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    Compositions and methods to detect legionella pneumophila nucleic acid
    71.
    发明公开
    Compositions and methods to detect legionella pneumophila nucleic acid 审中-公开
    Zusammensetzungen und Verfahren zum Nachweisen von Legionella-pneumophila-Nukleinsäure

    公开(公告)号：EP2400037A1

    公开(公告)日：2011-12-28

    申请号：EP11006678.4

    申请日：2006-10-17

    申请人： Gen-Probe Incorporated

    发明人： Bungo, Jennifer, J. ,  Hogan, James J. ,  Pollner, Reinhold B. ,  Hudspeth, Marie K. ,  Kaplan, Shanon K. ,  Marlowe, Elizabeth M.

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/689 ,  Y02A50/451

    摘要： A set of oligonucleotides for use in determining the presence of Legionella pneumophila in a sample, said set of oligonucleotides comprising: a first amplification oligonucleotide, wherein the base sequence of said first amplification oligonucleotide is selected from the group consisting of SEQ ID NO:87, the RNA equivalent of SEQ ID NO:87, and the full complement(s) thereof; a second amplification oligonucleotide, wherein the base sequence of said second amplification oligonucleotide is selected from the group consisting of SEQ ID NO:71, the RNA equivalent of SEQ ID NO:71, and the full complement(s) thereof, and, optionally, a 5' promoter sequence; and a detection probe comprising a target complementary region, wherein the base sequence of said target complementary region is selected from the group consisting of the target complementary region of SEQ ID NO:88, the DNA equivalent of the target complementary region of SEQ ID NO:88, and the full complement(s) thereof.

    摘要翻译： 一组用于测定样品中嗜肺军团杆菌存在的寡核苷酸，所述寡核苷酸组包含：第一扩增寡核苷酸，其中所述第一扩增寡核苷酸的碱基序列选自SEQ ID NO：87， SEQ ID NO：87的RNA等效物及其全部补体; 第二扩增寡核苷酸，其中所述第二扩增寡核苷酸的碱基序列选自SEQ ID NO：71，SEQ ID NO：71的RNA当量及其全补体，以及任选地， 5'启动子序列; 和包含靶互补区的检测探针，其中所述靶互补区的碱基序列选自SEQ ID NO：88的靶互补区，SEQ ID NO：88的靶互补区的DNA当量。 88及其全部补充。

    Tagged oligonucleotides and their use in nucleic acid amplification methods
    74.
    发明公开
    Tagged oligonucleotides and their use in nucleic acid amplification methods 有权
    在Nukleinsäureverstärkungsverfahren的Markierte Oligonukleotide und ihre Verwendung

    公开(公告)号：EP2345740A1

    公开(公告)日：2011-07-20

    申请号：EP10015034.1

    申请日：2007-06-06

    申请人： Gen-Probe Incorporated

    发明人： Becker, Michael M. ,  Livezey, Kristin W. ,  Lam, Wai-Chung

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6865 ,  C12Q2525/301 ,  C12Q2525/186 ,  C12Q2525/155 ,  C12Q2525/161

    摘要： A pre-amplification reaction mixture for selective amplification of one or more target nucleic acid sequences, said reaction mixture comprising: a tagged oligonucleotide comprising first and second regions, said first region comprising a target hybridizing sequence hybridized to a target region contained at a 3'-end of one or more target nucleic acid sequences present in said reaction mixture and said second region comprising a tag sequence situated 5' to said target hybridizing sequence; a first oligonucleotide comprising a hybridizing sequence which hybridizes to a 3'-end of the complement of one or more of said target nucleic acid sequences; and a second oligonucleotide comprising a hybridizing sequence which hybridizes to the complement of said tag sequence, wherein said reaction mixture is substantially free of an active form of said tagged oligonucleotide which is not hybridized to said target region contained in said one or more target nucleic acid sequences present in said reaction mixture, wherein said active form of said tagged oligonucleotide has an available target hybridizing sequence for hybridization to said target region present in a non-target nucleic acid added to said reaction mixture, and wherein said reaction mixture does not comprise a nucleic acid polymerase capable of extending any of said oligonucleotides in a template-dependent manner.

