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    HIGH MOLECULAR WEIGHT RECOMBINANT SILK OR SILK-LIKE PROTEIN, AND MICRO- OR NANOSCALE SPIDER-WEB FIBER OR SPIDER-WEB-LIKE FIBER PRODUCED USING THE RECOMBINANT SILK OR SILK-LIKE PROTEIN
    51.
    发明公开
    HIGH MOLECULAR WEIGHT RECOMBINANT SILK OR SILK-LIKE PROTEIN, AND MICRO- OR NANOSCALE SPIDER-WEB FIBER OR SPIDER-WEB-LIKE FIBER PRODUCED USING THE RECOMBINANT SILK OR SILK-LIKE PROTEIN 审中-公开
    重组丝蛋白或组织样蛋白由高分子量，并从重组丝蛋白或像蛋白生产或超细纤维纳米级蜘蛛网和蜘蛛网状纤维

    公开(公告)号：EP2546263A2

    公开(公告)日：2013-01-16

    申请号：EP11713459.3

    申请日：2011-03-11

    Applicant: Korea Advanced Institute of Science and Technology

    Inventor： LEE, Sang Yup ,  XIA, Xiaoxia ,  QIAN, Zhi Gang ,  LEE, Jeong Wook ,  PARK, Young Hwan

    IPC: C07K14/435 ,  C12N15/12 ,  C12N15/63 ,  D01F4/00

    CPC classification number: C07K14/43518 ,  D01D5/0007 ,  D01D5/06 ,  D01F4/02

    Abstract: The present invention relates to a high-molecular-weight recombinant silk or silk-like protein having a molecular weight which is substantially similar to that of native silk protein, and to a micro- or nano-sized spider silk or silk-like fiber having improved physical properties, produced therefrom. The recombinant silk or silk-like protein according to the invention has high molecular weight, like dragline silk proteins from spiders, while a fiber produced therefrom has excellent physical properties compared to a fiber produced from native silk protein. Thus, the recombinant silk or silk-like protein and the spider silk or silk-like fiber produced therefrom will be highly useful in various industrial applications, including bioengineering applications and medical applications.

    Abstract translation: 本发明涉及一种高分子量的重组丝或丝样蛋白质基本上所有的分子量，其类似于做天然丝蛋白的，并以微米或纳米尺寸的蜘蛛丝或丝状纤维具有 改进的物理性能，从那里产生的。 重组丝或丝样蛋白gemäß到，本发明具有高的分子量，例如从蜘蛛拖丝蛋白质，而纤维产生有从具有优异的物理性能相比于从天然丝蛋白生产的纤维。 因此，重组丝或丝样蛋白和蜘蛛丝或由其产生丝状纤维将是在各种工业应用中，包括生物工程应用和医疗应用中非常有用的。

    PREPARATION METHOD OF LACTATE POLYMERS AND LACTATE COPOLYMERS USING POLYHYDROXYALKANOATE SYNTHASE MUTANTS
    53.
    发明公开
    PREPARATION METHOD OF LACTATE POLYMERS AND LACTATE COPOLYMERS USING POLYHYDROXYALKANOATE SYNTHASE MUTANTS 审中-公开
    聚羟基乙酸酯合成酶突变体制备乳酸聚合物和乳酸盐共聚物的方法

    公开(公告)号：EP2471910A2

    公开(公告)日：2012-07-04

    申请号：EP10794355.7

    申请日：2010-06-30

    Applicant: LG Chem, Ltd. ,  Korea Advanced Institute of Science and Technology

    Inventor： YANG, Taek Ho ,  KANG, Hye Ok ,  LEE, Sang Yup ,  JUNG, Yu Kyung

    IPC: C12N9/00 ,  C12N15/52 ,  C12P7/56

    CPC classification number: C12P7/625 ,  C12N9/13 ,  C12P7/56 ,  C12Y208/03001

    Abstract: Mutants of various polyhydroxyalkanoate (PHA) synthases capable of synthesizing a lactate polymer (PLA) and a lactate copolymer (PLA copolymer), and a method of preparing a lactate polymer and a lactate copolymer using the same are provided. More specifically, a mutant of polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8, and a method of preparing lactate polymer and lactate copolymer using the mutant of synthase are provided. The polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8 can have an activity of synthesizing a lactate polymer and a lactate copolymer by an amino acid sequence mutation affecting an activity of synthesizing a lactate polymer, and can produce a lactate polymer and a copolymer that have different features, respectively, by using the mutants of the synthase.

