公开(公告)号：EP3393575A1

公开(公告)日：2018-10-31

申请号：EP16880117.3

申请日：2016-12-22

申请人： Inovio Pharmaceuticals, Inc.

发明人： STADELMANN, Beat ,  KEMMERRER, Stephen

IPC分类号： A61N1/30 ,  A61N1/00 ,  A61N1/02 ,  A61N1/04 ,  C12N13/00

CPC分类号： A61N1/0412 ,  A61N1/303

摘要： An electroporation device having a battery pack including a plurality of battery cells and at least one lead in electrical communication with a circuit board. The battery pack including a safety switch and a controller for selectively placing the battery cells in electrical communication with the one lead. Where the controller is in operable communication with the safety switch such that when the controller detects one or more operating conditions the controller instructs the safety switch to electrically isolate the lead from the battery cells. The battery pack also includes a manual switch, and where activation of the switch causes the controller to instruct the safety switch to electrically isolate the lead from the battery cells.

公开(公告)号：EP3019593B1

公开(公告)日：2018-10-17

申请号：EP14747100.7

申请日：2014-07-15

申请人： Fermentalg

发明人： GARNIER, Patrice ,  PAGLIARDINI, Julien ,  CALLEJA, Pierre

IPC分类号： C12N1/12 ,  C12N1/14 ,  C12N1/20 ,  C12N13/00 ,  C12P7/64 ,  C12P23/00

CPC分类号： C12P23/00 ,  A01G33/00 ,  C12N1/12 ,  C12N1/14 ,  C12N1/20 ,  C12N13/00 ,  C12P7/6427 ,  C12P7/6472

公开(公告)号：EP3363894A1

公开(公告)日：2018-08-22

申请号：EP18160689.8

申请日：2011-08-04

申请人： MERCK SHARP & DOHME CORP.

发明人： Krah, David L. ,  Dehaven, Jill ,  Kriss, Jennifer A. ,  Barr, Colleen M. ,  Yagodich, Mary

IPC分类号： C12N7/04 ,  C12N13/00 ,  A61K39/25 ,  A61K39/00

CPC分类号： A61K39/25 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/54 ,  A61K2039/55 ,  C12N7/00 ,  C12N7/04 ,  C12N13/00 ,  C12N2710/16734 ,  C12N2710/16761

摘要： The invention provides an inactivated varicella zoster virus (VZV), and compositions and vaccines comprising said inactivated VZV, wherein the infectivity of the VZV is undetectable and wherein the inactivated VZV induces an immune response against VZV when administered to a patient. In embodiments of the compositions described herein, the VZV is inactivated with gamma radiation. The invention also provides a method of preparing an inactivated VZV vaccine, the method comprising gamma irradiating a sample comprising a VZV using from about 5 kGy to about 50 kGy of gamma radiation. Also provided by the invention herein is a method of treatment of or immunization against HZ or other disease associated with the reactivation of VZV, the method comprising administering to a subject a vaccine or pharmaceutical composition comprising a therapeutically effective amount of an inactivated VZV and a pharmaceutically acceptable carrier, wherein the VZV is inactivated by gamma irradiation.

公开(公告)号：EP2940120B1

公开(公告)日：2018-08-01

申请号：EP14166918.4

申请日：2014-05-02

申请人： Lonza Cologne GmbH

发明人： Altrogge, Ludger ,  Gleissner, Timo ,  Heinze, Andreas ,  Hermsmeier, Sven

IPC分类号： A61N1/04 ,  C12M1/42 ,  C12N13/00 ,  C12N15/87

CPC分类号： C12N13/00 ,  A61N1/0412 ,  C12M35/02 ,  C12N15/87

摘要： The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space 40 for holding the suspension, the internal space 40 comprising at least two segments 41, 42, wherein each segment 41, 42 comprises at least one electrode 43, 44 and wherein neighboring electrodes 43, 44 are separated from each other by at least one gap 47 which is at least partially filled with an insulating material 46, and wherein the edges of the electrodes 43, 44 facing each other within the internal space 40 are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method, wherein voltage is applied to at least one active electrode 43, 44 while the electrodes 43, 44, 45 or electrode segments next and/or opposite to the active electrode 43, 44 are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the electrically active area is locally limited and the field lines are focused near the active electrode and thus control of the process is enhanced.

公开(公告)号：EP3350324A1

公开(公告)日：2018-07-25

申请号：EP16846943.5

申请日：2016-09-13

申请人： Wroclawskie Centrum Badan EIT + SP Z O.O.

发明人： LIPINSKI, Tomasz ,  SKOWICKI, Michal ,  BEDNARKIEWICZ, Artur

IPC分类号： C12N13/00 ,  G01N21/64

CPC分类号： G01N21/6428 ,  C07K16/00 ,  C07K2317/14 ,  G01N21/6458 ,  G01N21/6489 ,  G01N2021/6441

摘要： The object of the invention is the method of detection and selection of hybridoma cells capable to produce desired antibodies, comprising seeding the hybridoma cells in a culture vessel with biofunctionalized surface, containing culture medium, adding biofunctionalized luminescent labels and incubating such hybridoma cell culture, followed by optical detection of hybridoma cells producing desired antibodies by reaction of he biofunctionalized luminescent labels with the antibodies and detecting a luminescent label's signal border around hybridoma cells producing desired antibodies, and further separation in situ hybridoma cells producing the given antibody type from the rest of the cells.