    摘要翻译： 用于选择性扩增一个或多个靶核酸序列的预扩增反应混合物，所述反应混合物包含：包含第一和第二区域的标记寡核苷酸，所述第一区域包含与包含在3'端的靶区域杂交的靶杂交序列， 存在于所述反应混合物中的一个或多个靶核酸序列，并且所述第二区包含位于所述靶杂交序列5'的标签序列; 第一寡核苷酸，其包含与一个或多个所述靶核酸序列的补体的3'-末端杂交的杂交序列; 以及第二寡核苷酸，其包含与所述标签序列的互补序列杂交的杂交序列，其中所述反应混合物基本上不含所述标记的寡核苷酸的活性形式，其与所述一种或多种靶核酸中包含的所述靶标区域不杂交 存在于所述反应混合物中的序列，其中所述标记的寡核苷酸的所述活性形式具有用于与存在于添加到所述反应混合物中的非靶核酸中的所述靶区杂交的可用靶杂交序列，并且其中所述反应混合物不包含 能够以模板依赖的方式扩展任何所述寡核苷酸的核酸聚合酶。

    Systems amd methods to perform assays for detecting or quantifing analytes within samples
    77.
    发明公开
    Systems amd methods to perform assays for detecting or quantifing analytes within samples 有权
    系统和用于分析物的样品中检测或定量进行测定的方法

    公开(公告)号：EP2322940A1

    公开(公告)日：2011-05-18

    申请号：EP10010799.4

    申请日：2006-03-10

    申请人： Gen-Probe Incorporated

    发明人： Carrick, James M. ,  Davis, Christopher Brandon ,  Heinz, Robert E. ,  Knight, Bryon J. ,  Lair, Gary D. ,  Li, Florence F. ,  Li, Haito ,  Light, James P. ,  Macioszek, Jerzy A. ,  Nguyen, Thanh N. ,  Scalese, Robert F.

    IPC分类号： G01N35/02 ,  C12Q1/68

    CPC分类号： G01N21/6428 ,  B01L7/52 ,  C12Q1/6813 ,  C12Q1/6851 ,  G01N21/6454 ,  G01N21/76 ,  G01N35/00693 ,  G01N35/0092 ,  G01N35/0098 ,  G01N35/0099 ,  G01N35/025 ,  G01N35/026 ,  G01N35/028 ,  G01N35/04 ,  G01N35/1002 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2035/00346 ,  G01N2035/00356 ,  G01N2035/00524 ,  G01N2035/0097 ,  G01N2035/0441 ,  G01N2035/0443 ,  G01N2035/0444 ,  G01N2035/0455 ,  G01N2035/0491 ,  G01N2035/103 ,  G01N2201/0415 ,  Y10S435/808 ,  Y10S435/809 ,  Y10T436/113332 ,  Y10T436/25 ,  C12Q2537/165

    摘要： A computer-implemented method for determining the amount of an analyte of interest in a sample, the method comprising (a) forming amplification products from an analyte of interest, (b) collecting signal data with a signal measuring device concurrently with the formation of amplification products, the collected signal data comprising periodic measurements of the level of a signal emitted by the sample, wherein the level of signal emitted by the sample corresponds to the amount of amplification products formed by the sample, (c) storing the collected signal data in computer-readable media; and (d) using a computer programmed with an algorithm, which, when executed by the computer, is configured to cause the computer to access the data stored in step (c) and to adjust the data collected in step (b) to remove background contributions to the measured levels associated with sources other than the presence of the analyte of interest, normalize the data adjusted in step (d)(1) by dividing the adjusted data by the maximum level measured, adjusted to remove background contributions, approximate a curve through a portion of the adjusted, normalized data between a predetermined low bound stored on computer-readable media and a predetermined high bound stored on computer-readable media, determine an emergence time at which the curve approximated in step (d)(3) crosses a predetermined threshold data level and determine the amount of the analyte of interest by comparing the emergence time determined in step (d)(4) with threshold times determined for known amounts of the analyte of interest and stored on computer-readable media.

