公开(公告)号：EP2290085A2

公开(公告)日：2011-03-02

申请号：EP10178355.3

申请日：2004-01-15

申请人： Danisco A/S

发明人： de Kreij, Arno ,  Madrid, Susan Mampusti ,  Mikkelsen, Jørn Dalgaard ,  Søe, Jørn Borch

IPC分类号： C12N15/54 ,  C12N15/55 ,  C12N9/10 ,  C12N9/18 ,  C12N11/00 ,  C12P7/62 ,  C12P7/64 ,  A23L1/03 ,  A23L1/035 ,  C12Q1/44 ,  C12Q1/48

CPC分类号： A21D8/042 ,  A23C19/0328 ,  A23D7/013 ,  A23G9/363 ,  A23L15/25 ,  A23L27/60 ,  A23L29/06 ,  A23L29/10 ,  A23L33/11 ,  C11C3/10 ,  C12N9/1029 ,  C12P7/62 ,  C12P7/6436 ,  C12P7/6454 ,  C12P7/6481 ,  C12Q1/48 ,  C12Y203/01 ,  C12Y301/01 ,  C12Y301/01003 ,  C12Y301/01026 ,  C12Y301/01032 ,  G01N2333/91051 ,  G01N2333/918

摘要： A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an additional emulsifier.

摘要翻译： 一种在食品中原位生产乳化剂的方法，其中将脂质酰基转移酶加入到食品中。 优选地，在不增加或不显着增加食品的游离脂肪酸含量的情况下生产乳化剂。 优选地，脂质酰基转移酶是能够将酰基从脂质转移到以下酰基受体中的一种或多种：甾醇，甾烷醇，碳水化合物，蛋白质或其亚单位的甘油。 优选地，除了乳化剂之外，可以制备一种或多种甾烷醇酯或甾烷醇酯或蛋白质酯或碳水化合物酯或甘油二酯或甘油单酯。 这些中的一种或多种可以用作另外的乳化剂。

公开(公告)号：EP1287154A4

公开(公告)日：2010-12-15

申请号：EP01946213

申请日：2001-06-07

申请人： PACIFIC BIOSCIENCES CALIFORNIA

发明人： WILLIAMS JOHN G K ,  BASHFORD GREGORY R ,  CHEN JIYAN ,  DRANEY DAN ,  NARAYANAN NARA ,  REYNOLDS BAMBI L ,  SHEAFF PAMELA

IPC分类号： C07H19/10 ,  G01N21/64 ,  C07B61/00 ,  C07H19/20 ,  C07H21/00 ,  C12N15/09 ,  C12Q1/00 ,  C12Q1/42 ,  C12Q1/44 ,  C12Q1/48 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/483 ,  G01N33/53

CPC分类号： C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/6869 ,  C40B40/00 ,  C12Q2565/629 ,  C12Q2565/607 ,  C12Q2565/301

公开(公告)号：EP2194142A1

公开(公告)日：2010-06-09

申请号：EP10002306.8

申请日：2003-11-26

申请人： DAIICHI PURE CHEMICALS CO., LTD.

发明人： Yamamoto, Shoko ,  Yamamoto, Mitsuaki ,  Nakanishi, Kazuo ,  Saito, Kazunori

IPC分类号： C12Q1/60 ,  C12Q1/00 ,  G01N33/92 ,  C12Q1/26 ,  C12Q1/44

CPC分类号： G01N33/92 ,  G01N2800/044 ,  Y10T436/25125

摘要： A method of measuring a cholesterol in high density lipoproteins characterized by using a polycyclic polyoxyalkylene derivative at least in the step of determining the specificity of the measurement of the target lipid.

摘要翻译： 至少在确定目标脂质测定的特异性的步骤中，测定高密度脂蛋白中的胆固醇的方法，其特征在于使用多环聚氧亚烷基衍生物。

公开(公告)号：EP2105508B1

公开(公告)日：2010-06-09

申请号：EP09004297.9

申请日：2009-03-25

申请人： FUJIFILM Corporation

发明人： Arakawa, Jun ,  Masuda, Nobuhito ,  Nakamura, Kentaro ,  Kageyama, Shigeki

IPC分类号： C12Q1/44 ,  G01N33/52

CPC分类号： C12Q1/44 ,  G01N2333/92

公开(公告)号：EP2157190A1

公开(公告)日：2010-02-24

申请号：EP08752790.9

申请日：2008-05-15

申请人： Ajinomoto Co., Inc.

发明人： FUKUCHI, Naoyuki

IPC分类号： C12Q1/48 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/44 ,  G01N33/15 ,  G01N33/50

CPC分类号： G01N33/573 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/44 ,  C12Q1/48 ,  G01N33/5432 ,  G01N2500/00

摘要： It is intended to provide an assay method whereby the activity of a lipid-modifying enzyme can be conveniently measured over a wide range and a drug capable of controlling a lipid-modifying enzyme with the use of this assay method. The above problem can be solved by, for example, a method of measuring the activity of a lipid-modifying enzyme which comprises the steps of (I) preparing a lipid micelle containing a biotinylated lipid and a substrate for the lipid-modifying enzyme; (II) bringing the lipid micelle prepared in the above step (I) into contact with the lipid-modifying enzyme; and (III) evaluating the activity of the lipid-modifying enzyme by applying an evaluation method using the proximity effect to the product obtained in the above step (II).