公开(公告)号：EP3344746A1

公开(公告)日：2018-07-11

申请号：EP16843109.6

申请日：2016-09-02

申请人： Rutgers, The State University of New Jersey

发明人： ZAHN, Jeffrey ,  ZHENG, Mingde ,  SHREIBER, David, I. ,  LIN, Hao ,  SHAN, Jerry, W.

IPC分类号： C12M1/42 ,  C12N13/00 ,  G01N15/08 ,  G01N27/02 ,  G01N33/48

CPC分类号： C12M1/42 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0645 ,  C12M23/16 ,  C12M35/02 ,  C12N13/00 ,  G01N15/1031 ,  G01N15/1056 ,  G01N2015/1006

摘要： Systems and methods for cell electroporation and molecular delivery using an intelligent, feedback controlled, microscale electroporation system for transfecting single cells.

公开(公告)号：EP3272854A1

公开(公告)日：2018-01-24

申请号：EP16180502.3

申请日：2016-07-21

申请人： Endonovo Therapeutics, Inc.

发明人： RUDD, Donnie

IPC分类号： C12N5/00 ,  C12N13/00

CPC分类号： C12N13/00 ,  C12N5/0062 ,  C12N2529/00

摘要： The disclosure describes a method for producing native mammalian biological molecules by electromagnetically stimulating mammalian cells using an electromagnetic field. The mammalian cells can be grown in a chamber that is exposed to a time varying electromagnetic force. The biological molecules can be isolated from the cells.

摘要翻译： 本公开描述了使用电磁场通过电磁刺激哺乳动物细胞来产生天然哺乳动物生物分子的方法。 哺乳动物细胞可以在暴露于随时间变化的电磁力的腔室中生长。 生物分子可以从细胞中分离出来。

公开(公告)号：EP3269803A2

公开(公告)日：2018-01-17

申请号：EP17188092.5

申请日：2010-11-09

申请人： Q Therapeutics, Inc.

发明人： SANDROCK, Robert ,  CAMPANELLI, James T. ,  EPPSTEIN, Deborah A.

IPC分类号： C12N5/071 ,  C12N13/00

CPC分类号： C12N5/0622

摘要： Methods for producing a population of human-derived glial restricted progenitor cells (GRPs) with decreased potentially unintended or undesired cellular phenotypes and/or decreased standard deviation in the cells of the population are provided. Also provided are antibody panels and gene expression profiles to characterize GRPs and a method for its use in characterizing GRP cells. In addition methods for use of these GRP cells to generate astrocytes and/or oligodendrocytes, to re-myelinate neurons and to treat glial cell related and other neurodegenerative diseases or disorders or injuries or damage to the nervous system are provided. A method to manufacture neural cells depleted of A2B5 positive cells is also provided.

摘要翻译： 提供了用于产生具有降低的潜在的非预期或不希望的细胞表型和/或群体细胞中标准偏差减少的人源神经胶质限制性祖细胞（GRP）的方法。 还提供了用于表征GRP的抗体组和基因表达谱以及其用于表征GRP细胞的方法。 此外，还提供了使用这些GRP细胞产生星形胶质细胞和/或少突胶质细胞，重新髓鞘化神经元和治疗神经胶质细胞相关的和其他神经退行性疾病或障碍或神经系统损伤或损伤的方法。 还提供了制造贫化A2B5阳性细胞的神经细胞的方法。

公开(公告)号：EP3137618A4

公开(公告)日：2017-12-13

申请号：EP15785253

申请日：2015-04-27

申请人： EPIMERON INC

发明人： FACCHINI PETER JAMES

IPC分类号： C12N13/00 ,  C12N15/00 ,  C12N15/10 ,  C12N15/52 ,  C12N15/54 ,  C12N15/62 ,  C12N15/63 ,  C12P13/00 ,  C12P17/00 ,  C12P17/12 ,  C12Q1/68 ,  C40B10/00 ,  C40B20/00 ,  C40B30/00

CPC分类号： C12P17/12 ,  C12N15/1082 ,  C12N15/1096 ,  C12N15/63 ,  C12Y201/01 ,  C40B10/00 ,  C40B20/00

摘要： Novel methods that may be used for the manufacture of plant alkaloid compounds and novel polynucleotide compounds are provided. The plant alkaloid compounds are useful as medicinal compounds.

公开(公告)号：EP3250704A1

公开(公告)日：2017-12-06

申请号：EP16705227.3

申请日：2016-01-25

申请人： Roquette Frères

发明人： PATINIER, Samuel

IPC分类号： C12P21/00 ,  C12N1/02 ,  C12N1/06 ,  C12N1/12 ,  C12N13/00

CPC分类号： A23J1/009 ,  A23J3/347 ,  A23L27/235 ,  A23L27/88 ,  A23L33/18 ,  C07K1/36 ,  C07K14/405 ,  C12N1/02 ,  C12N1/066 ,  C12N1/12 ,  C12P1/00 ,  C12P21/00

摘要： The invention relates to a method for fractionating components of a biomass of protein-rich microalgae of the
Chlorella genus, characterised in that it comprises the following steps: providing a biomass of microalgae produced by fermentation; optionally, washing the biomass to remove the interstitial soluble compounds; thermally permeabilising the biomass at a temperature of 0ºC to 150°C, preferably 100ºC to 150°C, for a time of several seconds to 5 minutes, preferably for a time of 5 seconds to 1 minute; separating the biomass thus permeabilised from the soluble fraction using a centrifugation technique, specifically multi-stage centrifugation; optionally, recovering and clarifying the soluble fraction thus obtained by microfiltration so as to remove residual insolubles from same; separating the aforementioned soluble fraction by precipitation, in order to obtain a peptidic isolate and a peptidic concentrate.