    摘要翻译： 确定性采矿感兴趣的样品中的分析物的量的计算机实现的方法，所述方法包括（a）形成的扩增产物从与感兴趣的分析物，（b）收集信号数据用的信号与放大的形成测量装置兼 产品，所收集的信号数据包括：由所述样品发射的信号的电平的周期性测量，worin由样品发射的信号的电平对应于扩增产物通过样品，（c）中形成的量在存储所收集的信号数据 计算机可读介质; 以及使用与在算法，其编程的计算机（d）中，当由计算机执行时，被配置为使访问存储在步骤（c）的数据，并调整在步骤（b）以去除背景收集的数据的计算机 到具有比所关注的分析物的存在其他源相关联的测量水平的贡献，由测得的最大电平，调节到去除背景的贡献将经调整的数据归一化在步骤（d）调整的数据（1），近似的曲线 通过结合存储在计算机可读介质中的预定的低和结合的预定的高存储在计算机可读介质上，在在哪个曲线，在步骤（d）中的逼近出苗时间确定性矿之间的调整，归一化的数据的一个部分（3）的杂交 预定阈值水平的数据和确定的矿通过比较在步骤（d）开采的出现时间的确定性的感兴趣的分析物的量（4）与阈值时间确定开采的中的分析物的已知量的 terest和存储在计算机可读介质上。

    Reagents methodsand kits for use in deactivating nucleic acids
    80.
    发明公开
    Reagents methodsand kits for use in deactivating nucleic acids 有权
    Reagentien und Verfahren zur Deaktivierung vonNukleinsäuren

    公开(公告)号：EP2286845A1

    公开(公告)日：2011-02-23

    申请号：EP10011173.1

    申请日：2005-03-04

    申请人： Gen-Probe Incorporated

    发明人： Nelson, Nornan, C. ,  Browne, Kenneth, A. ,  Dai, Lizhong ,  Russell, James ,  Flipowsky, Mark, E. ,  Kaminsky, Margarita B. ,  Kacian, Daniel B.

    IPC分类号： A61L2/16 ,  C12Q1/68 ,  C11D3/395

    CPC分类号： C12Q1/68 ,  A61L2/186 ,  C11D3/3956 ,  C12Q1/6848

    摘要： A formulation for use in preventing nucleic acids from acting as templates in an amplification reaction when the solution is combined with a nucleic acid deactivating agent, the formulation comprising a corrosion-inhibiting agent, a wetting agent, and a solubilizing agent, wherein each of said agents remains substantially in solution at 22°C, and wherein each agent is present in an amount that when the formulation is combined with an amount of a deactivating agent sufficient for the combined formulation to substantially deactivate nucleic acids, the corrosion-inhibiting agent reduces the corrosive properties of the deactivating agent, the wetting agent improves the dispersion properties of the deactivating agent on a solid surface and/or increases the solubility of the deactivating agent and/or other material that may be present on a solid surface or in a formulation, and the solubilizing agent increases the solubility of at least one of the deactivating agent, the corrosion-inhibiting agent, and the wetting agent.

    摘要翻译： 一种用于在将溶液与核酸减活剂组合时用于防止核酸作为扩增反应中的模板的制剂，所述制剂包含腐蚀抑制剂，润湿剂和增溶剂，其中所述 试剂在22℃下基本上保持在溶液中，并且其中每种试剂的存在量是当将制剂与足以使组合制剂基本上使核酸失活的量的减活化剂的量组合时，腐蚀抑制剂减少 钝化剂的腐蚀性质，润湿剂改善钝化剂在固体表面上的分散性能和/或增加可能存在于固体表面或制剂上的失活剂和/或其它材料的溶解度， 并且增溶剂增加至少一种钝化剂，腐蚀抑制剂的溶解度 g剂和润湿剂。