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49 条记录 (耗时 0.04 秒)

    PRODUCTION OF GLYCOPROTEINS WITH MAMMALIAN-LIKE N-GLYCANS IN FILAMENTOUS FUNGI
    47.
    发明公开
    PRODUCTION OF GLYCOPROTEINS WITH MAMMALIAN-LIKE N-GLYCANS IN FILAMENTOUS FUNGI 审中-公开
    哺乳动物丝状真菌中哺乳动物样N-糖苷生产糖蛋白

    公开(公告)号：EP3172333A1

    公开(公告)日：2017-05-31

    申请号：EP15738703.6

    申请日：2015-07-21

    申请人： Glykos Finland Oy

    发明人： NATUNEN, Jari ,  LEPPÄNEN, Anne ,  SALMINEN, Heidi ,  HILTUNEN, Jukka ,  KANERVA, Anne ,  HEISKANEN, Annamari ,  WESTERHOLM-PARVINEN, Ann ,  SCHMIDT, Georg ,  HUUSKONEN, Anne ,  MUSTALAHTI, Eero ,  LANDOWSKI, Christopher ,  SALOHEIMO, Markku ,  SAARINEN, Juhani ,  SOMMER, Benjamin Patrick ,  WAHL, Ramon ,  OSTERMEIER, Christian ,  HELK, Bernhard

    IPC分类号： C12P21/00 ,  C12N9/10 ,  C12N9/24

    摘要： The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such as Trichoderma cells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii.a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing α1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.

    摘要翻译： 本公开涉及用于在丝状真菌细胞例如木霉属细胞中产生重组糖蛋白的组合物和方法，其中所述产生的全部中性N-聚糖的至少90％（mol％），优选至少95％ 重组糖蛋白是哺乳动物样N-聚糖。 更具体地说，本发明提供了一种丝状真菌细胞，其包含i。 与不具有所述突变的亲本丝状真菌细胞相比，减少或消除一种或多种内源性蛋白酶活性的一种或多种突变; ii。编码寡糖基转移酶的异源催化亚基的多核苷酸; III。 用于增加α1,2甘露糖苷酶活性的重组多核苷酸;和iv。 编码所述异源糖蛋白的重组多核苷酸。

    CANCER SPECIFIC GLYCANS AND USE THEREOF
    49.
    发明公开
    CANCER SPECIFIC GLYCANS AND USE THEREOF 有权
    VERWENDUNGEN VON KREBSSPEZIFISCHEN GLYKANEN

    公开(公告)号：EP1910838A2

    公开(公告)日：2008-04-16

    申请号：EP06764481.5

    申请日：2006-07-20

    申请人： Glykos Finland Oy

    发明人： SATOMAA, Tero ,  NATUNEN, Jari ,  HEISKANEN, Annamari ,  OLONEN, Anne ,  SAARINEN, Juhani ,  SALOVUORI, Noora ,  HELIN, Jari

    IPC分类号： G01N33/574

    CPC分类号： C07K16/44 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/76 ,  C12Q1/34 ,  G01N33/574 ,  G01N33/57488 ,  G01N33/57492 ,  G01N2400/38 ,  Y10T436/143333

    摘要： The present invention describes glycans, which are specifically expressed by certain cancer cells, tumours and other malignant tissues. The present invention describes methods to detect cancer specific glycans as well as methods for the production of reagents binding to said glycans. The invention is also directed to the use of said glycans and reagents binding to them for the diagnostics of cancer and malignancies. Furthermore, the invention is directed to the use of said glycans and reagents binding to them for the treatment of cancer and malignancies. Moreover, the present invention comprises efficient methods to differentiate between malignant and benign tumors by analyzing glycan structures.

    摘要翻译： 本发明描述了由某些癌细胞，肿瘤和其它恶性组织特异性表达的聚糖。 本发明描述了检测癌症特异性聚糖的方法以及生产结合所述聚糖的试剂的方法。 本发明还涉及所述聚糖和与其结合的试剂用于诊断癌症和恶性肿瘤的用途。 此外，本发明涉及所述聚糖和与其结合的试剂用于治疗癌症和恶性肿瘤的用途。 此外，本发明包括通过分析聚糖结构来区分恶性和良性肿瘤的有效方法。