公开(公告)号：EP1534697A4

公开(公告)日：2009-12-16

申请号：EP03734195

申请日：2003-05-23

申请人： GIRI BRIJ P

发明人： GIRI BRIJ P

IPC分类号： C08F8/40 ,  C08F8/44 ,  C09K9/02 ,  C09K11/06 ,  C12Q1/00 ,  C12Q1/28 ,  G01N33/58 ,  C07D303/00 ,  C07F9/06 ,  C09K3/00 ,  C12Q1/44 ,  G01N21/76 ,  G01N33/53

CPC分类号： C12Q1/28 ,  C08F8/40 ,  C08F8/44 ,  C09K9/02 ,  C09K11/06 ,  C09K2211/1408 ,  C09K2211/1416 ,  C09K2211/1425 ,  C09K2211/1433 ,  C12Q2326/00 ,  G01N33/581 ,  G01N33/582 ,  G01N33/583

公开(公告)号：EP2116613A1

公开(公告)日：2009-11-11

申请号：EP08829199.2

申请日：2008-09-01

申请人： ARKRAY, Inc.

发明人： YAMAGUCHI, Takehiro ,  MIYAKE, Yuka

IPC分类号： C12Q1/60 ,  C12M1/34 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/44

CPC分类号： G01N33/92 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/44 ,  C12Q1/60 ,  G01N33/523 ,  G01N2333/904 ,  G01N2333/92

摘要： An LDL cholesterol measurement method that can be used with a test piece is provided. The LDL cholesterol measurement method for measuring LDL cholesterol in a sample has (A) a step of providing a total-cholesterol measurement portion and a non-LDL cholesterol measurement portion for measuring non-LDL cholesterol, which is cholesterol other than LDL cholesterol; (B) a step of measuring the total cholesterol in the sample in the total-cholesterol measurement portion; (C) a step of measuring the non-LDL cholesterol in the sample in the non-LDL cholesterol measurement portion; and (D) a step of obtaining the LDL cholesterol level in the sample by subtracting the non-LDL cholesterol value measured in the step (C) from the total-cholesterol value measured in the step (B).

摘要翻译： 提供可与试验片一起使用的低密度脂蛋白胆固醇测定方法。 用于测定样品中的LDL胆固醇的低密度脂蛋白胆固醇测定方法具有（A）提供用于测定作为LDL胆固醇以外的胆固醇的非低密度脂蛋白胆固醇的总胆固醇测定部和非低密度脂蛋白胆固醇测定部的步骤; （B）测定总胆固醇测定部中的样品中的总胆固醇的步骤; （C）测定非LDL胆固醇测定部中的样品中的非LDL胆固醇的步骤; 和（D）通过从步骤（B）中测量的总胆固醇值中减去步骤（C）中测量的非LDL胆固醇值，获得样品中的LDL胆固醇水平的步骤。

公开(公告)号：EP2116612A1

公开(公告)日：2009-11-11

申请号：EP08720965.6

申请日：2008-02-28

申请人： DENKA SEIKEN Co., Ltd.

发明人： ITOH, Yasuki

IPC分类号： C12Q1/44 ,  C12Q1/26 ,  C12Q1/30 ,  C12Q1/60 ,  G01N33/92

CPC分类号： G01N33/92 ,  C12Q1/44 ,  C12Q1/60 ,  Y10T436/104165 ,  Y10T436/25 ,  Y10T436/25125

摘要： A reagent for fractional measurement of small, dense LDLs without pretreatment of a specimen, which is adaptable to a rapid and convenient autoanalyzer, is provided. A method for quantitatively determining cholesterol in small, dense LDLs in a sample is further provided, comprising: a first step of eliminating lipoproteins other than small, dense LDLs in a sample in the presence of cholesterol esterase and 0.05 g/L to 1.0 g/L of a surfactant that acts on the lipoproteins other than small, dense LDLs; and a second step of quantitatively determining cholesterol in small, dense LDLs that remain after the first step.

公开(公告)号：EP1288306B1

公开(公告)日：2009-10-21

申请号：EP01934540.4

申请日：2001-06-05

申请人： SYSMEX CORPORATION

发明人： KISHI, Koji ,  KAKUYAMA, Tsutomu ,  OCHIAI, Koji

IPC分类号： C12Q1/44 ,  G01N33/92

CPC分类号： C12Q1/44 ,  C12Q1/60 ,  G01N33/92 ,  Y10S435/975

摘要： A method of selectively assaying components (in particular, cholesterol) in very low-density lipoprotein (VLDL) which is one of serum lipoproteins. In this assay, an enzyme reaction of lipoprotein lipase (LPL) or cholesterol esterase (CE), which are enzymes reacting well with high-density lipoprotein (HDL) and VLDL, is carried out in the presence of calixalene or its salt optionally together with one or more substances selected from among albumin and basic amino acids.

公开(公告)号：EP1849876A4

公开(公告)日：2009-07-22

申请号：EP06713669

申请日：2006-02-14

申请人： KYOWA MEDEX CO LTD

发明人： MIYAUCHI KAZUHITO ,  FUJINAKA MAYUMI

IPC分类号： C12Q1/60 ,  C12Q1/26 ,  C12Q1/34 ,  C12Q1/44 ,  G01N21/78 ,  G01N33/92

CPC分类号： C12Q1/60 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/44 ,  G01N33/92

摘要： It is intended to provide a quantification method whereby cholesterol in remnant-like lipoproteins can be conveniently and highly sensitively quantified without resorting to a procedure of separating a sample and a reagent and a kit therefor. A method for quantifying remnant-like lipoprotein cholesterol in a sample characterized by comprising, in an aqueous medium containing the sample and in the presence of a combination of specific surfactants and an enzyme hydrolyzing a phospholipid, reacting the remnant-like lipoprotein cholesterol in the sample with cholesterol ester hydrolase and cholesterol oxidase or cholesterol dehydrogenase (coexisting with an oxidized coenzyme) and then quantifying the hydrogen peroxide or the reduced coenzyme thus formed.