    Abstract translation: 提供了能够合成乳酸酯聚合物（PLA）和乳酸酯共聚物（PLA共聚物）的各种聚羟基链烷酸酯（PHA）合酶的突变体，以及使用该突变体制备乳酸酯聚合物和乳酸酯共聚物的方法。 更具体而言，提供了SEQ ID NO：2,4,6或8中所示的聚羟基链烷酸酯合酶的突变体，以及使用该合酶突变体来制备乳酸盐聚合物和乳酸盐共聚物的方法。 在SEQ ID NO：2,4,6或8中列出的聚羟基链烷酸酯合酶可以具有通过影响合成乳酸聚合物活性的氨基酸序列突变合成乳酸酯聚合物和乳酸酯共聚物的活性，并且可以产生 乳酸聚合物和具有不同特征的共聚物，分别通过使用合酶的突变体。

    MUTANT MICROORGANISM WITH HIGH ABILITY OF PRODUCING PUTRESCINE AND PREPARATION OF PUTRESCINE USING SAME
    54.
    发明公开
    MUTANT MICROORGANISM WITH HIGH ABILITY OF PRODUCING PUTRESCINE AND PREPARATION OF PUTRESCINE USING SAME 有权
    MUTIERTER MIKROORGANISMUS MIT HOHEM PUTRESCIN-PRODUKTIONSVERMÖGENUND HERSTELLUNG VON PUTRESCIN DAMIT

    公开(公告)号：EP2281880A2

    公开(公告)日：2011-02-09

    申请号：EP09729276.7

    申请日：2009-03-05

    Applicant: Korea Advanced Institute of Science and Technology

    Inventor： LEE, Sang Yup ,  QIAN, Zhi Gang ,  XIA, Xiaoxia ,  JEON, Yong Jae

    IPC: C12N15/00

    CPC classification number: C12P13/001

    Abstract: The present invention relates to mutant microorganisms having high ability to produce putrescine in which genes involved in the putrescine degradation or utilization pathway of microorganisms having a putrescine-producing metabolic pathway are inactivated or deleted; and a method for producing putrescine in high yield by culturing the mutant microorganisms under anaerobic conditions. The mutant microorganisms having high ability to produce putrescine are useful for producing a high yield of putrescine which is used in a wide range of industrial applications.

    Abstract translation: 本发明涉及产生腐胺的能力较强的突变微生物，其中涉及具有产生腐胺的代谢途径的微生物的腐胺降解或利用途径的基因被灭活或缺失; 以及通过在厌氧条件下培养突变体微生物，以高产率产生腐胺的方法。 具有高腐蚀能力的突变微生物对于产生广泛的工业应用的高产量的腐胺是有用的。

    METHOD FOR SCREENING ESSENTIAL METABOLITES IN GROWTH OF MICROORGANISMS
    56.
    发明公开
    METHOD FOR SCREENING ESSENTIAL METABOLITES IN GROWTH OF MICROORGANISMS 审中-公开
    法中生长的微生物的筛选挥发代谢物

    公开(公告)号：EP2097743A1

    公开(公告)日：2009-09-09

    申请号：EP07851674.7

    申请日：2007-12-21

    Applicant: Korea Advanced Institute of Science and Technology

    Inventor： LEE, Sang Yup ,  JEONG, Hawoong ,  KIM, Tae Yong ,  KIM, Pan-Jun ,  LEE, Kwang Ho

    IPC: G01N33/48

    CPC classification number: C12Q1/18 ,  G01N33/5038

    Abstract: The present invention disclosed is a method for screening metabolites essential for the growth of microorganism using metabolic flux analysis. More specifically, the present invention relates to the method for screening metabolites essential for the growth of microorganism, by selecting a target microorganism, constructing a metabolic network model of the selected microorganism, inactivating the consumption reaction of each of metabolites in the constructed metabolic network model, analyzing the metabolic flux of the metabolites to select metabolites essential for the growth of the microorganism, and confirming the selected metabolites using the utilization of each of the metabolites, defined as flux sum (Φ ). According to the present invention, metabolites essential for the growth of microorganism, and genes involved in the essential metabolites, can be screened in a convenient manner, and drug-target genes against pathogenic microorganisms can be predicted by deleting genes associated with the metabolites screened according to the method.

