing a construct or expression cassette that includes at least one polynucleotide (II) that encodes (I), at least one endosperm-specific promoter (III) and optionally a signal sequence; (2) transformed plant cells, tissue and protoplasts that, after transformation, contain (II) integrated stably into the genome; (3) protein (Ia) obtained from (A) and having Seq. (28); (4) polynucleotide (IIa) that encodes (Ia) and has Seq. (29); (5) grain/seed of (A) containing at least 0.5, especially 3 or more, g (I)/kg; (6) malted or milled product, particularly powder or extract, containing (I) and prepared from (A); (7) instant powder comprising the product of (6); (8) animal or human food or feed containing the grain/seed of (5) or products of (6); and (9) dietetic or low-calorie foods, and taste intensifiers, that contain products of (8).

    OIL BODY PROTEINS AS CARRIERS OF HIGH VALUE PROTEINS
    34.
    发明授权
    OIL BODY PROTEINS AS CARRIERS OF HIGH VALUE PROTEINS 失效
    油体蛋白肽品质在植物CARRIER

    公开(公告)号：EP0871749B1

    公开(公告)日：2006-08-16

    申请号：EP95941561.3

    申请日：1995-12-21

    申请人： SemBioSys Genetics Inc.

    发明人： MOLONEY, Maurice

    IPC分类号： C12N15/82 ,  C12N15/29 ,  C12N15/57 ,  C12N15/25 ,  C12N15/15 ,  C12N15/62 ,  A01H5/00

    CPC分类号： C12N9/6483 ,  C07K14/545 ,  C07K14/61 ,  C07K14/8139 ,  C07K14/815 ,  C07K2319/00 ,  C12N9/6429 ,  C12N15/8234 ,  C12N15/8241 ,  C12N15/8242 ,  C12N15/8257 ,  C12Y304/21005 ,  C12Y304/23004

    摘要： The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oleosins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens. Finally, the persistent association of oil body proteins with the oil body can be further utilized to develop a biological means to create novel immobilized enzymes useful for bioconversion of substrates.

    MONOTERPENE SYNTHASES FROM GRAND FIR ($i(ABIES GRANDIS))
    37.
    发明公开
    MONOTERPENE SYNTHASES FROM GRAND FIR ($i(ABIES GRANDIS)) 失效
    FIR的单萜合酶（冷杉桉）

    公开(公告)号：EP1032257A4

    公开(公告)日：2005-03-16

    申请号：EP98935641

    申请日：1998-07-10

    申请人： UNIV WASHINGTON

    发明人： BOHLMANN JOERG ,  STEELE CHRISTOPHER L ,  CROTEAU RODNEY B

    IPC分类号： A23D9/00 ,  C11B1/00 ,  C12N9/88 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10 ,  A23D7/00 ,  A23K1/14 ,  C07H21/04 ,  C07K4/10 ,  C12N15/04 ,  C12N15/63

    CPC分类号： A23D9/00 ,  C11B1/00 ,  C12N9/88 ,  C12N15/8242 ,  C12N15/8243

    摘要： cDNAs encoding myrcene synthase, (-)-limonene synthase and (-)-pinene synthase from Grand fir (Abies grandis) haven been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID NO:1; SEQ ID NO:3 and SEQ ID NO:5) are provided which code for the expression of myrcene synthase (SEQ ID NO:2), (-)-pinene synthase (SEQ ID NO:4) and (-)-limonene synthase (SEQ ID NO:6) , respectively, from Grand fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for myrcene synthase, (-)-limonene synthase and (-)-pinene synthase, or for a base sequence sufficiently complementary to at least a portion of myrcene synthase, (-)-limonene synthase or (-)-pinene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding myrcene synthase, (-)-limonene synthase or (-)-pinene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant myrcene synthase, (-)-limonene synthase or (-)-pinene synthase may be used to obtain expression or enhanced expression of myrcene synthase, (-)-limonene synthase or (-)-pinene synthase in plants in order to enhance the production of monoterpenoids, or may be otherwise employed for the regulation or expression of myrcene synthase, (-)-limonene synthase and (-)-pinene synthase, or the production of their products.