    METHOD OF IN-SILICO IMPROVEMENT OF ORGANISMS USING THE FLUX SUM OF METABOLITES
    57.
    发明公开
    METHOD OF IN-SILICO IMPROVEMENT OF ORGANISMS USING THE FLUX SUM OF METABOLITES 审中-公开
    程序对生物体的IN-硅片使用改进代谢物与总流量

    公开(公告)号：EP1915459A1

    公开(公告)日：2008-04-30

    申请号：EP05804474.4

    申请日：2005-10-14

    Applicant: Korea Advanced Institute of Science and Technology

    Inventor： LEE, Sang Yup ,  KIM, Tae Yong ,  LEE, Dong Yup

    IPC: C12Q1/68

    CPC classification number: G06F19/12 ,  G06F19/18

    Abstract: The present invention relates to an in silico method for improving an organism on the basis of the flux sum (Φ) of metabolites, and more particularly to a method for screening key metabolites that increase the production yield of a useful substance, the method comprising defining the metabolite utilization of an organism for producing a useful substance as flux sum and perturbing the flux sum, as well as a method for improving an organism producing a useful substance, the method comprising deleting and/or amplifying genes associated with the aforementioned screened key metabolites. According to the present invention, the correlation between specific metabolites and useful substance production can be exactly predicted, so that it is possible to develop an organism having increased useful substance production by introducing and/or amplifying and/or deleting genes expressing enzymes associated with the specific metabolites. In addition, it is also possible to increase the production of a useful substance by adding specific metabolites during culture.

    NOVEL RUMEN BACTERIA VARIANTS AND PROCESS FOR PREPARING SUCCINIC ACID EMPLOYING THE SAME
    58.
    发明公开
    NOVEL RUMEN BACTERIA VARIANTS AND PROCESS FOR PREPARING SUCCINIC ACID EMPLOYING THE SAME 有权
    NEW几乎阿根细菌变异体和工艺琥珀酸及其制备使用

    公开(公告)号：EP1692271A1

    公开(公告)日：2006-08-23

    申请号：EP04734158.1

    申请日：2004-05-20

    Applicant: Korea Advanced Institute of Science and Technology

    Inventor： LEE, Sang Yup ,  LEE, Sang Jun

    IPC: C12N1/21

    CPC classification number: C12P7/46 ,  C12N1/20 ,  C12R1/01

    Abstract: The present invention relates to novel rumen bacterial mutants resulted from the disruption of a lactate dehydrogenase gene (ldhA) and a pyruvate formate-lyase gene (pfl) (which are involved in the production of lactic acid, formic acid and acetic acid) from rumen bacteria; a novel bacterial mutant (Mannheimia sp. LPK7) having disruptions of a lactate dehydrogenase gene (ldhA), a pyruvate formate-lyase gene (plf), a phosphotransacetylase gene (pta), and a acetate kinase gene (ackA); a novel bacterial mutant (Mannheimia sp. LPK4) having disruptions of a lactate dehydrogenase gene (ldhA), a pyruvate formate-lyase gene (pfl) and a phosphoenolpyruvate carboxylase gene (ppc) involved in the immobilization of CO2 in a metabolic pathway of producing succinic acid; and a method for producing succinic acid, which is characterized by the culture of the above mutants in anaerobic conditions. The inventive bacterial mutants have the property of producing succinic acid at high concentration while producing little or no organic acids, as compared to the prior wild-type strains of producing various organic acids. Thus, the inventive bacterial mutants are useful as strains for the industrial production of succinic acid.

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